Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060847.seq
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 31 0.94
At5g23980.1 68418.m02818 ferric-chelate reductase, putative simi... 28 5.0
At5g04500.1 68418.m00449 glycosyltransferase family protein 47 l... 28 5.0
At4g31210.1 68417.m04432 DNA topoisomerase family protein simila... 28 6.6
At3g13760.1 68416.m01736 DC1 domain-containing protein contains ... 28 6.6
At1g51450.1 68414.m05791 SPla/RYanodine receptor (SPRY) domain-c... 28 6.6
At2g46380.1 68415.m05773 hypothetical protein weak similarity to... 27 8.8
At1g55350.4 68414.m06326 calpain-type cysteine protease family i... 27 8.8
At1g55350.3 68414.m06325 calpain-type cysteine protease family i... 27 8.8
At1g55350.2 68414.m06324 calpain-type cysteine protease family i... 27 8.8
At1g55350.1 68414.m06323 calpain-type cysteine protease family i... 27 8.8
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 30.7 bits (66), Expect = 0.94
Identities = 17/37 (45%), Positives = 22/37 (59%)
Frame = -1
Query: 403 LVVEAAVHGRKLGVFEESTFS*APRSYVFEVLIRSRT 293
L+ EAA +G +L VF E+ PR FE+ I SRT
Sbjct: 60 LLSEAAENGSQLVVFPEAFIGGYPRGSTFELAIGSRT 96
>At5g23980.1 68418.m02818 ferric-chelate reductase, putative similar
to ferric-chelate reductase (FRO1) [Pisum sativum]
GI:15341529; contains Pfam profile PF01794: Ferric
reductase like transmembrane component
Length = 699
Score = 28.3 bits (60), Expect = 5.0
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -3
Query: 149 SLIKQLFSRALYKGAKIPTAILACHSKLAHFH 54
S+I++L S++ K K+P +L C K H+H
Sbjct: 429 SVIRELISQSQNKSTKLPDVLLVCSFK--HYH 458
>At5g04500.1 68418.m00449 glycosyltransferase family protein 47 low
similarity to Exostosin-like 2, Homo sapiens
[SP|Q9UBQ6], EXTL2, Mus musculus [GI:10443633]
Length = 765
Score = 28.3 bits (60), Expect = 5.0
Identities = 15/39 (38%), Positives = 20/39 (51%)
Frame = -1
Query: 574 LGRITCDEVSSWQRQPTANNRPLSLLDVEATVAFDEKLR 458
LG + C S+W A R +LL++E F EKLR
Sbjct: 415 LGVVNCIVPSTWCMNYYAGKRTDALLNLETAGLFSEKLR 453
>At4g31210.1 68417.m04432 DNA topoisomerase family protein similar
to DNA Topoisomerase I (SP:Q9X3X7) {Zymomonas mobilis}
Length = 1280
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = -1
Query: 685 GRRSSMTTFQPKRP*LPETEMEDAPHNLHRNSDHRVRLGR 566
G ++S T P P + T +DA + LH ++ H ++L +
Sbjct: 719 GTKTSTTRKNPPTPYITSTLQQDAANKLHFSTAHTMKLAQ 758
>At3g13760.1 68416.m01736 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 652
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/49 (28%), Positives = 25/49 (51%)
Frame = -1
Query: 676 SSMTTFQPKRP*LPETEMEDAPHNLHRNSDHRVRLGRITCDEVSSWQRQ 530
S+ + ++P + D+PHNL D ++ LG TC ++ +RQ
Sbjct: 30 STKEMYDAEKPRCLLSHPADSPHNLSVGCDPKLPLGCFTCGGKTTVRRQ 78
>At1g51450.1 68414.m05791 SPla/RYanodine receptor (SPRY)
domain-containing protein low similarity to DEAD box
protein DDX1 [Gallus gallus] GI:16323037, ryanodine
receptor [Caenorhabditis elegans] GI:1871447; contains
Pfam profile PF00622: SPRY domain
Length = 509
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/37 (37%), Positives = 23/37 (62%)
Frame = +2
Query: 566 SSKSYPMIRVSMKIMRGVFHFRFWQLRALGLERGHRR 676
SSK Y M+R + ++ G ++F ++ +LG E GH R
Sbjct: 295 SSKGYRMVRATRGVVEGAWYFEI-KVLSLG-ETGHTR 329
>At2g46380.1 68415.m05773 hypothetical protein weak similarity to
extra-large G-protein [Arabidopsis thaliana] GI:3201682
Length = 732
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 362 DPEFPAVDRSLYYKESLDRPITWLRPGEISDNPQL 466
+P F +RS + SL P T++ PG S PQL
Sbjct: 291 NPGFYPHERSFGFGTSLHSPRTFIPPGSQSPGPQL 325
>At1g55350.4 68414.m06326 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +1
Query: 508 VGDCWLLAAVANLTLHRKLFFQVVPDDQSFDEDYAGRLPFPFLAVTGAWV 657
+GDCW L+AVA LT ++ ++ + + + Y R + G WV
Sbjct: 1758 LGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVR-----FCIQGEWV 1802
>At1g55350.3 68414.m06325 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +1
Query: 508 VGDCWLLAAVANLTLHRKLFFQVVPDDQSFDEDYAGRLPFPFLAVTGAWV 657
+GDCW L+AVA LT ++ ++ + + + Y R + G WV
Sbjct: 1758 LGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVR-----FCIQGEWV 1802
>At1g55350.2 68414.m06324 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +1
Query: 508 VGDCWLLAAVANLTLHRKLFFQVVPDDQSFDEDYAGRLPFPFLAVTGAWV 657
+GDCW L+AVA LT ++ ++ + + + Y R + G WV
Sbjct: 1758 LGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVR-----FCIQGEWV 1802
>At1g55350.1 68414.m06323 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +1
Query: 508 VGDCWLLAAVANLTLHRKLFFQVVPDDQSFDEDYAGRLPFPFLAVTGAWV 657
+GDCW L+AVA LT ++ ++ + + + Y R + G WV
Sbjct: 1758 LGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVR-----FCIQGEWV 1802
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,389,952
Number of Sequences: 28952
Number of extensions: 329997
Number of successful extensions: 801
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 769
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 800
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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