Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060832.seq
(688 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g39280.1 68417.m05563 phenylalanyl-tRNA synthetase, putative ... 46 2e-05
At5g24630.1 68418.m02909 expressed protein ; expression support... 33 0.18
At5g61920.1 68418.m07773 hypothetical protein 32 0.41
At1g49960.1 68414.m05606 xanthine/uracil permease family protein... 29 2.2
At4g30240.1 68417.m04300 expressed protein predicted protein, Ar... 29 3.8
At4g11850.1 68417.m01886 phospholipase D gamma 1 / PLD gamma 1 (... 28 5.0
At4g11830.2 68417.m01884 phospholipase D gamma 2 / PLD gamma 2 (... 28 5.0
At4g11830.1 68417.m01883 phospholipase D gamma 2 / PLD gamma 2 (... 28 5.0
At2g04660.1 68415.m00475 E3 ubiquitin ligase, putative E3, ubiqu... 28 5.0
At5g63220.1 68418.m07936 expressed protein contains Pfam PF04190... 28 6.7
At3g48390.1 68416.m05282 MA3 domain-containing protein similar t... 28 6.7
At5g62890.2 68418.m07892 permease, putative similar to permease ... 27 8.8
At5g62890.1 68418.m07891 permease, putative similar to permease ... 27 8.8
At5g49760.1 68418.m06163 leucine-rich repeat family protein / pr... 27 8.8
At1g60030.1 68414.m06763 xanthine/uracil permease family protein... 27 8.8
At1g56660.1 68414.m06516 expressed protein 27 8.8
>At4g39280.1 68417.m05563 phenylalanyl-tRNA synthetase, putative /
phenylalanine--tRNA ligase, putative similar to
SP|Q9Y285 Phenylalanyl-tRNA synthetase alpha chain (EC
6.1.1.20) (Phenylalanine- -tRNA ligase alpha chain)
(PheRS) {Homo sapiens}; contains Pfam profile PF01409:
tRNA synthetases class II core domain (F)
Length = 485
Score = 46.0 bits (104), Expect = 2e-05
Identities = 23/46 (50%), Positives = 29/46 (63%)
Frame = +3
Query: 546 KSFLLSKGPQFAITIKKLETDLTSDMLLSGSWKTLEFKPYNFDALG 683
K + + KGP +A KK+ TDLT + L + WK LEFK YNF A G
Sbjct: 162 KGYSVKKGPNYAPERKKVATDLTRENLQN--WKELEFKEYNFKAKG 205
Score = 44.0 bits (99), Expect = 9e-05
Identities = 21/63 (33%), Positives = 37/63 (58%), Gaps = 1/63 (1%)
Frame = +2
Query: 68 ERILKYLENSDK-ADTLKLCEEFNEEHQKIVGAVKSLEALEMVISEAVKITKWELTEEGE 244
E IL +L+N+++ D+ + EFN +H ++V +KSL + + +K W LT+EG+
Sbjct: 4 EAILGFLQNNEQITDSGQFSAEFNIDHNELVNVIKSLHGFRYIDVQDIKKETWILTDEGK 63
Query: 245 LVA 253
A
Sbjct: 64 KYA 66
>At5g24630.1 68418.m02909 expressed protein ; expression supported
by MPSS
Length = 531
Score = 33.1 bits (72), Expect = 0.18
Identities = 24/77 (31%), Positives = 36/77 (46%)
Frame = +2
Query: 20 PKKLKYSGEELKMELNERILKYLENSDKADTLKLCEEFNEEHQKIVGAVKSLEALEMVIS 199
PK+ + E LK E +R L + + DT + E QKI + S L +V+S
Sbjct: 281 PKEENCAQEILKTEGTQRFLYHADKDKDTDTDTIIAEEVTTDQKIKPSSGSSSRLPLVLS 340
Query: 200 EAVKITKWELTEEGELV 250
E V TK + EG+ +
Sbjct: 341 EKVNRTKVLVECEGDSI 357
>At5g61920.1 68418.m07773 hypothetical protein
Length = 238
Score = 31.9 bits (69), Expect = 0.41
Identities = 22/63 (34%), Positives = 35/63 (55%), Gaps = 2/63 (3%)
