Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060962.seq
(728 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51800.2 68416.m05681 metallopeptidase M24 family protein sim... 87 9e-18
At3g51800.1 68416.m05680 metallopeptidase M24 family protein sim... 87 9e-18
At3g25740.1 68416.m03205 metallopeptidase M24 family protein sim... 33 0.26
At3g59990.2 68416.m06698 methionyl aminopeptidase, putative / me... 30 1.8
At3g59990.1 68416.m06697 methionyl aminopeptidase, putative / me... 30 1.8
At2g44180.1 68415.m05496 methionyl aminopeptidase, putative / me... 30 1.8
At4g37040.1 68417.m05246 metallopeptidase M24 family protein sim... 29 3.2
At1g09300.1 68414.m01041 metallopeptidase M24 family protein sim... 29 3.2
At3g04670.1 68416.m00500 WRKY family transcription factor simila... 29 4.2
At1g13270.2 68414.m01540 metallopeptidase M24 family protein sim... 28 5.5
At1g13270.1 68414.m01541 metallopeptidase M24 family protein sim... 28 5.5
At5g28650.1 68418.m03508 WRKY family transcription factor DNA-bi... 28 7.3
At4g12360.1 68417.m01954 protease inhibitor/seed storage/lipid t... 27 9.6
At3g06350.1 68416.m00733 dehydroquinate dehydratase, putative / ... 27 9.6
>At3g51800.2 68416.m05681 metallopeptidase M24 family protein
similar to SP|P50580 Proliferation-associated protein
2G4 {Mus musculus}; contains Pfam profile PF00557:
metallopeptidase family M24
Length = 401
Score = 87.4 bits (207), Expect = 9e-18
Identities = 39/88 (44%), Positives = 59/88 (67%), Gaps = 1/88 (1%)
Frame = +2
Query: 5 EKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKEK 184
E ++ VVTKYK A +IVN+ L+ V+A+C P A +ICE GD + E+T ++K K
Sbjct: 11 ELSLTSPEVVTKYKSAAEIVNKALQVVLAECKPKAKIVDICEKGDSFIKEQTASMYKNSK 70
Query: 185 DS-KKGIAFSTCVSVNNCICHFSPMRAN 265
++G+AF TC+SVNN + HFSP+ ++
Sbjct: 71 KKIERGVAFPTCISVNNTVGHFSPLASD 98
Score = 82.6 bits (195), Expect = 2e-16
Identities = 42/96 (43%), Positives = 57/96 (59%), Gaps = 1/96 (1%)
Frame = +1
Query: 247 FAHASEPDYILKKGDLAKIDLGAHIDGFIAVVAHTVVVGESEVSGRAADVXXXXXXXXXX 426
F+ + + +L+ GD+ KID+G HIDGFIA+V HT V+ E +SGR ADV
Sbjct: 92 FSPLASDESVLEDGDMVKIDMGCHIDGFIALVGHTHVLQEGPLSGRKADVIAAANTAADV 151
Query: 427 XXXXXKPGTENYAVTEAIQKISAEYGCSQLR-VCSH 531
+PG +N VTEAIQK++A Y C + V SH
Sbjct: 152 ALRLVRPGKKNTDVTEAIQKVAAAYDCKIVEGVLSH 187
Score = 49.2 bits (112), Expect = 3e-06
Identities = 27/68 (39%), Positives = 41/68 (60%)
Frame = +3
Query: 513 IEGMLSHQLKQFRIDGEKSIILNPSEAQRXEHEKATLEKYEVYAMDVLISTGEAVGREMG 692
+EG+LSHQLKQ IDG K ++L+ S + E E+ EVYA+D++ STG+ + +
Sbjct: 181 VEGVLSHQLKQHVIDGNK-VVLSVSSPETTVDE-VEFEENEVYAIDIVASTGDGKPKLLD 238
Query: 693 HQMYNIQK 716
+ I K
Sbjct: 239 EKQTTIYK 246
>At3g51800.1 68416.m05680 metallopeptidase M24 family protein
similar to SP|P50580 Proliferation-associated protein
