Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060964.seq
(684 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 124 1e-29
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 77 2e-15
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 74 2e-14
SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 72 9e-14
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 65 8e-12
SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit... 64 2e-11
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 61 2e-10
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 52 8e-08
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 32 0.067
SPCC16A11.17 |cdc21|mcm4, SPCC24B10.01|MCM complex subunit Cdc21... 27 1.9
SPAC144.03 |ade2|min10, min3|adenylosuccinate synthetase Ade2|Sc... 27 3.3
SPBC530.02 |||membrane transporter|Schizosaccharomyces pombe|chr... 27 3.3
SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase Snf21|... 26 4.4
SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase Snf22... 26 5.8
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 25 7.7
SPBC16H5.11c |skb1|rmt5|type II protein arginine N-methyltransfe... 25 7.7
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 25 7.7
SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomy... 25 7.7
>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
Cct7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 558
Score = 124 bits (299), Expect = 1e-29
Identities = 66/136 (48%), Positives = 85/136 (62%)
Frame = +3
Query: 246 PCRKTLVDIAKSQDAEVGDGTTSVVILAGEILKRLKPFVEEGVHPRVLIRAVRTASRLAI 425
P KTLVDIA++QDAEVGDGTTSVV+ AGE+L+ + FVE+GV ++IR R A++LA+
Sbjct: 78 PAAKTLVDIARAQDAEVGDGTTSVVVFAGELLREARTFVEDGVSSHLIIRGYRKAAQLAV 137
Query: 426 EKIKEQAVKIDNKSPEEQRDLLLKCASTRCHRN*FINRRITSXXXXXXXXXXXXXHFLPL 605
KIKE A+ +D + RDLL KCAST + + L
Sbjct: 138 NKIKEIAIHLDLSDEGKLRDLLTKCASTAMNSKLIRSNSTFFTKMVVDAVLTLDQEDLNE 197
Query: 606 DMIGIKKVPGGALEDS 653
+MIGIKKVPGGA+EDS
Sbjct: 198 NMIGIKKVPGGAMEDS 213
Score = 111 bits (268), Expect = 7e-26
Identities = 52/77 (67%), Positives = 63/77 (81%)
Frame = +1
Query: 34 QPQILLLREGTDQTQGKPQLVSNINACQLVVDAVRTTLGPRGMDKLIVDHNGKAVISNDG 213
Q +++L+EGTD +QG+ QL+SNINAC V D +RTTLGP G DKL+VD G+ VISNDG
Sbjct: 7 QIPVIVLKEGTDDSQGRGQLLSNINACVAVQDTIRTTLGPLGADKLMVDDRGEVVISNDG 66
Query: 214 ATIMKLLDIVHPAARLL 264
ATIMKLLDIVHPAA+ L
Sbjct: 67 ATIMKLLDIVHPAAKTL 83
>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
Cct4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 527
Score = 77.0 bits (181), Expect = 2e-15
Identities = 37/72 (51%), Positives = 53/72 (73%), Gaps = 2/72 (2%)
Frame = +1
Query: 70 QTQGKPQLV--SNINACQLVVDAVRTTLGPRGMDKLIVDHNGKAVISNDGATIMKLLDIV 243
