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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060805.seq
         (651 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g65720.1 68418.m08271 cysteine desulfurase, mitochondrial (NI...   129   1e-30
At1g08490.1 68414.m00940 cysteine desulfurase, putative similar ...    52   4e-07
At3g61940.1 68416.m06956 zinc transporter, putative similar to z...    29   2.7  
At5g46400.1 68418.m05711 expressed protein                             29   3.5  

>At5g65720.1 68418.m08271 cysteine desulfurase, mitochondrial (NIFS)
           identical to Cysteine desulfurase, mitochondrial
           precursor  (SP:O49543)  {Arabidopsis thaliana};
           identical to cDNA GI:12656131; contains Pfam profile
           PF00266: aminotransferase, class V
          Length = 453

 Score =  129 bits (312), Expect = 1e-30
 Identities = 59/91 (64%), Positives = 68/91 (74%)
 Frame = +2

Query: 260 GRPLYFDAQATTPMDPRVLDVMLPYLVSYHGNPHSRTHAYGWESEAAVEKAREQVANLIN 439
           GRPLY D QATTP+DPRV D M    +  +GNPHSRTH YGWE+E AVE AR QVA LI 
Sbjct: 52  GRPLYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIE 111

Query: 440 AEPKEIIFTSGATESNNISVKGVVDFMHQEK 532
           A PKEI+F SGATE+NN++VKGV+ F    K
Sbjct: 112 ASPKEIVFVSGATEANNMAVKGVMHFYKDTK 142



 Score = 70.1 bits (164), Expect = 1e-12
 Identities = 30/41 (73%), Positives = 33/41 (80%)
 Frame = +1

Query: 514 FYAPRKKHVITTQIEHKCVLDSCRALEGEGFRITYLPVPTE 636
           FY   KKHVITTQ EHKCVLDSCR L+ EGF +TYLPV T+
Sbjct: 137 FYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKTD 177


>At1g08490.1 68414.m00940 cysteine desulfurase, putative similar to
           nitrogen fixation protein (nifS) GB:D64004 GI:1001701
           from [Synechocystis sp]; contains TIGRFAM TIGR01364:
           phosphoserine aminotransferase; contains Pfam PF00266:
           aminotransferase, class V
          Length = 463

 Score = 51.6 bits (118), Expect = 4e-07
 Identities = 28/76 (36%), Positives = 42/76 (55%), Gaps = 1/76 (1%)
 Frame = +2

Query: 269 LYFDAQATTPMDPRVLDVMLPYLVSYHGNPHSRTHAYGWESEAAVEKAREQVANLINA-E 445
           +Y D+ AT+     VLD +  Y   Y+ N H   H    ++    E AR++VA  INA +
Sbjct: 74  VYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFINASD 133

Query: 446 PKEIIFTSGATESNNI 493
            +EI+FT  ATE+ N+
Sbjct: 134 SREIVFTRNATEAINL 149


>At3g61940.1 68416.m06956 zinc transporter, putative similar to zinc
           transporter ZAT [Arabidopsis thaliana]
           gi|4206640|gb|AAD11757; similar to zinc transporter
           ZnT-2 [Rattus norvegicus] gi|1256378|gb|AAB02775; member
           of the cation diffusion facilitator (CDF) family, or
           cation efflux (CE) family, PMID:11500563
          Length = 334

 Score = 29.1 bits (62), Expect = 2.7
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +2

Query: 347 HGNPHSRTHAYGWESEAAVEKARE 418
           HG+ H   H+YG  +E  +EK++E
Sbjct: 166 HGHSHDHGHSYGERAEQLLEKSKE 189


>At5g46400.1 68418.m05711 expressed protein
          Length = 1036

 Score = 28.7 bits (61), Expect = 3.5
 Identities = 24/91 (26%), Positives = 40/91 (43%)
 Frame = +2

Query: 362 SRTHAYGWESEAAVEKAREQVANLINAEPKEIIFTSGATESNNISVKGVVDFMHQEKSMS 541
           S   +YG +S+ AV       A+ +N EP +   TSG     N +V         +  +S
Sbjct: 610 SSLDSYGTQSKDAVR------ADYVNTEPNQGCLTSGHLVEGNDNVIERETLCESQSDLS 663

Query: 542 LLLKLNTNVF*THVVL*KVKXSELHIYQSQQ 634
           + LK N     +H V   ++ S  H + ++Q
Sbjct: 664 MGLKANEGGKRSHEVSLPIQASPEHGFVTKQ 694


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,957,541
Number of Sequences: 28952
Number of extensions: 280466
Number of successful extensions: 702
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 680
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 701
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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