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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060730.seq
         (685 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_19540| Best HMM Match : Pro_isomerase (HMM E-Value=1.5e-23)        101   7e-22
SB_34931| Best HMM Match : C4dic_mal_tran (HMM E-Value=0.46)           31   1.1  
SB_36994| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.5  
SB_52865| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   2.0  
SB_23757| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.6  

>SB_19540| Best HMM Match : Pro_isomerase (HMM E-Value=1.5e-23)
          Length = 741

 Score =  101 bits (241), Expect = 7e-22
 Identities = 43/75 (57%), Positives = 57/75 (76%)
 Frame = +2

Query: 281 RGVLKRNFKHLAVDIRMVNPRLLKVEKWFGSKKELAAVRTVCSHVENMIKGVTKGFQYKM 460
           RG LKRNF+HL +++  V    ++V+ WF S+KELA V+T+ +H+ENMIKGV  G++YKM
Sbjct: 587 RGTLKRNFRHLRLELTKVGKDKVRVDVWFASRKELACVKTIITHIENMIKGVIYGYRYKM 646

Query: 461 RAVYAHFPINCVTTE 505
           RAVYAHFPIN    E
Sbjct: 647 RAVYAHFPINIAIQE 661



 Score = 37.1 bits (82), Expect = 0.013
 Identities = 13/19 (68%), Positives = 19/19 (100%)
 Frame = +1

Query: 511 SIIEIRNFLGEKYIRRVKM 567
           +++E+RNFLGEKY+RRV+M
Sbjct: 664 TLVEVRNFLGEKYVRRVRM 682


>SB_34931| Best HMM Match : C4dic_mal_tran (HMM E-Value=0.46)
          Length = 572

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 21/66 (31%), Positives = 34/66 (51%)
 Frame = -3

Query: 245 NATSNFTREASNLFITLLNEISSNSTEPVDVFAREADSTAKNTTGRTKLRSIVLNEHNY* 66
           N T+N TREA++  I  L  +  N+T    + A  A STA  T   + + ++   + +  
Sbjct: 471 NVTTNMTREANSTMILPLTSVPYNTTL---IPAPNATSTAATTMSPSYVANMTTLDSSVS 527

Query: 65  LERSIL 48
           L RS+L
Sbjct: 528 LTRSLL 533



 Score = 29.1 bits (62), Expect = 3.5
 Identities = 21/66 (31%), Positives = 33/66 (50%)
 Frame = -3

Query: 245 NATSNFTREASNLFITLLNEISSNSTEPVDVFAREADSTAKNTTGRTKLRSIVLNEHNY* 66
           N T+N TREA++  I  L  +  N+T    + A  A STA  T   + + ++   + +  
Sbjct: 69  NVTTNMTREANSTMILPLTSVPYNTTL---IPAPNATSTAATTMSPSYVANMTTLDSSVS 125

Query: 65  LERSIL 48
           L RS L
Sbjct: 126 LTRSSL 131


>SB_36994| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 618

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 23/69 (33%), Positives = 35/69 (50%), Gaps = 2/69 (2%)
 Frame = +1

Query: 73  LCSLRTMLRNFVLPVVFLAVLSASLANTSTGSVELDEISFNKVI--NKFEASLVKFDVAF 246
           LC+ +T+LR+  L +  LAV+ A+        ++ +E S       N    S +  D +F
Sbjct: 421 LCT-KTILRSVSLVIAVLAVVVATPTYFFAKEIKENESSLGHFTGRNFTRGSFLASDFSF 479

Query: 247 PYGINTMLL 273
            YGI TMLL
Sbjct: 480 GYGIFTMLL 488


>SB_52865| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 862

 Score = 29.9 bits (64), Expect = 2.0
 Identities = 16/39 (41%), Positives = 25/39 (64%)
 Frame = -1

Query: 532 SYVSQ*LNTLSGDTVNGEVSIHSTHLVLEAFSYSFNHVL 416
           SY+S  L +LSG+ + G+V  HS  ++L  +S   N+VL
Sbjct: 94  SYISPLLASLSGEQLPGKVIAHSGQMLLNLYS-DTNYVL 131


>SB_23757| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2834

 Score = 28.7 bits (61), Expect = 4.6
 Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 5/93 (5%)
 Frame = +2

Query: 374  KKELAAVRTVCSHVENMIKGVTKGFQY-KMRAVYAHFPI--NCVTTEGIQLLRYV-TSWG 541
            K+ LAA + V +   N+ KGVT+G    K R V    PI    V T+G  + + +  + G
Sbjct: 948  KRALAAAKVVKTERVNVAKGVTQGMPVTKGRVVTQGMPITPGRVVTQGKVVTQGMPVTPG 1007

Query: 542  RNTSEG*RWAPGVTVVNS-PKQKXELIIEGQLL 637
            R  ++G    PG  V    P  +  ++ +G ++
Sbjct: 1008 RVVTQGIPVTPGRIVTQGIPVTQGRVVTQGTVV 1040


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,140,702
Number of Sequences: 59808
Number of extensions: 404216
Number of successful extensions: 927
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 844
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 927
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1769412099
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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