BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060744.seq (661 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces p... 27 1.8 SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 27 1.8 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.2 SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 26 5.5 SPBPB2B2.10c |||galactose-1-phosphate uridylyltransferase |Schiz... 26 5.5 SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 26 5.5 SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 25 7.3 SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 7.3 SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 9.7 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 9.7 >SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 728 Score = 27.5 bits (58), Expect = 1.8 Identities = 9/27 (33%), Positives = 19/27 (70%) Frame = +1 Query: 313 LITKSGVIQLIMKSKLPYAIELQEWLL 393 L+ K GV+ L+ K KLP+++ ++ ++ Sbjct: 542 LLVKDGVVHLVDKVKLPFSVSQKDMII 568 >SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 349 Score = 27.5 bits (58), Expect = 1.8 Identities = 16/43 (37%), Positives = 23/43 (53%) Frame = +3 Query: 198 STSTASTNRRLSMPTKSSTCSR*RGKAGRPAVFAPTHSAHYQI 326 S+S+ S++R S T T S + + RP VF T +HY I Sbjct: 163 SSSSKSSSRSSSRTTSHRTTSH-KSSSYRPTVFPYTTISHYNI 204 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 26.2 bits (55), Expect = 4.2 Identities = 13/28 (46%), Positives = 17/28 (60%) Frame = +3 Query: 177 IVNGQYVSTSTASTNRRLSMPTKSSTCS 260 I G Y STS +ST+ + P+ SST S Sbjct: 129 ISGGIYSSTSASSTSSSTATPSSSSTTS 156 >SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosaccharomyces pombe|chr 2|||Manual Length = 441 Score = 25.8 bits (54), Expect = 5.5 Identities = 16/46 (34%), Positives = 23/46 (50%), Gaps = 4/46 (8%) Frame = +1 Query: 514 DLAEANRSLILF----ANEMIVARRDAETARQDCXNARRETAQWXN 639 DL +ANR L ANE + +RD R+D + +R+ Q N Sbjct: 318 DLLDANRILSTACKKAANEKLAVQRDLSKLREDRLSVQRKKIQLRN 363 >SPBPB2B2.10c |||galactose-1-phosphate uridylyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 369 Score = 25.8 bits (54), Expect = 5.5 Identities = 17/67 (25%), Positives = 31/67 (46%), Gaps = 2/67 (2%) Frame = +2 Query: 353 PNC-LTP*NYK-NGFWRRSFLKCYARSKYAPAVEMDTNDVIAKIDDLTQKLTVATQIWRK 526 P C L P N + GF + Y PAV +D D + ++T+ T+ T++++ Sbjct: 52 PTCYLCPGNIRATGFENPKYETTYVFPNDYPAVRVDQPDYMQDESEITKGNTLKTRMFKT 111 Query: 527 QTDRSFC 547 + + C Sbjct: 112 EGVKGKC 118 >SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 25.8 bits (54), Expect = 5.5 Identities = 10/31 (32%), Positives = 18/31 (58%) Frame = +1 Query: 508 HADLAEANRSLILFANEMIVARRDAETARQD 600 H E +FANE+++ + DA++ +QD Sbjct: 544 HKKNPELGNKKSIFANEILIEQADAQSFKQD 574 >SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharomyces pombe|chr 2|||Manual Length = 733 Score = 25.4 bits (53), Expect = 7.3 Identities = 12/38 (31%), Positives = 19/38 (50%) Frame = -3 Query: 587 VSASRRATIISLANKMSDRFASAKSAWPPSIFASDRQF 474 + S+ + +S N++S +PPS F S RQF Sbjct: 198 LQTSQDSPSLSATNQLSSSDTLEPLQYPPSSFGSQRQF 235 >SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 669 Score = 25.4 bits (53), Expect = 7.3 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +2 Query: 116 GDEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHAD 241 GDE V+ D S V+ + ++ GKY+ TF H D Sbjct: 48 GDEIECIVVSMDDKSKKARVSLRQEDILNALGKYEETFRHVD 89 >SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 25.0 bits (52), Expect = 9.7 Identities = 8/14 (57%), Positives = 11/14 (78%) Frame = -3 Query: 197 HVLPVHNLHILNCW 156 HVLP+ L +LNC+ Sbjct: 405 HVLPIRQLFLLNCY 418 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 25.0 bits (52), Expect = 9.7 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = -3 Query: 239 RHAQTSICTCRRRGHVLPVHNLHILN 162 R A T + R +LPVH LH+ + Sbjct: 776 RQAMTRLKGLRETNSILPVHQLHMFH 801 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,747,370 Number of Sequences: 5004 Number of extensions: 57411 Number of successful extensions: 150 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 146 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 150 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 299817502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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