BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060821.seq (630 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 compon... 46 5e-06 SPCC1259.09c |||pyruvate dehydrogenase protein x component|Schiz... 32 0.079 SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 1.7 SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce... 27 2.2 SPBC4.02c |||conserved fungal protein|Schizosaccharomyces pombe|... 26 3.9 SPBC19F8.02 |||nuclear distribution protein NUDC |Schizosaccharo... 26 3.9 SPCC1259.08 |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.2 SPBP8B7.26 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.2 SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 26 5.2 SPCC1840.09 |||NAD dependent epimerase/dehydratase family protei... 26 5.2 SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosa... 25 6.8 SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi... 25 9.0 SPBC83.07 |jmj3||Lid2 complex subunit Jmj3|Schizosaccharomyces p... 25 9.0 >SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 component of oxoglutarate dehydrogenase complex |Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 46.0 bits (104), Expect = 5e-06 Identities = 20/47 (42%), Positives = 29/47 (61%) Frame = +1 Query: 379 ESGRQRXADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVKLDK 519 + G DE + +ETDK PV AP GV+KE VK+G+T+ +D+ Sbjct: 65 QPGEYVNKDEEIASVETDKIDAPVTAPDAGVLKEQLVKEGDTITIDQ 111 >SPCC1259.09c |||pyruvate dehydrogenase protein x component|Schizosaccharomyces pombe|chr 3|||Manual Length = 456 Score = 31.9 bits (69), Expect = 0.079 Identities = 11/48 (22%), Positives = 28/48 (58%) Frame = +1 Query: 379 ESGRQRXADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVKLDKS 522 + G + +++LE+ETDK + V +G++ ++ ++ G + + K+ Sbjct: 58 KEGDSFKSGDILLEVETDKATMDVEVQDNGILAKVLIEKGSNIPVGKN 105 >SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 27.5 bits (58), Expect = 1.7 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +2 Query: 197 NNSNAQNTCTLTRNQTEPTGALVAHNQVAS 286 NNS N+ T++ NQ PT A A +++ S Sbjct: 391 NNSEWNNSMTMSSNQETPTAASCAVSRIGS 420 >SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 818 Score = 27.1 bits (57), Expect = 2.2 Identities = 15/58 (25%), Positives = 25/58 (43%) Frame = +2 Query: 182 HPLQVNNSNAQNTCTLTRNQTEPTGALVAHNQVASIHFTNPLLVEQDVTTPSFPDSVS 355 HP NN+++ T ++ E A + N V S PL V+ +V + +S Sbjct: 351 HPSTNNNASSYQNFNETSDEGEDNDATIRANNVRSSFLEAPLPVQPNVQAETVTPKIS 408 >SPBC4.02c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 456 Score = 26.2 bits (55), Expect = 3.9 Identities = 9/31 (29%), Positives = 16/31 (51%) Frame = -1 Query: 273 LWATRAPVGSVWLRVRVQVFWAFELLT*SGW 181 L+ T P ++W +++ AF +L GW Sbjct: 56 LFQTPLPNANIWSNQAIRILMAFNILANGGW 86 >SPBC19F8.02 |||nuclear distribution protein NUDC |Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 26.2 bits (55), Expect = 3.9 Identities = 11/21 (52%), Positives = 13/21 (61%) Frame = +3 Query: 228 LHATKLNQPELWSPTIK*HPS 290 +H K N+ E WS IK HPS Sbjct: 82 IHLEKSNKMEWWSCVIKGHPS 102 >SPCC1259.08 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 