Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060786.seq
(639 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000DB724B Cluster: PREDICTED: similar to eukaryotic... 109 4e-23
UniRef50_P62495 Cluster: Eukaryotic peptide chain release factor... 101 1e-20
UniRef50_Q8SRZ6 Cluster: PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1;... 74 3e-12
UniRef50_Q98RP4 Cluster: Eukaryotic release factor 1 homolog; n=... 68 2e-10
UniRef50_Q5CD84 Cluster: Eukaryotic peptide chain release factor... 63 6e-09
UniRef50_Q9NCP1 Cluster: Eukaryotic peptide chain release factor... 58 2e-07
UniRef50_Q9NAX8 Cluster: Eukaryotic peptide chain release factor... 52 2e-05
UniRef50_A2EPZ2 Cluster: Eukaryotic release factor 1, putative; ... 51 2e-05
UniRef50_P58227 Cluster: Peptide chain release factor subunit 1;... 44 0.004
UniRef50_Q8U0J4 Cluster: Peptide chain release factor subunit 1;... 42 0.017
UniRef50_Q965E7 Cluster: Eukaryotic polypeptide chain release fa... 41 0.022
UniRef50_Q58239 Cluster: Peptide chain release factor subunit 1;... 39 0.088
UniRef50_A7DMG3 Cluster: ERF1 domain 2 protein; n=1; Candidatus ... 38 0.27
UniRef50_A0RX46 Cluster: Peptide chain release factor 1; n=2; Cr... 38 0.27
UniRef50_Q8TS00 Cluster: Peptide chain release factor subunit 1-... 38 0.27
UniRef50_Q9HNF0 Cluster: Peptide chain release factor subunit 1;... 36 0.62
UniRef50_Q74N59 Cluster: NEQ052; n=1; Nanoarchaeum equitans|Rep:... 35 1.4
UniRef50_A2DQW1 Cluster: DHHC zinc finger domain containing prot... 33 7.7
>UniRef50_UPI0000DB724B Cluster: PREDICTED: similar to eukaryotic
release factor 1 CG5605-PA, isoform A; n=1; Apis
mellifera|Rep: PREDICTED: similar to eukaryotic release
factor 1 CG5605-PA, isoform A - Apis mellifera
Length = 265
Score = 109 bits (263), Expect = 4e-23
Identities = 63/97 (64%), Positives = 68/97 (70%), Gaps = 1/97 (1%)
Frame = +3
Query: 351 SEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADDL-VQLPI 527
+EE+SADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLAD+ I
Sbjct: 3 NEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNI 62
Query: 528 SSHV*IVSQCLAQLLLYSTDSSCTLKCHQTGLVIYCG 638
S V +S L + K GLVIYCG
Sbjct: 63 KSRVNRLS-VLGAITSVQHRLKLYTKVPPNGLVIYCG 98
>UniRef50_P62495 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=137; Eukaryota|Rep: Eukaryotic peptide
chain release factor subunit 1 - Homo sapiens (Human)
Length = 437
Score = 101 bits (242), Expect = 1e-20
Identities = 58/96 (60%), Positives = 65/96 (67%), Gaps = 1/96 (1%)
Frame = +3
Query: 354 EESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADDL-VQLPIS 530
+ S+ADRNVEIWKIKKLIKSLE ARGNGTSMISLIIPPKDQISRV+KMLAD+ I
Sbjct: 4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIK 63
Query: 531 SHV*IVSQCLAQLLLYSTDSSCTLKCHQTGLVIYCG 638
S V +S L + K GLV+YCG
Sbjct: 64 SRVNRLS-VLGAITSVQQRLKLYNKVPPNGLVVYCG 98
>UniRef50_Q8SRZ6 Cluster: PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1;
n=1; Encephalitozoon cuniculi|Rep: PEPTIDE CHAIN RELEASE
FACTOR SUBUNIT 1 - Encephalitozoon cuniculi
Length = 386
Score = 73.7 bits (173), Expect = 3e-12
Identities = 32/44 (72%), Positives = 41/44 (93%)
Frame = +3
Query: 378 VEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADD 509
VE+W++KKL+++L +RGNGTSMIS+IIPPKDQISRVSKML D+
Sbjct: 5 VEMWRMKKLLRNLRDSRGNGTSMISIIIPPKDQISRVSKMLTDE 48
