BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060805.seq (651 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g65720.1 68418.m08271 cysteine desulfurase, mitochondrial (NI... 129 1e-30 At1g08490.1 68414.m00940 cysteine desulfurase, putative similar ... 52 4e-07 At3g61940.1 68416.m06956 zinc transporter, putative similar to z... 29 2.7 At5g46400.1 68418.m05711 expressed protein 29 3.5 >At5g65720.1 68418.m08271 cysteine desulfurase, mitochondrial (NIFS) identical to Cysteine desulfurase, mitochondrial precursor (SP:O49543) {Arabidopsis thaliana}; identical to cDNA GI:12656131; contains Pfam profile PF00266: aminotransferase, class V Length = 453 Score = 129 bits (312), Expect = 1e-30 Identities = 59/91 (64%), Positives = 68/91 (74%) Frame = +2 Query: 260 GRPLYFDAQATTPMDPRVLDVMLPYLVSYHGNPHSRTHAYGWESEAAVEKAREQVANLIN 439 GRPLY D QATTP+DPRV D M + +GNPHSRTH YGWE+E AVE AR QVA LI Sbjct: 52 GRPLYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIE 111 Query: 440 AEPKEIIFTSGATESNNISVKGVVDFMHQEK 532 A PKEI+F SGATE+NN++VKGV+ F K Sbjct: 112 ASPKEIVFVSGATEANNMAVKGVMHFYKDTK 142 Score = 70.1 bits (164), Expect = 1e-12 Identities = 30/41 (73%), Positives = 33/41 (80%) Frame = +1 Query: 514 FYAPRKKHVITTQIEHKCVLDSCRALEGEGFRITYLPVPTE 636 FY KKHVITTQ EHKCVLDSCR L+ EGF +TYLPV T+ Sbjct: 137 FYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTD 177 >At1g08490.1 68414.m00940 cysteine desulfurase, putative similar to nitrogen fixation protein (nifS) GB:D64004 GI:1001701 from [Synechocystis sp]; contains TIGRFAM TIGR01364: phosphoserine aminotransferase; contains Pfam PF00266: aminotransferase, class V Length = 463 Score = 51.6 bits (118), Expect = 4e-07 Identities = 28/76 (36%), Positives = 42/76 (55%), Gaps = 1/76 (1%) Frame = +2 Query: 269 LYFDAQATTPMDPRVLDVMLPYLVSYHGNPHSRTHAYGWESEAAVEKAREQVANLINA-E 445 +Y D+ AT+ VLD + Y Y+ N H H ++ E AR++VA INA + Sbjct: 74 VYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFINASD 133 Query: 446 PKEIIFTSGATESNNI 493 +EI+FT ATE+ N+ Sbjct: 134 SREIVFTRNATEAINL 149 >At3g61940.1 68416.m06956 zinc transporter, putative similar to zinc transporter ZAT [Arabidopsis thaliana] gi|4206640|gb|AAD11757; similar to zinc transporter ZnT-2 [Rattus norvegicus] gi|1256378|gb|AAB02775; member of the cation diffusion facilitator (CDF) family, or cation efflux (CE) family, PMID:11500563 Length = 334 Score = 29.1 bits (62), Expect = 2.7 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = +2 Query: 347 HGNPHSRTHAYGWESEAAVEKARE 418 HG+ H H+YG +E +EK++E Sbjct: 166 HGHSHDHGHSYGERAEQLLEKSKE 189 >At5g46400.1 68418.m05711 expressed protein Length = 1036 Score = 28.7 bits (61), Expect = 3.5 Identities = 24/91 (26%), Positives = 40/91 (43%) Frame = +2 Query: 362 SRTHAYGWESEAAVEKAREQVANLINAEPKEIIFTSGATESNNISVKGVVDFMHQEKSMS 541 S +YG +S+ AV A+ +N EP + TSG N +V + +S Sbjct: 610 SSLDSYGTQSKDAVR------ADYVNTEPNQGCLTSGHLVEGNDNVIERETLCESQSDLS 663 Query: 542 LLLKLNTNVF*THVVL*KVKXSELHIYQSQQ 634 + LK N +H V ++ S H + ++Q Sbjct: 664 MGLKANEGGKRSHEVSLPIQASPEHGFVTKQ 694 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,957,541 Number of Sequences: 28952 Number of extensions: 280466 Number of successful extensions: 702 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 680 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 701 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1354097952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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