394 Score = 25.8 bits (54), Expect = 5.2 Identities = 21/68 (30%), Positives = 34/68 (50%) Frame = +2 Query: 305 LLVEQDVTTPSFPDSVSEGDVKLDKKVGDSVXPMKWSWKSRRTKPPFRSWRPAMVSSRSC 484 +++ QD+ P+FPDS E D VG S+ + +K+ + K ++S A VSS Sbjct: 318 IMLPQDID-PTFPDSEPEDDGHASSTVG-SLSKEEARFKA-KAKWNYKSSFSAHVSSEVL 374 Query: 485 TSKTEKPL 508 + PL Sbjct: 375 RNSKSPPL 382 >SPBP8B7.26 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 25.8 bits (54), Expect = 5.2 Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 1/36 (2%) Frame = +1 Query: 139 ARSTSRRCTGVRASPSASSQQLKRPKYLHP-YTQPN 243 A +S T ASP+ +Q +P Y P Y+QP+ Sbjct: 71 AGGSSTGSTPRTASPAVGNQDYSKPSYSQPSYSQPS 106 >SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces pombe|chr 2|||Manual Length = 2493 Score = 25.8 bits (54), Expect = 5.2 Identities = 20/84 (23%), Positives = 36/84 (42%) Frame = +2 Query: 188 LQVNNSNAQNTCTLTRNQTEPTGALVAHNQVASIHFTNPLLVEQDVTTPSFPDSVSEGDV 367 L + S +++ L RN + + AL + +H L + + TP +PD Sbjct: 621 LPFDPSTSRDASVLLRNLSAKSSALEKDTRRVVLHALLGLTITR--FTPLWPDLSRTAAS 678 Query: 368 KLDKKVGDSVXPMKWSWKSRRTKP 439 + K+V D + +SW S + P Sbjct: 679 IVTKEVEDEFLAIIYSWLSLPSPP 702 >SPCC1840.09 |||NAD dependent epimerase/dehydratase family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 276 Score = 25.8 bits (54), Expect = 5.2 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = -2 Query: 485 YNSLMTPWPGAMTGMAVLSVSISRTTSSAXRCL 387 YN P+ GA+ + +S SI+R TS A L Sbjct: 197 YNFNDRPFTGALASLFTVSSSINRATSGALNFL 229 >SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 660 Score = 25.4 bits (53), Expect = 6.8 Identities = 23/100 (23%), Positives = 40/100 (40%), Gaps = 1/100 (1%) Frame = +2 Query: 59 VKSIITTKRQRQNKR*VRSISENATTLLEAHPDAVPAS-GPVHPLQVNNSNAQNTCTLTR 235 V + +T + K+ + + +++ LE P + P H L N+ NAQ + Sbjct: 67 VNGVNSTTIKENTKKEISNPNDSTRLPLEQIPGSNNLFLDPRHQLGDNSQNAQRHHEPSF 126 Query: 236 NQTEPTGALVAHNQVASIHFTNPLLVEQDVTTPSFPDSVS 355 N + + + VA +P +PSFP S S Sbjct: 127 NSEKASYTSTPYKNVAPKVIDSPSARHMHSNSPSFPPSQS 166 >SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schizosaccharomyces pombe|chr 2|||Manual Length = 965 Score = 25.0 bits (52), Expect = 9.0 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = +2 Query: 119 SENATTLLEAHPDAVPASGPVHP 187 S+ A L+E + DAVPAS P Sbjct: 924 SDKAPDLIELNRDAVPASADTEP 946 >SPBC83.07 |jmj3||Lid2 complex subunit Jmj3|Schizosaccharomyces pombe|chr 2|||Manual Length = 752 Score = 25.0 bits (52), Expect = 9.0 Identities = 11/52 (21%), Positives = 24/52 (46%) Frame = +2 Query: 176 PVHPLQVNNSNAQNTCTLTRNQTEPTGALVAHNQVASIHFTNPLLVEQDVTT 331 P+H NNS +Q + P+ + H ++ HF + E+++++ Sbjct: 382 PIHSSVDNNSFSQRDFDSPNSINPPSPLMSNHESASTEHFNSTTTTEKELSS 433 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,470,389 Number of Sequences: 5004 Number of extensions: 51960 Number of successful extensions: 155 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 152 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 155 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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