SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060693.seq
         (642 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g55150.1 68414.m06298 DEAD box RNA helicase, putative (RH20) ...   103   1e-22
At5g63120.2 68418.m07924 ethylene-responsive DEAD box RNA helica...    91   8e-19
At5g63120.1 68418.m07925 ethylene-responsive DEAD box RNA helica...    91   8e-19
At2g47330.1 68415.m05908 DEAD/DEAH box helicase, putative simila...    74   9e-14
At3g01540.3 68416.m00084 DEAD box RNA helicase (DRH1) identical ...    71   9e-13
At3g01540.2 68416.m00083 DEAD box RNA helicase (DRH1) identical ...    71   9e-13
At3g01540.1 68416.m00082 DEAD box RNA helicase (DRH1) identical ...    71   9e-13
At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative simila...    65   4e-11
At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar...    64   6e-11
At3g06480.1 68416.m00750 DEAD box RNA helicase, putative similar...    63   2e-10
At4g33370.1 68417.m04744 DEAD-box protein abstrakt, putative RNA...    54   6e-08
At5g51280.1 68418.m06357 DEAD-box protein abstrakt, putative           50   2e-06
At3g09720.1 68416.m01151 DEAD/DEAH box helicase, putative simila...    44   7e-05
At4g16630.1 68417.m02514 DEAD/DEAH box helicase, putative (RH28)...    43   2e-04
At2g33730.1 68415.m04134 DEAD box RNA helicase, putative similar...    39   0.002
At3g02065.2 68416.m00170 DEAD/DEAH box helicase family protein c...    38   0.004
At2g42520.1 68415.m05262 DEAD box RNA helicase, putative similar...    38   0.006
At1g31970.1 68414.m03931 DEAD/DEAH box helicase, putative simila...    38   0.006
At5g14610.1 68418.m01713 DEAD box RNA helicase, putative similar...    36   0.023
At1g77050.1 68414.m08971 DEAD/DEAH box helicase, putative simila...    36   0.023
At3g58570.1 68416.m06528 DEAD box RNA helicase, putative similar...    35   0.053
At3g58510.2 68416.m06522 DEAD box RNA helicase, putative (RH11) ...    33   0.12 
At3g58510.1 68416.m06521 DEAD box RNA helicase, putative (RH11) ...    33   0.12 
At1g51380.1 68414.m05780 eukaryotic translation initiation facto...    32   0.28 
At4g00660.2 68417.m00091 DEAD/DEAH box helicase, putative simila...    32   0.37 
At4g00660.1 68417.m00090 DEAD/DEAH box helicase, putative simila...    32   0.37 
At4g34910.1 68417.m04950 DEAD/DEAH box helicase, putative (RH16)...    31   0.65 
At5g60990.1 68418.m07651 DEAD/DEAH box helicase, putative (RH10)...    30   1.1  
At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contai...    30   1.5  
At3g61240.2 68416.m06854 DEAD/DEAH box helicase, putative (RH12)...    30   1.5  
At3g61240.1 68416.m06853 DEAD/DEAH box helicase, putative (RH12)...    30   1.5  
At2g45810.1 68415.m05697 DEAD/DEAH box helicase, putative              30   1.5  
At2g40700.1 68415.m05021 DEAD/DEAH box helicase, putative (RH17)...    29   2.0  
At5g43990.2 68418.m05382 SET domain-containing protein identical...    29   3.5  
At5g43990.1 68418.m05383 SET domain-containing protein identical...    29   3.5  
At3g49500.1 68416.m05410 RNA-dependent RNA polymerase (SDE1) ide...    28   4.6  
At2g33420.1 68415.m04096 expressed protein                             28   4.6  
At2g02440.1 68415.m00183 hypothetical protein                          28   4.6  
At1g16280.1 68414.m01949 DEAD/DEAH box helicase, putative simila...    28   4.6  
At1g12770.1 68414.m01482 DEAD/DEAH box helicase family protein /...    28   6.1  
At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes...    28   6.1  
At2g36300.1 68415.m04455 integral membrane Yip1 family protein c...    27   8.0  
At2g25460.1 68415.m03049 expressed protein                             27   8.0  
At2g01830.3 68415.m00115 histidine kinase (AHK4) (WOL) identical...    27   8.0  
At2g01830.2 68415.m00116 histidine kinase (AHK4) (WOL) identical...    27   8.0  
At2g01830.1 68415.m00114 histidine kinase (AHK4) (WOL) identical...    27   8.0  

>At1g55150.1 68414.m06298 DEAD box RNA helicase, putative (RH20)
           similar to ethylene-responsive RNA helicase GI:5669638
           from [Lycopersicon esculentum]; contains Pfam profiles
           PF00270: DEAD/DEAH box helicase, PF00271: Helicase
           conserved C-terminal domain
          Length = 501