2G4 {Mus musculus}; contains Pfam profile PF00557:
metallopeptidase family M24
Length = 392
Score = 87.4 bits (207), Expect = 9e-18
Identities = 39/88 (44%), Positives = 59/88 (67%), Gaps = 1/88 (1%)
Frame = +2
Query: 5 EKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKEK 184
E ++ VVTKYK A +IVN+ L+ V+A+C P A +ICE GD + E+T ++K K
Sbjct: 11 ELSLTSPEVVTKYKSAAEIVNKALQVVLAECKPKAKIVDICEKGDSFIKEQTASMYKNSK 70
Query: 185 DS-KKGIAFSTCVSVNNCICHFSPMRAN 265
++G+AF TC+SVNN + HFSP+ ++
Sbjct: 71 KKIERGVAFPTCISVNNTVGHFSPLASD 98
Score = 82.6 bits (195), Expect = 2e-16
Identities = 42/96 (43%), Positives = 57/96 (59%), Gaps = 1/96 (1%)
Frame = +1
Query: 247 FAHASEPDYILKKGDLAKIDLGAHIDGFIAVVAHTVVVGESEVSGRAADVXXXXXXXXXX 426
F+ + + +L+ GD+ KID+G HIDGFIA+V HT V+ E +SGR ADV
Sbjct: 92 FSPLASDESVLEDGDMVKIDMGCHIDGFIALVGHTHVLQEGPLSGRKADVIAAANTAADV 151
Query: 427 XXXXXKPGTENYAVTEAIQKISAEYGCSQLR-VCSH 531
+PG +N VTEAIQK++A Y C + V SH
Sbjct: 152 ALRLVRPGKKNTDVTEAIQKVAAAYDCKIVEGVLSH 187
Score = 49.2 bits (112), Expect = 3e-06
Identities = 27/68 (39%), Positives = 41/68 (60%)
Frame = +3
Query: 513 IEGMLSHQLKQFRIDGEKSIILNPSEAQRXEHEKATLEKYEVYAMDVLISTGEAVGREMG 692
+EG+LSHQLKQ IDG K ++L+ S + E E+ EVYA+D++ STG+ + +
Sbjct: 181 VEGVLSHQLKQHVIDGNK-VVLSVSSPETTVDE-VEFEENEVYAIDIVASTGDGKPKLLD 238
Query: 693 HQMYNIQK 716
+ I K
Sbjct: 239 EKQTTIYK 246
>At3g25740.1 68416.m03205 metallopeptidase M24 family protein
similar to SP|O33343 Methionine aminopeptidase (EC
3.4.11.18) (Peptidase M) {Mycobacterium tuberculosis};
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 344
Score = 32.7 bits (71), Expect = 0.26
Identities = 23/108 (21%), Positives = 43/108 (39%)
Frame = +1
Query: 208 FNMRFCEQLHLSLFAHASEPDYILKKGDLAKIDLGAHIDGFIAVVAHTVVVGESEVSGRA 387
F C ++ +F H L+ GD+ ID+ ++DG+ + T + G +V+G
Sbjct: 158 FPKSVCTSVNECMF-HGIPDSRPLQNGDIINIDVAVYLDGYHGDTSKTFLCG--DVNGSL 214
Query: 388 ADVXXXXXXXXXXXXXXXKPGTENYAVTEAIQKISAEYGCSQLRVCSH 531
+ K G + + I + +A+YG + R H
Sbjct: 215 KQLVKVTEECLEKGISVCKDGASFKQIGKIISEHAAKYGYNMERFIGH 262
>At3g59990.2 68416.m06698 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to Methionine aminopeptidase 2 (EC
3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo
sapiens} SP|P50579; contains Pfam profile PF00557:
metallopeptidase family M24; supporting cDNA
gi|11344921|gb|AF300880.1|AF300880
Length = 439
Score = 29.9 bits (64), Expect = 1.8
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +1
Query: 274 ILKKGDLAKIDLGAHIDGFIAVVAHTV 354
+L+ D+ K+D G HIDG I A TV
Sbjct: 202 VLQYDDVMKLDFGTHIDGHIIDCAFTV 228
>At3g59990.1 68416.m06697 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to Methionine aminopeptidase 2 (EC
3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo
sapiens} SP|P50579; contains Pfam profile PF00557:
metallopeptidase family M24; supporting cDNA
gi|11344921|gb|AF300880.1|AF300880
Length = 439
Score = 29.9 bits (64), Expect = 1.8
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +1
Query: 274 ILKKGDLAKIDLGAHIDGFIAVVAHTV 354
+L+ D+ K+D G HIDG I A TV
Sbjct: 202 VLQYDDVMKLDFGTHIDGHIIDCAFTV 228
>At2g44180.1 68415.m05496 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to SP|P50579 Methionine aminopeptidase
2 (EC 3.4.11.18) (MetAP 2) {Homo sapiens}; contains Pfam
profile PF00557: metallopeptidase family M24
Length = 441
Score = 29.9 bits (64), Expect = 1.8
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +1
Query: 274 ILKKGDLAKIDLGAHIDGFIAVVAHTV 354
+L+ D+ K+D G HIDG I A TV
Sbjct: 204 VLQYDDVMKLDFGTHIDGHIVDSAFTV 230
Score = 29.5 bits (63), Expect = 2.4
Identities = 19/75 (25%), Positives = 34/75 (45%)
Frame = +2
Query: 29 VVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKEKDSKKGIAF 208
+ + A ++ +V + + + P ++CE LE T + E + GIAF
Sbjct: 128 IYNSLRQAAEVHRQVRKYMRSILKPGMLMIDLCE-----TLENTVRKLISENGLQAGIAF 182
Query: 209 STCVSVNNCICHFSP 253
T S+NN H++P
Sbjct: 183 PTGCSLNNVAAHWTP 197
>At4g37040.1 68417.m05246 metallopeptidase M24 family protein
similar to SP|O33343 Methionine aminopeptidase (EC
3.4.11.18) (Peptidase M) {Mycobacterium tuberculosis};
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 350
Score = 29.1 bits (62), Expect = 3.2
Identities = 20/70 (28%), Positives = 29/70 (41%)
Frame = +2
Query: 44 KLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKEKDSKKGIAFSTCVS 223
+ +G + RV + P + EI E +++E N + G S C S
Sbjct: 117 RASGILAARVRDYAGTLVKPGVTTDEIDEAVHNMIIE--NGAYPSPL-GYGGFPKSVCTS 173
Query: 224 VNNCICHFSP 253
VN CICH P
Sbjct: 174 VNECICHGIP 183
>At1g09300.1 68414.m01041 metallopeptidase M24 family protein
similar to SP|P15034 Xaa-Pro aminopeptidase (EC
3.4.11.9) (X-Pro aminopeptidase) (Aminopeptidase P II)
(Aminoacylproline aminopeptidase) {Escherichia coli};
contains Pfam profiles PF00557: metallopeptidase family
M24, PF05195: Aminopeptidase P, N-terminal domain
Length = 493
Score = 29.1 bits (62), Expect = 3.2
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +1
Query: 253 HASEPDYILKKGDLAKIDLGAHIDGFIAVVAHT 351
H S D +K GDL +D+G + G+++ + T
Sbjct: 279 HYSRNDQRIKDGDLVLMDMGCELHGYVSDLTRT 311
>At3g04670.1 68416.m00500 WRKY family transcription factor similar
to elicitor response element binding protein WRKY3
isolog GB:AAB63078 [Arabidopsis thaliana]
Length = 330
Score = 28.7 bits (61), Expect = 4.2
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = -1
Query: 278 RM*SGSLAWAKSDKCSCSQKRMLKMQ 201
+M SGSL KC CS+KR L+++
Sbjct: 220 KMCSGSLKCGSRSKCHCSKKRKLRVK 245
>At1g13270.2 68414.m01540 metallopeptidase M24 family protein
similar to SP|Q01662 Methionine aminopeptidase 1