Score = 65.3 bits (152), Expect = 1e-09
Identities = 30/37 (81%), Positives = 33/37 (89%)
Frame = +2
Query: 509 FGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNG 619
+GTASNIKSRVNRLSVLGAITS Q +LK Y+K PPNG
Sbjct: 49 YGTASNIKSRVNRLSVLGAITSAQSKLKQYSKTPPNG 85
>UniRef50_Q98RP4 Cluster: Eukaryotic release factor 1 homolog; n=1;
Guillardia theta|Rep: Eukaryotic release factor 1
homolog - Guillardia theta (Cryptomonas phi)
Length = 409
Score = 67.7 bits (158), Expect = 2e-10
Identities = 38/87 (43%), Positives = 50/87 (57%), Gaps = 1/87 (1%)
Frame = +3
Query: 381 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADDLVQLP-ISSHV*IVSQC 557
E+WKIKK + L +GNGTSMISL+IP + IS+VSKML+D+L I S + S
Sbjct: 9 ELWKIKKTYQYLSTIKGNGTSMISLVIPSNEHISKVSKMLSDELATASNIKSRI-NRSSV 67
Query: 558 LAQLLLYSTDSSCTLKCHQTGLVIYCG 638
L+ + K GL+IYCG
Sbjct: 68 LSAISSVQQKLKYYTKTPNNGLIIYCG 94
>UniRef50_Q5CD84 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=5; Eukaryota|Rep: Eukaryotic peptide chain
release factor subunit 1 - Loxodes striatus
Length = 436
Score = 62.9 bits (146), Expect = 6e-09
Identities = 35/86 (40%), Positives = 53/86 (61%), Gaps = 2/86 (2%)
Frame = +3
Query: 387 WKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADDLVQLP-ISSHV*IVSQCLA 563
WKI++LI+ LE A+GNGTS+ISLIIPPK+QIS +++ L D + I S V + +
Sbjct: 12 WKIRRLIEYLEKAKGNGTSLISLIIPPKEQISLINQKLTDAHGRAQNIKSKA--VQKAVQ 69
Query: 564 QLLLYSTDSSCTLK-CHQTGLVIYCG 638
++ + +K GL++YCG
Sbjct: 70 DAIISTKQKLSVIKQIPPNGLILYCG 95
>UniRef50_Q9NCP1 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=2; Giardia intestinalis|Rep: Eukaryotic
peptide chain release factor subunit 1 - Giardia lamblia
(Giardia intestinalis)
Length = 457
Score = 58.0 bits (134), Expect = 2e-07
Identities = 26/37 (70%), Positives = 31/37 (83%)
Frame = +2
Query: 509 FGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNG 619
+GTASNIKSRVN+ +VL AITS RLKLY ++PPNG
Sbjct: 62 YGTASNIKSRVNKNAVLSAITSAMSRLKLYNRLPPNG 98
Score = 54.4 bits (125), Expect = 2e-06
Identities = 31/91 (34%), Positives = 51/91 (56%), Gaps = 1/91 (1%)
Frame = +3
Query: 369 DRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADDL-VQLPISSHV*I 545
+R + IW++K+ I+ LE G+GTS+ISL+IPP +Q+S V+ ML ++ I S V
Sbjct: 15 ERQIAIWQLKRTIEKLESYTGSGTSVISLLIPPGEQLSSVTGMLTNEYGTASNIKSRV-N 73
Query: 546 VSQCLAQLLLYSTDSSCTLKCHQTGLVIYCG 638
+ L+ + + + GL +YCG
Sbjct: 74 KNAVLSAITSAMSRLKLYNRLPPNGLAVYCG 104
>UniRef50_Q9NAX8 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=6; Trypanosomatidae|Rep: Eukaryotic peptide
chain release factor subunit 1 - Trypanosoma brucei
brucei
Length = 452
Score = 51.6 bits (118), Expect = 2e-05
Identities = 35/96 (36%), Positives = 53/96 (55%), Gaps = 1/96 (1%)
Frame = +3
Query: 354 EESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQIS-RVSKMLADDLVQLPIS 530
E S +++ +E +K+KKLI+ LE ARG GTS+IS+ + PK+QIS V+K+ + I
Sbjct: 5 ELSESEKAIERYKVKKLIQMLESARGMGTSVISIYMTPKEQISGMVTKLNNEYGTASNIK 64
Query: 531 SHV*IVSQCLAQLLLYSTDSSCTLKCHQTGLVIYCG 638
SH +S A + GL++YCG
Sbjct: 65 SHTNKLSVQSAITASLGRLKQYN-RLPPNGLLLYCG 99
>UniRef50_A2EPZ2 Cluster: Eukaryotic release factor 1, putative;
n=2; Trichomonas vaginalis|Rep: Eukaryotic release
factor 1, putative - Trichomonas vaginalis G3
Length = 438
Score = 51.2 bits (117), Expect = 2e-05
Identities = 21/45 (46%), Positives = 34/45 (75%)
