Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060852.seq
         (684 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d...   125   5e-30
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase...   123   2e-29
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo...    54   1e-08
SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe...    38   0.001
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ...    35   0.013
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p...    34   0.017
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe...    32   0.089
SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr...    27   2.5  
SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein Utp5|Schiz...    25   7.7  

>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
           dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 380

 Score =  125 bits (302), Expect = 5e-30
 Identities = 52/83 (62%), Positives = 66/83 (79%)
 Frame = +1

Query: 265 VTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQELYH 444
           VT+V+ GD V+ LY P+C TCKFC + KTNLC ++R+TQG+G+MPDGT RF C G  L H
Sbjct: 85  VTTVQVGDPVIALYTPECKTCKFCKSGKTNLCGRIRTTQGKGLMPDGTSRFSCNGNTLLH 144

Query: 445 FMGCSTFSQYTVVLEISLCKVER 513
           FMGCSTFS+YTVV +IS+  +ER
Sbjct: 145 FMGCSTFSEYTVVADISVVAIER 167



 Score =  123 bits (296), Expect = 3e-29
 Identities = 53/75 (70%), Positives = 62/75 (82%)
 Frame = +2

Query: 29  TVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGV 208
           T GK+I C AAVAW+   PLSIE ++V PP+  EVR+KI  +GVCHTDAYTLSGKDPEG+
Sbjct: 6   TAGKIINCKAAVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSGVCHTDAYTLSGKDPEGL 65

Query: 209 FPVVLGHEGGGIVES 253
           FPV+LGHEG GIVES
Sbjct: 66  FPVILGHEGAGIVES 80



 Score = 53.6 bits (123), Expect = 3e-08
 Identities = 22/35 (62%), Positives = 27/35 (77%)
 Frame = +3

Query: 516 APLDKVCLLGCGVPTGYGAALNTAKVEPGSNCAIF 620
           APLD VCLLGCG+ TGYGAA  TA ++ G + A+F
Sbjct: 169 APLDSVCLLGCGITTGYGAATITADIKEGDSVAVF 203


>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score =  123 bits (297), Expect = 2e-29
 Identities = 50/81 (61%), Positives = 64/81 (79%)
 Frame = +1

Query: 265 VTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQELYH 444
           V +V+PGDHV+ LY P+C  CKFC + KTNLC K+R TQG+G+MPDGT RF C+ + L H
Sbjct: 82  VINVRPGDHVILLYTPECKECKFCRSGKTNLCSKIRETQGRGLMPDGTSRFSCRDKTLLH 141

Query: 445 FMGCSTFSQYTVVLEISLCKV 507
           +MGCS+FSQYTVV +ISL  +
Sbjct: 142 YMGCSSFSQYTVVADISLVAI 162



 Score =  121 bits (292), Expect = 8e-29
 Identities = 58/88 (65%), Positives = 65/88 (73%)
 Frame = +2

Query: 23  MSTVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPE 202
           MS  GK I C AAVAW A +PLSIE+I+V PPKA EVRVK+  + VCHTDAYTLSG DPE
Sbjct: 1   MSFEGKTITCKAAVAWGAKEPLSIEDIQVAPPKAHEVRVKVDWSAVCHTDAYTLSGVDPE 60

Query: 203 GVFPVVLGHEGGGIVESAVRESLQSSPG 286
           G FP+VLGHEG GIVES     +   PG
Sbjct: 61  GAFPIVLGHEGAGIVESIGEGVINVRPG 88



 Score = 52.0 bits (119), Expect = 8e-08
 Identities = 25/57 (43%), Positives = 32/57 (56%)
 Frame = +3

Query: 513 AAPLDKVCLLGCGVPTGYGAALNTAKVEPGSNCAIFXXXXXXXXXXXXCKAGSANRI 683
           +APL  +CLLGCGV TG+GA  ++AKVE GS  A+               A  A+RI
Sbjct: 165 SAPLRSICLLGCGVTTGFGAVTHSAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRI 221


>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
           Adh1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 350

