BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060763.seq (520 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g01600.1 68414.m00077 cytochrome P450, putative similar to cy... 33 0.087 At1g61080.1 68414.m06877 proline-rich family protein 32 0.27 At1g02460.1 68414.m00195 glycoside hydrolase family 28 protein /... 31 0.61 At3g05090.2 68416.m00553 transducin family protein / WD-40 repea... 30 0.81 At3g05090.1 68416.m00552 transducin family protein / WD-40 repea... 30 0.81 At5g07040.1 68418.m00797 zinc finger (C3HC4-type RING finger) fa... 28 3.3 At3g10810.1 68416.m01301 zinc finger (C3HC4-type RING finger) fa... 28 4.3 At2g13270.1 68415.m01462 hypothetical protein 28 4.3 At4g36390.1 68417.m05170 radical SAM domain-containing protein /... 27 5.7 At4g00120.1 68417.m00012 basic helix-loop-helix (bHLH) family pr... 27 5.7 >At1g01600.1 68414.m00077 cytochrome P450, putative similar to cytochrome P450 GI:10442763 from [Triticum aestivum] Length = 554 Score = 33.5 bits (73), Expect = 0.087 Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 2/68 (2%) Frame = +3 Query: 39 KVCSVATMSAWTXXMLSWRDERLGLTELPSTTTRRKHRSAPYRLHRPHLP--SAHVSEPP 212 ++CSV + T + SW +E LG E+ + S RL+ P +P S HV Sbjct: 337 EICSVLIETRGTDDVASWTEEPLGFDEIDRLVYLKAAISETLRLY-PSVPEDSKHVENDD 395 Query: 213 TEPXGTHI 236 P GT + Sbjct: 396 VLPDGTFV 403 >At1g61080.1 68414.m06877 proline-rich family protein Length = 907 Score = 31.9 bits (69), Expect = 0.27 Identities = 25/69 (36%), Positives = 36/69 (52%) Frame = -2 Query: 468 SSK*QILVIFXTPRLAKDVKQYRKQVSVDAEPASTPRPNRSSNQLRPSTREQTCAARPLS 289 SSK Q + +P+++K KQ K S +P+S+P P RSS + S T + R S Sbjct: 8 SSKLQPVKGQASPKVSKSSKQDVKTSSSSPKPSSSPIPIRSSKSIL-SGSYLTSSRRVSS 66 Query: 288 RTGSLSXIS 262 G+L IS Sbjct: 67 PIGNLKNIS 75 >At1g02460.1 68414.m00195 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein similar to polygalacturonase PG1 GI:5669846, PG2 GI:5669848 from (Glycine max); contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 491 Score = 30.7 bits (66), Expect = 0.61 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +3 Query: 138 RRKHRSAPYRLHRPHLPSAHVSEPPTEPXG 227 + KH + + +P PS+ +S+PPT P G Sbjct: 35 KHKHNNHNHHSSKPKPPSSSISQPPTPPPG 64 >At3g05090.2 68416.m00553 transducin family protein / WD-40 repeat family protein contains seven G-protein beta WD-40 repeats; similar to uncharacterized KIAA1449 protein (gi:7959157) [Homo sapiens] Length = 753 Score = 30.3 bits (65), Expect = 0.81 Identities = 22/87 (25%), Positives = 37/87 (42%) Frame = +1 Query: 118 SYRARRRGENIVARLTDYTGRICLPLTCPSPRLSLXELTSRGCIQXVGAYX*KTTGARQG 297 S + R +N+ L D TGR CL + S + L +L + C+ + Sbjct: 248 SMKLRGHTDNVRVLLLDSTGRFCLSGSSDS-MIRLWDLGQQRCLHTYAVHTDSVWALACN 306 Query: 298 PSCTSLFARGGTQLIXRSVWAWRRSRL 378 PS + +++ G Q + + A R S L Sbjct: 307 PSFSHVYSGGRDQCLYLTDLATRESVL 333 >At3g05090.1 68416.m00552 transducin family protein / WD-40 repeat family protein contains seven G-protein