precursor (EC 3.4.11.18) {Saccharomyces cerevisiae};
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 283
Score = 28.3 bits (60), Expect = 5.5
Identities = 19/74 (25%), Positives = 29/74 (39%)
Frame = +2
Query: 32 VTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKEKDSKKGIAFS 211
+ K + A ++ RVL P + EI + +++E G S
Sbjct: 132 IAKMRAACELAARVLNYAGTLVKPSVTTNEIDKAVHDMIIEAGAY---PSPLGYGGFPKS 188
Query: 212 TCVSVNNCICHFSP 253
C SVN C+CH P
Sbjct: 189 VCTSVNECMCHGIP 202
>At1g13270.1 68414.m01541 metallopeptidase M24 family protein
similar to SP|Q01662 Methionine aminopeptidase 1
precursor (EC 3.4.11.18) {Saccharomyces cerevisiae};
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 369
Score = 28.3 bits (60), Expect = 5.5
Identities = 19/74 (25%), Positives = 29/74 (39%)
Frame = +2
Query: 32 VTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKEKDSKKGIAFS 211
+ K + A ++ RVL P + EI + +++E G S
Sbjct: 132 IAKMRAACELAARVLNYAGTLVKPSVTTNEIDKAVHDMIIEAGAY---PSPLGYGGFPKS 188
Query: 212 TCVSVNNCICHFSP 253
C SVN C+CH P
Sbjct: 189 VCTSVNECMCHGIP 202
>At5g28650.1 68418.m03508 WRKY family transcription factor
DNA-binding protein WRKY3, parsley, PIR:S72445
Length = 330
Score = 27.9 bits (59), Expect = 7.3
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -1
Query: 269 SGSLAWAKSDKCSCSQKRMLKMQ 201
SGSL KC CS+KR L+++
Sbjct: 223 SGSLKCGSKSKCHCSKKRKLRVK 245
>At4g12360.1 68417.m01954 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein contains Pfam
protease inhibitor/seed storage/LTP family domain
PF00234
Length = 161
Score = 27.5 bits (58), Expect = 9.6
Identities = 15/42 (35%), Positives = 20/42 (47%)
Frame = -3
Query: 174 LKTLLVSSNTSLSPNSHISLADASGTHFAMTCSRTRFTICPA 49
L TLLV++ T HI LA + + AM C+ PA
Sbjct: 11 LATLLVAATTVSGQGPHIPLAPSPSVNEAMNCAAGLAVCLPA 52
>At3g06350.1 68416.m00733 dehydroquinate dehydratase, putative /
shikimate dehydrogenase, putative similar to
dehydroquinate dehydratase/shikimate dehydrogenase
[Nicotiana tabacum][GI:535771], dehydroquinate
dehydratase/shikimate:NADP oxidoreductase [Lycopersicon
esculentum][GI:3169883]
Length = 603
Score = 27.5 bits (58), Expect = 9.6
Identities = 19/42 (45%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = -2
Query: 232 VVHRNAC*KCNAFLGVF--LLFENLVSLLQYKFIAKFTYFSC 113
+VH A K F GV+ LL +NLVS LQ + F FSC
Sbjct: 340 IVHNQAF-KSVDFNGVYVHLLVDNLVSFLQAYSSSDFAGFSC 380
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,203,220
Number of Sequences: 28952
Number of extensions: 298794
Number of successful extensions: 885
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 844
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 881
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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