BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060966.seq (685 letters) Database: tribolium 336 sequences; 122,585 total letters Searching.......................................................done Score E Sequences producing significant alignments: (bits) Value AY887136-1|AAW78361.1| 580|Tribolium castaneum vasa RNA helicas... 60 2e-11 >AY887136-1|AAW78361.1| 580|Tribolium castaneum vasa RNA helicase protein. Length = 580 Score = 59.7 bits (138), Expect = 2e-11 Identities = 29/83 (34%), Positives = 44/83 (53%), Gaps = 1/83 (1%) Frame = +1 Query: 247 KQPPPIRRGDG-PIALVLAPTRELAQQIQQVAADFGHTSYVRNTCVFGGAPKREQARDLE 423 K PP P+ ++++PTRELA QI F + S V+ ++GG Q + Sbjct: 224 KNPPNTENNCAQPVVVIMSPTRELAIQIADQGKKFAYNSTVKVAVIYGGTSTNHQRGRIL 283 Query: 424 RGVEIVIATPGRLIDFLEKGTTN 492 G I++ATPGRL DF+ +G + Sbjct: 284 GGCHILVATPGRLKDFVNRGNVS 306 Score = 44.0 bits (99), Expect = 1e-06 Identities = 24/54 (44%), Positives = 35/54 (64%), Gaps = 4/54 (7%) Frame = +3 Query: 468 FLGKGHNQLTAVHILVLDEADRMLDMGFEPQIRKII-EQIRP---DRQTLMWSA 617 F+ +G+ ++ VLDEADRMLDMGF + +++ Q P +RQTLM+SA Sbjct: 299 FVNRGNVSFNSLKYFVLDEADRMLDMGFLGDVEEMLSHQSMPATGERQTLMFSA 352 Score = 43.2 bits (97), Expect = 2e-06 Identities = 20/55 (36%), Positives = 32/55 (58%) Frame = +3 Query: 21 EVTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGKNLV 185 EV V+G + PI FE + ++ + VK GY +PT IQ P+ +SG++L+ Sbjct: 145 EVKVTGNDAPPPITSFETSGLRPHLLENVKKSGYTKPTAIQKYAIPVILSGRDLM 199 Score = 27.5 bits (58), Expect = 0.11 Identities = 12/17 (70%), Positives = 15/17 (88%) Frame = +2 Query: 194 QTGSGKTLAYILPAIVH 244 QTGSGKT A++LP I+H Sbjct: 203 QTGSGKTAAFMLP-IIH 218 Database: tribolium Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 122,585 Number of sequences in database: 336 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 173,347 Number of Sequences: 336 Number of extensions: 4102 Number of successful extensions: 6 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 6 length of database: 122,585 effective HSP length: 55 effective length of database: 104,105 effective search space used: 17906060 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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