Q + KPQ V SNI A + V DA+RT+LGP+GMDK+I G+ +++NDGATI+K L ++
Sbjct: 12 QDREKPQEVRLSNIMAARSVADAIRTSLGPKGMDKMIQTGKGEVILTNDGATILKHLSVL 71
Query: 244 HPAARLLWTLQS 279
HPAA++L L +
Sbjct: 72 HPAAKMLVDLSA 83
Score = 69.7 bits (163), Expect = 4e-13
Identities = 35/88 (39%), Positives = 56/88 (63%)
Frame = +3
Query: 246 PCRKTLVDIAKSQDAEVGDGTTSVVILAGEILKRLKPFVEEGVHPRVLIRAVRTASRLAI 425
P K LVD++ +QD E GDGTTSVVILAG +L + +++G+HP V+ + + A+ +
Sbjct: 73 PAAKMLVDLSAAQDVEAGDGTTSVVILAGSMLACAEKLLKKGIHPTVIAESFQRAAGFTV 132
Query: 426 EKIKEQAVKIDNKSPEEQRDLLLKCAST 509
+ +KE A+ I+ R+ LL+ A+T
Sbjct: 133 DCMKENALAIE----LSDRESLLRAATT 156
>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
Cct3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 528
Score = 74.1 bits (174), Expect = 2e-14
Identities = 35/89 (39%), Positives = 55/89 (61%)
Frame = +3
Query: 246 PCRKTLVDIAKSQDAEVGDGTTSVVILAGEILKRLKPFVEEGVHPRVLIRAVRTASRLAI 425
P K+++++A++QD EVGDGTTSV+ILAGEIL P ++ +HP V+IR+ + A A+
Sbjct: 73 PAAKSMIELARTQDEEVGDGTTSVIILAGEILAAASPLLDRKIHPVVMIRSFKQALEDAL 132
Query: 426 EKIKEQAVKIDNKSPEEQRDLLLKCASTR 512
I E + ++ E L+ C T+
Sbjct: 133 SIIDEITLPVNVDDNAEMFRLIRTCIGTK 161
Score = 64.1 bits (149), Expect = 2e-11
Identities = 27/76 (35%), Positives = 47/76 (61%)
Frame = +1
Query: 31 MQPQILLLREGTDQTQGKPQLVSNINACQLVVDAVRTTLGPRGMDKLIVDHNGKAVISND 210
MQ + ++ ++ G +SNI A + V D +RT LGPR M K+++D G +++ND
Sbjct: 1 MQSPVFVMNTNGNRQVGHKAQMSNIQAAKAVADVIRTCLGPRAMLKMLLDPVGSVLLTND 60
Query: 211 GATIMKLLDIVHPAAR 258
G I++ +++ HPAA+
Sbjct: 61 GHAILREIEVAHPAAK 76
>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
Cct2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 71.7 bits (168), Expect = 9e-14
Identities = 49/135 (36%), Positives = 70/135 (51%), Gaps = 1/135 (0%)
Frame = +3
Query: 255 KTLVDIAKSQDAEVGDGTTSVVILAGEILKRLKPFVEEGVHPRVLIRAVRTASRLAIEKI 434
K LV+I+K QD EVGDGTTSV + A E+L++ + V +HP+V+I R A++ AI+ +
Sbjct: 78 KVLVNISKVQDDEVGDGTTSVCVFAAELLRQAEIMVNAKIHPQVIIDGYRIATKTAIDAL 137
Query: 435 KEQAVKIDNKS-PEEQRDLLLKCASTRCHRN*FINRRITSXXXXXXXXXXXXXHFLPLDM 611
+ A IDN S P + R L A T +++ LD
Sbjct: 138 R--ASSIDNSSDPAKFRSDLENIARTTLSSK-ILSQNKNHFAQLAVDAVLRLKGSTNLDN 194
Query: 612 IGIKKVPGGALEDSF 656
I I K+ GG L+DSF
Sbjct: 195 IQIIKILGGKLDDSF 209
Score = 53.6 bits (123), Expect = 3e-08
Identities = 27/80 (33%), Positives = 50/80 (62%), Gaps = 1/80 (1%)
Frame = +1
Query: 49 LLREGTDQTQGKPQLVSNINACQLVVDAVRTTLGPRGMDKLIVDH-NGKAVISNDGATIM 225
+ E Q +G+ +S+ V D V++TLGP+GMDK++ + +G V++NDGATI+
Sbjct: 8 IFNESGIQERGENARLSSFVGAIAVGDLVKSTLGPKGMDKILQSNSSGDIVVTNDGATIL 67
Query: 226 KLLDIVHPAARLLWTLQSLK 285
K + + + AA++L + ++
Sbjct: 68 KSIALDNAAAKVLVNISKVQ 87