Frame = +2
Query: 23 KKLKYSGEELKM--ELNERILKYLENSDKADTLKLCEEFNEEHQKIVGAVKSLEALEMVI 196
+ L+ S +EL+ E ++R+ K E + KL + E +KI+GAVK++E L I
Sbjct: 170 ESLEASSQELERLKEEHQRLRKEFEEEKSGNVEKLAQLKGME-RKIIGAVKAIEKLRSEI 228
Query: 197 SEA 205
S A
Sbjct: 229 STA 231
>At1g49960.1 68414.m05606 xanthine/uracil permease family protein
contains Pfam profile: PF00860 permease family
Length = 526
Score = 29.5 bits (63), Expect = 2.2
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = -1
Query: 226 LPFCNLNSFRNNHFKSFQTF 167
L FCNLNSFRN F F
Sbjct: 408 LQFCNLNSFRNKFILGFSIF 427
>At4g30240.1 68417.m04300 expressed protein predicted protein,
Arabidopsis thaliana
Length = 300
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 223 GVN*RRRTRCYNGSHEAVLYRSIPDSGVPQSEAMK 327
G+N RR RCY S + V+ I D G P+S K
Sbjct: 168 GINGRREGRCYGDSPDCVI--DIDDIGSPESADKK 200
>At4g11850.1 68417.m01886 phospholipase D gamma 1 / PLD gamma 1
(PLDGAMMA1) identical to phospholipase D gamma 1
SP:Q9T053 from [Arabidopsis thaliana]
Length = 858
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/52 (30%), Positives = 31/52 (59%), Gaps = 3/52 (5%)
Frame = +2
Query: 32 KYSGEELKMELNERILKY---LENSDKADTLKLCEEFNEEHQKIVGAVKSLE 178
+Y+ EE+ E++ +LKY ++ + K +L CE F + KI+G+ +L+
Sbjct: 803 QYAAEEVT-EMSGHLLKYPVQVDRTGKVSSLPGCETFPDLGGKIIGSFLALQ 853
>At4g11830.2 68417.m01884 phospholipase D gamma 2 / PLD gamma 2
(PLDGAMMA2) identical to SP|Q9T051 Phospholipase D gamma
2 (EC 3.1.4.4) (AtPLDgamma2) (PLD gamma 2) [Arabidopsis
thaliana]
Length = 856
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/52 (30%), Positives = 31/52 (59%), Gaps = 3/52 (5%)
Frame = +2
Query: 32 KYSGEELKMELNERILKY---LENSDKADTLKLCEEFNEEHQKIVGAVKSLE 178
+Y+ EE+ E++ +LKY ++ + K +L CE F + KI+G+ +L+
Sbjct: 801 QYAAEEVT-EMSGHLLKYPVQVDKTGKVSSLPGCETFPDLGGKIIGSFLTLQ 851
>At4g11830.1 68417.m01883 phospholipase D gamma 2 / PLD gamma 2
(PLDGAMMA2) identical to SP|Q9T051 Phospholipase D gamma
2 (EC 3.1.4.4) (AtPLDgamma2) (PLD gamma 2) [Arabidopsis
thaliana]
Length = 824
Score = 28.3 bits (60), Expect = 5.0
Identities = 16/52 (30%), Positives = 31/52 (59%), Gaps = 3/52 (5%)
Frame = +2
Query: 32 KYSGEELKMELNERILKY---LENSDKADTLKLCEEFNEEHQKIVGAVKSLE 178
+Y+ EE+ E++ +LKY ++ + K +L CE F + KI+G+ +L+
Sbjct: 769 QYAAEEVT-EMSGHLLKYPVQVDKTGKVSSLPGCETFPDLGGKIIGSFLTLQ 819
>At2g04660.1 68415.m00475 E3 ubiquitin ligase, putative E3,
ubiquitin ligase; contains similarity to
anaphase-promoting complex subunit 2 GI:6180009 from
[Homo sapiens]
Length = 865
Score = 28.3 bits (60), Expect = 5.0
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +2
Query: 59 ELNERILKYLENSDKADTLKLCEEFNEEHQKIV 157
+L E I+ Y E+S + LK C E+ +H K+V
Sbjct: 367 KLFEIIVDYPESSPAIEDLKQCLEYTGQHSKLV 399
>At5g63220.1 68418.m07936 expressed protein contains Pfam PF04190:
Protein of unknown function (DUF410)
Length = 324
Score = 27.9 bits (59), Expect = 6.7