Frame = +3
Query: 378 VEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADDL 512
+E++KIK+LI L A G+GTSM++++IPP D + R+ + L D+L
Sbjct: 14 IELFKIKRLINYLRDAHGDGTSMVTILIPPGDAVHRMRQKLTDEL 58
Score = 44.4 bits (100), Expect = 0.002
Identities = 21/36 (58%), Positives = 25/36 (69%)
Frame = +2
Query: 512 GTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNG 619
GTASNIK+RVNR SV AIT+ +L + PPNG
Sbjct: 59 GTASNIKNRVNRQSVEAAITAAIQKLGQFNSTPPNG 94
>UniRef50_P58227 Cluster: Peptide chain release factor subunit 1;
n=5; Thermoplasmatales|Rep: Peptide chain release factor
subunit 1 - Thermoplasma volcanium
Length = 418
Score = 43.6 bits (98), Expect = 0.004
Identities = 31/84 (36%), Positives = 42/84 (50%), Gaps = 4/84 (4%)
Frame = +2
Query: 380 RNMEDKETYKEL----GNGQRKWNIYDIPDHST*GSNLESIKDVG**FGTASNIKSRVNR 547
R E K +EL G G ++Y IP + ++D + T+SNIKS+ R
Sbjct: 11 RRYEFKRALEELSKLHGRGTELISLY-IPPEKQISDVVAYLRDE---YSTSSNIKSKSTR 66
Query: 548 LSVLGAITSVQHRLKLYTKVPPNG 619
+VL AI S+ RLK Y PPNG
Sbjct: 67 KNVLAAIESIMSRLKYYRFPPPNG 90
Score = 40.3 bits (90), Expect = 0.038
Identities = 18/47 (38%), Positives = 27/47 (57%)
Frame = +3
Query: 369 DRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADD 509
D + ++ K+ ++ L G GT +ISL IPP+ QIS V L D+
Sbjct: 7 DEQIRRYEFKRALEELSKLHGRGTELISLYIPPEKQISDVVAYLRDE 53
>UniRef50_Q8U0J4 Cluster: Peptide chain release factor subunit 1;
n=5; Archaea|Rep: Peptide chain release factor subunit 1
- Pyrococcus furiosus
Length = 420
Score = 41.5 bits (93), Expect = 0.017
Identities = 18/37 (48%), Positives = 23/37 (62%)
Frame = +2
Query: 509 FGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNG 619
+ TA NIKS+ R +VLGA+ LKLY + P NG
Sbjct: 53 YSTAQNIKSKTTRKNVLGALERAMQHLKLYKQTPENG 89
Score = 39.5 bits (88), Expect = 0.067
Identities = 17/46 (36%), Positives = 30/46 (65%)
Frame = +3
Query: 372 RNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADD 509
R+ +++++KK I L+ RG GT +ISL IP +S+V + L ++
Sbjct: 7 RDAQLYELKKKIDELKKIRGRGTELISLYIPAGYDLSKVMQQLREE 52
>UniRef50_Q965E7 Cluster: Eukaryotic polypeptide chain release
factor 1; n=3; Paramecium tetraurelia|Rep: Eukaryotic
polypeptide chain release factor 1 - Paramecium
tetraurelia
Length = 437
Score = 41.1 bits (92), Expect = 0.022
Identities = 20/47 (42%), Positives = 32/47 (68%)
Frame = +3
Query: 360 SSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKML 500
+ A+ +E +++KKLIK+L R GTS++S+ IPPK IS ++ L
Sbjct: 6 NDAEIALEQFRLKKLIKTLSQERTAGTSVVSVYIPPKRIISDITNRL 52
Score = 33.1 bits (72), Expect = 5.8
Identities = 16/37 (43%), Positives = 23/37 (62%)
Frame = +2
Query: 509 FGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNG 619
+ A++IK + NR+SV AI + RL+ Y K P NG
Sbjct: 56 YAEAASIKDKGNRISVQEAIQAAILRLRPYNKAPNNG 92
>UniRef50_Q58239 Cluster: Peptide chain release factor subunit 1;
n=12; Euryarchaeota|Rep: Peptide chain release factor
subunit 1 - Methanococcus jannaschii
Length = 421
Score = 39.1 bits (87), Expect = 0.088
Identities = 17/46 (36%), Positives = 31/46 (67%)
Frame = +3
Query: 381 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADDLVQ 518
+++ KK++K L+ +G GT +ISL IP +IS V++ L +++ Q
Sbjct: 8 QLYLFKKMLKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQ 53
>UniRef50_A7DMG3 Cluster: ERF1 domain 2 protein; n=1; Candidatus
Nitrosopumilus maritimus SCM1|Rep: ERF1 domain 2 protein