 Score =  103 bits (247), Expect = 1e-22
 Identities = 48/91 (52%), Positives = 59/91 (64%), Gaps = 1/91 (1%)
 Frame = +3

Query: 243 SPR-LDSVSLQPFNKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANF 419
           SPR LD   L PF KNFY   P V   +  EVEEYR   E+TV G ++  P++ F +  F
Sbjct: 47  SPRKLDLDGLTPFEKNFYVESPAVAAMTDTEVEEYRKLREITVEGKDIPKPVKSFRDVGF 106

Query: 420 PDYVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
           PDYV + VK  G+ EPTPIQ+QGWP+AM GR
Sbjct: 107 PDYVLEEVKKAGFTEPTPIQSQGWPMAMKGR 137



 Score = 44.4 bits (100), Expect = 7e-05
 Identities = 20/44 (45%), Positives = 27/44 (61%)
 Frame = +2

Query: 509 KNLVGVLKRVPAKRWPTSXPAIVHINNQPPIRRXDGPIALVLAP 640
           ++L+G+ +    K      PAIVH+N QP +   DGPI LVLAP
Sbjct: 137 RDLIGIAETGSGKTLSYLLPAIVHVNAQPMLAHGDGPIVLVLAP 180


>At5g63120.2 68418.m07924 ethylene-responsive DEAD box RNA helicase,
           putative (RH30) strong similarity to ethylene-responsive
           RNA helicase [Lycopersicon esculentum] GI:5669638;
           contains Pfam profiles PF00270: DEAD/DEAH box helicase,
           PF00271: Helicase conserved C-terminal domain
          Length = 591

 Score = 90.6 bits (215), Expect = 8e-19
 Identities = 38/89 (42%), Positives = 58/89 (65%)
 Frame = +3

Query: 246 PRLDSVSLQPFNKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPD 425
           P+ +  +L  F KNFY   PTV   +  +V  YR   +++V G +V  P++ F++ANFPD
Sbjct: 115 PKQNFGNLVHFEKNFYVESPTVQAMTEQDVAMYRTERDISVEGRDVPKPMKMFQDANFPD 174

Query: 426 YVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
            + + +  +G+ EPTPIQAQGWP+A+ GR
Sbjct: 175 NILEAIAKLGFTEPTPIQAQGWPMALKGR 203



 Score = 41.1 bits (92), Expect = 6e-04
 Identities = 17/44 (38%), Positives = 28/44 (63%)
 Frame = +2

Query: 509 KNLVGVLKRVPAKRWPTSXPAIVHINNQPPIRRXDGPIALVLAP 640
           ++L+G+ +    K      PA+VH++ QP + + DGPI L+LAP
Sbjct: 203 RDLIGIAETGSGKTLAYLLPALVHVSAQPRLGQDDGPIVLILAP 246


>At5g63120.1 68418.m07925 ethylene-responsive DEAD box RNA helicase,
           putative (RH30) strong similarity to ethylene-responsive
           RNA helicase [Lycopersicon esculentum] GI:5669638;
           contains Pfam profiles PF00270: DEAD/DEAH box helicase,
           PF00271: Helicase conserved C-terminal domain
          Length = 484

 Score = 90.6 bits (215), Expect = 8e-19
 Identities = 38/89 (42%), Positives = 58/89 (65%)
 Frame = +3

Query: 246 PRLDSVSLQPFNKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPD 425
           P+ +  +L  F KNFY   PTV   +  +V  YR   +++V G +V  P++ F++ANFPD
Sbjct: 115 PKQNFGNLVHFEKNFYVESPTVQAMTEQDVAMYRTERDISVEGRDVPKPMKMFQDANFPD 174

Query: 426 YVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
            + + +  +G+ EPTPIQAQGWP+A+ GR
Sbjct: 175 NILEAIAKLGFTEPTPIQAQGWPMALKGR 203



 Score = 41.1 bits (92), Expect = 6e-04
 Identities = 17/44 (38%), Positives = 28/44 (63%)
 Frame = +2

Query: 509 KNLVGVLKRVPAKRWPTSXPAIVHINNQPPIRRXDGPIALVLAP 640
           ++L+G+ +    K      PA+VH++ QP + + DGPI L+LAP
Sbjct: 203 RDLIGIAETGSGKTLAYLLPALVHVSAQPRLGQDDGPIVLILAP 246


>At2g47330.1 68415.m05908 DEAD/DEAH box helicase, putative similar
           to RNA helicase [Rattus norvegicus] GI:897915; contains
           Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
           Helicase conserved C-terminal domain
          Length = 760