>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
Cct1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 65.3 bits (152), Expect = 8e-12
Identities = 30/61 (49%), Positives = 41/61 (67%)
Frame = +3
Query: 246 PCRKTLVDIAKSQDAEVGDGTTSVVILAGEILKRLKPFVEEGVHPRVLIRAVRTASRLAI 425
P K LV++A+ QD EVGDGTTSVVI+A E+L+R V+ +HP +I R A R A+
Sbjct: 75 PAGKVLVELAQQQDKEVGDGTTSVVIIAAELLRRANELVKNKIHPTTIITGYRLAIREAV 134
Query: 426 E 428
+
Sbjct: 135 K 135
Score = 62.9 bits (146), Expect = 4e-11
Identities = 26/66 (39%), Positives = 44/66 (66%)
Frame = +1
Query: 67 DQTQGKPQLVSNINACQLVVDAVRTTLGPRGMDKLIVDHNGKAVISNDGATIMKLLDIVH 246
++ G+ N+ A + + V+++LGP G+DK++VD G ++NDGATI+ LLD+ H
Sbjct: 15 EKISGEDVRNQNVLATTAIANVVKSSLGPVGLDKMLVDDIGDVTVTNDGATILSLLDVEH 74
Query: 247 PAARLL 264
PA ++L
Sbjct: 75 PAGKVL 80
Score = 35.1 bits (77), Expect = 0.010
Identities = 18/37 (48%), Positives = 23/37 (62%)
Frame = +2
Query: 479 KRSPIEVCINAMSSKLIHQQKDHFSKIVVDAVLSLDT 589
K S I V +MSSK+I D FS + VDA+LS+ T
Sbjct: 150 KESLINVAKTSMSSKIIGNDSDFFSTMAVDAMLSVKT 186
>SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit
Cct8 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 546
Score = 63.7 bits (148), Expect = 2e-11
Identities = 29/73 (39%), Positives = 50/73 (68%), Gaps = 1/73 (1%)
Frame = +1
Query: 49 LLREGTDQTQG-KPQLVSNINACQLVVDAVRTTLGPRGMDKLIVDHNGKAVISNDGATIM 225
L REG QG + ++ N NA + + + RT+LGP G +K++V+H + ++ND ATI+
Sbjct: 13 LFREGYRIMQGVEDAVIRNCNAIRELSEITRTSLGPNGKNKIVVNHLQQTFLTNDAATII 72
Query: 226 KLLDIVHPAARLL 264
+ L+++HPAA+L+
Sbjct: 73 RELEVIHPAAKLV 85
Score = 42.7 bits (96), Expect = 5e-05
Identities = 26/95 (27%), Positives = 48/95 (50%), Gaps = 2/95 (2%)
Frame = +3
Query: 246 PCRKTLVDIAKSQDAEVGDGTTSVVILAGEILKRLKPFVEEGVHPRVLIRAVRTASRLAI 425
P K +VD + Q+ E+GD VV+ GE+L + + + G+ P + + A +
Sbjct: 80 PAAKLVVDATQQQENELGDAANFVVVFTGELLAKAENMIRMGLTPLEIAKGYEMALSHTM 139
Query: 426 EKIKE-QAVKIDN-KSPEEQRDLLLKCASTRCHRN 524
E ++E A KI+ +S +E + C S++ + N
Sbjct: 140 EVLEEICADKIETVESEKELIKAIRTCISSKQYGN 174
>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
Cct5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 546
Score = 60.9 bits (141), Expect = 2e-10
Identities = 27/70 (38%), Positives = 47/70 (67%)
Frame = +1
Query: 55 REGTDQTQGKPQLVSNINACQLVVDAVRTTLGPRGMDKLIVDHNGKAVISNDGATIMKLL 234
+E + G + S+I A + V + VRT+LGPRG+DK+++ +G+ ++NDGATI+ +
Sbjct: 24 QEKKRRLHGIDAVKSHILATKTVANIVRTSLGPRGLDKILISPDGEITVTNDGATILDQM 83
Query: 235 DIVHPAARLL 264
++ H A+LL
Sbjct: 84 EVEHQIAKLL 93
Score = 60.5 bits (140), Expect = 2e-10
Identities = 29/78 (37%), Positives = 50/78 (64%)
Frame = +3
Query: 255 KTLVDIAKSQDAEVGDGTTSVVILAGEILKRLKPFVEEGVHPRVLIRAVRTASRLAIEKI 434
