BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060962.seq (728 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar... 79 5e-16 SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharo... 68 2e-12 SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosacchar... 37 0.003 SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 31 0.13 SPAC589.04 |||metaxin 1|Schizosaccharomyces pombe|chr 1|||Manual 29 0.90 SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|ch... 27 2.1 SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 3.6 SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 26 4.8 SPBC30D10.11 |gpi1||pig-Q|Schizosaccharomyces pombe|chr 2|||Manual 26 6.3 SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 6.3 SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 6.3 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 8.4 SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex... 25 8.4 SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 8.4 >SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosaccharomyces pombe|chr 1|||Manual Length = 381 Score = 79.4 bits (187), Expect = 5e-16 Identities = 41/91 (45%), Positives = 50/91 (54%) Frame = +1 Query: 235 HLSLFAHASEPDYILKKGDLAKIDLGAHIDGFIAVVAHTVVVGESEVSGRAADVXXXXXX 414 HLS E + LK GD+ KI LGAHIDGF ++VA T VV E V+G AADV Sbjct: 91 HLSPLKSDPEANLALKSGDVVKILLGAHIDGFASLVATTTVVSEEPVTGPAADVIAAASA 150 Query: 415 XXXXXXXXXKPGTENYAVTEAIQKISAEYGC 507 KPG N+ VT+ + KI+ YGC Sbjct: 151 ALKAAQRTIKPGNTNWQVTDIVDKIATSYGC 181 Score = 72.9 bits (171), Expect = 4e-14 Identities = 34/88 (38%), Positives = 56/88 (63%) Frame = +2 Query: 2 VEKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKE 181 V+ +++ V KYK+AG++ V+++V+ C P A +IC GD+L+ E KV+ + Sbjct: 12 VDYSLSNPETVNKYKIAGEVSQNVIKKVVELCQPGAKIYDICVRGDELLNEAIKKVY-RT 70 Query: 182 KDSKKGIAFSTCVSVNNCICHFSPMRAN 265 KD+ KGIAF T VS N+ H SP++++ Sbjct: 71 KDAYKGIAFPTAVSPNDMAAHLSPLKSD 98 Score = 64.1 bits (149), Expect = 2e-11 Identities = 28/52 (53%), Positives = 40/52 (76%) Frame = +3 Query: 510 PIEGMLSHQLKQFRIDGEKSIILNPSEAQRXEHEKATLEKYEVYAMDVLIST 665 P+ GMLSHQ ++ IDG+K +ILNPS++QR E + T E+ EVY +D+L+ST Sbjct: 183 PVAGMLSHQQEREVIDGKKQVILNPSDSQRSEMDTFTFEEGEVYGVDILVST 234 >SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 417 Score = 67.7 bits (158), Expect = 2e-12 Identities = 30/73 (41%), Positives = 49/73 (67%) Frame = +2 Query: 35 TKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKEKDSKKGIAFST 214 +KY+ AG +V++ QV ++CVP AS REI +GD L+ E + ++K ++ +KGIA T Sbjct: 19 SKYRDAGALVSKAFHQVASRCVPGASTREISSYGDNLLHEYKSSIYKSQR-FEKGIAEPT 77 Query: 215 CVSVNNCICHFSP 253 + VNNC +++P Sbjct: 78 SICVNNCAYNYAP 90 Score = 43.2 bits (97), Expect = 4e-05 Identities = 21/66 (31%), Positives = 34/66 (51%) Frame = +1 Query: 163 QGFQKGERLQERHCIFNMRFCEQLHLSLFAHASEPDYILKKGDLAKIDLGAHIDGFIAVV 342 Q F+KG C+ N + ++ Y L+ GD+ KI +G H DG+ A++ Sbjct: 67 QRFEKGIAEPTSICVNNCAYNYAPGPESVIAGNDNSYHLQVGDVTKISMGLHFDGYTALI 126 Query: 343 AHTVVV 360 +HT+VV Sbjct: 127 SHTIVV 132 >SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 36.7 bits (81), Expect = 0.003 Identities = 15/48 (31%), Positives = 26/48 (54%) Frame = +2 Query: 149 LEETNKVFKKEKDSKKGIAFSTCVSVNNCICHFSPMRANRITF*KKEI 292 +E T + +E K GI F T VS+N+C H++P + +K++ Sbjct: 148 IENTTRALVEEDGLKSGIGFPTGVSLNHCAAHYTPNAGDTTILKEKDV 195 Score = 29.5 bits (63), Expect = 0.51 Identities = 12/27 (44%), Positives = 19/27 (70%) Frame = +1 Query: 274 ILKKGDLAKIDLGAHIDGFIAVVAHTV 354 ILK+ D+ K+D+G H++G I A T+ Sbjct: 189 ILKEKDVMKVDIGVHVNGRIVDSAFTM 215 >SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 31.5 bits (68), Expect = 0.13 Identities = 20/61 (32%), Positives = 28/61 (45%), Gaps = 3/61 (4%) Frame = -3 Query: 483 LNGFSDCIVLSTR---LQETKSRFTSMMSCQQNICSTSRDFTFTHNYCMSYNSYKSINVS 313 L G S I LST L + K+ + M + C S +T + N C +Y + SIN Sbjct: 12 LLGLSTAIPLSTEADALLDRKTIYFGMKAYDDVNCKGSTSYTISFNKCQAYPNINSINAL 71 Query: 312 T 310 T Sbjct: 