BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060822.seq (695 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 26 4.5 SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 26 4.5 SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha... 25 7.8 SPBP8B7.07c |set6||histone lysine methyltransferase Set6 |Schizo... 25 7.8 SPAC30D11.06c |||DUF300 family protein|Schizosaccharomyces pombe... 25 7.8 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 26.2 bits (55), Expect = 4.5 Identities = 8/23 (34%), Positives = 15/23 (65%) Frame = -1 Query: 185 MRNGYCSSQLGSRLFSRQCYAHG 117 ++ G C +Q+GS+ + + C HG Sbjct: 8 LQAGQCGNQIGSQFWQQLCLEHG 30 >SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1583 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/31 (35%), Positives = 16/31 (51%) Frame = +1 Query: 514 STEAKKEDATTPKSELAKSTEAPTHMEPTVQ 606 S + K+D TTPK L + T +P +Q Sbjct: 179 SPKKSKDDITTPKKRLMEDTYSPRESPSKIQ 209 >SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccharomyces pombe|chr 3|||Manual Length = 688 Score = 25.4 bits (53), Expect = 7.8 Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 4/49 (8%) Frame = +1 Query: 508 SSSTEAKKEDATTPKSELAKSTEAPTHMEPTVQGKSI*WT----KLCWW 642 S +AK E A ++ STE+PT+ T QG S+ + K+C W Sbjct: 276 SMMAKAKAEAALPVGTKEMVSTESPTYKALTKQGYSVVGSHSGVKICRW 324 >SPBP8B7.07c |set6||histone lysine methyltransferase Set6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 483 Score = 25.4 bits (53), Expect = 7.8 Identities = 12/35 (34%), Positives = 22/35 (62%), Gaps = 1/35 (2%) Frame = +1 Query: 133 CRLN-SLEPNWELQYPFLMISLKLQVNLKKTQQQF 234 CRLN S +PN ++ + ++ L + ++KK +Q F Sbjct: 190 CRLNHSCDPNCQIIFDGAIVQLVSKRDIKKDEQLF 224 >SPAC30D11.06c |||DUF300 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 426 Score = 25.4 bits (53), Expect = 7.8 Identities = 15/51 (29%), Positives = 27/51 (52%) Frame = +1 Query: 46 CLFLLKISFISK*RSWRSSVY*NLPCA*HCRLNSLEPNWELQYPFLMISLK 198 C F L I ++ R+ S++ +LP LN L+ + +L P+ +S+K Sbjct: 81 CFFCLLIDYLGGERAAVISLHGHLPRPRLWPLNYLQDDIDLSDPYTFLSIK 131 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,430,181 Number of Sequences: 5004 Number of extensions: 41195 Number of successful extensions: 124 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 120 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 124 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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