 Score = 73.7 bits (173), Expect = 9e-14
 Identities = 30/85 (35%), Positives = 48/85 (56%)
 Frame = +3

Query: 258 SVSLQPFNKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQ 437
           S+  +P NK+FY+   ++   +  E  +YR    + VSG +VH P++ FE+  F   +  
Sbjct: 182 SIDYEPINKDFYEELESISGMTEQETTDYRQRLGIRVSGFDVHRPVKTFEDCGFSSQIMS 241

Query: 438 GVKTMGYKEPTPIQAQGWPIAMSGR 512
            +K   Y++PT IQ Q  PI +SGR
Sbjct: 242 AIKKQAYEKPTAIQCQALPIVLSGR 266



 Score = 38.7 bits (86), Expect = 0.003
 Identities = 16/44 (36%), Positives = 27/44 (61%)
 Frame = +2

Query: 509 KNLVGVLKRVPAKRWPTSXPAIVHINNQPPIRRXDGPIALVLAP 640
           ++++G+ K    K      P IVHI +QP ++R +GPI ++ AP
Sbjct: 266 RDVIGIAKTGSGKTAAFVLPMIVHIMDQPELQRDEGPIGVICAP 309


>At3g01540.3 68416.m00084 DEAD box RNA helicase (DRH1) identical to
           RNA helicase DRH1 GB:BAA28347 GI:3149952 [Arabidopsis
           thaliana]; contains Pfam profiles PF00270: DEAD/DEAH box
           helicase, PF00271: Helicase conserved C-terminal domain
          Length = 619

 Score = 70.5 bits (165), Expect = 9e-13
 Identities = 33/72 (45%), Positives = 41/72 (56%)
 Frame = +3

Query: 297 PHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPI 476
           P P+    S    E Y   HE+TVSG +V  P+  FE   FP  + + V + G+  PTPI
Sbjct: 125 PLPSSAPASELSPEAYSRRHEITVSGGQVPPPLMSFEATGFPPELLREVLSAGFSAPTPI 184

Query: 477 QAQGWPIAMSGR 512
           QAQ WPIAM GR
Sbjct: 185 QAQSWPIAMQGR 196


>At3g01540.2 68416.m00083 DEAD box RNA helicase (DRH1) identical to
           RNA helicase DRH1 GB:BAA28347 GI:3149952 [Arabidopsis
           thaliana]; contains Pfam profiles PF00270: DEAD/DEAH box
           helicase, PF00271: Helicase conserved C-terminal domain
          Length = 619

 Score = 70.5 bits (165), Expect = 9e-13
 Identities = 33/72 (45%), Positives = 41/72 (56%)
 Frame = +3

Query: 297 PHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPI 476
           P P+    S    E Y   HE+TVSG +V  P+  FE   FP  + + V + G+  PTPI
Sbjct: 125 PLPSSAPASELSPEAYSRRHEITVSGGQVPPPLMSFEATGFPPELLREVLSAGFSAPTPI 184

Query: 477 QAQGWPIAMSGR 512
           QAQ WPIAM GR
Sbjct: 185 QAQSWPIAMQGR 196


>At3g01540.1 68416.m00082 DEAD box RNA helicase (DRH1) identical to
           RNA helicase DRH1 GB:BAA28347 GI:3149952 [Arabidopsis
           thaliana]; contains Pfam profiles PF00270: DEAD/DEAH box
           helicase, PF00271: Helicase conserved C-terminal domain
          Length = 618

 Score = 70.5 bits (165), Expect = 9e-13
 Identities = 33/72 (45%), Positives = 41/72 (56%)
 Frame = +3

Query: 297 PHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPI 476
           P P+    S    E Y   HE+TVSG +V  P+  FE   FP  + + V + G+  PTPI
Sbjct: 125 PLPSSAPASELSPEAYSRRHEITVSGGQVPPPLMSFEATGFPPELLREVLSAGFSAPTPI 184

Query: 477 QAQGWPIAMSGR 512
           QAQ WPIAM GR
Sbjct: 185 QAQSWPIAMQGR 196


>At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative similar
           to RNA helicase GB:A57514 GI:897915 from [Rattus
           norvegicus]; contains Pfam profiles PF00270: DEAD/DEAH
           box helicase, PF00271: Helicase conserved C-terminal
           domain
          Length = 989

 Score = 64.9 bits (151), Expect = 4e-11
 Identities = 30/84 (35%), Positives = 45/84 (53%)
 Frame = +3

