Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060821.seq
         (630 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ...    46   2e-05
At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein ...    45   4e-05
At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein ...    45   4e-05
At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein ...    45   4e-05
At2g31130.1 68415.m03801 expressed protein                             29   1.9  
At5g56320.1 68418.m07029 expansin, putative (EXP14) similar to a...    29   3.4  
At4g25880.2 68417.m03722 pumilio/Puf RNA-binding domain-containi...    28   5.9  
At4g25880.1 68417.m03721 pumilio/Puf RNA-binding domain-containi...    28   5.9  

>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
           similar to SP|Q01205 Dihydrolipoamide
           succinyltransferase component of 2-oxoglutarate
           dehydrogenase complex, mitochondrial precursor (EC
           2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
           PF00198: 2-oxo acid dehydrogenases acyltransferase
           (catalytic domain), PF00364: Biotin-requiring enzyme
          Length = 464

 Score = 46.4 bits (105), Expect = 2e-05
 Identities = 20/42 (47%), Positives = 29/42 (69%)
 Frame = +1

Query: 385 GRQRXADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVK 510
           G +  ADE + +IETDK  I + +P  GVI+E  VK+G+TV+
Sbjct: 118 GDRVEADEAIAQIETDKVTIDIASPASGVIQEFLVKEGDTVE 159


>At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein
           similar to SP|P36957 Dihydrolipoamide
           succinyltransferase component of 2-oxoglutarate
           dehydrogenase complex, mitochondrial precursor (EC
           2.3.1.61) {Homo sapiens}; contains Pfam profiles
           PF00198: 2-oxo acid dehydrogenases acyltransferase
           (catalytic domain), PF00364: Biotin-requiring enzyme
          Length = 365

 Score = 45.2 bits (102), Expect = 4e-05
 Identities = 19/42 (45%), Positives = 28/42 (66%)
 Frame = +1

Query: 385 GRQRXADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVK 510
           G +  ADE + +IETDK  I + +P  GVI+E  V +G+TV+
Sbjct: 18  GERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNEGDTVE 59


>At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein
           similar to SP|P36957 Dihydrolipoamide
           succinyltransferase component of 2-oxoglutarate
           dehydrogenase complex, mitochondrial precursor (EC
           2.3.1.61) {Homo sapiens}; contains Pfam profiles
           PF00198: 2-oxo acid dehydrogenases acyltransferase
           (catalytic domain), PF00364: Biotin-requiring enzyme
          Length = 463

 Score = 45.2 bits (102), Expect = 4e-05
 Identities = 19/42 (45%), Positives = 28/42 (66%)
 Frame = +1

Query: 385 GRQRXADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVK 510
           G +  ADE + +IETDK  I + +P  GVI+E  V +G+TV+
Sbjct: 116 GERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNEGDTVE 157


>At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein
           similar to SP|P36957 Dihydrolipoamide
           succinyltransferase component of 2-oxoglutarate
           dehydrogenase complex, mitochondrial precursor (EC
           2.3.1.61) {Homo sapiens}; contains Pfam profiles
           PF00198: 2-oxo acid dehydrogenases acyltransferase
           (catalytic domain), PF00364: Biotin-requiring enzyme
          Length = 464

 Score = 45.2 bits (102), Expect = 4e-05
 Identities = 19/42 (45%), Positives = 28/42 (66%)
 Frame = +1

Query: 385 GRQRXADEVVLEIETDKTAIPVMAPGHGVIKELYVKDGETVK 510
           G +  ADE + +IETDK  I + +P  GVI+E  V +G+TV+
Sbjct: 117 GERVQADEAIAQIETDKVTIDIASPASGVIQEFLVNEGDTVE 158


>At2g31130.1 68415.m03801 expressed protein
          Length = 419

 Score = 29.5 bits (63), Expect = 1.9
 Identities = 22/98 (22%), Positives = 40/98 (40%)
 Frame = +2

Query: 215 NTCTLTRNQTEPTGALVAHNQVASIHFTNPLLVEQDVTTPSFPDSVSEGDVKLDKKVGDS 394
           N  T + + +EP   ++ H    S+   + +LV++D     FPD +     K  KK+ D+
Sbjct: 309 NVETSSSSVSEPKSEILQHLSGRSVE-ESCILVDRDEFHSVFPDKMENDKHKPYKKIRDA 367

Query: 395 VXPMKWSWKSRRTKPPFRSWRPAMVSSRSCTSKTEKPL 508
           +       + +  K   R W    V +        KP+
Sbjct: 368 ISSRMKQNREKEYKRLARQWYAEDVENGRECGDNPKPI 405


>At5g56320.1 68418.m07029 expansin, putative (EXP14) similar to
           alpha-expansin 3 GI:6942322 from [Triphysaria
           versicolor]; alpha-expansin gene family, PMID:11641069
          Length = 255

 Score = 28.7 bits (61), Expect = 3.4
 Identities = 17/54 (31%), Positives = 27/54 (50%)
 Frame = +3

Query: 87  SVKIKGKYVQ*AKMLRRCSKHIQTLYRRQGQSIRFKSTTQTPKILAPLHATKLN 248
           SV IKG   +   M R   ++ Q+  +  GQ++ FK TT   + +   +AT  N
Sbjct: 186 SVSIKGTNTRWQSMSRNWGQNWQSNAKLDGQALSFKVTTSDGRTVISNNATPRN 239


>At4g25880.2 68417.m03722 pumilio/Puf RNA-binding domain-containing
           protein contains Pfam profile:PF00806 Pumilio-family RNA
           binding domains
          Length = 852

 Score = 27.9 bits (59), Expect = 5.9
 Identities = 12/37 (32%), Positives = 19/37 (51%)
 Frame = +3

Query: 195 STTQTPKILAPLHATKLNQPELWSPTIK*HPSTSRIP 305
           + TQT  + +P H   +  P ++SP     P T+ IP
Sbjct: 316 TATQTAYMTSPAHVYNMQSPAVYSPQYGYGPYTNMIP 352


>At4g25880.1 68417.m03721 pumilio/Puf RNA-binding domain-containing
           protein contains Pfam profile:PF00806 Pumilio-family RNA
           binding domains
          Length = 861

 Score = 27.9 bits (59), Expect = 5.9
 Identities = 12/37 (32%), Positives = 19/37 (51%)
 Frame = +3

Query: 195 STTQTPKILAPLHATKLNQPELWSPTIK*HPSTSRIP 305
           + TQT  + +P H   +  P ++SP     P T+ IP
Sbjct: 316 TATQTAYMTSPAHVYNMQSPAVYSPQYGYGPYTNMIP 352


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,160,097
Number of Sequences: 28952
Number of extensions: 282178
Number of successful extensions: 825
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 809
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 825
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)