BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060705.seq
(439 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g42440.1 68414.m04894 expressed protein contains Pfam domain,... 30 0.79
At3g49500.1 68416.m05410 RNA-dependent RNA polymerase (SDE1) ide... 28 3.2
At3g48770.1 68416.m05326 hypothetical protein 27 5.5
At3g11820.2 68416.m01448 syntaxin 121 (SYP121) / syntaxin-relate... 27 5.5
At3g11820.1 68416.m01449 syntaxin 121 (SYP121) / syntaxin-relate... 27 5.5
At5g57655.2 68418.m07204 xylose isomerase family protein contain... 27 7.3
At5g57655.1 68418.m07203 xylose isomerase family protein contain... 27 7.3
At3g60080.1 68416.m06709 zinc finger (C3HC4-type RING finger) fa... 27 7.3
At3g57880.1 68416.m06452 C2 domain-containing protein contains I... 26 9.7
>At1g42440.1 68414.m04894 expressed protein contains Pfam domain,
PF04950: Protein of unknown function (DUF663)
Length = 793
Score = 29.9 bits (64), Expect = 0.79
Identities = 17/61 (27%), Positives = 26/61 (42%)
Frame = +3
Query: 204 SGYWNNSRGNGGSKFGGATNSAIIRLDLATVAVXLEDLEDFVGKKNSLEVRTCVAQIGXL 383
S W + N FG S + L + V + DL V KKN ++ + C +Q+
Sbjct: 155 SAPWEENSSNFIDSFGSQCLSVFRSIGLPSTTVLIRDLPSDVKKKNEMK-KMCASQLASE 213
Query: 384 F 386
F
Sbjct: 214 F 214
>At3g49500.1 68416.m05410 RNA-dependent RNA polymerase (SDE1)
identical to RNA-dependent RNA polymerase [Arabidopsis
thaliana] gi|8248473|gb|AAF74208
Length = 1196
Score = 27.9 bits (59), Expect = 3.2
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +3
Query: 306 LEDLEDFVGKKNSLEVRTCVAQIGXLFHSXLSTKTFMXH 422
+ D++ ++GK V C A++G F S +T M H
Sbjct: 530 VSDIKTWMGKFKDKNVAKCAARMGLCFSSTYATVDVMPH 568
>At3g48770.1 68416.m05326 hypothetical protein
Length = 1899
Score = 27.1 bits (57), Expect = 5.5
Identities = 23/87 (26%), Positives = 37/87 (42%)
Frame = +3
Query: 114 SCRIRHEETLRXKESIVXINIFNXIRKLXMSGYWNNSRGNGGSKFGGATNSAIIRLDLAT 293
S ++ E LR S+V I++ + + S + G K A +IR +
Sbjct: 1756 SLKVETAERLRIIHSLVDIDVKETSETITTE--YTLSLPSKGEKLI-AKAKRMIRWEREK 1812
Query: 294 VAVXLEDLEDFVGKKNSLEVRTCVAQI 374
V E +E GK+ LE TC A++
Sbjct: 1813 GVVYAEKMEKTCGKRKLLEYATCFAEV 1839
>At3g11820.2 68416.m01448 syntaxin 121 (SYP121) / syntaxin-related
protein (SYR1) contains Pfam profiles: PF00804 syntaxin
and PF05739: SNARE domain; identical to cDNA
syntaxin-related protein At-SYR1 (At-Syr1) GI:4206788,
SP|Q9ZSD4 Syntaxin 121 (AtSYP121) (Syntaxin-related
protein At-Syr1) {Arabidopsis thaliana}
Length = 315
Score = 27.1 bits (57), Expect = 5.5
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +3
Query: 156 SIVXINIFNXIRKLXMSGYWNNSRGNGGSKFGGAT 260
+I+ + I + L + WNNS G GG GG T
Sbjct: 259 AIIILIIIITVVVLAVLKPWNNSSGGGGGGGGGGT 293
>At3g11820.1 68416.m01449 syntaxin 121 (SYP121) / syntaxin-related
protein (SYR1) contains Pfam profiles: PF00804 syntaxin
and PF05739: SNARE domain; identical to cDNA
syntaxin-related protein At-SYR1 (At-Syr1) GI:4206788,
SP|Q9ZSD4 Syntaxin 121 (AtSYP121) (Syntaxin-related
protein At-Syr1) {Arabidopsis thaliana}
Length = 346
Score = 27.1 bits (57), Expect = 5.5
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +3
Query: 156 SIVXINIFNXIRKLXMSGYWNNSRGNGGSKFGGAT 260
+I+ + I + L + WNNS G GG GG T
Sbjct: 290 AIIILIIIITVVVLAVLKPWNNSSGGGGGGGGGGT 324
>At5g57655.2 68418.m07204 xylose isomerase family protein contains
similarity to Xylose isomerase (EC 5.3.1.5)
(Swiss-Prot:P22842) [Thermoanaerobacter ethanolicus]
Length = 477
Score = 26.6 bits (56), Expect = 7.3
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = +3
Query: 210 YWNNSRGNGGSKFGGAT 260
+W+ RG GG FG AT
Sbjct: 89 FWHTFRGTGGDPFGAAT 105
>At5g57655.1 68418.m07203 xylose isomerase family protein contains
similarity to Xylose isomerase (EC 5.3.1.5)
(Swiss-Prot:P22842) [Thermoanaerobacter ethanolicus]
Length = 287
Score = 26.6 bits (56), Expect = 7.3
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = +3
Query: 210 YWNNSRGNGGSKFGGAT 260
+W+ RG GG FG AT
Sbjct: 89 FWHTFRGTGGDPFGAAT 105
>At3g60080.1 68416.m06709 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 306
Score = 26.6 bits (56), Expect = 7.3
Identities = 16/35 (45%), Positives = 24/35 (68%), Gaps = 2/35 (5%)
Frame = +3
Query: 249 GGATNSAIIRL-DLATVAVXLEDLE-DFVGKKNSL 347
GG+ IRL DLAT+A +D+E D++G +N+L
Sbjct: 222 GGSEAEMRIRLSDLATIAADGDDVEDDWLGIRNAL 256
>At3g57880.1 68416.m06452 C2 domain-containing protein contains
INTERPRO:IPR000008 C2 domain
Length = 773
Score = 26.2 bits (55), Expect = 9.7
Identities = 13/32 (40%), Positives = 21/32 (65%), Gaps = 2/32 (6%)
Frame = +3
Query: 207 GYWNNSRGNGGSKFGGATNSAI--IRLDLATV 296
G ++N +GG K GGA +S I +R+ L+T+
Sbjct: 430 GVFDNCHLHGGEKIGGAKDSRIGKVRIRLSTL 461
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,758,867
Number of Sequences: 28952
Number of extensions: 83400
Number of successful extensions: 203
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 692941200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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