BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060978.seq
(685 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC36.10 |||mitochondrial intermembrane space protein sorting p... 75 1e-14
SPAP8A3.10 |||mitochondrial intermembrane space protein sorting ... 42 6e-05
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 27 2.5
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 26 4.4
SPAC21E11.07 ||SPAC2C4.01|glycine cleavage T-protein|Schizosacch... 26 5.8
SPBC28F2.10c |kap1||chromatin remodeling complex subunit Ngg1 |S... 25 7.7
>SPBC36.10 |||mitochondrial intermembrane space protein sorting
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 74.9 bits (176), Expect = 1e-14
Identities = 39/90 (43%), Positives = 54/90 (60%)
Frame = +3
Query: 258 DGVLHTHRLVSSKWFFPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHYIAV 437
+GVL+T RL++ PRW LI A+ CY E S V+ R +TL T+NLTF + V
Sbjct: 46 NGVLYTERLITCHQALPRWILKLIDGAQDCYIRETSYVDLKARTLTLLTSNLTFSDRLRV 105
Query: 438 DETVRYTPHPSDSSKTLLKQEAVVLYKVCL 527
DETV Y+PHP + T+ +QEA + C+
Sbjct: 106 DETVTYSPHP-ELEATVFQQEARIEALACM 134
Score = 47.2 bits (107), Expect = 2e-06
Identities = 21/43 (48%), Positives = 28/43 (65%)
Frame = +1
Query: 127 MKIWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIGTDVVERKV 255
MKI+ S H F +P+E V+ A W+KYPN VI D ++RKV
Sbjct: 1 MKIFESCHLFQYPFEQVSAAHWQKYPNEHATHVIAVDTLDRKV 43
>SPAP8A3.10 |||mitochondrial intermembrane space protein sorting
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 171
Score = 42.3 bits (95), Expect = 6e-05
Identities = 24/78 (30%), Positives = 41/78 (52%)
Frame = +3
Query: 258 DGVLHTHRLVSSKWFFPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHYIAV 437
+G L+T RL+ + PRWA L+ K Y E S ++P ++++ +T NL + V
Sbjct: 47 EGRLYTERLLVKQGRLPRWASDLLNVNK-SYILERSVIDPSKQELKSETFNLDHVKILRV 105
Query: 438 DETVRYTPHPSDSSKTLL 491
E R+ + SKT++
Sbjct: 106 IEYSRFIQSSENCSKTIV 123
Score = 36.7 bits (81), Expect = 0.003
Identities = 17/41 (41%), Positives = 23/41 (56%)
Frame = +1
Query: 133 IWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIGTDVVERKV 255
I T + N W TV+ A +YPNP + V+ DV+ER V
Sbjct: 4 ICTDKTELNASWNTVSSAWLTRYPNPYSLHVVSADVLERYV 44
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 27.1 bits (57), Expect = 2.5
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -2
Query: 444 FHQQQCN-DRMLGLLFLKSSVAVLGLLLISH*HN 346
FH Q N D++L + FL V ++G+L +H HN
Sbjct: 443 FHPPQMNTDQLLLVSFLGLVVNLVGILAFNHGHN 476
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 26.2 bits (55), Expect = 4.4
Identities = 16/61 (26%), Positives = 28/61 (45%)
Frame = +2
Query: 488 TKTRSCSTVQGVPLSSYMEDLLTNKISLNAGQRKASYRMGYRGSSTLKLRELASSACKST 667
TK R+ ST+Q P+S ++ L T+ + K + + + + E A S+ K
Sbjct: 1587 TKLRAISTIQRFPVSEWVSKLDTHVRNCIKFSHKQNLEEDFIHEPVIDVDEFAISSSKDI 1646
Query: 668 D 670
D
Sbjct: 1647 D 1647
>SPAC21E11.07 ||SPAC2C4.01|glycine cleavage
T-protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 325
Score = 25.8 bits (54), Expect = 5.8
Identities = 13/41 (31%), Positives = 24/41 (58%), Gaps = 4/41 (9%)
Frame = +2
Query: 464 SFRFLKN----ITKTRSCSTVQGVPLSSYMEDLLTNKISLN 574
SF+ +N + ++S V+GV +++ L TNKI+L+
Sbjct: 14 SFQLFRNYSVCLNSSKSLIRVEGVDAVKFLQGLTTNKITLD 54
>SPBC28F2.10c |kap1||chromatin remodeling complex subunit Ngg1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 7.7
Identities = 12/21 (57%), Positives = 14/21 (66%), Gaps = 2/21 (9%)
Frame = +2
Query: 359 DIRS--KPNTATDDFKNNKPN 415
D+RS ATDDF +NKPN
Sbjct: 209 DVRSLVAGTPATDDFSHNKPN 229
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,975,504
Number of Sequences: 5004
Number of extensions: 65277
Number of successful extensions: 157
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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