BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060740.seq (680 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 116 2e-27 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 116 2e-27 SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 4.4 SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces ... 26 5.8 SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 26 5.8 SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein Urb1|Schi... 25 7.7 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 116 bits (280), Expect = 2e-27 Identities = 55/97 (56%), Positives = 60/97 (61%) Frame = +1 Query: 241 EQGGCHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXX 420 E+ G HQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 56 EKAG-HQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVK 114 Query: 421 XXXXXXXXXXXXXXXXXXXXXXXQARGHIMKRFPSFP 531 ARGH ++ P P Sbjct: 115 VNQNEKRYAIASAVAASGVPSLLLARGHRIEEIPEVP 151 Score = 75.4 bits (177), Expect = 7e-15 Identities = 34/56 (60%), Positives = 45/56 (80%) Frame = +3 Query: 510 EKIPELPLVVADKVQEXNKTKQAVIFLRRLKAWSDILKVYXSQRLRAGKGKMRNRR 677 E+IPE+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RNRR Sbjct: 145 EEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNRR 200 Score = 71.3 bits (167), Expect = 1e-13 Identities = 33/59 (55%), Positives = 43/59 (72%) Frame = +2 Query: 77 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 253 ++ ARP VS+Y+ K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++A Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKA 58 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 116 bits (280), Expect = 2e-27 Identities = 55/97 (56%), Positives = 60/97 (61%) Frame = +1 Query: 241 EQGGCHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXX 420 E+ G HQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 56 EKAG-HQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVK 114 Query: 421 XXXXXXXXXXXXXXXXXXXXXXXQARGHIMKRFPSFP 531 ARGH ++ P P Sbjct: 115 VNQNEKRYAISSAVAASGVPSLLLARGHRIEEIPEVP 151 Score = 74.9 bits (176), Expect = 1e-14 Identities = 34/56 (60%), Positives = 45/56 (80%) Frame = +3 Query: 510 EKIPELPLVVADKVQEXNKTKQAVIFLRRLKAWSDILKVYXSQRLRAGKGKMRNRR 677 E+IPE+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RNRR Sbjct: 145 EEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNRR 200 Score = 72.1 bits (169), Expect = 7e-14 Identities = 34/59 (57%), Positives = 43/59 (72%) Frame = +2 Query: 77 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 253 ++ ARP VS+YS K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++A Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKA 58 >SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 26.2 bits (55), Expect = 4.4 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = -3 Query: 138 APCTVSLFSEYTDTKGRATDRLISLL 61 A C V ++SEYT G ++ L++L+ Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515 >SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 345 Score = 25.8 bits (54), Expect = 5.8 Identities = 12/41 (29%), Positives = 17/41 (41%) Frame = -3 Query: 522 RESFHNVSSSLNERWDAGSSNGCRQGRSPLSEVDATVPTPP 400 R+S V+ N W + GC G L + A + PP Sbjct: 178 RDSTRQVTIVPNRHWGGNGAIGCGVGHGVLHRLPAPLSGPP 218 >SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltransferase Pis1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 25.8 bits (54), Expect = 5.8 Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%) Frame = -1 Query: 95 KVG-LPIDSFRYFSEAIPLKY-TLIAVI 18 K+G LP+ SF Y + +PL Y TL+AV+ Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213 >SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein Urb1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1568 Score = 25.4 bits (53), Expect = 7.7 Identities = 21/79 (26%), Positives = 37/79 (46%), Gaps = 2/79 (2%) Frame = +2 Query: 77 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPI--RPDLVNDVHVSMSKNSRQPYCVSKE 250 L + R LV K ET+ + P+ + A I RP L+ + ++S + + + + Sbjct: 753 LELIRELVEF--SKEETMATSQSPIRTLLSACINLRPFLLQEQYISFNMDFAEFLIDTNA 810 Query: 251 AVTKPVLNHGVLDVLSPEF 307 ++ V+N VLD L F Sbjct: 811 SIKGSVVNTCVLDFLYKSF 829 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,509,680 Number of Sequences: 5004 Number of extensions: 47005 Number of successful extensions: 129 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 119 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 127 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 313902888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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