BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060805.seq (651 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC21D10.11c |nfs1||iron-sulfur cluster assembly protein Nfs1|S... 130 1e-31 SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 25 7.2 SPBC365.07c |||TATA element modulatory factor homolog |Schizosac... 25 7.2 SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyce... 25 9.5 SPBC336.07 |sfc3||transcription factor TFIIIC complex subunit Sf... 25 9.5 >SPBC21D10.11c |nfs1||iron-sulfur cluster assembly protein Nfs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 498 Score = 130 bits (315), Expect = 1e-31 Identities = 57/89 (64%), Positives = 71/89 (79%) Frame = +2 Query: 266 PLYFDAQATTPMDPRVLDVMLPYLVSYHGNPHSRTHAYGWESEAAVEKAREQVANLINAE 445 P+Y D QAT+P+D RVLD MLP+ +GNPHSRTHAYGWE+E AVE AR+++A++INA+ Sbjct: 100 PIYMDFQATSPLDYRVLDSMLPFFTGIYGNPHSRTHAYGWEAEKAVENARQEIASVINAD 159 Query: 446 PKEIIFTSGATESNNISVKGVVDFMHQEK 532 P+EIIFTSGATESNN +KGV F K Sbjct: 160 PREIIFTSGATESNNAILKGVARFYKSRK 188 Score = 66.9 bits (156), Expect = 2e-12 Identities = 29/47 (61%), Positives = 35/47 (74%) Frame = +1 Query: 511 RFYAPRKKHVITTQIEHKCVLDSCRALEGEGFRITYLPVPTEWNYNL 651 RFY RKKH+++ Q EHKCVLDS RAL+ EGF +T+LPV T NL Sbjct: 182 RFYKSRKKHLVSVQTEHKCVLDSLRALQEEGFEVTFLPVQTNGLINL 228 >SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces pombe|chr 1|||Manual Length = 4924 Score = 25.4 bits (53), Expect = 7.2 Identities = 13/44 (29%), Positives = 20/44 (45%) Frame = +3 Query: 153 IKQKTQLNGNGVVIFEQSNFFSDSANEKFSLKHEKLAGLCISML 284 I+ K Q + + V + N A FS + EKL C+S + Sbjct: 1190 IESKIQTHASFNVTITEHNHVESKARTGFSKREEKLVRSCLSKI 1233 >SPBC365.07c |||TATA element modulatory factor homolog |Schizosaccharomyces pombe|chr 2|||Manual Length = 547 Score = 25.4 bits (53), Expect = 7.2 Identities = 12/33 (36%), Positives = 21/33 (63%) Frame = +2 Query: 434 INAEPKEIIFTSGATESNNISVKGVVDFMHQEK 532 + ++PK+I+ T G E+N+ +V G VD + K Sbjct: 263 VKSKPKKIVTTGGIPENNDYTV-GKVDTLKVTK 294 >SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 344 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/40 (30%), Positives = 21/40 (52%) Frame = +3 Query: 408 KPENKWQI**MLNQKKLYLLQEQQNQITYLLKVWSILCTK 527 +PEN+W ++N + L E+Q +K W++L K Sbjct: 281 EPENRWCCESLVNYEALCKTLEKQKPTEADIKRWTLLVDK 320 >SPBC336.07 |sfc3||transcription factor TFIIIC complex subunit Sfc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 1339 Score = 25.0 bits (52), Expect = 9.5 Identities = 8/15 (53%), Positives = 12/15 (80%) Frame = -2 Query: 149 GFTNHRNHVFCQTEH 105 GF NH+NH+F +T + Sbjct: 1170 GFQNHKNHLFEETSN 1184 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,771,524 Number of Sequences: 5004 Number of extensions: 57560 Number of successful extensions: 147 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 142 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 147 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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