Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060968.seq
(685 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q9VUR4 Cluster: Ubiquitin carrier protein; n=7; Eukaryo... 86 7e-16
UniRef50_Q712K3 Cluster: Ubiquitin-conjugating enzyme E2 R2; n=6... 60 7e-08
UniRef50_Q5DD88 Cluster: Ubiquitin carrier protein; n=2; Schisto... 54 3e-06
UniRef50_Q5KEU0 Cluster: Ubiquitin conjugating enzyme, putative;... 42 0.014
UniRef50_Q8IH42 Cluster: Ubiquitin carrier protein; n=15; Fungi/... 42 0.019
UniRef50_A0C0C6 Cluster: Chromosome undetermined scaffold_14, wh... 40 0.043
UniRef50_UPI0000498417 Cluster: ubiquitin-conjugating enzyme; n=... 40 0.075
UniRef50_P62253 Cluster: Ubiquitin-conjugating enzyme E2 G1; n=6... 38 0.17
UniRef50_P60604 Cluster: Ubiquitin-conjugating enzyme E2 G2; n=8... 38 0.23
UniRef50_Q54J28 Cluster: Putative uncharacterized protein; n=1; ... 38 0.30
UniRef50_A2AX46 Cluster: Ubiquitin carrier protein; n=2; Eukaryo... 37 0.40
UniRef50_Q9N490 Cluster: Ubiquitin conjugating enzyme protein 15... 37 0.40
UniRef50_A7SJX4 Cluster: Predicted protein; n=1; Nematostella ve... 37 0.40
UniRef50_Q9UTN8 Cluster: Ubiquitin conjugating enzyme Ubc14; n=1... 37 0.40
UniRef50_P27949 Cluster: Ubiquitin-conjugating enzyme E2-21 kDa;... 37 0.53
UniRef50_Q57XM0 Cluster: Ubiquitin carrier protein; n=1; Trypano... 35 1.6
UniRef50_O97292 Cluster: Putative uncharacterized protein MAL3P7... 33 4.9
UniRef50_Q7SCR0 Cluster: Predicted protein; n=1; Neurospora cras... 33 4.9
UniRef50_Q8BXK3 Cluster: 10 days neonate medulla oblongata cDNA,... 33 6.5
UniRef50_A6SKB5 Cluster: Putative uncharacterized protein; n=2; ... 33 6.5
UniRef50_Q6E689 Cluster: Ubiquitin carrier protein; n=1; Antonos... 33 8.6
UniRef50_O42646 Cluster: Ubiquitin-conjugating enzyme E2 6; n=2;... 33 8.6
>UniRef50_Q9VUR4 Cluster: Ubiquitin carrier protein; n=7;
Eukaryota|Rep: Ubiquitin carrier protein - Drosophila
melanogaster (Fruit fly)
Length = 341
Score = 86.2 bits (204), Expect = 7e-16
Identities = 42/67 (62%), Positives = 49/67 (73%)
Frame = +1
Query: 379 TVPTSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSRAHM 558
T P+SSA+RALA+EYKSLQEEPVEGFRVKL+ +DNLFEWEVAI + +AHM
Sbjct: 57 TTPSSSAVRALAMEYKSLQEEPVEGFRVKLINDDNLFEWEVAIFGPPDTLYQGGYFKAHM 116
Query: 559 XFPPGLP 579
FP P
Sbjct: 117 KFPHDYP 123
Score = 45.6 bits (103), Expect = 0.001
Identities = 18/18 (100%), Positives = 18/18 (100%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
IRFLTKVWHPNVYENGDL
Sbjct: 129 IRFLTKVWHPNVYENGDL 146
Score = 40.3 bits (90), Expect = 0.043
Identities = 25/56 (44%), Positives = 27/56 (48%)
Frame = +3
Query: 510 GPPDTLYQGGYFKGTHXIPPWTTRIHHHPLGF*LKSGIQMYMKTETFGISILHPPV 677
GPPDTLYQGGYFK H P + F K + ISILHPPV
Sbjct: 101 GPPDTLYQGGYFKA-HMKFPHDYPYSPPSIRFLTKVWHPNVYENGDLCISILHPPV 155
