BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060786.seq (639 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g47880.1 68418.m05915 eukaryotic peptide chain release factor... 89 2e-18 At3g26618.1 68416.m03325 eukaryotic release factor 1 family prot... 87 1e-17 At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot... 87 1e-17 At4g02420.1 68417.m00327 lectin protein kinase, putative similar... 30 1.5 At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains ... 29 2.0 At4g14480.1 68417.m02233 protein kinase family protein contains ... 29 3.4 At3g59610.1 68416.m06651 F-box family protein / jacalin lectin f... 28 6.0 At2g44830.1 68415.m05582 protein kinase, putative similar to pro... 27 7.9 At1g60280.1 68414.m06788 no apical meristem (NAM) protein-relate... 27 7.9 >At5g47880.1 68418.m05915 eukaryotic peptide chain release factor subunit 1-1 (ERF1-1) identical to SP|Q39097 Eukaryotic peptide chain release factor subunit 1-1 (eRF1-1) (Eukaryotic release factor 1-1) (Omnipotent suppressor protein 1 homolog 1) (SUP1 homolog 1) {Arabidopsis thaliana}, eukaryotic release factor 1 homolog GI:1155261 from [Arabidopsis thaliana]; contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 436 Score = 89.4 bits (212), Expect = 2e-18 Identities = 39/54 (72%), Positives = 49/54 (90%) Frame = +3 Query: 348 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADD 509 M +++ D+N+EIWKIKKLIKSLE ARGNGTSMISLI+PP+DQ+SRV+KML D+ Sbjct: 1 MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDE 54 Score = 67.3 bits (157), Expect = 8e-12 Identities = 33/43 (76%), Positives = 36/43 (83%) Frame = +2 Query: 491 KDVG**FGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNG 619 K +G +GTASNIKSRVNR SVLGAITS Q RLKLY +VPPNG Sbjct: 49 KMLGDEYGTASNIKSRVNRQSVLGAITSAQQRLKLYNRVPPNG 91 >At3g26618.1 68416.m03325 eukaryotic release factor 1 family protein / eRF1 family protein contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 435 Score = 86.6 bits (205), Expect = 1e-17 Identities = 37/51 (72%), Positives = 47/51 (92%) Frame = +3 Query: 357 ESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADD 509 + +D+N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ++RV+KMLAD+ Sbjct: 3 DQESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADE 53 Score = 65.7 bits (153), Expect = 2e-11 Identities = 31/37 (83%), Positives = 32/37 (86%) Frame = +2 Query: 509 FGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNG 619 +GTASNIKSRVNR SVL AITS Q RLKLY KVPPNG Sbjct: 54 YGTASNIKSRVNRQSVLSAITSAQQRLKLYNKVPPNG 90 >At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein / eRF1 family protein contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 434 Score = 86.6 bits (205), Expect = 1e-17 Identities = 41/54 (75%), Positives = 48/54 (88%) Frame = +3 Query: 348 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADD 509 M+EE AD N+EIWKIKKLIK LE ARGNGTSMISLI+PP+DQ+SRV+KML D+ Sbjct: 1 MAEE--ADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDE 52 Score = 62.9 bits (146), Expect = 2e-10 Identities = 32/43 (74%), Positives = 34/43 (79%) Frame = +2 Query: 491 KDVG**FGTASNIKSRVNRLSVLGAITSVQHRLKLYTKVPPNG 619 K +G +GTASNIKSRVNR SVL AITS Q RLKLY KVP NG Sbjct: 47 KMLGDEYGTASNIKSRVNRQSVLSAITSAQQRLKLYNKVPTNG 89 >At4g02420.1 68417.m00327 lectin protein kinase, putative similar to receptor lectin kinase 3 [Arabidopsis thaliana] gi|4100060|gb|AAD00733; contains protein kinase domain, Pfam:PF00069; contains legume lectins alpha and beta domains, Pfam:PF00138 and Pfam:PF00139 Length = 669 Score = 29.9 bits (64), Expect = 1.5 Identities = 19/60 (31%), Positives = 30/60 (50%) Frame = +3 Query: 246 YSFFKVLVLCKDIILVIYTNIVFVVN*LNKKRNKMSEESSADRNVEIWKIKKLIKSLEMA 425 Y F+K V ++L+ + I+F+V + K+R K +EE D E K + K L A Sbjct: 288 YRFYKNWVPLISLLLIPFLLIIFLVRFIMKRRRKFAEEVE-DWETEFGKNRLRFKDLYYA 346 >At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains similarity to NAD kinase [Homo sapiens] gi|20070086|gb|AAM01195; contains Pfam domain, PF01513: ATP-NAD kinase Length = 985 Score = 29.5 bits (63), Expect = 2.0 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +3 Query: 408 KSLEMARGNGTSMISLIIPPKDQISR 485 KS+ RGNG S + +PP D +SR Sbjct: 561 KSISDNRGNGFSAAPIAVPPSDNLSR 586 >At4g14480.1 68417.m02233 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 487 Score = 28.7 bits (61), Expect = 3.4 Identities = 16/54 (29%), Positives = 30/54 (55%) Frame = +3 Query: 339 RNKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKML 500 +N+ E D+++ + K+K L KSLE R + +I + K++ SR ++L Sbjct: 407 KNQEGEVVGFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELL 460 >At3g59610.1 68416.m06651 F-box family protein / jacalin lectin family protein contains Pfam profile PF01419: Jacalin-like lectin domain Length = 521 Score = 27.9 bits (59), Expect = 6.0 Identities = 12/45 (26%), Positives = 25/45 (55%) Frame = +3 Query: 348 MSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQIS 482 + +E RN+EIW K+ IK+ + +++S+++P +S Sbjct: 251 LEQEIYNTRNIEIWVTKETIKNGDGEAVEWVNLMSVLVPEWSSLS 295 >At2g44830.1 68415.m05582 protein kinase, putative similar to protein kinase PVPK-1 [Phaseolus vulgaris] SWISS-PROT:P15792 Length = 765 Score = 27.5 bits (58), Expect = 7.9 Identities = 16/44 (36%), Positives = 24/44 (54%) Frame = +3 Query: 402 LIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADDLVQLPISS 533 L+KS+ +G ISL +PPK RV A+ +V+ P +S Sbjct: 172 LMKSMSTTKGPFIEDISLCVPPKKPSPRVLSP-AESIVEEPATS 214 >At1g60280.1 68414.m06788 no apical meristem (NAM) protein-related contains Pfam PF02365: No apical meristem (NAM) protein; contains similarity to NAM-like protein GI:4337200 from [Arabidopsis thaliana] Length = 347 Score = 27.5 bits (58), Expect = 7.9 Identities = 11/28 (39%), Positives = 16/28 (57%) Frame = +1 Query: 460 FHLRIKSREYQRCWLMIWYSFQYQVTCE 543 F L+ K REY+R W+M Y + C+ Sbjct: 128 FCLKKKPREYKRSWVMEEYRLNNNLNCK 155 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,783,910 Number of Sequences: 28952 Number of extensions: 276991 Number of successful extensions: 697 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 672 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 697 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1314848736 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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