K LV ++KSQD E+GDGTT VV+LAG +L++ + +++G+HP + A ++A++ +
Sbjct: 91 KLLVQLSKSQDDEIGDGTTGVVVLAGALLEQAEALIDKGIHPIRIADGYEKACQVAVKHL 150
Query: 435 KEQAVKIDNKSPEEQRDL 488
+ +D SPE +L
Sbjct: 151 DAISDVVD-FSPENTTNL 167
Score = 30.7 bits (66), Expect = 0.20
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = +2
Query: 509 AMSSKLIHQQKDHFSKIVVDAVLSL 583
++ SK++ + DHF+ I VDAVLS+
Sbjct: 174 SLGSKVVSKAHDHFANIAVDAVLSV 198
>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
Cct6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 52.0 bits (119), Expect = 8e-08
Identities = 30/90 (33%), Positives = 48/90 (53%)
Frame = +3
Query: 240 RSPCRKTLVDIAKSQDAEVGDGTTSVVILAGEILKRLKPFVEEGVHPRVLIRAVRTASRL 419
++P + A +QD GDGTTSV +L GE+LK+ + ++ EG+HP ++ A
Sbjct: 68 QNPTASCIAKAATAQDDATGDGTTSVCLLVGELLKQAELYIREGLHPSLISDGFNLAKNE 127
Query: 420 AIEKIKEQAVKIDNKSPEEQRDLLLKCAST 509
A+ + + K D E R++LL A T
Sbjct: 128 ALTFL--DSFKTDF---EVDREVLLNVAKT 152
Score = 40.3 bits (90), Expect = 3e-04
Identities = 21/69 (30%), Positives = 35/69 (50%)
Frame = +1
Query: 49 LLREGTDQTQGKPQLVSNINACQLVVDAVRTTLGPRGMDKLIVDHNGKAVISNDGATIMK 228
LL + Q L NI+A + D +++ LGP G K++VD G ++ DG ++
Sbjct: 4 LLNPKAESIQRAQALQVNISAAIGLQDVLKSNLGPTGTTKMLVDGAGAIKLTKDGKVLLT 63
Query: 229 LLDIVHPAA 255
+ I +P A
Sbjct: 64 EMQIQNPTA 72
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 32.3 bits (70), Expect = 0.067
Identities = 22/70 (31%), Positives = 32/70 (45%)
Frame = +3
Query: 267 DIAKSQDAEVGDGTTSVVILAGEILKRLKPFVEEGVHPRVLIRAVRTASRLAIEKIKEQA 446
D+A + GDGTT+ +L I V G +P L R ++ A +E + QA
Sbjct: 107 DVASKTNEVAGDGTTTATVLTRAIFSETVRNVAAGCNPMDLRRGIQLAVDNVVEFL--QA 164
Query: 447 VKIDNKSPEE 476
K D + EE
Sbjct: 165 NKRDITTSEE 174
Score = 30.3 bits (65), Expect = 0.27
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +1
Query: 130 AVRTTLGPRGMDKLIVDHNGKAVISNDGATIMKLLDI 240
AV TLGP+G + LI G I+ DG T+ + + +
Sbjct: 57 AVSVTLGPKGRNVLIDQPFGSPKITKDGVTVARSVSL 93
>SPCC16A11.17 |cdc21|mcm4, SPCC24B10.01|MCM complex subunit
Cdc21|Schizosaccharomyces pombe|chr 3|||Manual
Length = 911
Score = 27.5 bits (58), Expect = 1.9
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = -1
Query: 282 ETLQCPQESCGRVNDIQ*LHD 220
E ++CP+E CG N +Q +H+
Sbjct: 344 EPIKCPREVCGATNAMQLIHN 364
>SPAC144.03 |ade2|min10, min3|adenylosuccinate synthetase
Ade2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 434
Score = 26.6 bits (56), Expect = 3.3
Identities = 19/69 (27%), Positives = 34/69 (49%)
Frame = +3
Query: 252 RKTLVDIAKSQDAEVGDGTTSVVILAGEILKRLKPFVEEGVHPRVLIRAVRTASRLAIEK 431
RK + D+ K A D + +I E+ +RLKPFV + V + A+++ R+ +E
Sbjct: 175 RKNVADLQKRYGAFEYD-VEAELIRYKELAQRLKPFVIDAV--AFMYEALQSKKRILVEG 231
Query: 432 IKEQAVKID 458
+ +D
Sbjct: 232 ANALMLDLD 240
