BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060780.seq (448 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g56670.1 68418.m07074 40S ribosomal protein S30 (RPS30C) 52 1e-07 At4g29390.1 68417.m04198 40S ribosomal protein S30 (RPS30B) RIBO... 52 1e-07 At2g19750.1 68415.m02307 40S ribosomal protein S30 (RPS30A) 52 1e-07 At5g56850.2 68418.m07093 expressed protein 27 4.4 At5g56850.1 68418.m07094 expressed protein 27 4.4 At5g37640.1 68418.m04533 polyubiquitin (UBQ9) identical to polyu... 27 4.4 At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-rel... 27 4.4 At1g25320.1 68414.m03142 leucine-rich repeat transmembrane prote... 27 4.4 At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu... 27 5.8 >At5g56670.1 68418.m07074 40S ribosomal protein S30 (RPS30C) Length = 62 Score = 52.4 bits (120), Expect = 1e-07 Identities = 24/47 (51%), Positives = 30/47 (63%) Frame = +1 Query: 256 VKGQTPKVEXXXXXXXXTGRAKRRIQYNRRFVNVVQTFGRXXGPNSN 396 V+GQTPKV GRA +R+Q+NRRFV V FG+ GPNS+ Sbjct: 14 VRGQTPKVAKQDKKKKPRGRAHKRLQHNRRFVTAVVGFGKKRGPNSS 60 Score = 26.6 bits (56), Expect = 7.6 Identities = 11/11 (100%), Positives = 11/11 (100%) Frame = +2 Query: 221 GKVHGSLARAG 253 GKVHGSLARAG Sbjct: 2 GKVHGSLARAG 12 >At4g29390.1 68417.m04198 40S ribosomal protein S30 (RPS30B) RIBOSOMAL PROTEIN S30 - Arabidopsis thaliana,PID:e1358183 Length = 62 Score = 52.4 bits (120), Expect = 1e-07 Identities = 24/47 (51%), Positives = 30/47 (63%) Frame = +1 Query: 256 VKGQTPKVEXXXXXXXXTGRAKRRIQYNRRFVNVVQTFGRXXGPNSN 396 V+GQTPKV GRA +R+Q+NRRFV V FG+ GPNS+ Sbjct: 14 VRGQTPKVAKQDKKKKPRGRAHKRLQHNRRFVTAVVGFGKKRGPNSS 60 Score = 26.6 bits (56), Expect = 7.6 Identities = 11/11 (100%), Positives = 11/11 (100%) Frame = +2 Query: 221 GKVHGSLARAG 253 GKVHGSLARAG Sbjct: 2 GKVHGSLARAG 12 >At2g19750.1 68415.m02307 40S ribosomal protein S30 (RPS30A) Length = 62 Score = 52.4 bits (120), Expect = 1e-07 Identities = 24/47 (51%), Positives = 30/47 (63%) Frame = +1 Query: 256 VKGQTPKVEXXXXXXXXTGRAKRRIQYNRRFVNVVQTFGRXXGPNSN 396 V+GQTPKV GRA +R+Q+NRRFV V FG+ GPNS+ Sbjct: 14 VRGQTPKVAKQDKKKKPRGRAHKRLQHNRRFVTAVVGFGKKRGPNSS 60 Score = 26.6 bits (56), Expect = 7.6 Identities = 11/11 (100%), Positives = 11/11 (100%) Frame = +2 Query: 221 GKVHGSLARAG 253 GKVHGSLARAG Sbjct: 2 GKVHGSLARAG 12 >At5g56850.2 68418.m07093 expressed protein Length = 413 Score = 27.5 bits (58), Expect = 4.4 Identities = 11/30 (36%), Positives = 19/30 (63%) Frame = +2 Query: 2 GTRCSCISEGNRHTSWTSMARSPLVRSRNA 91 G+RC I++ N + WT++ RS +S N+ Sbjct: 353 GSRCINIAKINSNNKWTNIERSKKQKSENS 382 >At5g56850.1 68418.m07094 expressed protein Length = 551 Score = 27.5 bits (58), Expect = 4.4 Identities = 11/30 (36%), Positives = 19/30 (63%) Frame = +2 Query: 2 GTRCSCISEGNRHTSWTSMARSPLVRSRNA 91 G+RC I++ N + WT++ RS +S N+ Sbjct: 491 GSRCINIAKINSNNKWTNIERSKKQKSENS 520 >At5g37640.1 68418.m04533 polyubiquitin (UBQ9) identical to polyubiquitin (ubq9) gene sequence GI:304120 from [Arabidopsis thaliana] Length = 322 Score = 27.5 bits (58), Expect = 4.4 Identities = 15/60 (25%), Positives = 30/60 (50%), Gaps = 2/60 (3%) Frame = +1 Query: 10 MQLHIRG--QSTHVLDVNGQESIGQIKERIRTLAAVGDEDLTLSLCGAPLDDSCLVSELS 183 MQ+H + + T +DV ++I +K +I+ + + + L G LDD +++ S Sbjct: 3 MQIHAKTLTEKTITIDVVSSDTINNVKAKIQDIEGIPLDQQRLIFSGKLLDDGRTLADYS 62 >At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-related contains Pfam PF02138: Beige/BEACH domain; contains Pfam PF00400: WD domain, G-beta repeat (3 copies) Length = 2946 Score = 27.5 bits (58), Expect = 4.4 Identities = 12/33 (36%), Positives = 17/33 (51%) Frame = +1 Query: 19 HIRGQSTHVLDVNGQESIGQIKERIRTLAAVGD 117 H+R QS N S +K+R TL A+G+ Sbjct: 1385 HLRSQSKQTCATNAVASPSPLKKRTSTLTAIGE 1417 >At1g25320.1 68414.m03142 leucine-rich repeat transmembrane protein kinase, putative similar to putative receptor-like protein kinase GI:4262228 from [Arabidopsis thaliana] Length = 702 Score = 27.5 bits (58), Expect = 4.4 Identities = 12/22 (54%), Positives = 13/22 (59%) Frame = +1 Query: 136 LCGAPLDDSCLVSELSSSXTRP 201 LCG PL D CL SSS + P Sbjct: 273 LCGPPLKDPCLPDTDSSSTSHP 294 >At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis thaliana] (Genetics 139 (2), 921-939 (1995)) Length = 631 Score = 27.1 bits (57), Expect = 5.8 Identities = 15/61 (24%), Positives = 30/61 (49%), Gaps = 2/61 (3%) Frame = +1 Query: 1 RHEMQLHIRGQS--THVLDVNGQESIGQIKERIRTLAAVGDEDLTLSLCGAPLDDSCLVS 174 R MQ+ I+ + T +L+V ++I +KE+I+ + + L G L+D + Sbjct: 549 RGGMQIFIKTLTGKTIILEVESSDTIANVKEKIQVKEGIKPDQQMLIFFGQQLEDGVTLG 608 Query: 175 E 177 + Sbjct: 609 D 609 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,887,300 Number of Sequences: 28952 Number of extensions: 148422 Number of successful extensions: 507 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 486 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 506 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 722638680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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