Query: 261 VSLQPFNKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQG 440
           +  +PF KNFY     + + +   V  YR   E+ V G +V  PIQ++ +      +   
Sbjct: 351 IEYEPFRKNFYIEVKDISRMTQDAVNAYRKELELKVHGKDVPRPIQFWHQTGLTSKILDT 410

Query: 441 VKTMGYKEPTPIQAQGWPIAMSGR 512
           +K + Y++P PIQAQ  PI MSGR
Sbjct: 411 LKKLNYEKPMPIQAQALPIIMSGR 434



 Score = 40.7 bits (91), Expect = 8e-04
 Identities = 18/44 (40%), Positives = 25/44 (56%)
 Frame = +2

Query: 509 KNLVGVLKRVPAKRWPTSXPAIVHINNQPPIRRXDGPIALVLAP 640
           ++ +GV K    K      P + HI +QPP+   DGPI LV+AP
Sbjct: 434 RDCIGVAKTGSGKTLGFVLPMLRHIKDQPPVEAGDGPIGLVMAP 477


>At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to
           RNA helicase [Rattus norvegicus] GI:897915; contains
           Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
           Helicase conserved C-terminal domain
          Length = 1166

 Score = 64.5 bits (150), Expect = 6e-11
 Identities = 29/84 (34%), Positives = 45/84 (53%)
 Frame = +3

Query: 261 VSLQPFNKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQG 440
           +  +PF KNFY     + + +  EV  YR   E+ V G +V  PI+++ +      +   
Sbjct: 484 IEYEPFRKNFYIEVKDISRMTQEEVNTYRKELELKVHGKDVPRPIKFWHQTGLTSKILDT 543

Query: 441 VKTMGYKEPTPIQAQGWPIAMSGR 512
           +K + Y++P PIQ Q  PI MSGR
Sbjct: 544 MKKLNYEKPMPIQTQALPIIMSGR 567



 Score = 40.7 bits (91), Expect = 8e-04
 Identities = 18/44 (40%), Positives = 25/44 (56%)
 Frame = +2

Query: 509 KNLVGVLKRVPAKRWPTSXPAIVHINNQPPIRRXDGPIALVLAP 640
           ++ +GV K    K      P + HI +QPP+   DGPI LV+AP
Sbjct: 567 RDCIGVAKTGSGKTLGFVLPMLRHIKDQPPVEAGDGPIGLVMAP 610


>At3g06480.1 68416.m00750 DEAD box RNA helicase, putative similar to
           RNA helicase DRH1 [Arabidopsis thaliana] GI:3149952;
           contains Pfam profiles PF00270: DEAD/DEAH box helicase,
           PF00271: Helicase conserved C-terminal domain, PF00397:
           WW domain
          Length = 1088

 Score = 62.9 bits (146), Expect = 2e-10
 Identities = 33/82 (40%), Positives = 44/82 (53%), Gaps = 4/82 (4%)
 Frame = +3

Query: 279 NKNFYDPH----PTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGVK 446
           NK+   PH    P V   SP E+  YR  HEVT +G  +  P   FE +  P  + + + 
Sbjct: 394 NKSLVRPHFVTSPDVPHLSPVEI--YRKQHEVTTTGENIPAPYITFESSGLPPEILRELL 451

Query: 447 TMGYKEPTPIQAQGWPIAMSGR 512
           + G+  PTPIQAQ WPIA+  R
Sbjct: 452 SAGFPSPTPIQAQTWPIALQSR 473


>At4g33370.1 68417.m04744 DEAD-box protein abstrakt, putative RNA
           helicase DBP2 - Saccharomyces cerevisiae, PID:g5272
          Length = 542

 Score = 54.4 bits (125), Expect = 6e-08
 Identities = 26/75 (34%), Positives = 42/75 (56%)
 Frame = +3

Query: 288 FYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEP 467
           ++ P   V K S  +++  R    +TV+G ++  PI+ F +  FP  + + +K  G   P
Sbjct: 61  WWKPPLHVRKMSTKQMDLIRKQWHITVNGEDIPPPIKNFMDMKFPSPLLRMLKDKGIMHP 120

Query: 468 TPIQAQGWPIAMSGR 512
           TPIQ QG P+ +SGR
Sbjct: 121 TPIQVQGLPVVLSGR 135


>At5g51280.1 68418.m06357 DEAD-box protein abstrakt, putative 
          Length = 591

 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 24/74 (32%), Positives = 40/74 (54%)
 Frame = +3

Query: 291 YDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPT 470
           + P   + K S  + +  R    + V+G ++  PI+ F++  FP  V   +K  G  +PT
Sbjct: 111 WKPPLHIRKMSSKQRDLIRKQWHIIVNGDDIPPPIKNFKDMKFPRPVLDTLKEKGIVQPT 170

