Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060968.seq
         (685 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    41   1e-04
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac...    37   0.002
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    33   0.051
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo...    31   0.20 
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    29   0.47 
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    28   1.4  
SPAC17G6.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||M...    27   2.5  
SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase |Schizosa...    27   3.3  
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha...    27   3.3  
SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr...    26   4.4  
SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces pombe...    26   5.8  
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki...    26   5.8  
SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    25   7.7  

>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 41.1 bits (92), Expect = 1e-04
 Identities = 20/67 (29%), Positives = 36/67 (53%)
 Frame = +1

Query: 382 VPTSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSRAHMX 561
           +P+S++ + L  + K +Q+ P +GF V L+ + ++FEWEV I        +     A + 
Sbjct: 1   MPSSASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLS 60

Query: 562 FPPGLPV 582
           FP   P+
Sbjct: 61  FPQDYPL 67



 Score = 31.9 bits (69), Expect = 0.089
 Identities = 9/18 (50%), Positives = 16/18 (88%)
 Frame = +2

Query: 596 IRFLTKVWHPNVYENGDL 649
           ++F T++WHPNV+ NG++
Sbjct: 72  MKFTTEIWHPNVHPNGEV 89


>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
           Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 155

 Score = 37.1 bits (82), Expect = 0.002
 Identities = 19/42 (45%), Positives = 26/42 (61%)
 Frame = +1

Query: 370 MSNTVPTSSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEW 495
           M++  P+SS  R L  EY  L+E P+   RV L+ +DNLF W
Sbjct: 1   MASASPSSS--RRLTKEYSDLREHPIPDIRVNLV-DDNLFHW 39


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 32.7 bits (71), Expect = 0.051
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = +3

Query: 510 GPPDTLYQGGYFKGTHXIPP 569
           GPPDT Y+GG + GT   PP
Sbjct: 43  GPPDTPYEGGQYHGTLIFPP 62


>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
           Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 30.7 bits (66), Expect = 0.20
 Identities = 15/37 (40%), Positives = 18/37 (48%)
 Frame = +1

Query: 397 ALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAI 507
           ALR L  EYK L E   +G       ED+ F W+  I
Sbjct: 6   ALRRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLI 42


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 29.5 bits (63), Expect = 0.47
 Identities = 9/18 (50%), Positives = 16/18 (88%)
 Frame = +2

Query: 596 IRFLTKVWHPNVYENGDL 649
           ++F++ ++HPNVY NG+L
Sbjct: 70  VKFVSTMFHPNVYANGEL 87


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 10/16 (62%), Positives = 14/16 (87%)
 Frame = +2

Query: 596 IRFLTKVWHPNVYENG 643
           +RFLTK++HPNV + G
Sbjct: 68  VRFLTKIYHPNVDKLG 83


>SPAC17G6.11c |||glucosidase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 636

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 15/34 (44%), Positives = 23/34 (67%)
 Frame = -3

Query: 512 SEIATSHSNRLSSPSNLTRKPSTGSSCRLLYSNA 411
           +EI + +++R +S S+ T    T SS RLLY+NA
Sbjct: 19  NEIGSMYASRDTSTSSFTNP--TDSSTRLLYNNA 50


>SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1076

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 12/31 (38%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
 Frame = +1

Query: 370 MSNTVPTS-SALRALALEYKSLQEEPVEGFR 459
           M ++VP + ++L     E+ +L E+P+EGFR
Sbjct: 22  MFHSVPQNKNSLSVCVAEFTALSEKPLEGFR 52


>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
           Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 7/18 (38%), Positives = 13/18 (72%)
 Frame = +2

Query: 596 IRFLTKVWHPNVYENGDL 649
           + F T+++HPN+  NG +
Sbjct: 67  VNFTTRIYHPNINSNGSI 84


>SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 652

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 12/42 (28%), Positives = 22/42 (52%)
 Frame = -2

Query: 144 LKSRRNNRPKAHDQLNNNEKAKHRSTKRNPKKISYVRATAAD 19
           L++R     +A  Q +N++  +    K+  KK+  +RAT  D
Sbjct: 113 LEARLKGDKEARGQNSNDQPEESDDKKKRKKKVKEIRATRLD 154


>SPBC1289.04c |pob1||Boi family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 871

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 11/40 (27%), Positives = 23/40 (57%)
 Frame = -3

Query: 527 KCIRRSEIATSHSNRLSSPSNLTRKPSTGSSCRLLYSNAK 408
           K    + +++SHS+ L+ P +   KPS  S+   ++ ++K
Sbjct: 108 KLYSNNSLSSSHSSILNGPLDSLSKPSVPSNFNSMFPSSK 147


>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase
           kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1436

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 15/47 (31%), Positives = 24/47 (51%)
 Frame = -3

Query: 551 ALEVSTLIKCIRRSEIATSHSNRLSSPSNLTRKPSTGSSCRLLYSNA 411
           AL +ST +  I   E+  +++   S  SNL   P++ SS  +   NA
Sbjct: 103 ALTISTPVDPINIDELDRAYAVSPSDTSNLLHPPTSSSSIPIPIKNA 149


>SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 681

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 18/54 (33%), Positives = 26/54 (48%)
 Frame = -3

Query: 488 NRLSSPSNLTRKPSTGSSCRLLYSNAKALRAELVGTVLDIVSYVFIQPSLPS*T 327
           N LSSPS+    P + S  R    N+ A   ++  T LD +  +  +P L S T
Sbjct: 135 NNLSSPSSSLYLPGSASYQRSNSKNSSASILQM-NTTLDDIPILLRRPGLSSYT 187


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,945,903
Number of Sequences: 5004
Number of extensions: 60910
Number of successful extensions: 169
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)