BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060731.seq
(640 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AB047034-1|BAB64310.1| 1598|Apis mellifera mblk-1 protein. 29 0.029
M29492-1|AAA27727.1| 74|Apis mellifera protein ( Bee homeobox-... 25 0.82
AF000632-1|AAC61894.1| 452|Apis mellifera major royal jelly pro... 25 0.82
AY661557-1|AAT74557.1| 411|Apis mellifera yellow-f-like protein... 24 1.4
DQ667192-1|ABG75744.1| 489|Apis mellifera pH-sensitive chloride... 23 1.9
DQ667191-1|ABG75743.1| 475|Apis mellifera pH-sensitive chloride... 23 1.9
DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride... 23 1.9
DQ667189-1|ABG75741.1| 458|Apis mellifera pH-sensitive chloride... 23 1.9
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 22 4.4
>AB047034-1|BAB64310.1| 1598|Apis mellifera mblk-1 protein.
Length = 1598
Score = 29.5 bits (63), Expect = 0.029
Identities = 22/71 (30%), Positives = 31/71 (43%), Gaps = 1/71 (1%)
Frame = -2
Query: 219 TMSNQPQQQNKFKFGIPASKQIKLPQTQQFGYRPQNQTHFGYRPPLNQNQFGYKPNL-NQ 43
T QPQQQ + + +Q + PQ QQ + Q Q +P Q ++G L Q
Sbjct: 1500 TQQQQPQQQQQQQQQQQPQQQSQQPQQQQPQPQQQQQQQQQQQPQQQQKEYGAVSGLVVQ 1559
Query: 42 PQFGYRPQLNQ 10
Q GY N+
Sbjct: 1560 LQRGYNSGNNR 1570
Score = 23.4 bits (48), Expect = 1.9
Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 5/53 (9%)
Frame = -2
Query: 231 QNVATMSNQP-----QQQNKFKFGIPASKQIKLPQTQQFGYRPQNQTHFGYRP 88
Q+ AT ++QP + +N I +Q K+P G P++ + GYRP
Sbjct: 503 QDSATPADQPLDLSAKPKNSQDNNISLLEQQKIPLRMTAGIDPKSIFNSGYRP 555
Score = 21.4 bits (43), Expect = 7.6
Identities = 21/69 (30%), Positives = 26/69 (37%), Gaps = 4/69 (5%)
Frame = -2
Query: 231 QNVATMSNQPQQQNKFKFGIPASKQIKLPQTQQFGYRPQN----QTHFGYRPPLNQNQFG 64
Q S QPQQQ +Q + PQ QQ Y + Q GY N+ G
Sbjct: 1515 QQPQQQSQQPQQQQPQPQQQQQQQQQQQPQQQQKEYGAVSGLVVQLQRGYNS--GNNRSG 1572
Query: 63 YKPNLNQPQ 37
+ N Q Q
Sbjct: 1573 EQANSQQQQ 1581
>M29492-1|AAA27727.1| 74|Apis mellifera protein ( Bee
homeobox-containing gene,partial cds, clone H40. ).
Length = 74
Score = 24.6 bits (51), Expect = 0.82
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = -3
Query: 452 LKGCDSLEKALSIVLEEVNFMFQYSSRNKMLKQQN 348
L C+ L ALS+ L E + +R K+QN
Sbjct: 33 LSVCERLNLALSLSLTETQVKIWFQNRRTKWKKQN 67
>AF000632-1|AAC61894.1| 452|Apis mellifera major royal jelly
protein MRJP2 protein.
Length = 452
Score = 24.6 bits (51), Expect = 0.82
Identities = 16/61 (26%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Frame = -3
Query: 461 IVRLKGCDSLEKALSIVLEEVNFMFQY-SSRNKMLKQQNTQCTAPRPQPFNNFQFSDKNV 285
IVR +LEK+L+++ E F + + S + Q+ + + PF+ Q+ DK
Sbjct: 19 IVRENSPRNLEKSLNVIHEWKYFDYDFGSEERRQAAIQSGEYDHTKNYPFDVDQWRDKTF 78
Query: 284 I 282
+
Sbjct: 79 V 79
>AY661557-1|AAT74557.1| 411|Apis mellifera yellow-f-like protein
protein.
Length = 411
Score = 23.8 bits (49), Expect = 1.4
Identities = 23/86 (26%), Positives = 38/86 (44%), Gaps = 11/86 (12%)
Frame = -3
Query: 506 SMNVFLYNLPEDLLRIVRLKGCDSLEK-----ALSIVLEEVNFMFQY------SSRNKML 360
S NV YN P D +R + D +E+ + I ++V S+ N L
Sbjct: 23 SWNVIEYNFPNDNIRNTLISNGDYIEENNMPNGMQIWNDKVFITIPRWKNGVPSNLNFFL 82
Query: 359 KQQNTQCTAPRPQPFNNFQFSDKNVI 282
K N + +P+ P+ N++ +D N I
Sbjct: 83 K--NDESESPKLNPYPNWEMNDINKI 106
>DQ667192-1|ABG75744.1| 489|Apis mellifera pH-sensitive chloride
channel variant 4 protein.
Length = 489
Score = 23.4 bits (48), Expect = 1.9
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +2
Query: 347 YFVVLTFYCVNYIGT 391
YF+ LTF+ ++Y GT
Sbjct: 474 YFMFLTFFFIHYKGT 488
Score = 23.0 bits (47), Expect = 2.5
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +2
Query: 347 YFVVLTFYCVNYIG 388
Y +L F CVNY+G
Sbjct: 375 YASLLEFVCVNYVG 388
>DQ667191-1|ABG75743.1| 475|Apis mellifera pH-sensitive chloride
channel variant 3 protein.
Length = 475
Score = 23.4 bits (48), Expect = 1.9
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +2
Query: 347 YFVVLTFYCVNYIGT 391
YF+ LTF+ ++Y GT
Sbjct: 460 YFMFLTFFFIHYKGT 474
Score = 23.0 bits (47), Expect = 2.5
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +2
Query: 347 YFVVLTFYCVNYIG 388
Y +L F CVNY+G
Sbjct: 344 YASLLEFVCVNYVG 357
>DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride
channel variant 1 protein.
Length = 509
Score = 23.4 bits (48), Expect = 1.9
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +2
Query: 347 YFVVLTFYCVNYIGT 391
YF+ LTF+ ++Y GT
Sbjct: 494 YFMFLTFFFIHYKGT 508
Score = 23.0 bits (47), Expect = 2.5
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +2
Query: 347 YFVVLTFYCVNYIG 388
Y +L F CVNY+G
Sbjct: 395 YASLLEFVCVNYVG 408
>DQ667189-1|ABG75741.1| 458|Apis mellifera pH-sensitive chloride
channel protein.
Length = 458
Score = 23.4 bits (48), Expect = 1.9
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +2
Query: 347 YFVVLTFYCVNYIGT 391
YF+ LTF+ ++Y GT
Sbjct: 443 YFMFLTFFFIHYKGT 457
Score = 23.0 bits (47), Expect = 2.5
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +2
Query: 347 YFVVLTFYCVNYIG 388
Y +L F CVNY+G
Sbjct: 344 YASLLEFVCVNYVG 357
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase
protein.
Length = 580
Score = 22.2 bits (45), Expect = 4.4
Identities = 7/15 (46%), Positives = 11/15 (73%)
Frame = +3
Query: 204 VDLTWLRHSVEYQIY 248
VD W ++++ YQIY
Sbjct: 22 VDANWYKNALVYQIY 36
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 170,173
Number of Sequences: 438
Number of extensions: 3945
Number of successful extensions: 18
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 19193721
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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