>UniRef50_Q712K3 Cluster: Ubiquitin-conjugating enzyme E2 R2; n=61;
Eumetazoa|Rep: Ubiquitin-conjugating enzyme E2 R2 - Homo
sapiens (Human)
Length = 238
Score = 59.7 bits (138), Expect = 7e-08
Identities = 30/63 (47%), Positives = 40/63 (63%)
Frame = +1
Query: 391 SSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSRAHMXFPP 570
+S+ +AL LE KSLQEEPVEGFR+ L+ E +L+ WEVAI + +AH+ FP
Sbjct: 7 TSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPI 66
Query: 571 GLP 579
P
Sbjct: 67 DYP 69
Score = 41.1 bits (92), Expect = 0.024
Identities = 14/17 (82%), Positives = 17/17 (100%)
Frame = +2
Query: 599 RFLTKVWHPNVYENGDL 649
RFLTK+WHPN+YENGD+
Sbjct: 76 RFLTKMWHPNIYENGDV 92
Score = 35.9 bits (79), Expect = 0.92
Identities = 23/56 (41%), Positives = 26/56 (46%)
Frame = +3
Query: 510 GPPDTLYQGGYFKGTHXIPPWTTRIHHHPLGF*LKSGIQMYMKTETFGISILHPPV 677
GPP+TLY+GGYFK H P F K + ISILHPPV
Sbjct: 47 GPPNTLYEGGYFKA-HIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPV 101
>UniRef50_Q5DD88 Cluster: Ubiquitin carrier protein; n=2;
Schistosoma japonicum|Rep: Ubiquitin carrier protein -
Schistosoma japonicum (Blood fluke)
Length = 289
Score = 54.0 bits (124), Expect = 3e-06
Identities = 27/70 (38%), Positives = 41/70 (58%)
Frame = +1
Query: 370 MSNTVPTSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSR 549
M++ P +SA++AL E K L E PVEGF+V + ++NLF W+VAI + + +
Sbjct: 1 MASRKPNASAVKALQKELKDLNEFPVEGFKVMVSEDENLFVWDVAIFGPPMTLYEGGYFK 60
Query: 550 AHMXFPPGLP 579
A + FP P
Sbjct: 61 ARLCFPDDYP 70
>UniRef50_Q5KEU0 Cluster: Ubiquitin conjugating enzyme, putative;
n=1; Filobasidiella neoformans|Rep: Ubiquitin
conjugating enzyme, putative - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 209
Score = 41.9 bits (94), Expect = 0.014
Identities = 23/70 (32%), Positives = 37/70 (52%)
Frame = +1
Query: 373 SNTVPTSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSRA 552
S+T +S LR +E LQ+ PV+GF L+ +DN+ EW++ I + +A
Sbjct: 35 SSTANSSLILRKQLME---LQKHPVDGFSAGLVDDDNMLEWDIVIMGPVDTLWEGAILKA 91
Query: 553 HMXFPPGLPV 582
+ FPP P+
Sbjct: 92 RLIFPPEYPL 101
>UniRef50_Q8IH42 Cluster: Ubiquitin carrier protein; n=15;
Fungi/Metazoa group|Rep: Ubiquitin carrier protein -
Drosophila melanogaster (Fruit fly)
Length = 180
Score = 41.5 bits (93), Expect = 0.019
Identities = 25/74 (33%), Positives = 38/74 (51%), Gaps = 5/74 (6%)
Frame = +1
Query: 376 NTVPTS--SALRALAL---EYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVD 540
N +P+S S L+A L + LQ PVEGF L+ + ++F+WEV I +
Sbjct: 6 NKIPSSDMSELQASLLLNRQLSELQRHPVEGFSAGLVSDSDIFKWEVVIIGPPDTLYEGG 65
Query: 541 TSRAHMXFPPGLPV 582
+AH+ FP P+
Sbjct: 66 FFKAHLIFPKEYPL 79
>UniRef50_A0C0C6 Cluster: Chromosome undetermined scaffold_14, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_14,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 150
Score = 40.3 bits (90), Expect = 0.043
