Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060813.seq
(631 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 146 2e-37
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 146 2e-37
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 142 3e-36
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 138 4e-35
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.002
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 25 0.80
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 23 1.9
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.4
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.2
DQ869051-1|ABJ09598.1| 581|Apis mellifera pyrokinin-like recept... 21 7.5
DQ855484-1|ABH88171.1| 130|Apis mellifera chemosensory protein ... 21 9.9
AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr... 21 9.9
AJ973401-1|CAJ01448.1| 130|Apis mellifera hypothetical protein ... 21 9.9
AF481963-1|AAN59784.1| 130|Apis mellifera antennal-specific pro... 21 9.9
AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase pro... 21 9.9
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 146 bits (353), Expect = 2e-37
Identities = 68/68 (100%), Positives = 68/68 (100%)
Frame = +2
Query: 299 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 478
GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK
Sbjct: 13 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 72
Query: 479 NGQTREHA 502
NGQTREHA
Sbjct: 73 NGQTREHA 80
Score = 71.7 bits (168), Expect = 5e-15
Identities = 34/38 (89%), Positives = 35/38 (92%)
Frame = +1
Query: 502 LAPFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIQKEVS 615
L FTLGVKQLIVGVNKMDSTEPPYSE RFEEI+KEVS
Sbjct: 81 LLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVS 118
Score = 27.5 bits (58), Expect = 0.11
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +3
Query: 264 WVLDKLKAERE 296
WVLDKLKAERE
Sbjct: 1 WVLDKLKAERE 11
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 146 bits (353), Expect = 2e-37
Identities = 68/68 (100%), Positives = 68/68 (100%)
Frame = +2
Query: 299 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 478
GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK
Sbjct: 70 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 129
Query: 479 NGQTREHA 502
NGQTREHA
Sbjct: 130 NGQTREHA 137
Score = 113 bits (272), Expect = 1e-27
Identities = 52/53 (98%), Positives = 52/53 (98%)
Frame = +1
Query: 94 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS 252
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS 53
Score = 71.7 bits (168), Expect = 5e-15
Identities = 34/38 (89%), Positives = 35/38 (92%)
Frame = +1
Query: 502 LAPFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIQKEVS 615
L FTLGVKQLIVGVNKMDSTEPPYSE RFEEI+KEVS
Sbjct: 138 LLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVS 175
Score = 37.5 bits (83), Expect = 1e-04
Identities = 18/33 (54%), Positives = 23/33 (69%)
Frame = +3
Query: 198 QTYHREVREGGPGNG*RILKYAWVLDKLKAERE 296
+ + +E +E G G+ KYAWVLDKLKAERE
Sbjct: 40 EKFEKEAQEMGKGS----FKYAWVLDKLKAERE 68
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 142 bits (344), Expect = 3e-36
Identities = 66/68 (97%), Positives = 67/68 (98%)
Frame = +2
Query: 299 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 478
GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISK
Sbjct: 70 GITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISK 129
Query: 479 NGQTREHA 502
NGQTREHA
Sbjct: 130 NGQTREHA 137
Score = 113 bits (272), Expect = 1e-27
Identities = 52/53 (98%), Positives = 52/53 (98%)
Frame = +1
Query: 94 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS 252
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGS 53
Score = 68.5 bits (160), Expect = 5e-14
Identities = 32/38 (84%), Positives = 34/38 (89%)
Frame = +1
Query: 502 LAPFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIQKEVS 615
L FTLGVKQLIVGVNKMD T+PPYSE RFEEI+KEVS
Sbjct: 138 LLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVS 175
Score = 37.5 bits (83), Expect = 1e-04
Identities = 18/33 (54%), Positives = 23/33 (69%)
Frame = +3
Query: 198 QTYHREVREGGPGNG*RILKYAWVLDKLKAERE 296
+ + +E +E G G+ KYAWVLDKLKAERE
Sbjct: 40 EKFEKEAQEMGKGS----FKYAWVLDKLKAERE 68
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 138 bits (334), Expect = 4e-35
Identities = 64/64 (100%), Positives = 64/64 (100%)
Frame = +2
Query: 311 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 490
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 491 REHA 502
REHA
Sbjct: 61 REHA 64
Score = 71.7 bits (168), Expect = 5e-15
Identities = 34/38 (89%), Positives = 35/38 (92%)
Frame = +1
Query: 502 LAPFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIQKEVS 615
L FTLGVKQLIVGVNKMDSTEPPYSE RFEEI+KEVS
Sbjct: 65 LLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVS 102
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.002
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +2
Query: 350 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 454
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.46
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 106 KTHINIVVIGHVDSGKST 159
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.6 bits (51), Expect = 0.80
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = +1
Query: 91 EMGKEKTHINIVVIGHVDSGKST 159
E+ + INI IGHV GKST
Sbjct: 35 EVISRQATINIGTIGHVAHGKST 57
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase
protein.
Length = 580
Score = 23.4 bits (48), Expect = 1.9
Identities = 8/23 (34%), Positives = 14/23 (60%)
Frame = +2
Query: 554 WIPLNHHTVSPDLRKSRRKYLIH 622
W+P+N + S +L +R+Y H
Sbjct: 443 WLPVNENYKSLNLAAQKREYYSH 465
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.4
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 335 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 436
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.2
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 335 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 436
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>DQ869051-1|ABJ09598.1| 581|Apis mellifera pyrokinin-like receptor
2 protein.
Length = 581
Score = 21.4 bits (43), Expect = 7.5
Identities = 9/30 (30%), Positives = 14/30 (46%)
Frame = -1
Query: 631 IFLMYEILPSGFPQIWAHCMVVQWNPFCLL 542
+ L+ LP IW+H V FC++
Sbjct: 100 LLLLISGLPPEIYYIWSHFPYVFGEAFCII 129
>DQ855484-1|ABH88171.1| 130|Apis mellifera chemosensory protein 3
protein.
Length = 130
Score = 21.0 bits (42), Expect = 9.9
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +1
Query: 196 DKRTIEKFEKEAQEMG 243
DK+ KFE+EA+++G
Sbjct: 112 DKKYRVKFEEEAKKLG 127
>AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor
protein.
Length = 1370
Score = 21.0 bits (42), Expect = 9.9
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = -1
Query: 496 LTGLTVLRDTSFEFTGTGSYDEHSAISLR 410
LTGLTVLR + + S E S R
Sbjct: 257 LTGLTVLRTFNASYNSLDSLPEGLFASTR 285
>AJ973401-1|CAJ01448.1| 130|Apis mellifera hypothetical protein
protein.
Length = 130
Score = 21.0 bits (42), Expect = 9.9
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +1
Query: 196 DKRTIEKFEKEAQEMG 243
DK+ KFE+EA+++G
Sbjct: 112 DKKFRVKFEEEAKKLG 127
>AF481963-1|AAN59784.1| 130|Apis mellifera antennal-specific
protein 3c precursor protein.
Length = 130
Score = 21.0 bits (42), Expect = 9.9
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +1
Query: 196 DKRTIEKFEKEAQEMG 243
DK+ KFE+EA+++G
Sbjct: 112 DKKYRVKFEEEAKKLG 127
>AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase
protein.
Length = 588
Score = 21.0 bits (42), Expect = 9.9
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = -2
Query: 606 LLDFLKSGLTVWWFSGIH 553
L F++SG+T W S I+
Sbjct: 56 LSHFIESGITAIWLSPIN 73
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 181,701
Number of Sequences: 438
Number of extensions: 3525
Number of successful extensions: 25
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 18826962
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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