Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060808.seq
         (635 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr...    43   2e-06
DQ384991-1|ABD51779.1|   94|Apis mellifera allergen Api m 6 vari...    23   2.5  
DQ384990-1|ABD51778.1|   92|Apis mellifera allergen Api m 6 vari...    23   2.5  
DQ244074-1|ABB36784.1|  517|Apis mellifera cytochrome P450 monoo...    23   2.5  
AY352277-2|AAQ67419.1|   88|Apis mellifera EX4.8-5.8 protein.          23   2.5  
DQ058012-1|AAY57281.1|  373|Apis mellifera venom allergen acid p...    22   5.7  
AY939855-1|AAX33235.1|  388|Apis mellifera venom acid phosphatas...    22   5.7  

>AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor
           protein.
          Length = 1370

 Score = 43.2 bits (97), Expect = 2e-06
 Identities = 29/65 (44%), Positives = 38/65 (58%), Gaps = 2/65 (3%)
 Frame = +3

Query: 324 KVTAVHNRAFVGLDTLEILTLYENRISVVDGEAFKGLEKKLKRLNLGGNELTAVPQ--KA 497
           K+  V  + F GL  L  LTL  N I+ +D  AF+     LK L+L GNELT+VP   + 
Sbjct: 394 KLRTVGAQLFNGLFVLNRLTLSGNAIASIDPLAFRNCSD-LKELDLSGNELTSVPDALRD 452

Query: 498 LALLR 512
           LALL+
Sbjct: 453 LALLK 457



 Score = 28.7 bits (61), Expect = 0.050
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +2

Query: 515 LKKLEMQENRITSISEGDFAGLRNLDSLGLAHNQL 619
           LK L++ ENRI++   G F  L  L  L L  N +
Sbjct: 456 LKTLDLGENRISNFYNGSFRNLDQLTGLRLIGNDI 490



 Score = 25.0 bits (52), Expect = 0.61
 Identities = 14/37 (37%), Positives = 20/37 (54%)
 Frame = +2

Query: 485 PAKSAGSSENLKKLEMQENRITSISEGDFAGLRNLDS 595
           P  S  S +NL+ L + ENR+  I++    GL   DS
Sbjct: 163 PVNSLCSLDNLQTLNLTENRLRDIND---IGLNRRDS 196



 Score = 23.4 bits (48), Expect = 1.9
 Identities = 12/47 (25%), Positives = 22/47 (46%)
 Frame = +3

Query: 336 VHNRAFVGLDTLEILTLYENRISVVDGEAFKGLEKKLKRLNLGGNEL 476
           + N  F+G   + +L +  + I  +    F GL   L+ L+L  N +
Sbjct: 809 LQNHVFIGRKNMRVLYVNGSGIESIQNRTFNGL-NNLQILHLEDNRI 854



 Score = 22.6 bits (46), Expect = 3.3
 Identities = 17/45 (37%), Positives = 23/45 (51%)
 Frame = +3

Query: 336  VHNRAFVGLDTLEILTLYENRISVVDGEAFKGLEKKLKRLNLGGN 470
            + N  F+ L +LEIL L  NR+ V        L  +L  L+LG N
Sbjct: 881  IGNLTFLPLRSLEILRLSGNRL-VTFPVWQVTLNARLVELSLGSN 924


>DQ384991-1|ABD51779.1|   94|Apis mellifera allergen Api m 6 variant
           2 precursor protein.
          Length = 94

 Score = 23.0 bits (47), Expect = 2.5
 Identities = 9/17 (52%), Positives = 10/17 (58%)
 Frame = -3

Query: 372 LGYQVRRMLCCVPRSPC 322
           LGY   +   CVPRS C
Sbjct: 75  LGYLRNKKKVCVPRSKC 91


>DQ384990-1|ABD51778.1|   92|Apis mellifera allergen Api m 6 variant
           1 precursor protein.
          Length = 92

 Score = 23.0 bits (47), Expect = 2.5
 Identities = 9/17 (52%), Positives = 10/17 (58%)
 Frame = -3

Query: 372 LGYQVRRMLCCVPRSPC 322
           LGY   +   CVPRS C
Sbjct: 75  LGYLRNKKKVCVPRSKC 91


>DQ244074-1|ABB36784.1|  517|Apis mellifera cytochrome P450
           monooxygenase protein.
          Length = 517

 Score = 23.0 bits (47), Expect = 2.5
 Identities = 4/19 (21%), Positives = 13/19 (68%)
 Frame = -1

Query: 101 MLFLNKAKPSYWYWKSQEY 45
           ++F+   +P++W+W +  +
Sbjct: 19  LIFVTSHRPAWWFWTATSH 37


>AY352277-2|AAQ67419.1|   88|Apis mellifera EX4.8-5.8 protein.
          Length = 88

 Score = 23.0 bits (47), Expect = 2.5
 Identities = 14/38 (36%), Positives = 18/38 (47%)
 Frame = +2

Query: 509 ENLKKLEMQENRITSISEGDFAGLRNLDSLGLAHNQLR 622
           EN    E +EN    I + DFA  +N     + HN LR
Sbjct: 5   ENSNMTEFEENVSFDIDDPDFAPSKNTT---ITHNILR 39


>DQ058012-1|AAY57281.1|  373|Apis mellifera venom allergen acid
           phosphatase protein.
          Length = 373

 Score = 21.8 bits (44), Expect = 5.7
 Identities = 11/34 (32%), Positives = 17/34 (50%), Gaps = 4/34 (11%)
 Frame = -3

Query: 249 LGDV----QLSQLVAFYDSFNLPINLQYGGIYAP 160
           LGD+     +S L +FYD   + + L    +Y P
Sbjct: 64  LGDIYTEESVSALSSFYDRTKMSLQLVLAALYPP 97


>AY939855-1|AAX33235.1|  388|Apis mellifera venom acid phosphatase
           precursor protein.
          Length = 388

 Score = 21.8 bits (44), Expect = 5.7
 Identities = 11/34 (32%), Positives = 17/34 (50%), Gaps = 4/34 (11%)
 Frame = -3

Query: 249 LGDV----QLSQLVAFYDSFNLPINLQYGGIYAP 160
           LGD+     +S L +FYD   + + L    +Y P
Sbjct: 79  LGDIYTEESVSALSSFYDRTKMSLQLVLAALYPP 112


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 173,497
Number of Sequences: 438
Number of extensions: 3648
Number of successful extensions: 14
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 13
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 19071468
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)