Identities = 15/38 (39%), Positives = 21/38 (55%)
Frame = +1
Query: 403 PPLVKRKVDSITDIVQENLSEIRKGIDNLSENVRNAIK 516
P LV D +QE+L E R ++NL+ +R AIK
Sbjct: 112 PHLVDVSDDEDVQNLQESLGEARSRVENLTSFLRAAIK 149
>At3g48390.1 68416.m05282 MA3 domain-containing protein similar to
programmed cell death 4 protein [Gallus gallus]
GI:12958564; contains Pfam profile PF02847: MA3 domain
Length = 633
Score = 27.9 bits (59), Expect = 6.7
Identities = 24/78 (30%), Positives = 40/78 (51%), Gaps = 4/78 (5%)
Frame = +2
Query: 44 EELKMELNERILKYLENSDKADTLKLCEEFNEE--HQKIVGAVKSLEALEMVISE--AVK 211
EE K +++E + +Y+EN D + + E H +IV + L +E SE +K
Sbjct: 219 EETKRKISEFLNEYVENGDTREACRCIRELGVSFFHHEIVKSGLVL-VMESRTSEPLILK 277
Query: 212 ITKWELTEEGELVAIMVA 265
+ K E TEEG + + +A
Sbjct: 278 LLK-EATEEGLISSSQMA 294
>At5g62890.2 68418.m07892 permease, putative similar to permease 1
[Zea mays] GI:7844006; contains Pfam profile: PF00860
Xanthine/uracil permeases family; identical to cDNA
putative permease 1 (MQB2.21) GI:21326024
Length = 532
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = -1
Query: 226 LPFCNLNSFRNNHFKSFQTF 167
L FCNLNSFR F F
Sbjct: 414 LQFCNLNSFRTKFILGFSVF 433
>At5g62890.1 68418.m07891 permease, putative similar to permease 1
[Zea mays] GI:7844006; contains Pfam profile: PF00860
Xanthine/uracil permeases family; identical to cDNA
putative permease 1 (MQB2.21) GI:21326024
Length = 476
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = -1
Query: 226 LPFCNLNSFRNNHFKSFQTF 167
L FCNLNSFR F F
Sbjct: 414 LQFCNLNSFRTKFILGFSVF 433
>At5g49760.1 68418.m06163 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 953
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/75 (17%), Positives = 36/75 (48%), Gaps = 4/75 (5%)
Frame = +1
Query: 250 CYNGSHEAVLYRSIPDSGVPQS----EAMKMVPNAKVGFSKAMSSGWIYIDKSSGPPLVK 417
C++ + + ++Y I + + S +++ ++ + G Y+ + + PP++
Sbjct: 694 CFDRNEQMLVYEYISNGSLKDSLSGKSGIRLDWTRRLKIALGSGKGLAYLHELADPPIIH 753
Query: 418 RKVDSITDIVQENLS 462
R + S ++ ENL+
Sbjct: 754 RDIKSNNILLDENLT 768
>At1g60030.1 68414.m06763 xanthine/uracil permease family protein
contains Pfam profile: PF00860 permease family
Length = 538
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = -1
Query: 226 LPFCNLNSFRNNHFKSFQTF 167
L FCNLNSFR F F
Sbjct: 420 LQFCNLNSFRTKFILGFSVF 439
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +2
Query: 20 PKKLKYSGEELKMELNERILKYLENSDKADTLKLCEEFNEEHQK 151
PKK K EE K ++++ E +K D K EE +EH +
Sbjct: 194 PKKEKKQKEESKSNEDKKVKGKKEKGEKGDLEKEDEEKKKEHDE 237
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,386,356
Number of Sequences: 28952
Number of extensions: 262965
Number of successful extensions: 837
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 819
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 836
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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