72 T 72 >SPAC589.04 |||metaxin 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 271 Score = 28.7 bits (61), Expect = 0.90 Identities = 15/37 (40%), Positives = 20/37 (54%) Frame = +1 Query: 208 FNMRFCEQLHLSLFAHASEPDYILKKGDLAKIDLGAH 318 FN L +SLFAHA +++ K D K+ LG H Sbjct: 210 FNNESPSFLDVSLFAHAEIINHLPLKNDQLKVVLGTH 246 >SPBC16A3.03c |lyn1||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 658 Score = 27.5 bits (58), Expect = 2.1 Identities = 31/137 (22%), Positives = 59/137 (43%), Gaps = 14/137 (10%) Frame = -2 Query: 535 WCESI--PSIGYNHIQHLFSE---WLQ*LHSSQYQASGDEEPLH*HDELPTKHLQHVQRL 371 W S+ PS+ Y + + FS + +SS + +P ++ L ++ LQ +L Sbjct: 368 WQHSVWRPSLAYLQVVYSFSMRALLVNRQYSSAFAFFWKIDPYIRNERLVSQMLQAASQL 427 Query: 370 HFHPQLLYELQQL----*IHQCEHQDLSWLNLLFSECN--PVRSHGRKV---TNAVVHRN 218 H+H +LY + + ++DL + CN ++ K+ TN V+ ++ Sbjct: 428 HYHDLILYVINTYYSGNIMKNLGNKDLICITQSSPPCNISGIKPGSLKLSPTTNTVLAKS 487 Query: 217 AC*KCNAFLGVFLLFEN 167 C N + + L EN Sbjct: 488 ICYWLNDAMALLFLLEN 504 >SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 26.6 bits (56), Expect = 3.6 Identities = 12/40 (30%), Positives = 19/40 (47%) Frame = -2 Query: 676 TASPVDIRTSIAYTSYFSSVAFSCSXRCASDGFSIILFSP 557 TASP+ +RTS ++Y + + S C S + P Sbjct: 216 TASPIPVRTSSVSSTYLTQDREASSKNCLSKALAFSSIEP 255 >SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1588 Score = 26.2 bits (55), Expect = 4.8 Identities = 13/32 (40%), Positives = 17/32 (53%) Frame = +3 Query: 96 AFQTHQQEKYVNLAINLYWRRLTRFSKRRKTP 191 AF T +Q Y N NLY+ ++ S R TP Sbjct: 474 AFPTSRQNAYYNDPWNLYFIHFSKLSPLRFTP 505 >SPBC30D10.11 |gpi1||pig-Q|Schizosaccharomyces pombe|chr 2|||Manual Length = 653 Score = 25.8 bits (54), Expect = 6.3 Identities = 25/82 (30%), Positives = 40/82 (48%) Frame = -2 Query: 364 HPQLLYELQQL*IHQCEHQDLSWLNLLFSECNPVRSHGRKVTNAVVHRNAC*KCNAFLGV 185 H Q+L L++L +H ++ SW L S + + +V + H N K N++ V Sbjct: 167 HQQIL--LKKLKLHFPRRKENSWKRSLRSGLIELLNQSFEV-RMLTHENNN-KKNSY--V 220 Query: 184 FLLFENLVSLLQYKFIAKFTYF 119 F LF+ + S Y F + F YF Sbjct: 221 FRLFDRVSSSTFYFFNSLFAYF 242 >SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 357 Score = 25.8 bits (54), Expect = 6.3 Identities = 9/24 (37%), Positives = 14/24 (58%) Frame = -3 Query: 354 YCMSYNSYKSINVSTKIYLG*ISF 283 YC +YKS+ + T +Y+G F Sbjct: 144 YCRKVLTYKSLGIRTAVYVGAFCF 167 >SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 681 Score = 25.8 bits (54), Expect = 6.3 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = -2 Query: 145 KFIAKFTYFSC*CVWNAFCDDLLKDSVHYLSC*LV 41 K + KF+ C +++A C+D L S H C +V Sbjct: 472 KCLNKFSQEDCQFIFDAICEDPLDVSTHRHGCCVV 506 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 25.4 bits (53), Expect = 8.4 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +2 Query: 107 ASAREICEFGDKLVLEETNKVFKKEKD 187 ++ +E FG+KL E T K+ EKD Sbjct: 865 SNLQEKINFGNKLAKEHTEKISSLEKD 891 >SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex subunit Tim13|Schizosaccharomyces pombe|chr 1|||Manual Length = 95 Score = 25.4 bits (53), Expect = 8.4 Identities = 10/33 (30%), Positives = 21/33 (63%) Frame = +2 Query: 8 KTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPD 106 K I ++L V + AG++++++ E KC+P+ Sbjct: 23 KQIRQELAVAQ---AGELISKINENCFDKCIPE 52 >SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 486 Score = 25.4 bits (53), Expect = 8.4 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = +1 Query: 262 EPDYILKKGDLAKIDLGAHIDGFIAVVAHTVV 357 EP+Y+L G+L+ +L ID F T V Sbjct: 382 EPEYLLMYGNLSNEELVERIDAFYMNARRTYV 413 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,954,795 Number of Sequences: 5004 Number of extensions: 59028 Number of successful extensions: 208 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 195 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 206 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 343230174 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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