Identities = 14/39 (35%), Positives = 27/39 (69%)
Frame = +1
Query: 394 SALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAIS 510
S++ + E ++LQ+EP+ GF + +L ++NLF W++ S
Sbjct: 2 SSISRIKKELQNLQDEPLNGFLISILDDNNLFHWKICFS 40
>UniRef50_UPI0000498417 Cluster: ubiquitin-conjugating enzyme; n=1;
Entamoeba histolytica HM-1:IMSS|Rep:
ubiquitin-conjugating enzyme - Entamoeba histolytica
HM-1:IMSS
Length = 219
Score = 39.5 bits (88), Expect = 0.075
Identities = 15/36 (41%), Positives = 24/36 (66%)
Frame = +1
Query: 394 SALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEV 501
SA + + YKS+ ++P+EG V+ EDN+FEW +
Sbjct: 2 SAAKFIQSNYKSIIKDPLEGIYVEYASEDNIFEWNI 37
>UniRef50_P62253 Cluster: Ubiquitin-conjugating enzyme E2 G1; n=66;
Eukaryota|Rep: Ubiquitin-conjugating enzyme E2 G1 - Homo
sapiens (Human)
Length = 170
Score = 38.3 bits (85), Expect = 0.17
Identities = 18/58 (31%), Positives = 29/58 (50%)
Frame = +1
Query: 409 LALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSRAHMXFPPGLPV 582
L + L + PVEGF L+ +++L+ WEV I + +AH+ FP P+
Sbjct: 10 LRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPL 67
Score = 33.5 bits (73), Expect = 4.9
Identities = 10/18 (55%), Positives = 17/18 (94%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
++F+T++WHPNV +NGD+
Sbjct: 72 MKFITEIWHPNVDKNGDV 89
>UniRef50_P60604 Cluster: Ubiquitin-conjugating enzyme E2 G2; n=80;
Eukaryota|Rep: Ubiquitin-conjugating enzyme E2 G2 - Homo
sapiens (Human)
Length = 165
Score = 37.9 bits (84), Expect = 0.23
Identities = 21/63 (33%), Positives = 28/63 (44%)
Frame = +1
Query: 394 SALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSRAHMXFPPG 573
+AL+ L EYK L P EG + E+N FEWE I + A + FP
Sbjct: 4 TALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLD 63
Query: 574 LPV 582
P+
Sbjct: 64 YPL 66
>UniRef50_Q54J28 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 41
Score = 37.5 bits (83), Expect = 0.30
Identities = 14/33 (42%), Positives = 24/33 (72%)
Frame = +1
Query: 403 RALALEYKSLQEEPVEGFRVKLLGEDNLFEWEV 501
+ LA + S+ +PVEGF V+L+ + +L+EW+V
Sbjct: 4 KLLANQLNSIMNDPVEGFTVELVNDSSLYEWKV 36
>UniRef50_A2AX46 Cluster: Ubiquitin carrier protein; n=2;
Eukaryota|Rep: Ubiquitin carrier protein - Guillardia
theta (Cryptomonas phi)
Length = 201
Score = 37.1 bits (82), Expect = 0.40
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
++FLT +WHPNVY NGD+
Sbjct: 106 MKFLTPIWHPNVYSNGDV 123
Score = 35.9 bits (79), Expect = 0.92
Identities = 21/75 (28%), Positives = 34/75 (45%), Gaps = 3/75 (4%)
Frame = +1
Query: 364 LTMSNTVPTSSALRA---LALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIK 534
L M+ + + LR+ L ++ + LQE P E F L+ + N+ EW + IS +
Sbjct: 26 LAMAPAANSPTRLRSSQILRMQLRELQENPSELFSAGLVDDSNMLEWNICISGPPDSLYE 85
Query: 535 VDTSRAHMXFPPGLP 579
A + FP P
Sbjct: 86 GGIFSARLSFPENYP 100