beta WD-40 repeats; similar to uncharacterized KIAA1449 protein (gi:7959157) [Homo sapiens] Length = 753 Score = 30.3 bits (65), Expect = 0.81 Identities = 22/87 (25%), Positives = 37/87 (42%) Frame = +1 Query: 118 SYRARRRGENIVARLTDYTGRICLPLTCPSPRLSLXELTSRGCIQXVGAYX*KTTGARQG 297 S + R +N+ L D TGR CL + S + L +L + C+ + Sbjct: 248 SMKLRGHTDNVRVLLLDSTGRFCLSGSSDS-MIRLWDLGQQRCLHTYAVHTDSVWALACN 306 Query: 298 PSCTSLFARGGTQLIXRSVWAWRRSRL 378 PS + +++ G Q + + A R S L Sbjct: 307 PSFSHVYSGGRDQCLYLTDLATRESVL 333 >At5g07040.1 68418.m00797 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 159 Score = 28.3 bits (60), Expect = 3.3 Identities = 12/22 (54%), Positives = 14/22 (63%) Frame = +2 Query: 8 RKPVRCIVTC*SVQCRHNECVD 73 R+PVRCI C C H +CVD Sbjct: 104 REPVRCIPEC--NHCFHTDCVD 123 >At3g10810.1 68416.m01301 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 Zinc finger, C3HC4 type (RING finger) Length = 496 Score = 27.9 bits (59), Expect = 4.3 Identities = 13/42 (30%), Positives = 20/42 (47%), Gaps = 1/42 (2%) Frame = +3 Query: 123 PSTTTRRKHRSAPYRLHRPHLPSAHVSEPP-TEPXGTHISRL 245 P+ + K P PH+ AH ++PP TEP H + + Sbjct: 422 PAPISAAKSHIVPISAPLPHVVFAHAAQPPITEPREPHANEV 463 >At2g13270.1 68415.m01462 hypothetical protein Length = 378 Score = 27.9 bits (59), Expect = 4.3 Identities = 15/38 (39%), Positives = 20/38 (52%) Frame = +3 Query: 108 GLTELPSTTTRRKHRSAPYRLHRPHLPSAHVSEPPTEP 221 G+T + S+TT H +AP LH P + S PP P Sbjct: 248 GITAISSSTTVTSH-NAPLILHPPPKRLRYSSPPPKPP 284 >At4g36390.1 68417.m05170 radical SAM domain-containing protein / TRAM domain-containing protein similar to CDK5 activator-binding protein [Rattus norvegicus] GI:7330738; contains Pfam profiles PF00919: UPF0004 family protein, PF01938: TRAM domain, PF04055: radical SAM domain protein Length = 640 Score = 27.5 bits (58), Expect = 5.7 Identities = 18/62 (29%), Positives = 30/62 (48%) Frame = -1 Query: 418 GCKTVQEAGLRRCRAGFYATPKQIFXSAASLHARTNLCSSAPVSHR*SFKXKRQXSGYSL 239 GC +A +RC A + + K S++S A C S+ +HR + K + G+SL Sbjct: 18 GCSLCFKASTQRCFALRFLSSKATHASSSSSSALLPRCRSS--THRLTQKPINRKKGFSL 75 Query: 238 EM 233 + Sbjct: 76 NL 77 >At4g00120.1 68417.m00012 basic helix-loop-helix (bHLH) family protein contains Pfam profile: PF00010 helix-loop-helix DNA-binding domain Length = 198 Score = 27.5 bits (58), Expect = 5.7 Identities = 14/30 (46%), Positives = 18/30 (60%) Frame = -2 Query: 387 VDAEPASTPRPNRSSNQLRPSTREQTCAAR 298 VD +PA+ P+PNR +R S QT AR Sbjct: 101 VDIDPATVPKPNR--RNVRISDDPQTVVAR 128 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,614,667 Number of Sequences: 28952 Number of extensions: 205574 Number of successful extensions: 533 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 518 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 532 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 947539968 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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