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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060786.seq
         (639 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    73   5e-14
SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi...    28   1.3  
SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr...    27   2.3  
SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb...    27   3.0  
SPBC1347.06c |cki1||serine/threonine protein kinase Cki1|Schizos...    25   9.2  
SPBC887.04c |lub1||WD repeat protein Lub1|Schizosaccharomyces po...    25   9.2  

>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 72.5 bits (170), Expect = 5e-14
 Identities = 48/100 (48%), Positives = 61/100 (61%), Gaps = 3/100 (3%)
 Frame = +3

Query: 348 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADDL-VQLP 524
           MSE  +A++ +EIWKI++L+K L    GNGTSMI+LIIPP +QISR S MLA++      
Sbjct: 1   MSE--TAEKAIEIWKIRRLVKQLINCHGNGTSMITLIIPPGEQISRYSNMLAEEYGTASN 58

Query: 525 ISSHV*IVSQCLAQLLLYSTDSSCTL--KCHQTGLVIYCG 638
           I S V  +S   A   + ST     L  K    GLVIYCG
Sbjct: 59  IKSRVNRLSVLSA---ITSTRERLKLYNKVPDNGLVIYCG 95


>SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA
           reductase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1053

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 13/37 (35%), Positives = 21/37 (56%)
 Frame = +3

Query: 429 GNGTSMISLIIPPKDQISRVSKMLADDLVQLPISSHV 539
           G G S+I+  I P D +  V K   +DLV+L +  ++
Sbjct: 856 GRGKSVIAEAIIPGDAVKSVLKTTVEDLVKLNVDKNL 892


>SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 749

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
 Frame = +3

Query: 327 LNKKRNKMSEE--SSADRNVEIWKIKKLIKSLEMARGNGTS 443
           LN+  N +S    SSAD  +E W   K I S + +R   TS
Sbjct: 10  LNRLNNGLSSNNGSSADEGLEHWNPLKKIHSADSSRRRSTS 50


>SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 440

 Score = 26.6 bits (56), Expect = 3.0
 Identities = 13/31 (41%), Positives = 21/31 (67%), Gaps = 2/31 (6%)
 Frame = -2

Query: 347 FVSLFI*LINHKHYIC-INN*NNVF-AQHQN 261
           F + F+ L +HK+YIC + N NN++  QH +
Sbjct: 197 FSTKFVALSSHKNYICGLTNDNNLYILQHSH 227


>SPBC1347.06c |cki1||serine/threonine protein kinase
           Cki1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 446

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 11/31 (35%), Positives = 15/31 (48%)
 Frame = -1

Query: 216 QTALI*FEPDGRDTSRLRSHFLVYIYVCGCS 124
           Q   I FEP   D  +LR  +  Y  + GC+
Sbjct: 36  QQVAIKFEPRRSDAPQLRDEYRTYKLLAGCT 66


>SPBC887.04c |lub1||WD repeat protein Lub1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 713

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 9/18 (50%), Positives = 15/18 (83%)
 Frame = +3

Query: 363 SADRNVEIWKIKKLIKSL 416
           SAD+ ++IW  +KL+KS+
Sbjct: 155 SADKLIKIWNGEKLVKSI 172


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,665,131
Number of Sequences: 5004
Number of extensions: 53271
Number of successful extensions: 162
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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