Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060741.seq
(686 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g62010.1 68416.m06964 expressed protein 31 0.72
At1g01440.1 68414.m00059 extra-large G-protein-related weak simi... 31 0.72
At2g11620.1 68415.m01249 hypothetical protein 31 0.95
At1g27430.1 68414.m03343 GYF domain-containing protein contains ... 29 2.9
At4g20370.1 68417.m02973 twin sister of FT protein (TSF) / TFL1 ... 29 3.8
At5g43420.1 68418.m05309 zinc finger (C3HC4-type RING finger) fa... 28 5.0
At3g27730.1 68416.m03462 ATP-dependent DNA helicase, putative si... 28 5.0
At5g55690.1 68418.m06943 MADS-box protein (AGL47) 28 6.7
At4g12080.1 68417.m01920 DNA-binding family protein contains a A... 28 6.7
At2g27920.2 68415.m03383 serine carboxypeptidase S10 family prot... 28 6.7
At2g27920.1 68415.m03384 serine carboxypeptidase S10 family prot... 28 6.7
At5g57070.1 68418.m07124 hydroxyproline-rich glycoprotein family... 27 8.8
At4g27850.1 68417.m03999 proline-rich family protein contains pr... 27 8.8
At4g00890.1 68417.m00120 proline-rich family protein contains pr... 27 8.8
>At3g62010.1 68416.m06964 expressed protein
Length = 1254
Score = 31.1 bits (67), Expect = 0.72
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = +3
Query: 300 QLEQLDAMLDKELALEGRAYGNDALVADE 386
++EQ++A L +++ +E YGN LV DE
Sbjct: 124 RVEQMEARLKEDILMEASRYGNKILVTDE 152
>At1g01440.1 68414.m00059 extra-large G-protein-related weak
similarity to extra-large G-protein (AtXLG1)
(GI:3201682) [Arabidopsis thaliana]
Length = 664
Score = 31.1 bits (67), Expect = 0.72
Identities = 21/51 (41%), Positives = 28/51 (54%)
Frame = +3
Query: 303 LEQLDAMLDKELALEGRAYGNDALVADEPLPLANAHALHGVPPMLSSVLPE 455
L +LD++ D L R GN+A V D+P P+ HA HG PP + PE
Sbjct: 89 LRKLDSIKDHLL----RGGGNNATVVDQP-PMG-FHAHHGPPPSYYNPYPE 133
>At2g11620.1 68415.m01249 hypothetical protein
Length = 491
Score = 30.7 bits (66), Expect = 0.95
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +1
Query: 70 VMEKEHQPDSMATITMKPEYPPSEVYSTSEPPPAYRHRVS 189
V+ + + M + MKPE PP ++Y +P P + S
Sbjct: 253 VLRYDGNNNGMQGVLMKPEKPPQDLYGQCQPQPQIQRNFS 292
>At1g27430.1 68414.m03343 GYF domain-containing protein contains Pfam
profile: PF02213 GYF domain
Length = 1531
Score = 29.1 bits (62), Expect = 2.9
Identities = 9/21 (42%), Positives = 16/21 (76%)
Frame = -2
Query: 499 SSLKRLGREDDGWLVSGNTED 437
S ++RLG + + W+V G+T+D
Sbjct: 1148 SEIRRLGEDPNSWMVGGSTDD 1168
>At4g20370.1 68417.m02973 twin sister of FT protein (TSF) / TFL1
like protein identical to SP|Q9S7R5 TWIN SISTER of FT
protein (TFL1 like protein) {Arabidopsis thaliana};
contains Pfam profile PF01161:
Phosphatidylethanolamine-binding protein
Length = 175
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/47 (29%), Positives = 24/47 (51%)
Frame = +3
Query: 276 VAARSSCHQLEQLDAMLDKELALEGRAYGNDALVADEPLPLANAHAL 416
V + S+ HQ E L ++ A G A+GN+ + + P P + H +
Sbjct: 74 VPSPSNPHQREYLHWLVTDIPATTGNAFGNEVVCYESPRPPSGIHRI 120
>At5g43420.1 68418.m05309 zinc finger (C3HC4-type RING finger)
family protein low similarity to RING-H2 zinc finger
protein ATL4 [Arabidopsis thaliana] GI:4928399; contains
Pfam profile PF00097: Zinc finger, C3HC4 type (RING
finger)
Length = 375
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
Frame = +1
Query: 160 PPPAYRHRVS---TSVQIAKIAALTVVASSFILGTLY 261
PPP HR S TS I +A + ++A++F+L + Y
Sbjct: 19 PPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYY 55
>At3g27730.1 68416.m03462 ATP-dependent DNA helicase, putative
