BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060843.seq (662 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 2.4 SPBC32F12.01c ||SPBC685.10c|inositol phosphosphingolipid phospho... 26 4.2 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 4.2 SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 26 5.6 >SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 391 Score = 27.1 bits (57), Expect = 2.4 Identities = 14/36 (38%), Positives = 17/36 (47%) Frame = +2 Query: 98 HNGSPSGNRHQQFPSQASTLPISGKFQANPIAQSTR 205 +NG PSG R + ST IS F P A +R Sbjct: 10 NNGLPSGEREESETPGGSTSIISSSFLLQPRASDSR 45 >SPBC32F12.01c ||SPBC685.10c|inositol phosphosphingolipid phospholipase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 424 Score = 26.2 bits (55), Expect = 4.2 Identities = 10/32 (31%), Positives = 17/32 (53%) Frame = +2 Query: 407 DITRYPVSNPHPVETEVAFMHDALMFITPSQI 502 D P+S PH + T ++DA + + P Q+ Sbjct: 179 DFNIQPLSVPHEIITSYGLVNDAWLSVYPDQV 210 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 26.2 bits (55), Expect = 4.2 Identities = 27/98 (27%), Positives = 42/98 (42%), Gaps = 1/98 (1%) Frame = +2 Query: 140 SQASTLPISGKFQANPIAQSTRHRSHLLWHFTSSSCRS*AIETHLLFEHWNHLARVPSTV 319 + +STL S + P A S+ H S L H +SSS +S + F H + +R S Sbjct: 378 TSSSTLTSSSSSSSRP-ASSSSHSSSLSSHKSSSSSKSSSAPVSSAFYHNSTSSRSSSHS 436 Query: 320 MYMKPEKFQKLQQLNPNFASLI-NFRHTPKDITRYPVS 430 L + +SL+ + HT + T Y V+ Sbjct: 437 SSHSLSSLSSKPILTASSSSLLTSSSHTYERSTVYVVT 474 >SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 321 Score = 25.8 bits (54), Expect = 5.6 Identities = 9/28 (32%), Positives = 19/28 (67%) Frame = +3 Query: 141 HKLQLYPYQVNSKLIPLLNQLGIGVTSY 224 H+++L+P+ ++ + +LGI VT+Y Sbjct: 188 HQMELHPFLPQTEFVEKHKKLGIHVTAY 215 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,846,634 Number of Sequences: 5004 Number of extensions: 63719 Number of successful extensions: 149 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 144 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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