Query: 471 PIQAQGWPIAMSGR 512
           PIQ QG P+ ++GR
Sbjct: 171 PIQVQGLPVILAGR 184


>At3g09720.1 68416.m01151 DEAD/DEAH box helicase, putative similar
           to RNA helicase involved in rRNA processing GB:6321267
           from [Saccharomyces cerevisiae]c, ontains DEAD and DEAH
           box domain
          Length = 541

 Score = 44.4 bits (100), Expect = 7e-05
 Identities = 22/60 (36%), Positives = 34/60 (56%), Gaps = 4/60 (6%)
 Frame = +3

Query: 345 RNNHEVTVSGVEVHNPIQYFEEANF----PDYVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
           R  + + VSG  +  P++ F E +       Y+ + +  +G+KEPTPIQ Q  PI +SGR
Sbjct: 120 RKQYSIHVSGNNIPPPLKSFAELSSRYGCEGYILRNLAELGFKEPTPIQRQAIPILLSGR 179


>At4g16630.1 68417.m02514 DEAD/DEAH box helicase, putative (RH28)
           identical to cDNA DEAD box RNA helicase, RH28 GI:3776026
          Length = 789

 Score = 42.7 bits (96), Expect = 2e-04
 Identities = 22/50 (44%), Positives = 30/50 (60%)
 Frame = +3

Query: 363 TVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
           TV GV  H     F E N    + +  +T+GYK+PTPIQA   P+A++GR
Sbjct: 158 TVDGVSFH--ADTFMELNLSRPLLRACETLGYKKPTPIQAACIPLALTGR 205


>At2g33730.1 68415.m04134 DEAD box RNA helicase, putative similar to
           SP|P23394 Pre-mRNA splicing factor RNA helicase PRP28
           {Saccharomyces cerevisiae}; contains Pfam profiles
           PF00270: DEAD/DEAH box helicase, PF00271: Helicase
           conserved C-terminal domain
          Length = 733

 Score = 39.1 bits (87), Expect = 0.002
 Identities = 15/57 (26%), Positives = 31/57 (54%)
 Frame = +3

Query: 342 YRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
           +R +  ++  G  +  P++ +EE+     + + V+  GYK+P+PIQ    P+ +  R
Sbjct: 295 FREDFNISYKGSRIPRPMRSWEESKLTSELLKAVERAGYKKPSPIQMAAIPLGLQQR 351


>At3g02065.2 68416.m00170 DEAD/DEAH box helicase family protein
           contains Pfam profile: PF00270 DEAD/DEAH box helicase
          Length = 505

 Score = 38.3 bits (85), Expect = 0.004
 Identities = 29/95 (30%), Positives = 43/95 (45%), Gaps = 3/95 (3%)
 Frame = +3

Query: 249 RLDSVSLQPFNKN-FYDPHPTVLKRSPYEVEEYRNNHEVTVSGV--EVHNPIQYFEEANF 419
           R+DS  + P     FY   P     S ++ +  R   ++ V G    V  P+  F     
Sbjct: 61  RVDSARVFPATDECFYVRDPG---SSSHDAQLLRRKLDIHVQGQGSAVPPPVLTFTSCGL 117

Query: 420 PDYVQQGVKTMGYKEPTPIQAQGWPIAMSGRI*LA 524
           P  +   ++T GY  PTPIQ Q  P A++G+  LA
Sbjct: 118 PPKLLLNLETAGYDFPTPIQMQAIPAALTGKSLLA 152


>At2g42520.1 68415.m05262 DEAD box RNA helicase, putative similar to
           SP|O00571 DEAD-box protein 3 (Helicase-like protein 2)
           {Homo sapiens}, DEAD box RNA helicase DDX3 [Homo
           sapiens] GI:3523150; contains Pfam profiles PF00270:
           DEAD/DEAH box helicase, PF00271: Helicase conserved
           C-terminal domain
          Length = 633

 Score = 37.9 bits (84), Expect = 0.006
 Identities = 19/83 (22%), Positives = 37/83 (44%), Gaps = 1/83 (1%)
 Frame = +3

Query: 267 LQPFNKNFYDPHPTVLKRSPYEVE-EYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGV 443
           + PF  +  +P P   ++    +  +   +  +  SG  V  P+  F E +  + +   +
Sbjct: 115 VNPFENDDSEPEPAFTEQDNTVINFDAYEDIPIETSGDNVPPPVNTFAEIDLGEALNLNI 174

