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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060737.seq
         (685 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g09020.1 68414.m01006 protein kinase, putative similar to pro...    51   6e-07
At4g16360.1 68417.m02478 5'-AMP-activated protein kinase beta-2 ...    42   3e-04
At5g21170.1 68418.m02530 5'-AMP-activated protein kinase beta-2 ...    37   0.011
At1g27070.1 68414.m03300 5'-AMP-activated protein kinase-related...    31   0.94 
At3g52180.1 68416.m05729 protein tyrosine phosphatase/kinase int...    30   1.2  
At1g61940.1 68414.m06987 F-box family protein / tubby family pro...    29   2.9  
At4g22770.1 68417.m03287 DNA-binding family protein contains a A...    28   5.0  
At2g22460.1 68415.m02663 expressed protein contains Pfam profile...    28   5.0  
At3g58950.1 68416.m06569 F-box family protein contains F-box dom...    27   8.8  

>At1g09020.1 68414.m01006 protein kinase, putative similar to
           protein kinase AKINbetagamma-2 [Zea mays] GI:11139548,
           AKINbetagamma-1 [Zea mays] GI:11139546; contains Pfam
           profile PF00571: CBS domain
          Length = 487

 Score = 51.2 bits (117), Expect = 6e-07
 Identities = 26/55 (47%), Positives = 34/55 (61%), Gaps = 4/55 (7%)
 Frame = +2

Query: 359 PTVFKWEGGGKQVFISGTFTDW-ETIPMVKSHG---DFVTIIDLPEGEHQYKYFL 511
           PT F W  GG++VF+SG+FT W E +PM    G    F  I +L  G HQYK+F+
Sbjct: 22  PTRFVWPYGGRRVFLSGSFTRWTEHVPMSPLEGCPTVFQVICNLTPGYHQYKFFV 76



 Score = 27.9 bits (59), Expect = 6.6
 Identities = 10/23 (43%), Positives = 12/23 (52%)
 Frame = +1

Query: 499 QIFFDGEWRHDPTVKVIDNGNGV 567
           + F DGEWRHD     +    GV
Sbjct: 73  KFFVDGEWRHDEHQPFVSGNGGV 95


>At4g16360.1 68417.m02478 5'-AMP-activated protein kinase beta-2
           subunit, putative similar to Swiss-Prot:Q9QZH4
           5'-AMP-activated protein kinase, beta-2 subunit (AMPK
           beta-2 chain) [Rattus norvegicus]
          Length = 259

 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 16/53 (30%), Positives = 33/53 (62%), Gaps = 1/53 (1%)
 Frame = +2

Query: 356 LPTVFKWEGGGKQVFISGTFTDWETIPMVKSHG-DFVTIIDLPEGEHQYKYFL 511
           +PT+  W  GGK++ + G++ +W+T   ++  G DF  +  LP G ++Y++ +
Sbjct: 72  IPTMITWCHGGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPSGVYEYRFIV 124


>At5g21170.1 68418.m02530 5'-AMP-activated protein kinase beta-2
           subunit, putative similar to Swiss-Prot:Q9QZH4
           5'-AMP-activated protein kinase, beta-2 subunit (AMPK
           beta-2 chain) [Rattus norvegicus]
          Length = 283

 Score = 37.1 bits (82), Expect = 0.011
 Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
 Frame = +2

Query: 356 LPTVFKWEGGGKQVFISGTFTDWETIPMVKSHG-DFVTIIDLPEGEHQYK 502
           +PT+  W  GG  V + G++ +W +   ++  G D   +  LP G + YK
Sbjct: 100 IPTIITWNQGGNDVAVEGSWDNWRSRKKLQKSGKDHSILFVLPSGIYHYK 149


>At1g27070.1 68414.m03300 5'-AMP-activated protein kinase-related
           contains weak similarity to Swiss-Prot:Q9QZH4
           5'-AMP-activated protein kinase, beta-2 subunit (AMPK
           beta-2 chain) [Rattus norvegicus]
          Length = 532

