Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060968.seq
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55380.1 68416.m06151 ubiquitin-conjugating enzyme 14 (UBC14)... 38 0.008
At3g46460.1 68416.m05037 ubiquitin-conjugating enzyme 13 (UBC13)... 37 0.011
At5g59300.1 68418.m07430 ubiquitin-conjugating enzyme 7 (UBC7) E... 32 0.31
At3g17000.1 68416.m02171 ubiquitin-conjugating enzyme, putative ... 30 1.2
At5g05080.1 68418.m00539 ubiquitin-conjugating enzyme, putative ... 29 3.8
At5g25760.1 68418.m03057 ubiquitin-conjugating enzyme, putative ... 28 5.0
At5g62540.1 68418.m07849 ubiquitin-conjugating enzyme 3 (UBC3) E... 28 6.6
At2g25790.1 68415.m03095 leucine-rich repeat transmembrane prote... 28 6.6
At2g02760.1 68415.m00219 ubiquitin-conjugating enzyme 2 (UBC2) E... 28 6.6
At1g14400.2 68414.m01708 ubiquitin-conjugating enzyme 1 (UBC1) E... 28 6.6
At1g14400.1 68414.m01707 ubiquitin-conjugating enzyme 1 (UBC1) E... 28 6.6
At5g56150.2 68418.m07005 ubiquitin-conjugating enzyme, putative ... 27 8.8
At5g56150.1 68418.m07004 ubiquitin-conjugating enzyme, putative ... 27 8.8
At5g51160.1 68418.m06343 ankyrin repeat family protein contains ... 27 8.8
At5g19480.1 68418.m02321 expressed protein 27 8.8
At3g08690.1 68416.m01010 ubiquitin-conjugating enzyme 11 (UBC11)... 27 8.8
At2g36200.1 68415.m04444 kinesin motor protein-related 27 8.8
At1g78870.2 68414.m09194 ubiquitin-conjugating enzyme, putative ... 27 8.8
At1g78870.1 68414.m09193 ubiquitin-conjugating enzyme, putative ... 27 8.8
At1g64230.1 68414.m07276 ubiquitin-conjugating enzyme, putative ... 27 8.8
At1g16890.2 68414.m02044 ubiquitin-conjugating enzyme, putative ... 27 8.8
At1g16890.1 68414.m02043 ubiquitin-conjugating enzyme, putative ... 27 8.8
>At3g55380.1 68416.m06151 ubiquitin-conjugating enzyme 14 (UBC14)
E2; UbcAT3; identical to gi:2129757, S46656
Length = 167
Score = 37.5 bits (83), Expect = 0.008
Identities = 20/58 (34%), Positives = 29/58 (50%)
Frame = +1
Query: 409 LALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSRAHMXFPPGLPV 582
L + K L ++PV+GF L+ E N+F+W V+I + A M FP PV
Sbjct: 10 LQKQLKDLCKKPVDGFSAGLVDEKNVFQWSVSIMGPPDTLYEGGFFNAIMSFPENYPV 67
Score = 28.3 bits (60), Expect = 5.0
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENG 643
+ F +++WHPNVY +G
Sbjct: 72 VTFTSEMWHPNVYSDG 87
>At3g46460.1 68416.m05037 ubiquitin-conjugating enzyme 13 (UBC13)
E2; identical to gi:992706
Length = 166
Score = 37.1 bits (82), Expect = 0.011
Identities = 22/63 (34%), Positives = 28/63 (44%)
Frame = +1
Query: 391 SSALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAISDRRIHFIKVDTSRAHMXFPP 570
S A L + K L + PV+GF L+ E N+FEW V I + A M FP
Sbjct: 3 SQACLLLQKQLKDLCKHPVDGFSAGLVDEKNIFEWSVTIIGPPDTLYEGGFFYAIMSFPQ 62
Query: 571 GLP 579
P
Sbjct: 63 NYP 65
Score = 30.3 bits (65), Expect = 1.2
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENG 643
+RF + +WHPNVY +G
Sbjct: 71 VRFTSDIWHPNVYPDG 86
>At5g59300.1 68418.m07430 ubiquitin-conjugating enzyme 7 (UBC7) E2;
identical to gi:992703, SP:P42747
Length = 198
Score = 32.3 bits (70), Expect = 0.31
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +3
Query: 507 LGPPDTLYQGGYFKGTHXIP 566
+GPPDTLY+GG+F P
Sbjct: 74 IGPPDTLYEGGFFNAIMTFP 93
Score = 29.9 bits (64), Expect = 1.6
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENG 643
+RF + +WHPNVY +G
Sbjct: 103 VRFTSDMWHPNVYSDG 118
>At3g17000.1 68416.m02171 ubiquitin-conjugating enzyme, putative
similar to Non-Canonical UBiquitin Conjugating Enzyme 1
(NCUBE1) from [Gallus gallus] GI:7362937, [Mus musculus]
GI:7363050, [Homo sapiens] GI:7362973; contains Pfam
profile PF00179: Ubiquitin-conjugating enzyme
