Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060850.seq
(683 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P14868 Cluster: Aspartyl-tRNA synthetase, cytoplasmic; ... 55 1e-06
UniRef50_O74407 Cluster: Aspartate-tRNA ligase; n=1; Schizosacch... 50 5e-05
UniRef50_UPI00015A65C6 Cluster: Aspartyl-tRNA synthetase, cytopl... 43 0.008
UniRef50_Q8BJY7 Cluster: 13 days embryo forelimb cDNA, RIKEN ful... 42 0.019
UniRef50_Q4Q7R2 Cluster: Aspartyl-tRNA synthetase, putative; n=7... 38 0.17
UniRef50_A0CH59 Cluster: Chromosome undetermined scaffold_18, wh... 38 0.17
UniRef50_Q559M8 Cluster: Aspartyl-tRNA synthetase; n=3; Eukaryot... 36 0.70
UniRef50_A3FPN1 Cluster: Aspartate--tRNA ligase; n=3; Cryptospor... 34 3.7
UniRef50_O77320 Cluster: Putative uncharacterized protein MAL3P3... 33 8.6
>UniRef50_P14868 Cluster: Aspartyl-tRNA synthetase, cytoplasmic;
n=35; Eukaryota|Rep: Aspartyl-tRNA synthetase,
cytoplasmic - Homo sapiens (Human)
Length = 501
Score = 55.2 bits (127), Expect = 1e-06
Identities = 36/91 (39%), Positives = 47/91 (51%)
Frame = +2
Query: 323 MESEPDFSEGLYGXMKMIXSSXXHRDRVYTXVENLSIKMDGQTVWVRARLQTSRANRQAV 502
M++ D+++ YG MI S DRV V +L+I+ + VWVRAR+ TSRA +
Sbjct: 18 MDAAEDYAKERYGISSMIQSQEKP-DRVLVRVRDLTIQKADEVVWVRARVHTSRAKGKQC 76
Query: 503 XCVLRQTSSTVQAAG*R*RASGKXSKQXVKF 595
VLRQ VQA SKQ VKF
Sbjct: 77 FLVLRQQQFNVQALV---AVGDHASKQMVKF 104
>UniRef50_O74407 Cluster: Aspartate-tRNA ligase; n=1;
Schizosaccharomyces pombe|Rep: Aspartate-tRNA ligase -
Schizosaccharomyces pombe (Fission yeast)
Length = 580
Score = 50.0 bits (114), Expect = 5e-05
Identities = 35/92 (38%), Positives = 50/92 (54%), Gaps = 1/92 (1%)
Frame = +2
Query: 329 SEPDFSEGLYGXMKMIXSSXXHRDRVYTXVENLSIKMDGQTVWVRARLQTSR-ANRQAVX 505
+E D+S G YG + + S+ R YT + ++S K DGQTV +RAR+ TSR +
Sbjct: 67 NEIDYSAGKYGDLPLNRSTA-RPGRTYTQISDISAKNDGQTVLLRARVYTSRLQGNKMCF 125
Query: 506 CVLRQTSSTVQAAG*R*RASGKXSKQXVKFTG 601
LRQ T+QA + + SKQ VK+ G
Sbjct: 126 FSLRQKYDTIQALAVVNKDT--ISKQMVKWCG 155
>UniRef50_UPI00015A65C6 Cluster: Aspartyl-tRNA synthetase,
cytoplasmic (EC 6.1.1.12) (Aspartate--tRNA ligase)
(AspRS) (Cell proliferation-inducing gene 40 protein).;
n=2; Danio rerio|Rep: Aspartyl-tRNA synthetase,
cytoplasmic (EC 6.1.1.12) (Aspartate--tRNA ligase)
(AspRS) (Cell proliferation-inducing gene 40 protein). -
Danio rerio
Length = 536
Score = 42.7 bits (96), Expect = 0.008
Identities = 21/52 (40%), Positives = 31/52 (59%)
Frame = +2
Query: 332 EPDFSEGLYGXMKMIXSSXXHRDRVYTXVENLSIKMDGQTVWVRARLQTSRA 487
E DF++ YG M+ S DR V++L+++ Q +WVRAR+ TSRA
Sbjct: 51 EDDFAKDRYGICPMVQSQQK-LDRALVRVQDLTLEKAEQQIWVRARIHTSRA 101
>UniRef50_Q8BJY7 Cluster: 13 days embryo forelimb cDNA, RIKEN
full-length enriched library, clone:5930400O16
product:SIMILAR TO ASPARTYL-TRNA SYNTHETASE homolog;
n=5; Mammalia|Rep: 13 days embryo forelimb cDNA, RIKEN
full-length enriched library, clone:5930400O16
product:SIMILAR TO ASPARTYL-TRNA SYNTHETASE homolog -
Mus musculus (Mouse)
Length = 87
Score = 41.5 bits (93), Expect = 0.019
Identities = 21/55 (38%), Positives = 33/55 (60%)
Frame = +2
Query: 323 MESEPDFSEGLYGXMKMIXSSXXHRDRVYTXVENLSIKMDGQTVWVRARLQTSRA 487
+++ D+++ YG MI S DRV V++L+++ VWVRAR+ TSRA