similar to SP|P51979 ATP-dependent DNA helicase MER3 (EC
3.6.1.-) {Saccharomyces cerevisiae}; contains Pfam
profiles PF00270: DEAD/DEAH box helicase, PF02889: Sec63
domain
Length = 1113
Score = 28.3 bits (60), Expect = 5.0
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = +3
Query: 426 PPMLSSVLPETSQPSSSRPSLFKDDALNMPSPK 524
PPM S+ + + P +S PS KD +MPS K
Sbjct: 859 PPMASACIADDDNPVTSGPSNRKDKKDDMPSFK 891
>At5g55690.1 68418.m06943 MADS-box protein (AGL47)
Length = 277
Score = 27.9 bits (59), Expect = 6.7
Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +3
Query: 534 TNCRKLTTIRMTHQTRVMRVLSPTVLLKRTTNLKRLDRCS-NCLFSL 671
++C K T++ + +V PT+ K T K+LD+CS N L+++
Sbjct: 84 SSCTKTYTVQECLEKNNTKVEKPTIATKYPTWDKKLDQCSLNDLYAV 130
>At4g12080.1 68417.m01920 DNA-binding family protein contains a AT
hook motif (DNA binding motifs with a preference for A/T
rich regions), Pfam:PF02178
Length = 356
Score = 27.9 bits (59), Expect = 6.7
Identities = 21/74 (28%), Positives = 35/74 (47%), Gaps = 1/74 (1%)
Frame = +3
Query: 261 LASSWVAARSSCHQLEQLDAMLDKELALEGRAYGNDA-LVADEPLPLANAHALHGVPPML 437
L S V ++ +E + L K+ R YG D +VA P P+++A A +PP
Sbjct: 66 LQISTVTTTTTTAAMEGISGGLMKKKRGRPRKYGPDGTVVALSPKPISSAPAPSHLPPPS 125
Query: 438 SSVLPETSQPSSSR 479
S V+ ++ S+
Sbjct: 126 SHVIDFSASEKRSK 139
>At2g27920.2 68415.m03383 serine carboxypeptidase S10 family protein
similar to retinoid-inducible serine carboxypeptidase
precursor (GI:15146429) [Mus musculus]
Length = 389
Score = 27.9 bits (59), Expect = 6.7
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +3
Query: 216 STNSGRFLLHLGNFILASSWVA 281
+ SG+ LHLG IL SW++
Sbjct: 110 AVQSGKLKLHLGGVILGDSWIS 131
>At2g27920.1 68415.m03384 serine carboxypeptidase S10 family protein
similar to retinoid-inducible serine carboxypeptidase
precursor (GI:15146429) [Mus musculus]
Length = 461
Score = 27.9 bits (59), Expect = 6.7
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +3
Query: 216 STNSGRFLLHLGNFILASSWVA 281
+ SG+ LHLG IL SW++
Sbjct: 182 AVQSGKLKLHLGGVILGDSWIS 203
>At5g57070.1 68418.m07124 hydroxyproline-rich glycoprotein family
protein Common family members: At5g26070, At5g19800,
At1g72790 [Arabidopsis thaliana]
Length = 575
Score = 27.5 bits (58), Expect = 8.8
Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
Frame = +1
Query: 70 VMEKEHQPDSMA--TITMKPEYPPSEVYSTSEPPP 168
+ E+E +P + T +KP PP + +T PPP
Sbjct: 222 IEEEESEPKEIQIDTFVVKPSSPPQQPPATPPPPP 256
>At4g27850.1 68417.m03999 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 577
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/47 (34%), Positives = 17/47 (36%)
Frame = +3
Query: 381 DEPLPLANAHALHGVPPMLSSVLPETSQPSSSRPSLFKDDALNMPSP 521
D PLPL P S LP P S P+ D L P P
Sbjct: 224 DSPLPLPGPPPSSSPTPGPDSPLPSPGPPPSPSPTPGPDSPLPSPGP 270
>At4g00890.1 68417.m00120 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 431
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +3
Query: 393 PLANAHALHGVPPMLSSVLPETS-QPSSSRPSLFKDDALNMPSPK*TKT 536
P+ + + PP SS L +T+ P S P + + + + PSPK T T
Sbjct: 301 PIPSPATITAPPPPFSSPLSQTTPSPKPSLPQI-EPNQIKSPSPKLTNT 348
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,544,012
Number of Sequences: 28952
Number of extensions: 308617
Number of successful extensions: 1421
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 985
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1412
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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