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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060962.seq
         (728 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar...    79   5e-16
SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharo...    68   2e-12
SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosacchar...    37   0.003
SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||...    31   0.13 
SPAC589.04 |||metaxin 1|Schizosaccharomyces pombe|chr 1|||Manual       29   0.90 
SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|ch...    27   2.1  
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces...    27   3.6  
SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo...    26   4.8  
SPBC30D10.11 |gpi1||pig-Q|Schizosaccharomyces pombe|chr 2|||Manual     26   6.3  
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    26   6.3  
SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    26   6.3  
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ...    25   8.4  
SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex...    25   8.4  
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    25   8.4  

>SPAC23H4.09 |cdb4||curved DNA-binding protein
           Cdb4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 381

 Score = 79.4 bits (187), Expect = 5e-16
 Identities = 41/91 (45%), Positives = 50/91 (54%)
 Frame = +1

Query: 235 HLSLFAHASEPDYILKKGDLAKIDLGAHIDGFIAVVAHTVVVGESEVSGRAADVXXXXXX 414
           HLS      E +  LK GD+ KI LGAHIDGF ++VA T VV E  V+G AADV      
Sbjct: 91  HLSPLKSDPEANLALKSGDVVKILLGAHIDGFASLVATTTVVSEEPVTGPAADVIAAASA 150

Query: 415 XXXXXXXXXKPGTENYAVTEAIQKISAEYGC 507
                    KPG  N+ VT+ + KI+  YGC
Sbjct: 151 ALKAAQRTIKPGNTNWQVTDIVDKIATSYGC 181



 Score = 72.9 bits (171), Expect = 4e-14
 Identities = 34/88 (38%), Positives = 56/88 (63%)
 Frame = +2

Query: 2   VEKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKE 181
           V+ +++    V KYK+AG++   V+++V+  C P A   +IC  GD+L+ E   KV+ + 
Sbjct: 12  VDYSLSNPETVNKYKIAGEVSQNVIKKVVELCQPGAKIYDICVRGDELLNEAIKKVY-RT 70

Query: 182 KDSKKGIAFSTCVSVNNCICHFSPMRAN 265
           KD+ KGIAF T VS N+   H SP++++
Sbjct: 71  KDAYKGIAFPTAVSPNDMAAHLSPLKSD 98



 Score = 64.1 bits (149), Expect = 2e-11
 Identities = 28/52 (53%), Positives = 40/52 (76%)
 Frame = +3

Query: 510 PIEGMLSHQLKQFRIDGEKSIILNPSEAQRXEHEKATLEKYEVYAMDVLIST 665
           P+ GMLSHQ ++  IDG+K +ILNPS++QR E +  T E+ EVY +D+L+ST
Sbjct: 183 PVAGMLSHQQEREVIDGKKQVILNPSDSQRSEMDTFTFEEGEVYGVDILVST 234


>SPBC23E6.05 |arx1||ribosomal export complex Arx1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 417

 Score = 67.7 bits (158), Expect = 2e-12
 Identities = 30/73 (41%), Positives = 49/73 (67%)
 Frame = +2

Query: 35  TKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKEKDSKKGIAFST 214
           +KY+ AG +V++   QV ++CVP AS REI  +GD L+ E  + ++K ++  +KGIA  T
Sbjct: 19  SKYRDAGALVSKAFHQVASRCVPGASTREISSYGDNLLHEYKSSIYKSQR-FEKGIAEPT 77

Query: 215 CVSVNNCICHFSP 253
            + VNNC  +++P
Sbjct: 78  SICVNNCAYNYAP 90



 Score = 43.2 bits (97), Expect = 4e-05
 Identities = 21/66 (31%), Positives = 34/66 (51%)
 Frame = +1

Query: 163 QGFQKGERLQERHCIFNMRFCEQLHLSLFAHASEPDYILKKGDLAKIDLGAHIDGFIAVV 342
           Q F+KG       C+ N  +            ++  Y L+ GD+ KI +G H DG+ A++
Sbjct: 67  QRFEKGIAEPTSICVNNCAYNYAPGPESVIAGNDNSYHLQVGDVTKISMGLHFDGYTALI 126

Query: 343 AHTVVV 360
           +HT+VV
Sbjct: 127 SHTIVV 132


>SPBC14C8.03 |fma2||methionine aminopeptidase Fma2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 426

 Score = 36.7 bits (81), Expect = 0.003
 Identities = 15/48 (31%), Positives = 26/48 (54%)
 Frame = +2

Query: 149 LEETNKVFKKEKDSKKGIAFSTCVSVNNCICHFSPMRANRITF*KKEI 292
           +E T +   +E   K GI F T VS+N+C  H++P   +     +K++
Sbjct: 148 IENTTRALVEEDGLKSGIGFPTGVSLNHCAAHYTPNAGDTTILKEKDV 195



 Score = 29.5 bits (63), Expect = 0.51
 Identities = 12/27 (44%), Positives = 19/27 (70%)
 Frame = +1

Query: 274 ILKKGDLAKIDLGAHIDGFIAVVAHTV 354
           ILK+ D+ K+D+G H++G I   A T+
Sbjct: 189 ILKEKDVMKVDIGVHVNGRIVDSAFTM 215


>SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 117

 Score = 31.5 bits (68), Expect = 0.13
 Identities = 20/61 (32%), Positives = 28/61 (45%), Gaps = 3/61 (4%)
 Frame = -3

