BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060750.seq (641 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g02590.1 68416.m00250 delta 7-sterol-C5-desaturase, putative ... 29 3.5 At3g02580.1 68416.m00249 delta 7-sterol-C5-desaturase (STE1) ide... 28 4.6 >At3g02590.1 68416.m00250 delta 7-sterol-C5-desaturase, putative similar to delta7 sterol C-5 desaturase GI:5031219 from [Arabidopsis thaliana] Length = 279 Score = 28.7 bits (61), Expect = 3.5 Identities = 13/48 (27%), Positives = 24/48 (50%) Frame = +3 Query: 264 NRSKLKYIIF*SCWRRRPNFISTFILIFWTS*SLYFNFTRIWYNFSYY 407 NR L +++ + W P+F+ T++ + LYF +W + YY Sbjct: 20 NRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67 >At3g02580.1 68416.m00249 delta 7-sterol-C5-desaturase (STE1) identical to sterol-C5-desaturase GB:AAD12944 GI:4234768 from [Arabidopsis thaliana] Length = 281 Score = 28.3 bits (60), Expect = 4.6 Identities = 13/48 (27%), Positives = 24/48 (50%) Frame = +3 Query: 264 NRSKLKYIIF*SCWRRRPNFISTFILIFWTS*SLYFNFTRIWYNFSYY 407 NR L +++ + W P+F+ T++ + LYF +W + YY Sbjct: 19 NRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLWCFYIYY 66 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,991,005 Number of Sequences: 28952 Number of extensions: 159280 Number of successful extensions: 264 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 261 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 264 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1324661040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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