Sbjct: 18 VDAAEDYAKERYGISSMIQSQEKP-DRVLVRVKDLTVQKADDVVWVRARVHTSRA 71
>UniRef50_Q4Q7R2 Cluster: Aspartyl-tRNA synthetase, putative; n=7;
Trypanosomatidae|Rep: Aspartyl-tRNA synthetase, putative
- Leishmania major
Length = 550
Score = 38.3 bits (85), Expect = 0.17
Identities = 26/87 (29%), Positives = 44/87 (50%), Gaps = 1/87 (1%)
Frame = +2
Query: 347 EGLYGXMKMIXSSXXHRDRVYTXVENLSI-KMDGQTVWVRARLQTSRANRQAVXCVLRQT 523
+ ++G M+ S+ ++ R + V LS ++ +TV +RAR+ T+R + VLR
Sbjct: 46 KAVFGAAPMVQSTT-YKSRTHIPVSELSRPELVDKTVLIRARVSTTRKKGKMAFMVLRDG 104
Query: 524 SSTVQAAG*R*RASGKXSKQXVKFTGQ 604
S +VQA G K+ + F GQ
Sbjct: 105 SDSVQAMA---AVEGDVPKEMIDFMGQ 128
>UniRef50_A0CH59 Cluster: Chromosome undetermined scaffold_18, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_18,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 506
Score = 38.3 bits (85), Expect = 0.17
Identities = 27/93 (29%), Positives = 46/93 (49%), Gaps = 1/93 (1%)
Frame = +2
Query: 326 ESEPDFSEG-LYGXMKMIXSSXXHRDRVYTXVENLSIKMDGQTVWVRARLQTSRANRQAV 502
E + D ++G YG + +I S +R + V ++ G+T+ +RAR+ SR +
Sbjct: 8 EKQQDINDGDRYGDVSLIQQSQK-TNRQWMKVSQINESTIGKTILLRARIHNSRMKGKIA 66
Query: 503 XCVLRQTSSTVQAAG*R*RASGKXSKQXVKFTG 601
VLR+ T+QA + G Q +K+TG
Sbjct: 67 FLVLRENWYTIQAVLTKGEQIG---SQMIKYTG 96
>UniRef50_Q559M8 Cluster: Aspartyl-tRNA synthetase; n=3;
Eukaryota|Rep: Aspartyl-tRNA synthetase - Dictyostelium
discoideum AX4
Length = 576
Score = 36.3 bits (80), Expect = 0.70
Identities = 27/69 (39%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Frame = +2
Query: 401 RVYTXVENLSIKMDGQTVWVRARLQTSRANRQAVXCV-LRQTSSTVQAAG*R*RASGKXS 577
R +T V L+ + G++V +RARL TSR + V LR TVQA G S
Sbjct: 84 REWTDVSQLTESLVGKSVLIRARLSTSRLQGANLCFVQLRDGLYTVQAVV---AKGGSNS 140
Query: 578 KQXVKFTGQ 604
K V+F GQ
Sbjct: 141 KSMVQFVGQ 149
>UniRef50_A3FPN1 Cluster: Aspartate--tRNA ligase; n=3;
Cryptosporidium|Rep: Aspartate--tRNA ligase -
Cryptosporidium parvum Iowa II
Length = 532
Score = 33.9 bits (74), Expect = 3.7
Identities = 18/68 (26%), Positives = 34/68 (50%)
Frame = +2
Query: 398 DRVYTXVENLSIKMDGQTVWVRARLQTSRANRQAVXCVLRQTSSTVQAAG*R*RASGKXS 577
+R ++ ++ L+ G+ +W+R R+ SR +LRQT +Q A+ S
Sbjct: 70 NRTFSKIKELNSNQKGEKIWLRGRITESRCKGSLGFVLLRQTFYRLQLVV---DANNGSS 126
Query: 578 KQXVKFTG 601
K+ +K+ G
Sbjct: 127 KEMIKWLG 134
>UniRef50_O77320 Cluster: Putative uncharacterized protein MAL3P3.3;
n=3; Plasmodium|Rep: Putative uncharacterized protein
MAL3P3.3 - Plasmodium falciparum (isolate 3D7)
Length = 3724
Score = 32.7 bits (71), Expect = 8.6
Identities = 15/35 (42%), Positives = 23/35 (65%)
Frame = +1
Query: 34 N*KRYIDNKDISTYNNQILLDRHVHKK*NLNFLVN 138
N KR +NK +S YN+ ++++HV K+ N N L N
Sbjct: 169 NFKRTAENKKLSLYNDTNIIEKHVIKEKNKNTLKN 203
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 507,173,948
Number of Sequences: 1657284
Number of extensions: 8106074
Number of successful extensions: 16322
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 15943
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16316
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 53305790091
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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