Query: 483 LNGFSDCIVLSTR---LQETKSRFTSMMSCQQNICSTSRDFTFTHNYCMSYNSYKSINVS 313
           L G S  I LST    L + K+ +  M +     C  S  +T + N C +Y +  SIN  
Sbjct: 12  LLGLSTAIPLSTEADALLDRKTIYFGMKAYDDVNCKGSTSYTISFNKCQAYPNINSINAL 71

Query: 312 T 310
           T
Sbjct: 72  T 72


>SPAC589.04 |||metaxin 1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 271

 Score = 28.7 bits (61), Expect = 0.90
 Identities = 15/37 (40%), Positives = 20/37 (54%)
 Frame = +1

Query: 208 FNMRFCEQLHLSLFAHASEPDYILKKGDLAKIDLGAH 318
           FN      L +SLFAHA   +++  K D  K+ LG H
Sbjct: 210 FNNESPSFLDVSLFAHAEIINHLPLKNDQLKVVLGTH 246


>SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 658

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 31/137 (22%), Positives = 59/137 (43%), Gaps = 14/137 (10%)
 Frame = -2

Query: 535 WCESI--PSIGYNHIQHLFSE---WLQ*LHSSQYQASGDEEPLH*HDELPTKHLQHVQRL 371
           W  S+  PS+ Y  + + FS     +   +SS +      +P   ++ L ++ LQ   +L
Sbjct: 368 WQHSVWRPSLAYLQVVYSFSMRALLVNRQYSSAFAFFWKIDPYIRNERLVSQMLQAASQL 427

Query: 370 HFHPQLLYELQQL----*IHQCEHQDLSWLNLLFSECN--PVRSHGRKV---TNAVVHRN 218
           H+H  +LY +        +    ++DL  +      CN   ++    K+   TN V+ ++
Sbjct: 428 HYHDLILYVINTYYSGNIMKNLGNKDLICITQSSPPCNISGIKPGSLKLSPTTNTVLAKS 487

Query: 217 AC*KCNAFLGVFLLFEN 167
            C   N  + +  L EN
Sbjct: 488 ICYWLNDAMALLFLLEN 504


>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 713

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 12/40 (30%), Positives = 19/40 (47%)
 Frame = -2

Query: 676 TASPVDIRTSIAYTSYFSSVAFSCSXRCASDGFSIILFSP 557
           TASP+ +RTS   ++Y +    + S  C S   +     P
Sbjct: 216 TASPIPVRTSSVSSTYLTQDREASSKNCLSKALAFSSIEP 255


>SPAC343.11c |msc1||multi-copy suppressor of Chk1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1588

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 13/32 (40%), Positives = 17/32 (53%)
 Frame = +3

Query: 96  AFQTHQQEKYVNLAINLYWRRLTRFSKRRKTP 191
           AF T +Q  Y N   NLY+   ++ S  R TP
Sbjct: 474 AFPTSRQNAYYNDPWNLYFIHFSKLSPLRFTP 505


>SPBC30D10.11 |gpi1||pig-Q|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 653

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 25/82 (30%), Positives = 40/82 (48%)
 Frame = -2

Query: 364 HPQLLYELQQL*IHQCEHQDLSWLNLLFSECNPVRSHGRKVTNAVVHRNAC*KCNAFLGV 185
           H Q+L  L++L +H    ++ SW   L S    + +   +V   + H N   K N++  V
Sbjct: 167 HQQIL--LKKLKLHFPRRKENSWKRSLRSGLIELLNQSFEV-RMLTHENNN-KKNSY--V 220

Query: 184 FLLFENLVSLLQYKFIAKFTYF 119
           F LF+ + S   Y F + F YF
Sbjct: 221 FRLFDRVSSSTFYFFNSLFAYF 242


>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 357

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 9/24 (37%), Positives = 14/24 (58%)
 Frame = -3

Query: 354 YCMSYNSYKSINVSTKIYLG*ISF 283
           YC    +YKS+ + T +Y+G   F
Sbjct: 144 YCRKVLTYKSLGIRTAVYVGAFCF 167


>SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 681

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 12/35 (34%), Positives = 19/35 (54%)
 Frame = -2

Query: 145 KFIAKFTYFSC*CVWNAFCDDLLKDSVHYLSC*LV 41
           K + KF+   C  +++A C+D L  S H   C +V
Sbjct: 472 KCLNKFSQEDCQFIFDAICEDPLDVSTHRHGCCVV 506


>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1044

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 11/27 (40%), Positives = 16/27 (59%)
 Frame = +2

Query: 107 ASAREICEFGDKLVLEETNKVFKKEKD 187
           ++ +E   FG+KL  E T K+   EKD
Sbjct: 865 SNLQEKINFGNKLAKEHTEKISSLEKD 891


>SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex
           subunit Tim13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 95

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 10/33 (30%), Positives = 21/33 (63%)
 Frame = +2

Query: 8   KTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPD 106
           K I ++L V +   AG++++++ E    KC+P+
Sbjct: 23  KQIRQELAVAQ---AGELISKINENCFDKCIPE 52


>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 486

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 12/32 (37%), Positives = 17/32 (53%)
 Frame = +1

Query: 262 EPDYILKKGDLAKIDLGAHIDGFIAVVAHTVV 357
           EP+Y+L  G+L+  +L   ID F      T V
Sbjct: 382 EPEYLLMYGNLSNEELVERIDAFYMNARRTYV 413


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,954,795
Number of Sequences: 5004
Number of extensions: 59028
Number of successful extensions: 208
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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