Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060813.seq
(631 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 134 1e-32
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 134 1e-32
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 134 1e-32
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 81 2e-16
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 80 3e-16
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 57 2e-09
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.006
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.006
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 35 0.011
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 34 0.015
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 34 0.019
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 31 0.14
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.18
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.24
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.42
SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual 29 0.73
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 3.0
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 26 3.9
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 9.0
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 134 bits (324), Expect = 1e-32
Identities = 61/68 (89%), Positives = 64/68 (94%)
Frame = +2
Query: 299 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 478
GITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCAVLI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISK 129
Query: 479 NGQTREHA 502
+GQTREHA
Sbjct: 130 DGQTREHA 137
Score = 109 bits (261), Expect = 4e-25
Identities = 49/53 (92%), Positives = 51/53 (96%)
Frame = +1
Query: 94 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS 252
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGS
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
Score = 47.6 bits (108), Expect = 1e-06
Identities = 25/39 (64%), Positives = 30/39 (76%)
Frame = +1
Query: 502 LAPFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIQKEVSH 618
L +TLGVKQLIV VNKMD+T +S+ RFEEI KE S+
Sbjct: 138 LLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSN 174
Score = 36.3 bits (80), Expect = 0.004
Identities = 18/33 (54%), Positives = 22/33 (66%)
Frame = +3
Query: 198 QTYHREVREGGPGNG*RILKYAWVLDKLKAERE 296
+ + +E E G G+ KYAWVLDKLKAERE
Sbjct: 40 EKFEKEATELGKGS----FKYAWVLDKLKAERE 68
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 134 bits (323), Expect = 1e-32
Identities = 60/68 (88%), Positives = 64/68 (94%)
Frame = +2
Query: 299 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 478
GITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISK 129
Query: 479 NGQTREHA 502
+GQTREHA
Sbjct: 130 DGQTREHA 137
Score = 109 bits (261), Expect = 4e-25
Identities = 49/53 (92%), Positives = 51/53 (96%)
Frame = +1
Query: 94 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS 252
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGS
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
Score = 47.6 bits (108), Expect = 1e-06
Identities = 25/39 (64%), Positives = 30/39 (76%)
Frame = +1
Query: 502 LAPFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIQKEVSH 618
L +TLGVKQLIV VNKMD+T +S+ RFEEI KE S+
Sbjct: 138 LLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSN 174
Score = 36.3 bits (80), Expect = 0.004
Identities = 18/33 (54%), Positives = 22/33 (66%)
Frame = +3
Query: 198 QTYHREVREGGPGNG*RILKYAWVLDKLKAERE 296
+ + +E E G G+ KYAWVLDKLKAERE
Sbjct: 40 EKFEKEATELGKGS----FKYAWVLDKLKAERE 68
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 134 bits (323), Expect = 1e-32
Identities = 60/68 (88%), Positives = 64/68 (94%)
Frame = +2
Query: 299 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 478
GITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISK 129
Query: 479 NGQTREHA 502
+GQTREHA
Sbjct: 130 DGQTREHA 137
Score = 109 bits (261), Expect = 4e-25
Identities = 49/53 (92%), Positives = 51/53 (96%)
Frame = +1
Query: 94 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS 252
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGS
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
Score = 47.6 bits (108), Expect = 1e-06
Identities = 25/39 (64%), Positives = 30/39 (76%)
Frame = +1
Query: 502 LAPFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIQKEVSH 618
L +TLGVKQLIV VNKMD+T +S+ RFEEI KE S+
Sbjct: 138 LLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSN 174
Score = 36.3 bits (80), Expect = 0.004
Identities = 18/33 (54%), Positives = 22/33 (66%)
Frame = +3
Query: 198 QTYHREVREGGPGNG*RILKYAWVLDKLKAERE 296
+ + +E E G G+ KYAWVLDKLKAERE
Sbjct: 40 EKFEKEATELGKGS----FKYAWVLDKLKAERE 68
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 80.6 bits (190), Expect = 2e-16
Identities = 38/68 (55%), Positives = 45/68 (66%)
Frame = +2
Query: 299 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 478
G+T+D+A FE+ K I DAPGHRDFI MI G S AD AVL+V + FE G +
Sbjct: 240 GVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLE 299
Query: 479 NGQTREHA 502
NGQTREHA
Sbjct: 300 NGQTREHA 307
Score = 55.6 bits (128), Expect = 6e-09
Identities = 24/56 (42%), Positives = 36/56 (64%)
Frame = +1
Query: 91 EMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSLN 258
E K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGS +
Sbjct: 170 EQSNPKPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFS 225
Score = 31.9 bits (69), Expect = 0.079
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = +1
Query: 517 LGVKQLIVGVNKMDSTEPPYSEPRFEEIQKEVS 615
LG+ +++V VNK+D +SE RF+EI+ VS
Sbjct: 313 LGISEIVVSVNKLDLMS--WSEDRFQEIKNIVS 343