 Score = 54.4 bits (125), Expect = 1e-08
 Identities = 32/70 (45%), Positives = 39/70 (55%), Gaps = 3/70 (4%)
 Frame = +2

Query: 47  KCLAAVAWEAGKPLSI--EEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDP-EGVFPV 217
           K LAAV    G P ++  EE+ V  P   EV V I  TGVCHTD + L G  P     P+
Sbjct: 6   KQLAAVFHTHGGPENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQGDWPLPAKMPL 65

Query: 218 VLGHEGGGIV 247
           + GHEG G+V
Sbjct: 66  IGGHEGAGVV 75


>SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 325

 Score = 37.9 bits (84), Expect = 0.001
 Identities = 18/56 (32%), Positives = 30/56 (53%)
 Frame = +2

Query: 86  LSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGVFPVVLGHEGGGIVES 253
           + I+ + +  PK GE+ VKI A  +  +D    +G  P  V+P ++G +  G V S
Sbjct: 23  IEIQSVPIPQPKNGELLVKIEAAAINPSDLMNATGGFPYTVYPRIVGRDYAGTVIS 78


>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 423

 Score = 34.7 bits (76), Expect = 0.013
 Identities = 24/69 (34%), Positives = 36/69 (52%), Gaps = 4/69 (5%)
 Frame = +2

Query: 53  LAAVAWEAGKPLSIEEIEVDPPKAG---EVRVKITATGVCH-TDAYTLSGKDPEGVFPVV 220
           + A  W+   PL+++  EV  P      +V VK TA  +C  +D++  SG+ P      +
Sbjct: 37  MKACVWDG--PLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPGIEKGAI 94

Query: 221 LGHEGGGIV 247
           LGHE  GIV
Sbjct: 95  LGHESCGIV 103


>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 360

 Score = 34.3 bits (75), Expect = 0.017
 Identities = 19/56 (33%), Positives = 29/56 (51%), Gaps = 3/56 (5%)
 Frame = +2

Query: 128 EVRVKITATGVCHTDA-YTLSGKDPEGVF--PVVLGHEGGGIVESAVRESLQSSPG 286
           +V+V I ATG+C +D  Y   G   + +   P++LGHE  G+V    +      PG
Sbjct: 31  QVKVAIKATGICGSDVHYWKEGGIGDFILKKPMILGHESAGVVVEVGKGVSSLKPG 86



 Score = 29.9 bits (64), Expect = 0.36
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = +1

Query: 265 VTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLC 360
           V+S+KPGD V       C  C +C + + NLC
Sbjct: 80  VSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLC 111


>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 346

 Score = 31.9 bits (69), Expect = 0.089
 Identities = 24/58 (41%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
 Frame = +2

Query: 86  LSIEEIEVDPPKA--GEVRVKITATGVCHTDAYTLSGKDPEGV-FPVVLGHEGGGIVE 250
           L  EE EV P K   GEV VK+ A  + + D     G  P  +  PVV G +G GI+E
Sbjct: 17  LKPEEYEV-PQKLNPGEVLVKLKAASLNYRDLIITKGLYPLPLQLPVVPGSDGAGIIE 73


>SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1250

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
 Frame = +1

Query: 283  GDHVVPLY-VPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQEL 438
            G HVV L  V   N C   +  KT    KV   +   + P G+R++ C G  L
Sbjct: 1036 GKHVVRLLQVRDANGCAASIT-KTQPAAKVSVVEMASLAPLGSRQYYCVGDRL 1087


>SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein
           Utp5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 666

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 15/38 (39%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
 Frame = +2

Query: 47  KCLAAVAWEAGKPL-SIEEIEVDPPKAGEVRVKITATG 157
           K ++ +AWE  KPL + E+I  D   +GE+ V  T +G
Sbjct: 63  KVISCIAWEQ-KPLYASEQITTDISGSGEILVLGTNSG 99


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,750,438
Number of Sequences: 5004
Number of extensions: 56405
Number of successful extensions: 165
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)