BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060832.seq (688 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 58 2e-09 SPAC4H3.05 |srs2||ATP-dependent DNA helicase, UvrD subfamily|Sch... 30 0.36 SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 27 2.5 SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces... 26 4.4 SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 26 5.9 SPBC83.03c |tas3||RITS complex subunit 3 |Schizosaccharomyces po... 26 5.9 SPCC794.08 |||HEAT repeat protein, unknown biological role|Schiz... 25 7.8 SPAC9.05 |mfh1||ATP-dependent DNA helicase Mfh1 |Schizosaccharom... 25 7.8 >SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 499 Score = 57.6 bits (133), Expect = 2e-09 Identities = 26/53 (49%), Positives = 37/53 (69%) Frame = +3 Query: 525 LLQEVTIKSFLLSKGPQFAITIKKLETDLTSDMLLSGSWKTLEFKPYNFDALG 683 L++ I F L KGP F++ I+KL TDLT++M+ S SW++ +FK YNF A G Sbjct: 163 LVERNKIMYFSLRKGPNFSLQIEKLNTDLTAEMITSRSWESAKFKSYNFAAEG 215 Score = 51.2 bits (117), Expect = 1e-07 Identities = 40/112 (35%), Positives = 52/112 (46%), Gaps = 3/112 (2%) Frame = +1 Query: 250 CYNGSHEAVLYRSI--PDSGVPQSEAMKMVPN-AKVGFSKAMSSGWIYIDKSSGPPLVKR 420 C NGSHEA +Y I SG+ E K + N A +G +A GWI K G LVK Sbjct: 72 CANGSHEAKVYNEICASMSGLNIGELKKKLGNSAGIGQGRAFKLGWI---KKDGDKLVK- 127 Query: 421 KVDSITDIVQENLSEIRKGIDNLSENVRNAIKKGNFYKR*PLKASCCRRAPN 576 DSITD + LSEI++ +KK +R + R+ PN Sbjct: 128 NTDSITDETPKVLSEIKEHGTISDSKTLTDLKKRKLVERNKIMYFSLRKGPN 179 >SPAC4H3.05 |srs2||ATP-dependent DNA helicase, UvrD subfamily|Schizosaccharomyces pombe|chr 1|||Manual Length = 887 Score = 29.9 bits (64), Expect = 0.36 Identities = 23/74 (31%), Positives = 34/74 (45%) Frame = +1 Query: 298 SGVPQSEAMKMVPNAKVGFSKAMSSGWIYIDKSSGPPLVKRKVDSITDIVQENLSEIRKG 477 SG S A K + K G +SS W+ D++ ++KR +DS+ S IR G Sbjct: 89 SGTFHSIAYKYL--VKYGKHIGLSSNWLIADRNDTQAIMKRLLDSLKKAKNPIASGIR-G 145 Query: 478 IDNLSENVRNAIKK 519 + +N N I K Sbjct: 146 QELTPQNALNRITK 159 >SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 4196 Score = 27.1 bits (57), Expect = 2.5 Identities = 19/54 (35%), Positives = 30/54 (55%), Gaps = 2/54 (3%) Frame = +2 Query: 29 LKYSGEELKMELNERILKYLENSDKA--DTLKLCEEFNEEHQKIVGAVKSLEAL 184 L+ SG LK E + LK KA + LK + F+E+HQ+++GA+ + L Sbjct: 440 LRISGS-LKEEQSYYGLKNSIKQIKAFENKLKYIQSFHEKHQQLIGALSEVYGL 492 >SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1155 Score = 26.2 bits (55), Expect = 4.4 Identities = 18/57 (31%), Positives = 28/57 (49%) Frame = -3 Query: 527 KFPFFIAFLTFSDKLSIPFLISLRFSCTMSVIESTLRLTRGGPLDLSMYIHPDDMAL 357 KFP+ A TFSDK S F R++ T S + G +D +++ D +A+ Sbjct: 133 KFPYAYATFTFSDKPS--FTSIYRWNVTNSNVTIEHFYNVKGNVDSLFFLNNDSVAI 187 >SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 25.8 bits (54), Expect = 5.9 Identities = 12/31 (38%), Positives = 18/31 (58%) Frame = +2 Query: 41 GEELKMELNERILKYLENSDKADTLKLCEEF 133 G E+++ + E L+YLE DT+K EF Sbjct: 171 GTEIRLFMKEDQLQYLEEKTIKDTVKKHSEF 201 >SPBC83.03c |tas3||RITS complex subunit 3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 549 Score = 25.8 bits (54), Expect = 5.9 Identities = 15/55 (27%), Positives = 29/55 (52%) Frame = +1 Query: 355 SKAMSSGWIYIDKSSGPPLVKRKVDSITDIVQENLSEIRKGIDNLSENVRNAIKK 519 SK S ++DKSSG ++ +S +D + E+ S + + I ++R++ K Sbjct: 154 SKHRKSRNKFLDKSSGSMEIESWDNSTSDSIIESSSRLHESISLRENDIRSSDSK 208 >SPCC794.08 |||HEAT repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 3|||Manual Length = 798 Score = 25.4 bits (53), Expect = 7.8 Identities = 22/92 (23%), Positives = 42/92 (45%), Gaps = 5/92 (5%) Frame = +2 Query: 35 YSGEELKME-LNERILKYLENSDKADTLKLCEEFNEEH--QKIVGAVKS--LEALEMVIS 199 Y GE LK+ L K+L++ + L + H +K+ A + L+MV++ Sbjct: 596 YIGENLKITTLQSSGRKWLDSLSSSLPSSLLNRGSSTHSWEKLTNASTEEVMLPLDMVVN 655 Query: 200 EAVKITKWELTEEGELVAIMVATKPYYTEVYQ 295 E ++ + E+ L+ + P YT++ Q Sbjct: 656 ELLEKSPKVFPEDSRLLFAEQSRHPKYTDIIQ 687 >SPAC9.05 |mfh1||ATP-dependent DNA helicase Mfh1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 834 Score = 25.4 bits (53), Expect = 7.8 Identities = 10/40 (25%), Positives = 22/40 (55%) Frame = +1 Query: 415 KRKVDSITDIVQENLSEIRKGIDNLSENVRNAIKKGNFYK 534 K++VD + +++IR ++ E + + KGN+Y+ Sbjct: 265 KKEVDFFPVDLSAEITDIRDRFSSILEPMLQKLNKGNYYR 304 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,636,811 Number of Sequences: 5004 Number of extensions: 52897 Number of successful extensions: 186 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 178 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 185 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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