Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060731.seq
(640 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g32760.1 68417.m04661 VHS domain-containing protein / GAT dom... 31 0.49
At5g18440.1 68418.m02172 hypothetical protein 30 1.5
At5g64360.3 68418.m08085 DNAJ heat shock N-terminal domain-conta... 29 2.6
At5g64360.2 68418.m08084 DNAJ heat shock N-terminal domain-conta... 29 2.6
At5g64360.1 68418.m08083 DNAJ heat shock N-terminal domain-conta... 29 2.6
At3g16510.1 68416.m02107 C2 domain-containing protein contains s... 29 2.6
At2g38410.1 68415.m04718 VHS domain-containing protein / GAT dom... 29 2.6
At1g77960.1 68414.m09085 hypothetical protein 29 2.6
At5g46400.1 68418.m05711 expressed protein 29 3.4
At1g64370.1 68414.m07295 expressed protein 28 4.5
At1g53430.1 68414.m06056 leucine-rich repeat family protein / pr... 28 4.5
At5g14540.1 68418.m01704 proline-rich family protein contains pr... 28 6.0
At2g33030.1 68415.m04049 leucine-rich repeat family protein cont... 28 6.0
At5g51480.1 68418.m06385 multi-copper oxidase type I family prot... 27 7.9
At5g20710.1 68418.m02459 beta-galactosidase, putative / lactase,... 27 7.9
At1g31740.1 68414.m03894 beta-galactosidase, putative / lactase,... 27 7.9
>At4g32760.1 68417.m04661 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 838
Score = 31.5 bits (68), Expect = 0.49
Identities = 24/76 (31%), Positives = 32/76 (42%)
Frame = -2
Query: 237 IPQNVATMSNQPQQQNKFKFGIPASKQIKLPQTQQFGYRPQNQTHFGYRPPLNQNQFGYK 58
IPQN M NQP Q + +Q ++ Q + + Q Q + NQ GY
Sbjct: 703 IPQNGVYMPNQPNQALGSGYQPQQQQQQQMMMAQYYAQQQQLQQQQQQQAYGNQ-MGGYG 761
Query: 57 PNLNQPQFGYRPQLNQ 10
NQ Q G P L+Q
Sbjct: 762 YGYNQQQQGSSPYLDQ 777
>At5g18440.1 68418.m02172 hypothetical protein
Length = 451
Score = 29.9 bits (64), Expect = 1.5
Identities = 14/32 (43%), Positives = 18/32 (56%), Gaps = 2/32 (6%)
Frame = -2
Query: 117 QNQTHFGYRPPLNQNQFG-YKPN-LNQPQFGY 28
Q Q H GY+ P+N NQ G P +N P G+
Sbjct: 18 QQQQHNGYQNPMNANQLGMMNPQMMNNPMMGH 49
>At5g64360.3 68418.m08085 DNAJ heat shock N-terminal
domain-containing protein low similarity to AHM1
[Triticum aestivum] GI:6691467; contains Pfam profile
PF00226 DnaJ domain
Length = 464
Score = 29.1 bits (62), Expect = 2.6
Identities = 13/44 (29%), Positives = 24/44 (54%)
Frame = -2
Query: 162 KQIKLPQTQQFGYRPQNQTHFGYRPPLNQNQFGYKPNLNQPQFG 31
++++L Q Q G+ PQ Q+H ++ + + Y P +Q Q G
Sbjct: 142 RELQLSQLGQSGFHPQTQSHQNFQWEPSSSTAVYPPPRSQSQAG 185
>At5g64360.2 68418.m08084 DNAJ heat shock N-terminal
domain-containing protein low similarity to AHM1
[Triticum aestivum] GI:6691467; contains Pfam profile
PF00226 DnaJ domain
Length = 464
Score = 29.1 bits (62), Expect = 2.6
Identities = 13/44 (29%), Positives = 24/44 (54%)
Frame = -2
Query: 162 KQIKLPQTQQFGYRPQNQTHFGYRPPLNQNQFGYKPNLNQPQFG 31
++++L Q Q G+ PQ Q+H ++ + + Y P +Q Q G
Sbjct: 142 RELQLSQLGQSGFHPQTQSHQNFQWEPSSSTAVYPPPRSQSQAG 185
>At5g64360.1 68418.m08083 DNAJ heat shock N-terminal
domain-containing protein low similarity to AHM1
[Triticum aestivum] GI:6691467; contains Pfam profile
PF00226 DnaJ domain
Length = 422
Score = 29.1 bits (62), Expect = 2.6
Identities = 13/44 (29%), Positives = 24/44 (54%)
Frame = -2
Query: 162 KQIKLPQTQQFGYRPQNQTHFGYRPPLNQNQFGYKPNLNQPQFG 31
++++L Q Q G+ PQ Q+H ++ + + Y P +Q Q G
Sbjct: 142 RELQLSQLGQSGFHPQTQSHQNFQWEPSSSTAVYPPPRSQSQAG 185
>At3g16510.1 68416.m02107 C2 domain-containing protein contains
similarity to shock protein SRC2 [Glycine max]
gi|2055230|dbj|BAA19769 ; contains Pfam profile
PF00168:C2 domain
Length = 360
Score = 29.1 bits (62), Expect = 2.6
Identities = 17/58 (29%), Positives = 23/58 (39%), Gaps = 1/58 (1%)
Frame = -2
Query: 171 PASKQIKLPQTQQFGYRPQNQTHFGYRPPLNQNQFGY-KPNLNQPQFGYRPQLNQLVP 1
P + P F +Q+ G+ P QNQ GY P P +GY Q+ P
Sbjct: 263 PQHQSYPPPPGHSFHQTQPSQSFHGFAPSSPQNQHGYGYPPPTSPGYGYGCPTTQVPP 320
>At2g38410.1 68415.m04718 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 671
Score = 29.1 bits (62), Expect = 2.6
Identities = 24/65 (36%), Positives = 32/65 (49%), Gaps = 6/65 (9%)
Frame = -2
Query: 204 PQQQNKF-KFGIP--ASKQIKLPQTQQ-FGYRPQNQTHFGY-RPPLNQNQFGYKP-NLNQ 43
PQ Q +F + P +Q + PQ QQ + Q+Q GY +P +Q Q GY Q
Sbjct: 469 PQPQPRFDSYVAPWAQQQQPQQPQAQQGYSQHQQHQQQQGYSQPQHSQQQQGYSQLQQPQ 528
Query: 42 PQFGY 28
PQ GY
Sbjct: 529 PQQGY 533
>At1g77960.1 68414.m09085 hypothetical protein
Length = 211
Score = 29.1 bits (62), Expect = 2.6
Identities = 24/87 (27%), Positives = 35/87 (40%), Gaps = 8/87 (9%)
Frame = -2
Query: 237 IPQNVATMSNQPQQQNKFKFGI----PASKQIKLPQTQQFGYRPQNQT---HFGYRPPLN 79
+PQN P QQ FG+ P Q + R N H G + +N
Sbjct: 43 LPQNYMPYQT-PHQQVSSSFGMYPQQPRPPVFVRRQNEPPNIRMVNNNALQHGGMQTSVN 101
Query: 78 QNQFGYK-PNLNQPQFGYRPQLNQLVP 1
Q+ ++ PN+N Q RP N ++P
Sbjct: 102 QSPLRFRLPNINNRQAPVRPNANAIIP 128
>At5g46400.1 68418.m05711 expressed protein
Length = 1036
Score = 28.7 bits (61), Expect = 3.4
Identities = 24/79 (30%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = -2
Query: 237 IPQN-VATMSNQPQQQNKFKFGIPASKQIKLPQTQQFGYRPQNQTHFGYRPPLNQNQ-FG 64
+ QN V +S Q Q Q K ++ P +Q++ TQQ PQNQ ++ Q + F
Sbjct: 914 LDQNLVQLLSKQYQSQAKTQYLQP--QQVEQVNTQQQSQEPQNQQQIQFQQQQQQQEWFQ 971
Query: 63 YKPNLNQPQFGYRPQLNQL 7
+ Q Q+ Q QL
Sbjct: 972 QQQQWQQQQYLLYIQQQQL 990
>At1g64370.1 68414.m07295 expressed protein
Length = 178
Score = 28.3 bits (60), Expect = 4.5
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 5/40 (12%)
Frame = -2
Query: 117 QNQTHFGY-----RPPLNQNQFGYKPNLNQPQFGYRPQLN 13
Q+Q+ GY R NQN+ GY + ++P +G P LN
Sbjct: 35 QSQSRGGYGQIHNREGYNQNREGYSQSQSRPVYGLSPTLN 74
>At1g53430.1 68414.m06056 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 1030
Score = 28.3 bits (60), Expect = 4.5
Identities = 23/86 (26%), Positives = 35/86 (40%), Gaps = 4/86 (4%)
Frame = -3
Query: 482 LPEDLLRIVRLKGCDSLEKALSIVLEEVNFMFQYSSRNKMLKQQNTQCTAPRPQPF---- 315
+PE + + LK D L+ V+ + F+ + N T P PQ
Sbjct: 290 IPEYIGSMSELKTLDLSSNMLTGVIPDT---FRNLDAFNFMFLNNNSLTGPVPQFIINSK 346
Query: 314 NNFQFSDKNVIKPHFISNTQLSINLV 237
N SD N +P +S QL +NL+
Sbjct: 347 ENLDLSDNNFTQPPTLSCNQLDVNLI 372
>At5g14540.1 68418.m01704 proline-rich family protein contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 547
Score = 27.9 bits (59), Expect = 6.0
Identities = 21/61 (34%), Positives = 29/61 (47%)
Frame = -2
Query: 204 PQQQNKFKFGIPASKQIKLPQTQQFGYRPQNQTHFGYRPPLNQNQFGYKPNLNQPQFGYR 25
PQQ+ F P S Q + P T Q Y+P T ++PP Y+P QPQ+ +
Sbjct: 292 PQQEPYF----PPSGQSQPPPTIQPPYQPPPPTQSLHQPP-------YQPPPQQPQYPQQ 340
Query: 24 P 22
P
Sbjct: 341 P 341
>At2g33030.1 68415.m04049 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611
Length = 218
Score = 27.9 bits (59), Expect = 6.0
Identities = 20/72 (27%), Positives = 32/72 (44%), Gaps = 7/72 (9%)
Frame = -3
Query: 509 LSMNVFLYNLPEDLLRIVRLKGCDSLEKALSIV----LEEVNFMFQYSSRNKMLK---QQ 351
LS N F+ N+P + ++ L+ D LS L+ ++F+ + + LK Q
Sbjct: 66 LSNNAFIGNIPMSMANLIELESLDMSRNGLSGTIPQGLKTLSFLGYINVSHNQLKGEIPQ 125
Query: 350 NTQCTAPRPQPF 315
TQ T P F
Sbjct: 126 GTQITGPPKSSF 137
>At5g51480.1 68418.m06385 multi-copper oxidase type I family protein
contains Pfam profile: PF00394 Multicopper oxidase;
similar to pollen-specific protein
Length = 592
Score = 27.5 bits (58), Expect = 7.9
Identities = 12/52 (23%), Positives = 26/52 (50%)
Frame = -3
Query: 422 LSIVLEEVNFMFQYSSRNKMLKQQNTQCTAPRPQPFNNFQFSDKNVIKPHFI 267
L + +VN + ++ + +KQ NT + RP P +F + N+ + + +
Sbjct: 321 LPVSATDVNHPWSAMNQPRAIKQ-NTSASGARPNPQGSFHYGQINITRTYIL 371
>At5g20710.1 68418.m02459 beta-galactosidase, putative / lactase,
putative strong similarity to beta-galactosidase
precursor (EC 3.2.1.23) (Lactase) SP:P49676 from
[Brassica oleracea]
Length = 769
Score = 27.5 bits (58), Expect = 7.9
Identities = 15/39 (38%), Positives = 24/39 (61%)
Frame = -2
Query: 120 PQNQTHFGYRPPLNQNQFGYKPNLNQPQFGYRPQLNQLV 4
P T + Y PL++ FG NLNQP++G+ QL+ ++
Sbjct: 250 PYITTSYDYHAPLDE--FG---NLNQPKWGHLKQLHTVL 283
>At1g31740.1 68414.m03894 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase precursor
[Brassica oleracea] SWISS-PROT:P49676
Length = 786
Score = 27.5 bits (58), Expect = 7.9
Identities = 15/39 (38%), Positives = 24/39 (61%)
Frame = -2
Query: 120 PQNQTHFGYRPPLNQNQFGYKPNLNQPQFGYRPQLNQLV 4
P T + Y PL++ FG NLNQP++G+ QL+ ++
Sbjct: 324 PYITTTYDYDAPLDE--FG---NLNQPKYGHLKQLHDVL 357
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,392,873
Number of Sequences: 28952
Number of extensions: 235931
Number of successful extensions: 634
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 603
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 632
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1314848736
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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