BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060737.seq (685 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g09020.1 68414.m01006 protein kinase, putative similar to pro... 51 6e-07 At4g16360.1 68417.m02478 5'-AMP-activated protein kinase beta-2 ... 42 3e-04 At5g21170.1 68418.m02530 5'-AMP-activated protein kinase beta-2 ... 37 0.011 At1g27070.1 68414.m03300 5'-AMP-activated protein kinase-related... 31 0.94 At3g52180.1 68416.m05729 protein tyrosine phosphatase/kinase int... 30 1.2 At1g61940.1 68414.m06987 F-box family protein / tubby family pro... 29 2.9 At4g22770.1 68417.m03287 DNA-binding family protein contains a A... 28 5.0 At2g22460.1 68415.m02663 expressed protein contains Pfam profile... 28 5.0 At3g58950.1 68416.m06569 F-box family protein contains F-box dom... 27 8.8 >At1g09020.1 68414.m01006 protein kinase, putative similar to protein kinase AKINbetagamma-2 [Zea mays] GI:11139548, AKINbetagamma-1 [Zea mays] GI:11139546; contains Pfam profile PF00571: CBS domain Length = 487 Score = 51.2 bits (117), Expect = 6e-07 Identities = 26/55 (47%), Positives = 34/55 (61%), Gaps = 4/55 (7%) Frame = +2 Query: 359 PTVFKWEGGGKQVFISGTFTDW-ETIPMVKSHG---DFVTIIDLPEGEHQYKYFL 511 PT F W GG++VF+SG+FT W E +PM G F I +L G HQYK+F+ Sbjct: 22 PTRFVWPYGGRRVFLSGSFTRWTEHVPMSPLEGCPTVFQVICNLTPGYHQYKFFV 76 Score = 27.9 bits (59), Expect = 6.6 Identities = 10/23 (43%), Positives = 12/23 (52%) Frame = +1 Query: 499 QIFFDGEWRHDPTVKVIDNGNGV 567 + F DGEWRHD + GV Sbjct: 73 KFFVDGEWRHDEHQPFVSGNGGV 95 >At4g16360.1 68417.m02478 5'-AMP-activated protein kinase beta-2 subunit, putative similar to Swiss-Prot:Q9QZH4 5'-AMP-activated protein kinase, beta-2 subunit (AMPK beta-2 chain) [Rattus norvegicus] Length = 259 Score = 42.3 bits (95), Expect = 3e-04 Identities = 16/53 (30%), Positives = 33/53 (62%), Gaps = 1/53 (1%) Frame = +2 Query: 356 LPTVFKWEGGGKQVFISGTFTDWETIPMVKSHG-DFVTIIDLPEGEHQYKYFL 511 +PT+ W GGK++ + G++ +W+T ++ G DF + LP G ++Y++ + Sbjct: 72 IPTMITWCHGGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPSGVYEYRFIV 124 >At5g21170.1 68418.m02530 5'-AMP-activated protein kinase beta-2 subunit, putative similar to Swiss-Prot:Q9QZH4 5'-AMP-activated protein kinase, beta-2 subunit (AMPK beta-2 chain) [Rattus norvegicus] Length = 283 Score = 37.1 bits (82), Expect = 0.011 Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Frame = +2 Query: 356 LPTVFKWEGGGKQVFISGTFTDWETIPMVKSHG-DFVTIIDLPEGEHQYK 502 +PT+ W GG V + G++ +W + ++ G D + LP G + YK Sbjct: 100 IPTIITWNQGGNDVAVEGSWDNWRSRKKLQKSGKDHSILFVLPSGIYHYK 149 >At1g27070.1 68414.m03300 5'-AMP-activated protein kinase-related contains weak similarity to Swiss-Prot:Q9QZH4 5'-AMP-activated protein kinase, beta-2 subunit (AMPK beta-2 chain) [Rattus norvegicus] Length = 532 Score = 30.7 bits (66), Expect = 0.94 Identities = 21/99 (21%), Positives = 46/99 (46%), Gaps = 2/99 (2%) Frame = +2 Query: 299 RERSNTLTDGSKIVDDIKVLPTVFKWEGGGKQVFISGTFTDWETIPMVK--SHGDFVTII 472 RE+ + S+ + ++ V W +V ++G+F W T +K +G F + Sbjct: 436 REKQRRIDHASRALRLLRTASIV--WPNSASEVLLTGSFDGWSTQRKMKKAENGVFSLSL 493 Query: 473 DLPEGEHQYKYFLMENGGMILQ*K*LTMGMGSKNNLVTV 589 L G+++ K F+++ + + + G +NNL+ + Sbjct: 494 KLYPGKYEIK-FIVDGQWKVDPLRPIVTSGGYENNLLII 531 >At3g52180.1 68416.m05729 protein tyrosine phosphatase/kinase interaction sequence protein (PTPKIS1) identical to PTPKIS1 protein [Arabidopsis thaliana] GI:11595504; contains Pfam profile PF00782: Dual specificity phosphatase, catalytic domain Length = 379 Score = 30.3 bits (65), Expect = 1.2 Identities = 17/46 (36%), Positives = 26/46 (56%), Gaps = 3/46 (6%) Frame = +2 Query: 383 GGKQVFISGTFTDW-ETIPMV--KSHGDFVTIIDLPEGEHQYKYFL 511 G +V ISG W + IP+ K G ++ +LPEG+ +YKY + Sbjct: 265 GFSRVEISGLDIGWGQRIPLTLDKGTGFWILKRELPEGQFEYKYII 310 >At1g61940.1 68414.m06987 F-box family protein / tubby family protein similar to putative Tub family protein GI:4309738 from [Arabidopsis thaliana] Length = 265 Score = 29.1 bits (62), Expect = 2.9 Identities = 15/43 (34%), Positives = 22/43 (51%) Frame = -2 Query: 486 PSGKSIIVTKSPCDFTMGIVSQSVNVPEINTCFPPPSHLKTVG 358 PS K+++ C I + VNVPE+++ F P LK G Sbjct: 21 PSRKNVVSCVGVCKNWRQIFKEIVNVPEVSSKFTFPISLKQPG 63 >At4g22770.1 68417.m03287 DNA-binding family protein contains a AT hook motif (DNA binding motifs with a preference for A/T rich regions), Pfam:PF02178 Length = 334 Score = 28.3 bits (60), Expect = 5.0 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = -2 Query: 489 SPSGKSIIVTKSPCDFTMGIVSQSVNVPEINTCFPPP 379 S G +++ + +P DF M S++ N P + PPP Sbjct: 14 SDGGVTVVRSNAPSDFHMAPRSETSNTPPNSVAPPPP 50 >At2g22460.1 68415.m02663 expressed protein contains Pfam profile PF04759: Protein of unknown function, DUF617 Length = 245 Score = 28.3 bits (60), Expect = 5.0 Identities = 15/60 (25%), Positives = 27/60 (45%) Frame = +2 Query: 233 YRRWRSLYTKAVPESDIEYTEQRERSNTLTDGSKIVDDIKVLPTVFKWEGGGKQVFISGT 412 +R RS +P + ++ E+ NT G K+ +DIK + F+ ++GT Sbjct: 29 WRLLRSFIQLLIPTCNCTLVQELEQDNTNLRGKKLYNDIKSRTSSFRSSSSLSSSTVTGT 88 >At3g58950.1 68416.m06569 F-box family protein contains F-box domain Pfam:PF00646 Length = 417 Score = 27.5 bits (58), Expect = 8.8 Identities = 14/41 (34%), Positives = 21/41 (51%) Frame = +2 Query: 236 RRWRSLYTKAVPESDIEYTEQRERSNTLTDGSKIVDDIKVL 358 +RWR+ + VP DI+ +RER L VD++ L Sbjct: 32 KRWRNQFA-LVPNLDIDEEGKREREEILLSFMDFVDNVLAL 71 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,187,331 Number of Sequences: 28952 Number of extensions: 333535 Number of successful extensions: 787 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 768 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 784 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1447936096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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