>UniRef50_Q9N490 Cluster: Ubiquitin conjugating enzyme protein 15;
n=2; Caenorhabditis|Rep: Ubiquitin conjugating enzyme
protein 15 - Caenorhabditis elegans
Length = 218
Score = 37.1 bits (82), Expect = 0.40
Identities = 16/40 (40%), Positives = 25/40 (62%)
Frame = +1
Query: 388 TSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAI 507
+SSA+R L +Y L ++PV+G + L EDN+ EW +
Sbjct: 17 SSSAVRRLQKDYAKLMQDPVDGIKA-LPNEDNILEWHYCL 55
>UniRef50_A7SJX4 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 139
Score = 37.1 bits (82), Expect = 0.40
Identities = 16/52 (30%), Positives = 29/52 (55%)
Frame = +1
Query: 424 KSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSRAHMXFPPGLP 579
+ LQ++PVEGF L +++L++WE+ + + + +A M FP P
Sbjct: 19 QQLQKKPVEGFSAGLFDDEDLYKWEIMVVGPPGTYYEEGYFKASMVFPKEYP 70
>UniRef50_Q9UTN8 Cluster: Ubiquitin conjugating enzyme Ubc14; n=1;
Schizosaccharomyces pombe|Rep: Ubiquitin conjugating
enzyme Ubc14 - Schizosaccharomyces pombe (Fission yeast)
Length = 155
Score = 37.1 bits (82), Expect = 0.40
Identities = 19/42 (45%), Positives = 26/42 (61%)
Frame = +1
Query: 370 MSNTVPTSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEW 495
M++ P+SS R L EY L+E P+ RV L+ +DNLF W
Sbjct: 1 MASASPSSS--RRLTKEYSDLREHPIPDIRVNLV-DDNLFHW 39
>UniRef50_P27949 Cluster: Ubiquitin-conjugating enzyme E2-21 kDa;
n=2; African swine fever virus|Rep:
Ubiquitin-conjugating enzyme E2-21 kDa - African swine
fever virus (strain BA71V) (ASFV)
Length = 215
Score = 36.7 bits (81), Expect = 0.53
Identities = 19/59 (32%), Positives = 30/59 (50%)
Frame = +1
Query: 403 RALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSRAHMXFPPGLP 579
R L EY+ L E P E F++ + E+N+ EW+V + + +A + FPP P
Sbjct: 4 RFLIAEYRHLIENPSENFKIS-VNENNITEWDVILRGPPDTLYEGGLFKAKVAFPPEYP 61
Score = 34.3 bits (75), Expect = 2.8
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = +3
Query: 510 GPPDTLYQGGYFKGTHXIPP 569
GPPDTLY+GG FK PP
Sbjct: 39 GPPDTLYEGGLFKAKVAFPP 58
>UniRef50_Q57XM0 Cluster: Ubiquitin carrier protein; n=1;
Trypanosoma brucei|Rep: Ubiquitin carrier protein -
Trypanosoma brucei
Length = 226
Score = 35.1 bits (77), Expect = 1.6
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
+RF TK++HPNVY NGD+
Sbjct: 130 VRFTTKIFHPNVYGNGDI 147
>UniRef50_O97292 Cluster: Putative uncharacterized protein MAL3P7.22;
n=1; Plasmodium falciparum 3D7|Rep: Putative
uncharacterized protein MAL3P7.22 - Plasmodium falciparum
(isolate 3D7)
Length = 2706
Score = 33.5 bits (73), Expect = 4.9
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = -2
Query: 147 TLKSRRNNRPKAHDQLNNNEKAKHRSTKRNPKK 49
T + RNNR ++ NNN K++ RST+ N KK
Sbjct: 2602 TSQRTRNNRRNNNNNNNNNRKSRKRSTRTNRKK 2634
>UniRef50_Q7SCR0 Cluster: Predicted protein; n=1; Neurospora
crassa|Rep: Predicted protein - Neurospora crassa
Length = 562