Query: 444 KTMGYKEPTPIQAQGWPIAMSGR 512
           +   Y +PTP+Q    PI + GR
Sbjct: 175 RRCKYVKPTPVQRHAIPILLEGR 197


>At1g31970.1 68414.m03931 DEAD/DEAH box helicase, putative similar
           to p68 RNA helicase [Schizosaccharomyces pombe]
           GI:173419
          Length = 537

 Score = 37.9 bits (84), Expect = 0.006
 Identities = 22/63 (34%), Positives = 34/63 (53%), Gaps = 2/63 (3%)
 Frame = +3

Query: 330 EVEEYRNNHEVTVSGVEV--HNPIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAM 503
           E E  +    VT  GVE   +  ++ F E+N P+ V    KT  +++P+PIQ+  WP  +
Sbjct: 92  EGESEQQKVVVTGKGVEEAKYAALKTFAESNLPENVLDCCKT--FEKPSPIQSHTWPFLL 149

Query: 504 SGR 512
            GR
Sbjct: 150 DGR 152


>At5g14610.1 68418.m01713 DEAD box RNA helicase, putative similar to
           RNA helicase DRH1 [Arabidopsis thaliana] GI:3149952;
           contains Pfam profiles PF00270: DEAD/DEAH box helicase,
           PF00271: Helicase conserved C-terminal domain, PF00397:
           WW domain
          Length = 713

 Score = 35.9 bits (79), Expect = 0.023
 Identities = 14/24 (58%), Positives = 17/24 (70%)
 Frame = +3

Query: 441 VKTMGYKEPTPIQAQGWPIAMSGR 512
           V + G+  P+PIQAQ WPIAM  R
Sbjct: 244 VYSAGFSAPSPIQAQSWPIAMQNR 267



 Score = 31.1 bits (67), Expect = 0.65
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = +3

Query: 336 EEYRNNHEVTVSGVEVHNPIQYFEEANFPD 425
           E Y   HE+TVSG +V  P+  FE    P+
Sbjct: 141 EAYCRKHEITVSGGQVPPPLMSFEATGLPN 170


>At1g77050.1 68414.m08971 DEAD/DEAH box helicase, putative similar
           to RNA helicase GI:3776027 from [Arabidopsis thaliana]
          Length = 513

 Score = 35.9 bits (79), Expect = 0.023
 Identities = 16/36 (44%), Positives = 20/36 (55%)
 Frame = +3

Query: 402 FEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSG 509
           FE  N    V   +K  GYK PTPIQ +  P+ +SG
Sbjct: 30  FESLNLGPNVFNAIKKKGYKVPTPIQRKTMPLILSG 65


>At3g58570.1 68416.m06528 DEAD box RNA helicase, putative similar to
           SP|O00571 DEAD-box protein 3 (Helicase-like protein 2)
           {Homo sapiens}, DEAD box RNA helicase DDX3 [Homo
           sapiens] GI:3523150; contains Pfam profiles PF00270:
           DEAD/DEAH box helicase, PF00271: Helicase conserved
           C-terminal domain
          Length = 646

 Score = 34.7 bits (76), Expect = 0.053
 Identities = 22/81 (27%), Positives = 35/81 (43%), Gaps = 1/81 (1%)
 Frame = +3

Query: 273 PF-NKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGVKT 449
           PF N    DP     + +    E Y +   +  SG  V  P+  F E +  + +   ++ 
Sbjct: 105 PFGNDGNADPAVNEQENTVINFEAYEDI-PIETSGDNVPPPVNTFAEIDLGEALNLNIQR 163

Query: 450 MGYKEPTPIQAQGWPIAMSGR 512
             Y +PTP+Q    PI  +GR
Sbjct: 164 CKYVKPTPVQRNAIPILAAGR 184


>At3g58510.2 68416.m06522 DEAD box RNA helicase, putative (RH11)
           similar to RNA helicase DBY protein [Mus musculus]
           GI:3790186, SP|O00571 DEAD-box protein 3 (Helicase-like
           protein 2) {Homo sapiens}; contains Pfam profiles
           PF00270: DEAD/DEAH box helicase, PF00271: Helicase
           conserved C-terminal domain; identical to cDNA DEAD box
           RNA helicase, RH11 GI:3775998
          Length = 612

 Score = 33.5 bits (73), Expect = 0.12
 Identities = 15/51 (29%), Positives = 25/51 (49%)
 Frame = +3

Query: 360 VTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
           V  SG +V  P+  F + +  D +   ++   Y  PTP+Q    PI ++ R
Sbjct: 139 VETSGGDVPPPVNTFADIDLGDALNLNIRRCKYVRPTPVQRHAIPILLAER 189