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 79.8 bits (188), Expect = 3e-16
Identities = 34/68 (50%), Positives = 47/68 (69%)
Frame = +2
Query: 299 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 478
G T+++ FET +++DAPGH+ ++ NMI G SQAD VL+++A GEFEAG +
Sbjct: 301 GKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFER 360
Query: 479 NGQTREHA 502
GQTREHA
Sbjct: 361 GGQTREHA 368
Score = 67.3 bits (157), Expect = 2e-12
Identities = 29/49 (59%), Positives = 39/49 (79%)
Frame = +1
Query: 106 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS 252
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKES 284
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 56.8 bits (131), Expect = 2e-09
Identities = 29/67 (43%), Positives = 41/67 (61%)
Frame = +2
Query: 299 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 478
GITI A ++ET+ + +D PGH D+IKNMITG + D A+++V+A G+
Sbjct: 101 GITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP----- 155
Query: 479 NGQTREH 499
QTREH
Sbjct: 156 --QTREH 160
Score = 35.1 bits (77), Expect = 0.008
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +1
Query: 100 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 186
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
Score = 28.3 bits (60), Expect = 0.97
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +1
Query: 517 LGVKQLIVGVNKMDSTEPPYSE 582
+GVKQ++V +NK+D EP E
Sbjct: 167 VGVKQIVVYINKVDMVEPDMIE 188
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.006
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 94 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 189
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 344 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 439
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.006
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 94 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 189
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 344 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 439
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 34.7 bits (76), Expect = 0.011
Identities = 18/52 (34%), Positives = 24/52 (46%)
Frame = +2
Query: 299 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 454
GITI+ A F + +ID PGH DF + + D AV I+ G
Sbjct: 78 GITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAG 129
Score = 28.7 bits (61), Expect = 0.73
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +1
Query: 118 NIVVIGHVDSGKSTTTGHLIYKCG 189
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 34.3 bits (75), Expect = 0.015
Identities = 21/57 (36%), Positives = 29/57 (50%)
Frame = +2
Query: 329 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 499
FE S Y + IID PGH DF + D AVL++ A +G I+ + Q R +
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
Score = 29.1 bits (62), Expect = 0.55
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 118 NIVVIGHVDSGKSTTTGHLIYKCGGI 195
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 33.9 bits (74), Expect = 0.019
Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +2
Query: 299 GITIDIALWK--FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 454
GIT I + F+ ++T +D PGH F G + AD VL+VA G
Sbjct: 203 GITQKIGAFTVPFDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 31.1 bits (67), Expect = 0.14
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +2
Query: 347 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 442
+V+ +D PGH + M+ G + D A+L++A
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIA 140
Score = 25.0 bits (52), Expect = 9.0
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +1
Query: 88 PEMGKEKTHINIVVIGHVDSGKST 159
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.7 bits (66), Expect = 0.18
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +2
Query: 320 LWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 454
++ + Y + +ID PGH DF ++ + + +L+V A G
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQG 160
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 30.3 bits (65), Expect = 0.24
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 344 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 454
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.42
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 356 IIDAPGHRDFIKNMITGTSQADCAVLIV 439
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual
Length = 815
Score = 28.7 bits (61), Expect = 0.73
Identities = 21/80 (26%), Positives = 34/80 (42%), Gaps = 4/80 (5%)
Frame = +1
Query: 100 KEKTHINIVVIGHVDS----GKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSLNMLG 267
KE ++N+V D S++T + + K GI + +EK + G N L
Sbjct: 288 KENVNLNLVKTPKYDDLTKMNLSSSTANTLIK--GIQSYNFQNYEKAYSLLAAGVTNSLL 345
Query: 268 YWTN*RLSVXXYHNRYCSLE 327
WTN +S Y C ++
Sbjct: 346 EWTNTSVSDKEYLRIQCFIQ 365
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 26.6 bits (56), Expect = 3.0
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +2
Query: 359 IDAPGHRDFIKNMITGTSQADCAVLIV 439
ID PGH DF+ + + +D VL+V
Sbjct: 214 IDTPGHVDFVDEVAAPMAISDGVVLVV 240
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 26.2 bits (55), Expect = 3.9
Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Frame = +2
Query: 302 ITIDIALWKFETSKYYV--TIIDAPGHRDFIKN 394
+ I+I + E +++ T+ID PG DFI N
Sbjct: 71 VEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 9.0
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -1
Query: 235 PGPPSRTSRWYVCQYHHIC 179
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,578,511
Number of Sequences: 5004
Number of extensions: 50982
Number of successful extensions: 185
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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