BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060856.seq (671 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 86 2e-17 At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 83 2e-16 At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 81 5e-16 At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 80 2e-15 At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 79 4e-15 At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 77 1e-14 At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 76 2e-14 At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 75 4e-14 At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 73 2e-13 At4g02680.1 68417.m00363 tetratricopeptide repeat (TPR)-containi... 30 1.6 At4g14980.1 68417.m02302 DC1 domain-containing protein contains ... 27 8.6 At4g14510.1 68417.m02236 expressed protein contains Pfam domain,... 27 8.6 At1g01650.1 68414.m00083 protease-associated (PA) domain-contain... 27 8.6 >At1g06360.1 68414.m00672 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 299 Score = 86.2 bits (204), Expect = 2e-17 Identities = 35/92 (38%), Positives = 54/92 (58%) Frame = +1 Query: 394 FHAKWQTDLFAYILYVMSGLGITAGAHRLWAHKSYKAKCLYVLSLSSSIPWAFQDSALDW 573 F+ KW+ F ++LY ++ L IT HR AH+S+K L+ +A Q LDW Sbjct: 51 FNYKWEALRFGFVLYALTSLSITFSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDW 110 Query: 574 ARDHRMHHKYSETDADPHNATRGFFFSHIGWL 669 HR HH+++++D DPH+ GF+FSH+ W+ Sbjct: 111 VSIHRFHHQFTDSDRDPHSPIEGFWFSHVWWI 142 >At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from [Arabidopsis thaliana] Length = 307 Score = 83.0 bits (196), Expect = 2e-16 Identities = 39/105 (37%), Positives = 57/105 (54%) Frame = +1 Query: 355 LHIAALYGGYLFLFHAKWQTDLFAYILYVMSGLGITAGAHRLWAHKSYKAKCLYVLSLSS 534 +H AL + F + A W T LF Y + GLGIT HR AH+S+K L+ Sbjct: 50 VHSLALLAPFYFTWSALWVTFLF----YTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAY 105 Query: 535 SIPWAFQDSALDWARDHRMHHKYSETDADPHNATRGFFFSHIGWL 669 A Q +DW HR HH++++++ DPH+ GF+FSH+ W+ Sbjct: 106 CALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWI 150 >At1g06090.1 68414.m00638 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase GB:BAA25180 GI:2970034 (ADS1) from [Arabidopsis thaliana] Length = 299 Score = 81.4 bits (192), Expect = 5e-16 Identities = 34/92 (36%), Positives = 52/92 (56%) Frame = +1 Query: 394 FHAKWQTDLFAYILYVMSGLGITAGAHRLWAHKSYKAKCLYVLSLSSSIPWAFQDSALDW 573 F+ KW+ F IL +++ L IT HR HKS+K + S +A Q +DW Sbjct: 51 FNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDW 110 Query: 574 ARDHRMHHKYSETDADPHNATRGFFFSHIGWL 669 HR HH+++++D DPH+ GF+FSH+ W+ Sbjct: 111 VSTHRFHHQFTDSDRDPHSPIEGFWFSHVFWI 142 >At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180 GI:2970034 from [Arabidopsis thaliana] Length = 305 Score = 79.8 bits (188), Expect = 2e-15 Identities = 39/105 (37%), Positives = 57/105 (54%), Gaps = 3/105 (2%) Frame = +1 Query: 364 AALYGGYLFL---FHAKWQTDLFAYILYVMSGLGITAGAHRLWAHKSYKAKCLYVLSLSS 534 A+L+ +L L F+ W A I+Y + GLGIT HR AH+S+K + Sbjct: 44 ASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAY 103 Query: 535 SIPWAFQDSALDWARDHRMHHKYSETDADPHNATRGFFFSHIGWL 669 A Q +DW HR HH+++++D DPH+ GF+FSH+ WL Sbjct: 104 CGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWL 148 >At1g06100.1 68414.m00639 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 299 Score = 78.6 bits (185), Expect = 4e-15 Identities = 34/92 (36%), Positives = 51/92 (55%) Frame = +1 Query: 394 FHAKWQTDLFAYILYVMSGLGITAGAHRLWAHKSYKAKCLYVLSLSSSIPWAFQDSALDW 573 F+ KW+ F IL +++ L IT HR H+S+K + S A Q LDW Sbjct: 51 FNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDW 110 Query: 574 ARDHRMHHKYSETDADPHNATRGFFFSHIGWL 669 HR HH+++++D DPH+ GF+FSH+ W+ Sbjct: 111 VSIHRFHHQFTDSDRDPHSPIEGFWFSHVLWI 142 >At1g06350.1 68414.m00671 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 300 Score = 77.0 bits (181), Expect = 1e-14 Identities = 32/92 (34%), Positives = 51/92 (55%) Frame = +1 Query: 394 