>At3g58510.1 68416.m06521 DEAD box RNA helicase, putative (RH11)
           similar to RNA helicase DBY protein [Mus musculus]
           GI:3790186, SP|O00571 DEAD-box protein 3 (Helicase-like
           protein 2) {Homo sapiens}; contains Pfam profiles
           PF00270: DEAD/DEAH box helicase, PF00271: Helicase
           conserved C-terminal domain; identical to cDNA DEAD box
           RNA helicase, RH11 GI:3775998
          Length = 612

 Score = 33.5 bits (73), Expect = 0.12
 Identities = 15/51 (29%), Positives = 25/51 (49%)
 Frame = +3

Query: 360 VTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
           V  SG +V  P+  F + +  D +   ++   Y  PTP+Q    PI ++ R
Sbjct: 139 VETSGGDVPPPVNTFADIDLGDALNLNIRRCKYVRPTPVQRHAIPILLAER 189


>At1g51380.1 68414.m05780 eukaryotic translation initiation factor
           4A, putative / eIF-4A, putative
          Length = 392

 Score = 32.3 bits (70), Expect = 0.28
 Identities = 15/41 (36%), Positives = 23/41 (56%)
 Frame = +3

Query: 390 PIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
           PI+ F++    D V +GV   GYK+P+ IQ +     + GR
Sbjct: 20  PIKSFDDMGMNDKVLRGVYDYGYKKPSEIQQRALVPILKGR 60


>At4g00660.2 68417.m00091 DEAD/DEAH box helicase, putative similar
           to ATP-dependent RNA helicases
          Length = 505

 Score = 31.9 bits (69), Expect = 0.37
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = +3

Query: 402 FEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGRI*LA 524
           FE+      +  G+   G++ P+PIQ +  PIA++GR  LA
Sbjct: 133 FEDYFLKRELLMGIYEKGFERPSPIQEESIPIALTGRDILA 173


>At4g00660.1 68417.m00090 DEAD/DEAH box helicase, putative similar
           to ATP-dependent RNA helicases
          Length = 505

 Score = 31.9 bits (69), Expect = 0.37
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = +3

Query: 402 FEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGRI*LA 524
           FE+      +  G+   G++ P+PIQ +  PIA++GR  LA
Sbjct: 133 FEDYFLKRELLMGIYEKGFERPSPIQEESIPIALTGRDILA 173


>At4g34910.1 68417.m04950 DEAD/DEAH box helicase, putative (RH16)
           identical to cDNA DEAD box RNA helicase, RH16 GI:3776006
          Length = 626

 Score = 31.1 bits (67), Expect = 0.65
 Identities = 19/61 (31%), Positives = 28/61 (45%)
 Frame = +3

Query: 330 EVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSG 509
           EVEE RN+ E      E   P + FEE      + + +   G ++PT IQ    P  + G
Sbjct: 25  EVEEQRNDREQEEEQKEEEAP-KSFEELGLDSRLIRALTKKGIEKPTLIQQSAIPYILEG 83

Query: 510 R 512
           +
Sbjct: 84  K 84



 Score = 27.5 bits (58), Expect = 8.0
 Identities = 11/14 (78%), Positives = 13/14 (92%)
 Frame = +1

Query: 523 RTQTGSGKTLAYIL 564
           R +TGSGKTLAY+L
Sbjct: 89  RAKTGSGKTLAYLL 102


>At5g60990.1 68418.m07651 DEAD/DEAH box helicase, putative (RH10)
           probable replication protein A1, Oryza sativa,
           EMBL:AF009179
          Length = 456

 Score = 30.3 bits (65), Expect = 1.1
 Identities = 12/45 (26%), Positives = 25/45 (55%)
 Frame = +3

Query: 378 EVHNPIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
           E +  ++ F E    + + +  + +G+K P+ IQA+  P A+ G+
Sbjct: 3   EENEVVKTFAELGVREELVKACERLGWKNPSKIQAEALPFALEGK 47


>At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contains
           Pfam profiles  PF00270:DEAD/DEAH box helicase and
           PF00271:  Helicase conserved C-terminal domain;
           identical to cDNA RH27 helicase, partial GI:4033334
          Length = 633

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 20/80 (25%), Positives = 32/80 (40%)
 Frame = +3

Query: 270 QPFNKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQQGVKT 449
           +P  K       T  K    EVE+     ++  + +  +     FE  +  D   + +K 
Sbjct: 115 EPKKKKKKQRKDTEAKSEEEEVEDKEEEKKLEETSIMTNKT---FESLSLSDNTYKSIKE 171

Query: 450 MGYKEPTPIQAQGWPIAMSG 509
           MG+   T IQA+  P  M G
Sbjct: 172 MGFARMTQIQAKAIPPLMMG 191


>At3g61240.2 68416.m06854 DEAD/DEAH box helicase, putative (RH12)
           identical to cDNA DEAD box RNA helicase, RH12 GI:3776000
          Length = 498

