BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060712.seq (639 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_7742| Best HMM Match : HEAT (HMM E-Value=9e-23) 89 3e-18 SB_29863| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.4 SB_8160| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7 >SB_7742| Best HMM Match : HEAT (HMM E-Value=9e-23) Length = 940 Score = 89.0 bits (211), Expect = 3e-18 Identities = 50/133 (37%), Positives = 77/133 (57%) Frame = +3 Query: 237 KKGL*CVSELARNHIDDDGNNQWPEFLQFMFTCASAQDPNIKEAGIRMFTSVPGVFGNRQ 416 KK VSEL+++ +DDDG N W E L+F+F C ++ +KE+ + +F S PGVFGN+Q Sbjct: 76 KKICDAVSELSKSFLDDDGYNHWQELLKFLFECCNSPRAELKESALNIFCSFPGVFGNQQ 135 Query: 417 TENLDVIKGMLISALQPNESMALRTQGS*S*GAFILLHDKEPIIQKHFSDVLLPLMQVIV 596 L+VIK ML + S A+R + + AFI E Q+ F +++ ++Q + Sbjct: 136 DHYLNVIKQMLWQCINDQTSQAVRFVAARASCAFITDQVGE-AKQRQFVELVPGIIQTVR 194 Query: 597 LSIEAADDDSALK 635 S A+ DD+ LK Sbjct: 195 ESALASGDDAVLK 207 Score = 37.5 bits (83), Expect = 0.009 Identities = 20/55 (36%), Positives = 31/55 (56%) Frame = +1 Query: 88 DVRQTAAVLLRRLFSAXXXXXXXXXXXXQQTVLREQLLLTLQMDLSQYLRRKVCD 252 +VRQ AAVLLRR+F+A Q +++E LL + + +R+K+CD Sbjct: 27 EVRQMAAVLLRRIFTA-TVDFLKKIDENTQNLMKESLLKGIHEEQDSNVRKKICD 80 >SB_29863| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 590 Score = 27.9 bits (59), Expect = 7.4 Identities = 21/56 (37%), Positives = 28/56 (50%) Frame = +1 Query: 250 DVSLNWLGIILMTMATTSGLSSYSSCLPVPVRKIQTSKKLVLECLRLYQVYLEIVR 417 D+S++W GI+L+T LS S C VRK VLE L L Q+ + R Sbjct: 302 DLSISWHGILLVTGDVNVDLSRPSECY---VRKYMD----VLESLNLTQLVTKATR 350 >SB_8160| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 516 Score = 27.5 bits (58), Expect = 9.7 Identities = 18/55 (32%), Positives = 27/55 (49%), Gaps = 5/55 (9%) Frame = -2 Query: 362 FDVWILRTGTGKH-EL*----ELRPLVVAIVINMIPSQFRDTSQTFLRKYWERSI 213 F W G+G+H EL L + + +N IPSQF + + KYW++ I Sbjct: 119 FSQWFFIRGSGQHSELDYPCVSLSSVDTILFVN-IPSQFYARIKELILKYWDKGI 172 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,791,851 Number of Sequences: 59808 Number of extensions: 397116 Number of successful extensions: 964 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 898 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 963 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1608851125 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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