>SPBC530.02 |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 541
Score = 26.6 bits (56), Expect = 3.3
Identities = 21/78 (26%), Positives = 38/78 (48%), Gaps = 2/78 (2%)
Frame = -3
Query: 613 IMSRGRKWSVQRQDSINNNLREV--ILLLMNQFR*HRVDAHFNRRSLCSSGDLLSIFTAC 440
+++ GRK+ V + D I+NN V + +N+ +DA R+ + S G ++ C
Sbjct: 5 LLASGRKFGVVKSDCISNNGVNVKDVGNALNEGVVGAIDA--TRKMVVSLGTVMPEIQKC 62
Query: 439 SLIFSMASLDAVLTALIR 386
+ F +S D L+R
Sbjct: 63 GVTFEESSEDVSDFYLVR 80
>SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase
Snf21|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1199
Score = 26.2 bits (55), Expect = 4.4
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = +3
Query: 363 EEGVHPRVLIRAVRTASRLAIEKIKEQAVKIDNKSPEEQRDLLLK 497
E+ V +L RA +L I+ QA K DNKS E+R+ L+
Sbjct: 863 EKSVEENILARA---QYKLDIDGKVIQAGKFDNKSTPEEREAFLR 904
>SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase
Snf22|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1680
Score = 25.8 bits (54), Expect = 5.8
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +3
Query: 348 LKPFVEEGVHPRVLIRAVRTASRLAIEKIKEQAVKIDNKSPEEQRDLLLK 497
L+ E+ + +L RA +L ++ QA K DNKS E+R+ L+
Sbjct: 1309 LRLITEKSIEENILSRA---QYKLDLDGKVIQAGKFDNKSTPEEREAFLR 1355
>SPBC3E7.01 |fab1|ste12,
SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1932
Score = 25.4 bits (53), Expect = 7.7
Identities = 8/15 (53%), Positives = 13/15 (86%)
Frame = +3
Query: 612 IGIKKVPGGALEDSF 656
+ IKK+PGG+++D F
Sbjct: 525 VKIKKIPGGSIQDCF 539
>SPBC16H5.11c |skb1|rmt5|type II protein arginine
N-methyltransferase Skb1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 645
Score = 25.4 bits (53), Expect = 7.7
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = -3
Query: 628 TFLIPIMSRGRKWSVQRQDSINNNLREVILLLMNQFR*HRVDAHFNRRSLCS 473
+++ PIMS + WS R + N ++LMN F D F +SL S
Sbjct: 458 SYVTPIMSP-KLWSEARNMNDPNAFERQYVVLMNSFDFLAADDEFRFQSLWS 508
>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 995
Score = 25.4 bits (53), Expect = 7.7
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = -1
Query: 528 ISFDDIALMHTSIGDLFVPQATYYQFSQ 445
+SFD I +H SI DL+ Q +QFS+
Sbjct: 116 VSFDSIDRLHVSIQDLYGAQ---FQFSK 140
>SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 573
Score = 25.4 bits (53), Expect = 7.7
Identities = 7/15 (46%), Positives = 11/15 (73%)
Frame = +3
Query: 159 DGQADCRSQWKGGYI 203
DG+ +C S+WK Y+
Sbjct: 555 DGKVECNSKWKSWYV 569
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,906,945
Number of Sequences: 5004
Number of extensions: 58686
Number of successful extensions: 205
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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