BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060715.seq (641 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g56560.1 68418.m07058 F-box family protein contains F-box dom... 30 1.1 At4g01560.1 68417.m00202 brix domain-containing protein contains... 29 2.0 At2g22900.1 68415.m02718 galactosyl transferase GMA12/MNN10 fami... 29 2.6 At5g20200.1 68418.m02406 nucleoporin-related contains weak simil... 29 3.5 At4g17483.1 68417.m02615 palmitoyl protein thioesterase family p... 29 3.5 At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor... 28 4.6 At5g50060.1 68418.m06199 invertase/pectin methylesterase inhibit... 28 6.1 At4g24240.1 68417.m03479 WRKY family transcription factor contai... 27 8.0 At1g75340.1 68414.m08751 zinc finger (CCCH-type) family protein ... 27 8.0 >At5g56560.1 68418.m07058 F-box family protein contains F-box domain Pfam:PF00646 Length = 607 Score = 30.3 bits (65), Expect = 1.1 Identities = 16/56 (28%), Positives = 30/56 (53%) Frame = +3 Query: 93 VCVIITIKMVAGGKQMNVRVTTMDAELEFAIQQTTTGKQLFDQVVKTIGLREVWSS 260 +C ++ +V G K +NV++ MD ++ +TG+ + + KT+GL SS Sbjct: 175 ICPVLERLVVDGTKGVNVKIPNMDVPNLRSLSFRSTGELRIELLRKTLGLPTCLSS 230 >At4g01560.1 68417.m00202 brix domain-containing protein contains Pfam domain, PF04427: Brix domain Length = 343 Score = 29.5 bits (63), Expect = 2.0 Identities = 22/67 (32%), Positives = 31/67 (46%) Frame = +2 Query: 302 KLYKKVMQQDVKKENPLQFKFRAKFYPEDVADELIQEITLKLFYLQVKNAILSDEIYCPP 481 K K +Q +K+ Q K RA+ E A EL +E K+ ++N SDE C P Sbjct: 44 KRSKVYAKQKHEKKLEKQKKIRARDAAEKRALELGEEPPQKMIPKTIENTRESDETVCRP 103 Query: 482 ETSVLLA 502 + L A Sbjct: 104 DDEELFA 110 >At2g22900.1 68415.m02718 galactosyl transferase GMA12/MNN10 family protein very low similarity to alpha-1,2-galactosyltransferase, Schizosaccharomyces pombe [SP|Q09174] Length = 449 Score = 29.1 bits (62), Expect = 2.6 Identities = 13/26 (50%), Positives = 16/26 (61%), Gaps = 1/26 (3%) Frame = +1 Query: 502 FVPVQARHGEHNPALHG-PGFLANDR 576 F P R+ EHN +HG PG + NDR Sbjct: 211 FTPPWRRYKEHNLVVHGWPGVIYNDR 236 >At5g20200.1 68418.m02406 nucleoporin-related contains weak similarity to Nucleoporin NUP1 (Nuclear pore protein NUP1) (Swiss-Prot:P20676) [Saccharomyces cerevisiae] Length = 762 Score = 28.7 bits (61), Expect = 3.5 Identities = 12/29 (41%), Positives = 20/29 (68%) Frame = +3 Query: 210 LFDQVVKTIGLREVWSSVFSTPTPRATSL 296 LFD+ + IG ++ S +++TPTP A S+ Sbjct: 213 LFDKAKEPIGGKDANSEIWATPTPLAKSI 241 >At4g17483.1 68417.m02615 palmitoyl protein thioesterase family protein Length = 300 Score = 28.7 bits (61), Expect = 3.5 Identities = 16/64 (25%), Positives = 28/64 (43%) Frame = +1 Query: 421 ETLLSTGEKRDFIR*NILPSGDLGVAGFVPVQARHGEHNPALHGPGFLANDRLLPQRVTD 600 E LL DF++ +I PSG + + G + H ++ P L+ + R T+ Sbjct: 152 EALLKLEVYTDFVQDHIAPSGYIKIPGEISKYLEHSKYLPKLNNERPDERNSTFKDRFTN 211 Query: 601 QHKM 612 H + Sbjct: 212 LHNL 215 >At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2 (CPK2) identical to calcium-dependent protein kinase isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 646 Score = 28.3 bits (60), Expect = 4.6 Identities = 18/70 (25%), Positives = 35/70 (50%) Frame = +2 Query: 320 MQQDVKKENPLQFKFRAKFYPEDVADELIQEITLKLFYLQVKNAILSDEIYCPPETSVLL 499 +Q + KKE L + + K PE+ E+ E + + +Q ++A + PET+ Sbjct: 83 IQPEEKKEKVLAEETKQKVVPEESKQEVPPEESKREVVVQPESAKPETKSESKPETTKPE 142 Query: 500 ASYRSKPDTE 529 + +KP+T+ Sbjct: 143 TTSETKPETK 152 >At5g50060.1 68418.m06199 invertase/pectin methylesterase inhibitor family protein low similarity to pollen-specific protein Bnm1 [Brassica napus] GI:1857671; contains Pfam profile PF04043: Plant invertase/pectin methylesterase inhibitor Length = 166 Score = 27.9 bits (59), Expect = 6.1 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +3 Query: 78 DLIRCVCVIITIKMVAGGKQMNVRVTTMDAELE 176 D+ RC + + +K V GG + N T MD L+ Sbjct: 117 DIFRCTDITMYLKDVVGGNRDNTSKTFMDMTLQ 149 >At4g24240.1 68417.m03479 WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA -binding domain Length = 353 Score = 27.5 bits (58), Expect = 8.0 Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 2/39 (5%) Frame = +3 Query: 294 LGSSCIRR*CNKTSRKRIHCSS--NSEPNSILRMSPMSS 404 L S+ ++R CN + R HCS S ++R+ +SS Sbjct: 236 LSSASLKRRCNSSPSSRCHCSKKRKSRVKRVIRVPAVSS 274 >At1g75340.1 68414.m08751 zinc finger (CCCH-type) family protein weak similarity to Nucleoporin NUP42 (Nuclear pore protein NUP42) (Swiss-Prot:P49686) [Saccharomyces cerevisiae]; contains Pfam profile PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) Length = 435 Score = 27.5 bits (58), Expect = 8.0 Identities = 17/52 (32%), Positives = 26/52 (50%), Gaps = 4/52 (7%) Frame = +2 Query: 311 KKVMQQDVKKENPLQFKFRA----KFYPEDVADELIQEITLKLFYLQVKNAI 454 K+VMQ D K E P+ +K K++P DV ++ E + Y + K I Sbjct: 112 KRVMQDDFKNERPM-WKLTCYGHWKYFPCDVTGDISYEELRAVAYEEAKRGI 162 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,492,532 Number of Sequences: 28952 Number of extensions: 305439 Number of successful extensions: 906 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 882 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 906 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1324661040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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