Length = 309
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/37 (37%), Positives = 23/37 (62%)
Frame = +1
Query: 397 ALRALALEYKSLQEEPVEGFRVKLLGEDNLFEWEVAI 507
A++ + E K +Q P + F + L E+N+FEW+ AI
Sbjct: 12 AVKRILQEVKEMQANPSDDF-MSLPLEENIFEWQFAI 47
>At5g05080.1 68418.m00539 ubiquitin-conjugating enzyme, putative
similar to SP|Q16763 Ubiquitin-conjugating enzyme E2-24
kDa (EC 6.3.2.19) (Ubiquitin- protein ligase) (Ubiquitin
carrier protein) {Homo sapiens}; contains Pfam profile
PF00179: Ubiquitin-conjugating enzyme
Length = 251
Score = 28.7 bits (61), Expect = 3.8
Identities = 9/16 (56%), Positives = 14/16 (87%)
Frame = +2
Query: 602 FLTKVWHPNVYENGDL 649
F+TK++HPNV NG++
Sbjct: 78 FMTKIFHPNVASNGEI 93
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 373 SNTVPTSSALRALALEYKSLQEEPVEGFRVKLLGED 480
SN + ++ LA E KSL E P +G +V + ED
Sbjct: 3 SNENLPPNVIKQLAKELKSLDESPPDGIKVVVNDED 38
>At5g25760.1 68418.m03057 ubiquitin-conjugating enzyme, putative
similar to SP|O60015 Ubiquitin-conjugating enzyme E2-21
kDa (EC 6.3.2.19) (Ubiquitin-protein ligase) (Ubiquitin
carrier protein) {Pichia angusta}; contains Pfam profile
PF00179: Ubiquitin-conjugating enzyme
Length = 157
Score = 28.3 bits (60), Expect = 5.0
Identities = 10/19 (52%), Positives = 17/19 (89%), Gaps = 1/19 (5%)
Frame = +2
Query: 596 IRFLTKVWHPNV-YENGDL 649
+RFLTK++HPNV ++ G++
Sbjct: 71 VRFLTKIFHPNVHFKTGEI 89
>At5g62540.1 68418.m07849 ubiquitin-conjugating enzyme 3 (UBC3) E2;
identical to gi:431261, SP:P42746
Length = 150
Score = 27.9 bits (59), Expect = 6.6
Identities = 7/18 (38%), Positives = 16/18 (88%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
+RF+++++HPN+Y +G +
Sbjct: 70 VRFVSRMFHPNIYADGSI 87
>At2g25790.1 68415.m03095 leucine-rich repeat transmembrane protein
kinase, putative
Length = 960
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -2
Query: 270 HFNSLHNNELEILVNIASILQHP 202
+F+ LH NELE+L++ S +Q P
Sbjct: 23 NFSCLHANELELLLSFKSSIQDP 45
>At2g02760.1 68415.m00219 ubiquitin-conjugating enzyme 2 (UBC2) E2;
identical to gi:2689242, SP:P42745
Length = 152
Score = 27.9 bits (59), Expect = 6.6
Identities = 7/18 (38%), Positives = 16/18 (88%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
+RF+++++HPN+Y +G +
Sbjct: 70 VRFVSRMFHPNIYADGSI 87
>At1g14400.2 68414.m01708 ubiquitin-conjugating enzyme 1 (UBC1) E2;
identical to gi:431259, SP:P25865
Length = 152
Score = 27.9 bits (59), Expect = 6.6
Identities = 7/18 (38%), Positives = 16/18 (88%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
+RF+++++HPN+Y +G +
Sbjct: 70 VRFVSRMFHPNIYADGSI 87
>At1g14400.1 68414.m01707 ubiquitin-conjugating enzyme 1 (UBC1) E2;
identical to gi:431259, SP:P25865
Length = 152
Score = 27.9 bits (59), Expect = 6.6
Identities = 7/18 (38%), Positives = 16/18 (88%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
+RF+++++HPN+Y +G +
Sbjct: 70 VRFVSRMFHPNIYADGSI 87
>At5g56150.2 68418.m07005 ubiquitin-conjugating enzyme, putative
strong similarity to ubiquitin-conjugating enzyme UBC2
[Mesembryanthemum crystallinum] GI:5762457, UBC4 [Pisum
sativum] GI:456568; contains Pfam profile PF00179:
Ubiquitin-conjugating enzyme
Length = 148
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
+ F TKV+HPN+ NG +
Sbjct: 67 VAFRTKVYHPNINSNGSI 84
>At5g56150.1 68418.m07004 ubiquitin-conjugating enzyme, putative
strong similarity to ubiquitin-conjugating enzyme UBC2
[Mesembryanthemum crystallinum] GI:5762457, UBC4 [Pisum
sativum] GI:456568; contains Pfam profile PF00179:
Ubiquitin-conjugating enzyme
Length = 148
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
+ F TKV+HPN+ NG +
Sbjct: 67 VAFRTKVYHPNINSNGSI 84
