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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060978.seq
         (685 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g13070.1 68418.m01498 MSF1-like family protein similar to px1...    49   3e-06
At4g22790.1 68417.m03289 MATE efflux family protein contains Pfa...    29   3.8  
At2g36580.1 68415.m04486 pyruvate kinase, putative similar to py...    28   5.0  
At3g02500.1 68416.m00238 expressed protein                             27   8.8  
At2g31920.1 68415.m03899 expressed protein                             27   8.8  

>At5g13070.1 68418.m01498 MSF1-like family protein similar to px19
           [Gallus gallus] GI:969170; contains Pfam profile
           PF04707: MSF1-like conserved region
          Length = 183

 Score = 49.2 bits (112), Expect = 3e-06
 Identities = 30/87 (34%), Positives = 46/87 (52%), Gaps = 1/87 (1%)
 Frame = +3

Query: 261 GVLHTHRLVSSKWFFPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHYIAVD 440
           G LHT R ++     P +   +IG   IC+  E + V+   R M L T N++   +I V+
Sbjct: 53  GKLHTTRALTIHAPGPWFLHRIIGQ-DICHCVESTVVDGKSRSMQLTTKNISLKKFIEVE 111

Query: 441 ETVRYTPHPSD-SSKTLLKQEAVVLYK 518
           E +RY PHP + S+ T+  QE  +  K
Sbjct: 112 ERIRYDPHPDNPSAWTVCSQETSIRIK 138



 Score = 44.4 bits (100), Expect = 7e-05
 Identities = 17/47 (36%), Positives = 28/47 (59%), Gaps = 4/47 (8%)
 Frame = +1

Query: 127 MKIWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIG----TDVVERKV 255
           +K +  EH + HPWE V+ A+WRK+ +P N  ++      D + RK+
Sbjct: 2   VKAYRQEHVYKHPWERVSAASWRKFADPENKRILSHILEVDTLNRKL 48


>At4g22790.1 68417.m03289 MATE efflux family protein contains Pfam
           profile PF01554: Uncharacterized membrane protein family
          Length = 491

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 8/19 (42%), Positives = 12/19 (63%)
 Frame = -2

Query: 219 WIHRIRIFSPCCLSNCFPW 163
           W+  I++  PCCL+ C  W
Sbjct: 253 WLTLIKLSGPCCLTVCLEW 271


>At2g36580.1 68415.m04486 pyruvate kinase, putative similar to
           pyruvate kinase, cytosolic isozyme [Glycine max]
           SWISS-PROT:Q42806
          Length = 527

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 15/46 (32%), Positives = 20/46 (43%)
 Frame = -3

Query: 248 LSTTSVPMTAGFIGLGYFRHAA*ATVSHGWLNVCSDVQIFMFFSNI 111
           +ST  V     F+ L Y RHA     +   LN C D+     F+ I
Sbjct: 212 ISTWGVQNKIDFLSLSYCRHAEDVRQARELLNSCGDLSQTQIFAKI 257


>At3g02500.1 68416.m00238 expressed protein 
          Length = 278

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 13/37 (35%), Positives = 20/37 (54%)
 Frame = -1

Query: 541 HVTTERHTLYSTTASCFSNVFEESEGCGVYRTVSSTA 431
           H+T E  +    +ASC  +  E+ + C    T+SSTA
Sbjct: 20  HLTREDQSSRRFSASCIRSFREDHKSCTTNVTISSTA 56


>At2g31920.1 68415.m03899 expressed protein
          Length = 585

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 17/59 (28%), Positives = 27/59 (45%), Gaps = 2/59 (3%)
 Frame = +3

Query: 372 NPIQRQMTLKTTNLTFCHYIAVDETVRYTPHPSDSSKTLLK--QEAVVLYKVCLSVVTW 542
           +PI + +  +T  L   + +     +  T  P D  K+L K     V L+KV  S +TW
Sbjct: 239 SPISKHLNCETPALRSRYVVKPASPISVTRSPKDGIKSLSKAVTPPVALFKVPSSHMTW 297


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,211,495
Number of Sequences: 28952
Number of extensions: 329070
Number of successful extensions: 739
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 732
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 739
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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