BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060834.seq (686 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g44800.1 68415.m05575 oxidoreductase, 2OG-Fe(II) oxygenase fa... 31 0.95 At3g15050.1 68416.m01904 calmodulin-binding family protein simil... 30 1.3 At1g19030.1 68414.m02369 hypothetical protein 29 2.2 At1g24450.1 68414.m03080 ribonuclease III family protein contain... 27 8.8 >At2g44800.1 68415.m05575 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavonol synthase SP|Q96330 {Arabidopsis thaliana}, SP|Q07512 {Petunia hybrida}; contains Pfam profile PF03171: oxidoreductase, 2OG-Fe(II) oxygenase family Length = 357 Score = 30.7 bits (66), Expect = 0.95 Identities = 17/64 (26%), Positives = 30/64 (46%), Gaps = 2/64 (3%) Frame = +2 Query: 197 PRLPRRKIRNNEYSNAFGNSMGDIGLTTKVLDRLIIHQDPLRTSCLDQDQDQC--LGLYP 370 P++P R + A G+S+G T V+D ++HQ LR+ + + C G + Sbjct: 25 PQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGFFQ 84 Query: 371 IKTH 382 + H Sbjct: 85 VINH 88 >At3g15050.1 68416.m01904 calmodulin-binding family protein similar to SF16 protein [Helianthus annuus] GI:560150; contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 259 Score = 30.3 bits (65), Expect = 1.3 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = -3 Query: 351 WSWSWSKHEVRRGSWWMIKRSRTLVVRPISPIEFP 247 W WSW + + W + R++ VV+PI P + P Sbjct: 199 WGWSWKERWIAARPWEI--RAQCYVVKPIKPSKKP 231 >At1g19030.1 68414.m02369 hypothetical protein Length = 398 Score = 29.5 bits (63), Expect = 2.2 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = -3 Query: 381 WVLIG*RPKHWSWSWSKHEVRRGSWWMIKRSRT 283 W ++ R W K+ +RRGS+W++K + T Sbjct: 121 WRILSARRSLWVELVKKYLIRRGSFWLVKENTT 153 >At1g24450.1 68414.m03080 ribonuclease III family protein contains similarity to Swiss-Prot:P51837 ribonuclease III (EC 3.1.26.3) (RNase III) [Coxiella burnetii] Length = 191 Score = 27.5 bits (58), Expect = 8.8 Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 2/54 (3%) Frame = +2 Query: 263 DIGLTTKVLDRLIIHQDPLRTSC-LDQDQDQCLGLYP-IKTHSEEDLVRMALLC 418 DI +++K L RLI + +SC LD D+ LGL I+ ++ D A+LC Sbjct: 102 DIDISSKALGRLISEVSNVESSCALDGDR---LGLGKIIRVSTKTDASNSAILC 152 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,768,250 Number of Sequences: 28952 Number of extensions: 305945 Number of successful extensions: 825 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 795 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 825 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1457719448 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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