>At5g51160.1 68418.m06343 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 442
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 2/51 (3%)
Frame = -1
Query: 250 QRTRNFGKHSIDTAASTSTC--HTLQTSTLKSLLIIYVEK*TKQQTQSTRS 104
QR R+ G +++ STSTC T+++ + K L+ + K + RS
Sbjct: 222 QRGRDIGTTNVERTTSTSTCQERTMKSQSHKELVKYFTFKKHRDSPSEARS 272
>At5g19480.1 68418.m02321 expressed protein
Length = 207
Score = 27.5 bits (58), Expect = 8.8
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = -2
Query: 141 KSRRNNRPKAHDQLNNNEKAKHRSTK 64
K R+NN + D + ++K+KH+S+K
Sbjct: 175 KKRKNNGTEDADDVQRHKKSKHKSSK 200
>At3g08690.1 68416.m01010 ubiquitin-conjugating enzyme 11 (UBC11)
E2; identical to gi:12643427, SP:P35134
Length = 148
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
+ F TKV+HPN+ NG +
Sbjct: 67 VSFKTKVYHPNINSNGSI 84
>At2g36200.1 68415.m04444 kinesin motor protein-related
Length = 1056
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/51 (29%), Positives = 25/51 (49%)
Frame = -3
Query: 551 ALEVSTLIKCIRRSEIATSHSNRLSSPSNLTRKPSTGSSCRLLYSNAKALR 399
A+ + TL++ R + S + + + P LTR P S + YSN + R
Sbjct: 971 AMPIETLVEEFRENNSYESFATKETKPQQLTRSPL--SQLKFSYSNRSSQR 1019
>At1g78870.2 68414.m09194 ubiquitin-conjugating enzyme, putative
nearly identical to ubiquitin-conjugating enzyme E2
[Catharanthus roseus] GI:5381319; contains Pfam profile
PF00179: Ubiquitin-conjugating enzyme
Length = 153
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/16 (56%), Positives = 14/16 (87%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENG 643
+RFLTK++HPN+ + G
Sbjct: 71 VRFLTKIYHPNIDKLG 86
>At1g78870.1 68414.m09193 ubiquitin-conjugating enzyme, putative
nearly identical to ubiquitin-conjugating enzyme E2
[Catharanthus roseus] GI:5381319; contains Pfam profile
PF00179: Ubiquitin-conjugating enzyme
Length = 112
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/16 (56%), Positives = 14/16 (87%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENG 643
+RFLTK++HPN+ + G
Sbjct: 71 VRFLTKIYHPNIDKLG 86
>At1g64230.1 68414.m07276 ubiquitin-conjugating enzyme, putative
identical or nearly so to Ubiquitin-conjugating enzymes
SP|P35132, SP|P35131, SP|P35133 from {Arabidopsis
thaliana}; contains Pfam profile PF00179:
Ubiquitin-conjugating enzyme
Length = 148
Score = 27.5 bits (58), Expect = 8.8
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENGDL 649
+ F TKV+HPNV NG +
Sbjct: 67 VAFRTKVFHPNVNSNGSI 84
>At1g16890.2 68414.m02044 ubiquitin-conjugating enzyme, putative
nearly identical to ubiquitin-conjugating enzyme E2
[Catharanthus roseus] GI:5381319; contains Pfam profile
PF00179: Ubiquitin-conjugating enzyme
Length = 153
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/16 (56%), Positives = 14/16 (87%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENG 643
+RFLTK++HPN+ + G
Sbjct: 71 VRFLTKIYHPNIDKLG 86
>At1g16890.1 68414.m02043 ubiquitin-conjugating enzyme, putative
nearly identical to ubiquitin-conjugating enzyme E2
[Catharanthus roseus] GI:5381319; contains Pfam profile
PF00179: Ubiquitin-conjugating enzyme
Length = 120
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/16 (56%), Positives = 14/16 (87%)
Frame = +2
Query: 596 IRFLTKVWHPNVYENG 643
+RFLTK++HPN+ + G
Sbjct: 38 VRFLTKIYHPNIDKLG 53
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,427,166
Number of Sequences: 28952
Number of extensions: 320362
Number of successful extensions: 787
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 735
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 785
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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