Score = 33.5 bits (73), Expect = 4.9
Identities = 25/86 (29%), Positives = 40/86 (46%)
Frame = -3
Query: 512 SEIATSHSNRLSSPSNLTRKPSTGSSCRLLYSNAKALRAELVGTVLDIVSYVFIQPSLPS 333
S + S+S+ ++SP LTR + SS + ++AL + L+ Y+ IQ + P
Sbjct: 125 SPVTDSNSDTMASPPVLTRTYNPPSSYHHTHHTSQALPSSLIPAP---SQYMHIQVAPPP 181
Query: 332 *TKLYKHITLELFTLSTYPPGISTRY 255
LY + +TYPP S Y
Sbjct: 182 PMSLYPSMPALSQRTTTYPPSSSYDY 207
>UniRef50_Q8BXK3 Cluster: 10 days neonate medulla oblongata cDNA,
RIKEN full-length enriched library, clone:B830003C01
product:hypothetical Lysine-rich region containing
protein, full insert sequence; n=1; Mus musculus|Rep: 10
days neonate medulla oblongata cDNA, RIKEN full-length
enriched library, clone:B830003C01 product:hypothetical
Lysine-rich region containing protein, full insert
sequence - Mus musculus (Mouse)
Length = 125
Score = 33.1 bits (72), Expect = 6.5
Identities = 23/76 (30%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Frame = -2
Query: 261 SLHNNELEILVNIASILQHPQVR----VTHFKRQPLNLC**FTLKSRRNNRPKAHDQLNN 94
SL + V+ +LQHPQV HF + +L ++N + K +Q+NN
Sbjct: 9 SLKKIHVIYFVHSFPLLQHPQVLPISLCIHFYVLSPRVKLSLSLSKKKNKKKKEKNQINN 68
Query: 93 NEKAKH-RSTKRNPKK 49
N+K + K NP+K
Sbjct: 69 NKKPNETKQNKTNPQK 84
>UniRef50_A6SKB5 Cluster: Putative uncharacterized protein; n=2;
Sclerotiniaceae|Rep: Putative uncharacterized protein -
Botryotinia fuckeliana B05.10
Length = 153
Score = 33.1 bits (72), Expect = 6.5
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = +1
Query: 397 ALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEV 501
A R +A E+K + + P+ G L+ E++L +W+V
Sbjct: 2 AARRIAKEFKEVTDNPIPGISTSLVKEEDLHQWKV 36
>UniRef50_Q6E689 Cluster: Ubiquitin carrier protein; n=1;
Antonospora locustae|Rep: Ubiquitin carrier protein -
Antonospora locustae (Nosema locustae)
Length = 155
Score = 32.7 bits (71), Expect = 8.6
Identities = 11/18 (61%), Positives = 17/18 (94%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
++F++KV+HPNVY +GDL
Sbjct: 72 VKFVSKVFHPNVYASGDL 89
>UniRef50_O42646 Cluster: Ubiquitin-conjugating enzyme E2 6; n=2;
Schizosaccharomyces pombe|Rep: Ubiquitin-conjugating
enzyme E2 6 - Schizosaccharomyces pombe (Fission yeast)
Length = 227
Score = 32.7 bits (71), Expect = 8.6
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +3
Query: 510 GPPDTLYQGGYFKGTHXIPP 569
GPPDT Y+GG + GT PP
Sbjct: 43 GPPDTPYEGGQYHGTLIFPP 62
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 708,045,847
Number of Sequences: 1657284
Number of extensions: 14435922
Number of successful extensions: 33731
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 31976
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33675
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 53305790091
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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