Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060848.seq
         (685 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   135   5e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    88   1e-18
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    87   2e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    68   1e-12
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe...    28   1.4  
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   2.5  
SPCC162.10 |ppk33||serine/threonine protein kinase Ppk33 |Schizo...    27   2.5  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   3.3  
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        26   4.4  
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces...    26   4.4  
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c...    26   4.4  
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos...    26   5.8  
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    25   7.7  
SPBC1A4.07c |||U3 snoRNP-associated protein Sof1|Schizosaccharom...    25   7.7  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  135 bits (327), Expect = 5e-33
 Identities = 58/72 (80%), Positives = 66/72 (91%)
 Frame = +1

Query: 256 VDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAE 435
           VDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+EAE
Sbjct: 66  VDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAE 125

Query: 436 SCDCLQGFQLTH 471
           +CD LQGFQLTH
Sbjct: 126 ACDALQGFQLTH 137



 Score =  113 bits (273), Expect = 2e-26
 Identities = 47/64 (73%), Positives = 55/64 (85%)
 Frame = +2

Query: 62  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 241
           MREIVHIQAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 242 PRAI 253
           PRA+
Sbjct: 61  PRAV 64



 Score = 91.5 bits (217), Expect = 1e-19
 Identities = 42/61 (68%), Positives = 48/61 (78%)
 Frame = +3

Query: 501 GHPLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVEKHRRNYCIDKRGL 680
           G  L+SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVE     +CID   L
Sbjct: 148 GTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEAL 207

Query: 681 S 683
           S
Sbjct: 208 S 208


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 88.2 bits (209), Expect = 1e-18
 Identities = 36/72 (50%), Positives = 52/72 (72%)
 Frame = +1

Query: 256 VDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAE 435
           VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++DSVL+ +R+ A+
Sbjct: 72  VDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMAD 131

Query: 436 SCDCLQGFQLTH 471
           +C  LQGF + H
Sbjct: 132 NCSGLQGFLVFH 143



 Score = 51.2 bits (117), Expect = 1e-07
 Identities = 25/70 (35%), Positives = 37/70 (52%), Gaps = 6/70 (8%)
 Frame = +2

Query: 62  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 223
           MRE++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 224 SGGKYVPRAI 253
             GK+VPR+I
Sbjct: 61  GQGKFVPRSI 70



 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 19/56 (33%), Positives = 33/56 (58%)
 Frame = +3

Query: 501 GHPLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVEKHRRNYCID 668
           G  L+ ++  EY  +    +SV P+P+VS +VVEPYN+ L+ H  ++     + +D
Sbjct: 154 GALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVD 209


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 87.0 bits (206), Expect = 2e-18
 Identities = 36/72 (50%), Positives = 50/72 (69%)
 Frame = +1

Query: 256 VDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAE 435
           VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V D +R+ A+
Sbjct: 68  VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127

Query: 436 SCDCLQGFQLTH 471
           +C  LQGF + H
Sbjct: 128 NCSGLQGFLVFH 139



 Score = 57.2 bits (132), Expect = 2e-09
 Identities = 28/66 (42%), Positives = 38/66 (57%), Gaps = 2/66 (3%)
 Frame = +2

Query: 62  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 235
           MREI+ I  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 236 YVPRAI 253
           YVPR+I
Sbjct: 61  YVPRSI 66



 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 19/57 (33%), Positives = 34/57 (59%)
 Frame = +3

Query: 498 YGHPLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVEKHRRNYCID 668
           +G  L+ ++  EY  +    +SV P+P+VS +VVEPYN+ L+ H  ++     + +D
Sbjct: 149 FGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVD 205


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 68.1 bits (159), Expect = 1e-12
 Identities = 28/63 (44%), Positives = 44/63 (69%)
 Frame = +2

Query: 65  REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 244
           REI+ +QAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 245 RAI 253
           RAI
Sbjct: 63  RAI 65



 Score = 65.7 bits (153), Expect = 6e-12
 Identities = 26/74 (35%), Positives = 48/74 (64%), Gaps = 2/74 (2%)
 Frame = +1

Query: 256 VDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKE 429
           +DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++D++ +E
Sbjct: 67  IDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDRE 125

Query: 430 AESCDCLQGFQLTH 471
           A+  D L+GF L H
Sbjct: 126 ADGSDSLEGFSLLH 139



 Score = 46.4 bits (105), Expect = 4e-06
 Identities = 21/46 (45%), Positives = 32/46 (69%), Gaps = 1/46 (2%)
 Frame = +3

Query: 501 GHPLISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQL 635
           G  L+ ++ + YP +I+ TYSV P S  VSD VV+PYN+ L++ +L
Sbjct: 150 GSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRL 195


>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 969

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 20/56 (35%), Positives = 31/56 (55%), Gaps = 8/56 (14%)
 Frame = +3

Query: 492 FRYGHPLISKIREEYP-DRIMNTYSVVP--SPKVSDTVVE---PYN--ATLSVHQL 635
           ++Y H + SK++ EYP D+++   SV+    PK    VVE   P N  A L+ H +
Sbjct: 391 YQYAHVVASKLQREYPRDKVLYYSSVLTEFDPKGIQEVVESLRPNNFFAILAAHSI 446


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +2

Query: 101 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 202
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPCC162.10 |ppk33||serine/threonine protein kinase Ppk33
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 338

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 12/24 (50%), Positives = 17/24 (70%)
 Frame = +2

Query: 584 VGHSSRTVQRHTFSSSIS*KTQTK 655
           +GHSS+ +Q H F  SIS +T +K
Sbjct: 289 LGHSSKKLQTHPFFCSISLETVSK 312


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +1

Query: 295 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 396
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +1

Query: 364 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 453
           A+GH   G ELV +  D +RK++E+   L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212


>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 967

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 16/43 (37%), Positives = 21/43 (48%)
 Frame = +1

Query: 229 RQVRAPRHPVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGN 357
           R+V AP+H      GT    RS     +FR ++  FGQ   GN
Sbjct: 486 REVFAPKH-ARRRLGTRFHSRSSHRPSVFRDNSVAFGQLDNGN 527


>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 807

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = -3

Query: 437 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 300
           ++A+ RTTS T+    +PS       L P P    +  S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432


>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1063

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 12/44 (27%), Positives = 20/44 (45%)
 Frame = +1

Query: 316  RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 447
            RP   +F     G++   G   +  E  D ++ +  +  ESCDC
Sbjct: 955  RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = -3

Query: 353 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 258
           P+   PK  L  R I P GPE  +  + GS S
Sbjct: 20  PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51


>SPBC1A4.07c |||U3 snoRNP-associated protein
           Sof1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 436

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 14/47 (29%), Positives = 22/47 (46%)
 Frame = +3

Query: 534 YPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVEKHRRNYCIDKR 674
           Y D +    SV  SP   + V   Y+ T+ ++ + E H R+    KR
Sbjct: 269 YKDHVSAVMSVDFSPTGQEFVSGSYDKTIRIYNVREGHSRDVYHTKR 315


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,663,208
Number of Sequences: 5004
Number of extensions: 51930
Number of successful extensions: 157
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)