 Score = 30.7 bits (66), Expect = 0.94
 Identities = 21/99 (21%), Positives = 46/99 (46%), Gaps = 2/99 (2%)
 Frame = +2

Query: 299 RERSNTLTDGSKIVDDIKVLPTVFKWEGGGKQVFISGTFTDWETIPMVK--SHGDFVTII 472
           RE+   +   S+ +  ++    V  W     +V ++G+F  W T   +K   +G F   +
Sbjct: 436 REKQRRIDHASRALRLLRTASIV--WPNSASEVLLTGSFDGWSTQRKMKKAENGVFSLSL 493

Query: 473 DLPEGEHQYKYFLMENGGMILQ*K*LTMGMGSKNNLVTV 589
            L  G+++ K F+++    +   + +    G +NNL+ +
Sbjct: 494 KLYPGKYEIK-FIVDGQWKVDPLRPIVTSGGYENNLLII 531


>At3g52180.1 68416.m05729 protein tyrosine phosphatase/kinase
           interaction sequence protein (PTPKIS1) identical to
           PTPKIS1 protein [Arabidopsis thaliana] GI:11595504;
           contains Pfam profile PF00782: Dual specificity
           phosphatase, catalytic domain
          Length = 379

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 17/46 (36%), Positives = 26/46 (56%), Gaps = 3/46 (6%)
 Frame = +2

Query: 383 GGKQVFISGTFTDW-ETIPMV--KSHGDFVTIIDLPEGEHQYKYFL 511
           G  +V ISG    W + IP+   K  G ++   +LPEG+ +YKY +
Sbjct: 265 GFSRVEISGLDIGWGQRIPLTLDKGTGFWILKRELPEGQFEYKYII 310


>At1g61940.1 68414.m06987 F-box family protein / tubby family
           protein similar to putative Tub family protein
           GI:4309738 from [Arabidopsis thaliana]
          Length = 265

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 15/43 (34%), Positives = 22/43 (51%)
 Frame = -2

Query: 486 PSGKSIIVTKSPCDFTMGIVSQSVNVPEINTCFPPPSHLKTVG 358
           PS K+++     C     I  + VNVPE+++ F  P  LK  G
Sbjct: 21  PSRKNVVSCVGVCKNWRQIFKEIVNVPEVSSKFTFPISLKQPG 63


>At4g22770.1 68417.m03287 DNA-binding family protein contains a AT
           hook motif (DNA binding motifs with a preference for A/T
           rich regions), Pfam:PF02178
          Length = 334

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 12/37 (32%), Positives = 20/37 (54%)
 Frame = -2

Query: 489 SPSGKSIIVTKSPCDFTMGIVSQSVNVPEINTCFPPP 379
           S  G +++ + +P DF M   S++ N P  +   PPP
Sbjct: 14  SDGGVTVVRSNAPSDFHMAPRSETSNTPPNSVAPPPP 50


>At2g22460.1 68415.m02663 expressed protein contains Pfam profile
           PF04759: Protein of unknown function, DUF617
          Length = 245

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 15/60 (25%), Positives = 27/60 (45%)
 Frame = +2

Query: 233 YRRWRSLYTKAVPESDIEYTEQRERSNTLTDGSKIVDDIKVLPTVFKWEGGGKQVFISGT 412
           +R  RS     +P  +    ++ E+ NT   G K+ +DIK   + F+         ++GT
Sbjct: 29  WRLLRSFIQLLIPTCNCTLVQELEQDNTNLRGKKLYNDIKSRTSSFRSSSSLSSSTVTGT 88


>At3g58950.1 68416.m06569 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 417

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 14/41 (34%), Positives = 21/41 (51%)
 Frame = +2

Query: 236 RRWRSLYTKAVPESDIEYTEQRERSNTLTDGSKIVDDIKVL 358
           +RWR+ +   VP  DI+   +RER   L      VD++  L
Sbjct: 32  KRWRNQFA-LVPNLDIDEEGKREREEILLSFMDFVDNVLAL 71


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,187,331
Number of Sequences: 28952
Number of extensions: 333535
Number of successful extensions: 787
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 768
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 784
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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