- Candidatus Nitrosopumilus maritimus SCM1
Length = 426
Score = 37.5 bits (83), Expect = 0.27
Identities = 18/36 (50%), Positives = 22/36 (61%)
Frame = +2
Query: 512 GTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNG 619
GTA NIKS + R V+ ++ V RLKLY K P G
Sbjct: 57 GTADNIKSDLTRSHVVDSLGKVVQRLKLYKKTPERG 92
>UniRef50_A0RX46 Cluster: Peptide chain release factor 1; n=2;
Crenarchaeota|Rep: Peptide chain release factor 1 -
Cenarchaeum symbiosum
Length = 411
Score = 37.5 bits (83), Expect = 0.27
Identities = 17/36 (47%), Positives = 22/36 (61%)
Frame = +2
Query: 512 GTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNG 619
GTA NIKS + R V+ ++ V RL+LY K P G
Sbjct: 44 GTADNIKSDLTRTHVVDSLAKVVQRLRLYKKTPERG 79
>UniRef50_Q8TS00 Cluster: Peptide chain release factor subunit 1-2;
n=9; Euryarchaeota|Rep: Peptide chain release factor
subunit 1-2 - Methanosarcina acetivorans
Length = 415
Score = 37.5 bits (83), Expect = 0.27
Identities = 18/43 (41%), Positives = 27/43 (62%)
Frame = +3
Query: 381 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADD 509
E + KK +++L+ G T +ISL IPP QIS V+K L ++
Sbjct: 8 EKYVFKKQLETLKSKNGRSTELISLYIPPDKQISDVTKHLREE 50
>UniRef50_Q9HNF0 Cluster: Peptide chain release factor subunit 1;
n=7; Euryarchaeota|Rep: Peptide chain release factor
subunit 1 - Halobacterium salinarium (Halobacterium
halobium)
Length = 416
Score = 36.3 bits (80), Expect = 0.62
Identities = 16/33 (48%), Positives = 23/33 (69%)
Frame = +2
Query: 518 ASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPN 616
ASNIKS+ R +V A+TS++ RL+ Y PP+
Sbjct: 59 ASNIKSKQTRTNVQDALTSIKDRLRYYDTFPPD 91
Score = 34.3 bits (75), Expect = 2.5
Identities = 17/49 (34%), Positives = 30/49 (61%)
Frame = +3
Query: 342 NKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRV 488
++ E S DR ++ +K+I+ L+ G+GT ++++ IPP QIS V
Sbjct: 2 SEQDEVPSEDRRK--YEFRKVIEELKDYEGSGTQLVTIYIPPDKQISDV 48
>UniRef50_Q74N59 Cluster: NEQ052; n=1; Nanoarchaeum equitans|Rep:
NEQ052 - Nanoarchaeum equitans
Length = 358
Score = 35.1 bits (77), Expect = 1.4
Identities = 25/89 (28%), Positives = 42/89 (47%), Gaps = 3/89 (3%)
Frame = +3
Query: 381 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADD---LVQLPISSHV*IVS 551
E +K+K L++ L+ G GT ++SL IP I ++ L + + + V
Sbjct: 5 EKYKLKSLLRQLKSIEGRGTELVSLYIPSNYPIGKILDQLNYEYATAANIKDDTTRKNVQ 64
Query: 552 QCLAQLLLYSTDSSCTLKCHQTGLVIYCG 638
LA+++ Y D K + GLVI+ G
Sbjct: 65 AALAKIINYLKDFK---KIPENGLVIFAG 90
>UniRef50_A2DQW1 Cluster: DHHC zinc finger domain containing
protein; n=1; Trichomonas vaginalis G3|Rep: DHHC zinc
finger domain containing protein - Trichomonas vaginalis
G3
Length = 347
Score = 32.7 bits (71), Expect = 7.7
Identities = 19/55 (34%), Positives = 25/55 (45%)
Frame = -2
Query: 395 YLPYFDISIGRRFFRHFVSLFI*LINHKHYICINN*NNVFAQHQNFKKRILWKGS 231
Y P+ + IG R R+F+ I I Y CIN+ VF +RI W S
Sbjct: 172 YCPWVLVPIGERNHRYFLLFLIACIVASTYYCINDVFVVFFYFMQVYQRIPWTNS 226
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 623,587,045
Number of Sequences: 1657284
Number of extensions: 11909263
Number of successful extensions: 29524
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 28596
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29512
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 47711253245
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -