BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060718.seq (472 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 98 5e-22 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 98 5e-22 SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 3.3 SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 26 3.3 SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizos... 25 4.4 SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces... 25 7.7 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 98.3 bits (234), Expect = 5e-22 Identities = 43/48 (89%), Positives = 44/48 (91%) Frame = +1 Query: 256 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGXRMFAPTK 399 GHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR RMFAPTK Sbjct: 59 GHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTK 106 Score = 66.9 bits (156), Expect = 1e-12 Identities = 31/58 (53%), Positives = 41/58 (70%) Frame = +2 Query: 80 LSVARPLVSVYSEKSXTVQGAAKPLPFVFKAXIRPDLVNDVHVSMSKNSRQPYCVSKE 253 ++ ARP VS+Y+ K +V LPFVFKA IRPDLV VH +++KN RQPY VS++ Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 98.3 bits (234), Expect = 5e-22 Identities = 43/48 (89%), Positives = 44/48 (91%) Frame = +1 Query: 256 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGXRMFAPTK 399 GHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR RMFAPTK Sbjct: 59 GHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTK 106 Score = 67.7 bits (158), Expect = 8e-13 Identities = 32/58 (55%), Positives = 41/58 (70%) Frame = +2 Query: 80 LSVARPLVSVYSEKSXTVQGAAKPLPFVFKAXIRPDLVNDVHVSMSKNSRQPYCVSKE 253 ++ ARP VS+YS K +V LPFVFKA IRPDLV VH +++KN RQPY VS++ Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57 >SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 25.8 bits (54), Expect = 3.3 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = -2 Query: 141 APCTVXLFSEYTDTKGRATDRLISLL 64 A C V ++SEYT G ++ L++L+ Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515 >SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltransferase Pis1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 25.8 bits (54), Expect = 3.3 Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%) Frame = -3 Query: 98 KVG-LPIDSFRYFSEAIPLKY-TLIAVI 21 K+G LP+ SF Y + +PL Y TL+AV+ Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213 >SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 532 Score = 25.4 bits (53), Expect = 4.4 Identities = 12/37 (32%), Positives = 18/37 (48%) Frame = +1 Query: 79 SIGSPTFSVGVFREEXDGAGCSQAPPVRVQGXHPSGP 189 SI SP+ SVG + D + S + P+ + G P Sbjct: 312 SITSPSSSVGQIPQPTDHSALSPSKPMSISGTESPNP 348 >SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1441 Score = 24.6 bits (51), Expect = 7.7 Identities = 9/29 (31%), Positives = 18/29 (62%) Frame = +2 Query: 218 KNSRQPYCVSKEVVTKPVLNHGVLDVLSP 304 ++ +PY ++ + P+ N G LD++SP Sbjct: 1054 EDGNEPYAITDDNDYLPMANTGSLDLVSP 1082 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,630,150 Number of Sequences: 5004 Number of extensions: 29234 Number of successful extensions: 61 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 57 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 60 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 180421690 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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