FHAKWQTDLFAYILYVMSGLGITAGAHRLWAHKSYKAKCLYVLSLSSSIPWAFQDSALDW 573 F+ KW+ F +L+ ++ L IT HR +H+S+K + S +A Q +DW Sbjct: 52 FNFKWEALRFGLVLFALTTLSITFSFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDW 111 Query: 574 ARDHRMHHKYSETDADPHNATRGFFFSHIGWL 669 HR HH+++++D DPH+ G FSHI W+ Sbjct: 112 VSIHRFHHQFTDSDRDPHSPKEGLLFSHILWI 143 >At1g06120.1 68414.m00641 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase GB:BAA25180 GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported by cDNA:gi_12083275_gb_AF332434.1_AF332434 Length = 299 Score = 75.8 bits (178), Expect = 2e-14 Identities = 31/92 (33%), Positives = 51/92 (55%) Frame = +1 Query: 394 FHAKWQTDLFAYILYVMSGLGITAGAHRLWAHKSYKAKCLYVLSLSSSIPWAFQDSALDW 573 F+ W+ FA ++ + + L IT HR H+S+K + S +A Q +DW Sbjct: 51 FNYTWEAFRFAAMVGISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDW 110 Query: 574 ARDHRMHHKYSETDADPHNATRGFFFSHIGWL 669 HR HH+++++D DPH+ GF+FSH+ W+ Sbjct: 111 VSTHRFHHQFTDSDRDPHSPIEGFWFSHVFWI 142 >At3g15850.1 68416.m02005 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 371 Score = 74.9 bits (176), Expect = 4e-14 Identities = 33/93 (35%), Positives = 52/93 (55%), Gaps = 1/93 (1%) Frame = +1 Query: 394 FHAKWQTDLFAYILYVMSGL-GITAGAHRLWAHKSYKAKCLYVLSLSSSIPWAFQDSALD 570 F W+ A+ LY+++GL GIT HR +HK++K + A Q + +D Sbjct: 121 FQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPID 180 Query: 571 WARDHRMHHKYSETDADPHNATRGFFFSHIGWL 669 W HR HH++ ++D DPH+ GF+FSH+ W+ Sbjct: 181 WVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWM 213 >At3g15870.1 68416.m02007 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 292 Score = 72.9 bits (171), Expect = 2e-13 Identities = 36/99 (36%), Positives = 57/99 (57%), Gaps = 1/99 (1%) Frame = +1 Query: 376 GGYLFLFHAKWQTDLFAYILYVMSGLGITAGAHRLWAHKSYKA-KCLYVLSLSSSIPWAF 552 G L A W +F +++++ +G+ IT HR +H+S+ K L L + AF Sbjct: 43 GSILLQLEAFW---VFPWLVFI-NGICITLSYHRNLSHRSFDLPKWLEYLFAYGGV-LAF 97 Query: 553 QDSALDWARDHRMHHKYSETDADPHNATRGFFFSHIGWL 669 Q ++W +HR HHK+ ET DPH+ T+GF+FSH+ W+ Sbjct: 98 QGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMAWI 136 >At4g02680.1 68417.m00363 tetratricopeptide repeat (TPR)-containing protein contains Pfam profile PF00515 TPR Domain Length = 888 Score = 29.9 bits (64), Expect = 1.6 Identities = 16/61 (26%), Positives = 30/61 (49%) Frame = +1 Query: 442 MSGLGITAGAHRLWAHKSYKAKCLYVLSLSSSIPWAFQDSALDWARDHRMHHKYSETDAD 621 ++ LG G HRLWA++ + + S+S + W +Q+ + D ++ T+ D Sbjct: 419 LARLGYIQG-HRLWAYEKLSS---VISSVSPPLGWMYQERSFYCEGDKKLEDLEKATELD 474 Query: 622 P 624 P Sbjct: 475 P 475 >At4g14980.1 68417.m02302 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 470 Score = 27.5 bits (58), Expect = 8.6 Identities = 12/24 (50%), Positives = 14/24 (58%) Frame = -1 Query: 287 VLDSPKSGVSAHFQREHHLHPLHW 216 V D S VS FQ + H HPL+W Sbjct: 291 VYDVRCSSVSEPFQHDLHPHPLYW 314 >At4g14510.1 68417.m02236 expressed protein contains Pfam domain, PF04581: Protein of unknown function (DUF578) Length = 932 Score = 27.5 bits (58), Expect = 8.6 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = -2 Query: 586 DDHVPNLKQNPEKPKELKMIRITRR 512 DDHV +KQ EK + K++R R Sbjct: 631 DDHVEEMKQEVEKVRSAKLVRKLER 655 >At1g01650.1 68414.m00083 protease-associated (PA) domain-containing protein contains protease associated (PA) domain, Pfam:PF02225 Length = 491 Score = 27.5 bits (58), Expect = 8.6 Identities = 15/34 (44%), Positives = 22/34 (64%) Frame = +1 Query: 361 IAALYGGYLFLFHAKWQTDLFAYILYVMSGLGIT 462 I+A++GG+LF HA L AY++ V G+G T Sbjct: 260 ISAIFGGFLFPDHA-----LQAYVILVYRGVGGT 288 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,938,365 Number of Sequences: 28952 Number of extensions: 323921 Number of successful extensions: 815 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 795 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 812 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1422784080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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