BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060774.seq (630 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 117 2e-27 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 117 2e-27 SPAC12G12.07c |||conserved fungal protein|Schizosaccharomyces po... 27 2.2 SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 3.9 SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 26 5.2 SPAC3C7.07c |||arginine-tRNA protein transferase |Schizosaccharo... 25 9.0 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 117 bits (281), Expect = 2e-27 Identities = 59/108 (54%), Positives = 63/108 (58%), Gaps = 1/108 (0%) Frame = +3 Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434 QTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 61 QTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEK 120 Query: 435 XXXXXXXXXXPASQRFVQARGHIIERF-RASLGCSRQVPEINKTKQAV 575 + ARGH IE L V KTK+AV Sbjct: 121 RYAIASAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAV 168 Score = 67.7 bits (158), Expect = 1e-12 Identities = 31/58 (53%), Positives = 40/58 (68%) Frame = +2 Query: 74 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVRRR 247 ++ ARP VS+Y+ K +V LPFVFKAPIRPDLV VH +++KN RQPY V + Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 117 bits (281), Expect = 2e-27 Identities = 59/108 (54%), Positives = 63/108 (58%), Gaps = 1/108 (0%) Frame = +3 Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434 QTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 61 QTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEK 120 Query: 435 XXXXXXXXXXPASQRFVQARGHIIERF-RASLGCSRQVPEINKTKQAV 575 + ARGH IE L V KTK+AV Sbjct: 121 RYAISSAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAV 168 Score = 68.5 bits (160), Expect = 7e-13 Identities = 32/58 (55%), Positives = 40/58 (68%) Frame = +2 Query: 74 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVRRR 247 ++ ARP VS+YS K +V LPFVFKAPIRPDLV VH +++KN RQPY V + Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57 >SPAC12G12.07c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 27.1 bits (57), Expect = 2.2 Identities = 18/47 (38%), Positives = 22/47 (46%) Frame = +3 Query: 216 TRGSPTA*EGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGN 356 TRGS SQ S + GR R R RGG +RS +G + N Sbjct: 345 TRGSSKRPSVNRSQGSGQRGRGGRGFYRGGRGRGGFFNRSKRGQYSN 391 >SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 26.2 bits (55), Expect = 3.9 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = -1 Query: 135 APCTVSLFSEYTDTKGRATDRLISLL 58 A C V ++SEYT G ++ L++L+ Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515 >SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltransferase Pis1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 25.8 bits (54), Expect = 5.2 Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%) Frame = -2 Query: 92 KVG-LPIDSFRYFSEAIPLKY-TLIAVI 15 K+G LP+ SF Y + +PL Y TL+AV+ Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213 >SPAC3C7.07c |||arginine-tRNA protein transferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 361 Score = 25.0 bits (52), Expect = 9.0 Identities = 9/22 (40%), Positives = 15/22 (68%), Gaps = 2/22 (9%) Frame = -1 Query: 312 GHAEFGRQHV--QYPMIQHWFV 253 G++ F +HV +P ++HWFV Sbjct: 320 GNSTFPTRHVLSYFPQVKHWFV 341 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,346,335 Number of Sequences: 5004 Number of extensions: 43279 Number of successful extensions: 126 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 120 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 124 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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