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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060782.seq
         (574 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ...   105   2e-23
At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ...   101   3e-22
At5g17770.1 68418.m02084 NADH-cytochrome b5 reductase identical ...    55   3e-08
At5g20080.1 68418.m02391 NADH-cytochrome b5 reductase, putative ...    46   2e-05
At2g42480.1 68415.m05255 meprin and TRAF homology domain-contain...    31   0.41 
At5g65600.1 68418.m08253 legume lectin family protein / protein ...    29   2.9  

>At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to
           SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1)
           (NR1){Arabidopsis thaliana}
          Length = 917

 Score =  105 bits (252), Expect = 2e-23
 Identities = 44/90 (48%), Positives = 65/90 (72%)
 Frame = +3

Query: 246 QEIRLGLPSPQHVLGLPIGQHIHLSAKINDDLVIRAYTPVSSDEDKGYVDLVIKVYFKNV 425
           ++ R  LPS    LGLP+G+H+ + A IND L +RAYTP S+ +  G++DLV+KVYFK+V
Sbjct: 678 RKFRFALPSEDQQLGLPVGKHVFVCANINDKLCLRAYTPTSAIDAVGHIDLVVKVYFKDV 737

Query: 426 HPKFPEGGKLSQYLNNMKINDTIDVRXTSG 515
           HP+FP GG +SQ+L+++ I   ID++   G
Sbjct: 738 HPRFPNGGLMSQHLDSLPIGSMIDIKGPLG 767


>At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to
           SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1)
           (NR2) {Arabidopsis thaliana}
          Length = 917

 Score =  101 bits (243), Expect = 3e-22
 Identities = 44/90 (48%), Positives = 63/90 (70%)
 Frame = +3

Query: 246 QEIRLGLPSPQHVLGLPIGQHIHLSAKINDDLVIRAYTPVSSDEDKGYVDLVIKVYFKNV 425
           ++ R  LP    VLGLP+G+HI L A IND L +RAYTP S+ +  GY +LV+K+YF  V
Sbjct: 678 RKFRFALPVEDMVLGLPVGKHIFLCATINDKLCLRAYTPSSTVDVVGYFELVVKIYFGGV 737

Query: 426 HPKFPEGGKLSQYLNNMKINDTIDVRXTSG 515
           HP+FP GG +SQYL+++ I  T++++   G
Sbjct: 738 HPRFPNGGLMSQYLDSLPIGSTLEIKGPLG 767


>At5g17770.1 68418.m02084 NADH-cytochrome b5 reductase identical to
           NADH-cytochrome b5 reductase [Arabidopsis thaliana]
           GI:4240116
          Length = 281

 Score = 55.2 bits (127), Expect = 3e-08
 Identities = 33/85 (38%), Positives = 47/85 (55%), Gaps = 1/85 (1%)
 Frame = +3

Query: 264 LPSPQHVLGLPIGQHIHLSAKIND-DLVIRAYTPVSSDEDKGYVDLVIKVYFKNVHPKFP 440
           LP+   VLGLPIGQHI    K    + VI+ YTP + D D G  +LVIK+Y        P
Sbjct: 69  LPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMY--------P 120

Query: 441 EGGKLSQYLNNMKINDTIDVRXTSG 515
           + G++S +   M++ D + V+   G
Sbjct: 121 Q-GRMSHHFREMRVGDHLAVKGPKG 144


>At5g20080.1 68418.m02391 NADH-cytochrome b5 reductase, putative
           similar to SP|P36060 NADH-cytochrome b5 reductase
           precursor (EC 1.6.2.2) {Saccharomyces cerevisiae};
           contains Pfam profiles PF00175: Oxidoreductase
           NAD-binding domain, PF00970: oxidoreductase, FAD-binding
          Length = 328

 Score = 46.0 bits (104), Expect = 2e-05
 Identities = 25/54 (46%), Positives = 35/54 (64%)
 Frame = +3

Query: 342 VIRAYTPVSSDEDKGYVDLVIKVYFKNVHPKFPEGGKLSQYLNNMKINDTIDVR 503
           VIR YTP+S  E KGY DL+IKVY        P+ GK+SQ+  ++K  D ++V+
Sbjct: 131 VIRPYTPISDPEAKGYFDLLIKVY--------PD-GKMSQHFASLKPGDVLEVK 175


>At2g42480.1 68415.m05255 meprin and TRAF homology domain-containing
           protein / MATH domain-containing protein similar to
           ubiquitin-specific protease 12 [Arabidopsis thaliana]
           GI:11993471; contains Pfam profile PF00917: MATH domain
          Length = 743

 Score = 31.5 bits (68), Expect = 0.41
 Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 3/42 (7%)
 Frame = +3

Query: 345 IRAYTPVSSDEDKGYVD---LVIKVYFKNVHPKFPEGGKLSQ 461
           IR   P+S  E+KG+++   L+++VY KN      EGG +S+
Sbjct: 105 IRKALPLSKFEEKGFLEKDKLIVEVYIKNFEAVDGEGGGVSK 146


>At5g65600.1 68418.m08253 legume lectin family protein / protein
           kinase family protein contains Pfam domains PF00138:
           Legume lectins alpha domain, PF00139: Legume lectins
           beta domain and PF00069: Protein kinase domain
          Length = 675

 Score = 28.7 bits (61), Expect = 2.9
 Identities = 19/52 (36%), Positives = 25/52 (48%), Gaps = 1/52 (1%)
 Frame = +3

Query: 387 YVDLVIKVYFKNVHPKFPEGGKLSQYLNNMK-INDTIDVRXTSGDCSTQETE 539
           Y DLV      + H K  EGG  + Y  N+K IN  + V+  SGD    + E
Sbjct: 340 YKDLVSATNRFSSHRKLGEGGFGAVYEGNLKEINTMVAVKKLSGDSRQGKNE 391


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,658,411
Number of Sequences: 28952
Number of extensions: 228932
Number of successful extensions: 493
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 480
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 492
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1112061928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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