BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060748.seq (670 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g28650.1 68416.m03576 DC1 domain-containing protein similar ... 29 2.8 At3g05750.1 68416.m00646 expressed protein 29 2.8 At2g43930.1 68415.m05460 protein kinase family protein contains ... 29 3.7 At5g24550.1 68418.m02899 glycosyl hydrolase family 1 protein con... 28 6.5 At3g45190.1 68416.m04877 SIT4 phosphatase-associated family prot... 28 6.5 At3g27510.1 68416.m03439 DC1 domain-containing protein contains ... 28 6.5 At3g19470.1 68416.m02468 F-box family protein contains F-box dom... 28 6.5 At5g24540.1 68418.m02898 glycosyl hydrolase family 1 protein con... 27 8.5 At5g10060.1 68418.m01165 expressed protein 27 8.5 At3g22770.1 68416.m02871 F-box family protein-related contains T... 27 8.5 >At3g28650.1 68416.m03576 DC1 domain-containing protein similar to hypothetical protein GI:4204272 from [Arabidopsis thaliana] contains weak PHD zinc finger motifs contains weak PHD zinc finger motifs DC1 domain, a divergent protein kinase C domain of unknown function. Length = 665 Score = 29.1 bits (62), Expect = 2.8 Identities = 15/36 (41%), Positives = 20/36 (55%), Gaps = 4/36 (11%) Frame = -3 Query: 620 NNSNRPLAHIC*C-IPGSVLLKKFSK---LIYFCMT 525 N S+RPL H C C PG +LK F + Y+C + Sbjct: 609 NRSSRPLCHNCHCHCPGPFILKDFVEECFCSYYCFS 644 >At3g05750.1 68416.m00646 expressed protein Length = 798 Score = 29.1 bits (62), Expect = 2.8 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 1/55 (1%) Frame = +2 Query: 311 EYVNNFQQFLNT-IRNNFNGPCAKHDMGSSCEDTEEATEKQAVQQTLDGHDWVLE 472 E VN+F N I + + +++D + E+TE +E A+ + +GHDW LE Sbjct: 592 EEVNSFSTAENLQISCSTSFSSSRNDYHHNIEETE-LSESVALSEAEEGHDWELE 645 >At2g43930.1 68415.m05460 protein kinase family protein contains similarity to NPK1-related protein kinase 2 GI:2342425 from [Arabidopsis thaliana] Length = 204 Score = 28.7 bits (61), Expect = 3.7 Identities = 13/28 (46%), Positives = 18/28 (64%) Frame = -1 Query: 127 NHHPFLHRIKTLIKDFNNTLLFGAYIQI 44 +HH LH IK LI++ + TLLF + I Sbjct: 2 DHHRRLHTIKILIRNSSTTLLFFKIVPI 29 >At5g24550.1 68418.m02899 glycosyl hydrolase family 1 protein contains Pfam PF00232 : Glycosyl hydrolase family 1 domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase; similar to anther-specific protein ATA27 (GI:2746341) [Arabidopsis thaliana] Length = 534 Score = 27.9 bits (59), Expect = 6.5 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +2 Query: 275 IRNTRTGTRRLFEYVNNFQQFLNTIRNNFNGP 370 I + GT+ L+ Y ++ LN I+N +N P Sbjct: 380 ISSESDGTKILWSYPEGLRKLLNYIKNKYNNP 411 >At3g45190.1 68416.m04877 SIT4 phosphatase-associated family protein contains Pfam profile: PF04499 SIT4 phosphatase-associated protein Length = 830 Score = 27.9 bits (59), Expect = 6.5 Identities = 24/88 (27%), Positives = 40/88 (45%), Gaps = 3/88 (3%) Frame = +2 Query: 254 EDANHLIIRNTRTGTRRLFEYVNNFQQFLNTIRNNFNGPCAKHDMGSSCEDTEEATEKQA 433 +++ ++I + R G E NN F N+ F G + G+ +EEA E + Sbjct: 599 DESAEVVISSLRLGD----EQANNL--FTNSNWFTFQGDELGENTGTGAISSEEAMEDVS 652 Query: 434 VQQTLDGHDWVLESNDFCIFVK---PFI 508 + +T DG D E D C+ + PF+ Sbjct: 653 LNETSDGGDEEDEDED-CLITESKNPFV 679 >At3g27510.1 68416.m03439 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 566 Score = 27.9 bits (59), Expect = 6.5 Identities = 12/22 (54%), Positives = 16/22 (72%), Gaps = 1/22 (4%) Frame = -3 Query: 620 NNSNRPLAHIC*C-IPGSVLLK 558 N+S+RPL H C C PGS +L+ Sbjct: 520 NSSSRPLCHNCHCRCPGSFILR 541 >At3g19470.1 68416.m02468 F-box family protein contains F-box domain Pfam:PF00646 Length = 382 Score = 27.9 bits (59), Expect = 6.5 Identities = 15/37 (40%), Positives = 21/37 (56%) Frame = -3 Query: 194 VVNHFGYRVYIFRVNLFNNFDQKSPSIFT*NKNANKR 84 VV +RVY+ RVNL NN + S S + + + KR Sbjct: 59 VVLMMNFRVYLMRVNLQNNVVESSSSSSSSSSSCIKR 95 >At5g24540.1 68418.m02898 glycosyl hydrolase family 1 protein contains Pfam PF00232 : Glycosyl hydrolase family 1 domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase; similar to amygdalin hydrolase isoform AH I precursor (GI:16757966) [Prunus serotina] Length = 534 Score = 27.5 bits (58), Expect = 8.5 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = +2 Query: 293 GTRRLFEYVNNFQQFLNTIRNNFNGP 370 GT+ L+ Y ++ LN I+N +N P Sbjct: 386 GTKILWSYPEGLRKILNYIKNKYNNP 411 >At5g10060.1 68418.m01165 expressed protein Length = 469 Score = 27.5 bits (58), Expect = 8.5 Identities = 21/72 (29%), Positives = 34/72 (47%), Gaps = 2/72 (2%) Frame = +3 Query: 210 LKDVPEDKTYEINNLKMQI-I*SLETREQEHADCLSTLTIFNSS*TQLETTLTVRAQNTT 386 LKD ++ E++NLK QI + +T E ++ + S T TT+T NT Sbjct: 255 LKDALREQESELDNLKAQIQVAKEQTEEAQNMQKRLNDEDYTSKQTTAATTITETNDNTK 314 Query: 387 WARLAK-TPKKL 419 + +K TP + Sbjct: 315 SGQASKMTPASI 326 >At3g22770.1 68416.m02871 F-box family protein-related contains TIGRFAM TIGR01640: F-box protein interaction domain Length = 327 Score = 27.5 bits (58), Expect = 8.5 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = -3 Query: 194 VVNHFGYRVYIFRVNLFNNFDQKSPS 117 VV +RVY+ RVNL NN + S S Sbjct: 2 VVMMMNFRVYLMRVNLDNNVVESSSS 27 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,044,329 Number of Sequences: 28952 Number of extensions: 274773 Number of successful extensions: 603 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 588 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 603 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1412971776 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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