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 12/36 (33%), Positives = 23/36 (63%)
 Frame = +3

Query: 402 FEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSG 509
           FE+      + +G+   G+++P+PIQ +  PIA++G
Sbjct: 126 FEDYFLKRDLLKGIYEKGFEKPSPIQEESIPIALTG 161


>At3g61240.1 68416.m06853 DEAD/DEAH box helicase, putative (RH12)
           identical to cDNA DEAD box RNA helicase, RH12 GI:3776000
          Length = 498

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 12/36 (33%), Positives = 23/36 (63%)
 Frame = +3

Query: 402 FEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSG 509
           FE+      + +G+   G+++P+PIQ +  PIA++G
Sbjct: 126 FEDYFLKRDLLKGIYEKGFEKPSPIQEESIPIALTG 161


>At2g45810.1 68415.m05697 DEAD/DEAH box helicase, putative
          Length = 528

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 12/36 (33%), Positives = 23/36 (63%)
 Frame = +3

Query: 402 FEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSG 509
           FE+      + +G+   G+++P+PIQ +  PIA++G
Sbjct: 156 FEDYFLKRDLLRGIYEKGFEKPSPIQEESIPIALTG 191


>At2g40700.1 68415.m05021 DEAD/DEAH box helicase, putative (RH17)
           identical to GB:CAA09207, contains a DEAD/DEAH box
           family ATP-dependent helicas signature; identical to
           cDNA DEAD box RNA helicase, RH17 GI:3776008
          Length = 609

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 11/21 (52%), Positives = 16/21 (76%)
 Frame = +3

Query: 450 MGYKEPTPIQAQGWPIAMSGR 512
           MG++ PT +QAQ  P+ +SGR
Sbjct: 48  MGFEAPTLVQAQAIPVILSGR 68


>At5g43990.2 68418.m05382 SET domain-containing protein identical to
           SET domain protein SUVR2 [Arabidopsis thaliana]
           GI:15290521; contains Pfam profiles PF00856: SET domain,
           PF05033: Pre-SET motif; identical to cDNA SET domain
           protein SUVR2 GI:15290520
          Length = 740

 Score = 28.7 bits (61), Expect = 3.5
 Identities = 21/71 (29%), Positives = 35/71 (49%)
 Frame = +3

Query: 240 ASPRLDSVSLQPFNKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANF 419
           ASP L S +L+  + N  +  P +L   P  +E   +  E+ ++ VE   PI     ++ 
Sbjct: 146 ASPSLGSPTLEGPSINDEENAPILLPYHPVPIENDHDAGELILTKVE---PITNMPLSSI 202

Query: 420 PDYVQQGVKTM 452
           PD V +G  +M
Sbjct: 203 PDSVDRGDSSM 213


>At5g43990.1 68418.m05383 SET domain-containing protein identical to
           SET domain protein SUVR2 [Arabidopsis thaliana]
           GI:15290521; contains Pfam profiles PF00856: SET domain,
           PF05033: Pre-SET motif; identical to cDNA SET domain
           protein SUVR2 GI:15290520
          Length = 717

 Score = 28.7 bits (61), Expect = 3.5
 Identities = 21/71 (29%), Positives = 35/71 (49%)
 Frame = +3

Query: 240 ASPRLDSVSLQPFNKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANF 419
           ASP L S +L+  + N  +  P +L   P  +E   +  E+ ++ VE   PI     ++ 
Sbjct: 123 ASPSLGSPTLEGPSINDEENAPILLPYHPVPIENDHDAGELILTKVE---PITNMPLSSI 179

Query: 420 PDYVQQGVKTM 452
           PD V +G  +M
Sbjct: 180 PDSVDRGDSSM 190


>At3g49500.1 68416.m05410 RNA-dependent RNA polymerase (SDE1)
           identical to RNA-dependent RNA polymerase [Arabidopsis
           thaliana] gi|8248473|gb|AAF74208
          Length = 1196

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 10/31 (32%), Positives = 18/31 (58%)
 Frame = +2

Query: 185 LEDLEDLVGKKNSLEVRTCVAQIGFCFTPTF 277
           + D++  +GK     V  C A++G CF+ T+
Sbjct: 530 VSDIKTWMGKFKDKNVAKCAARMGLCFSSTY 560


>At2g33420.1 68415.m04096 expressed protein
          Length = 1039

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 12/17 (70%), Positives = 13/17 (76%)
 Frame = +3

Query: 24  SKRIHSLNKHLQLNPKI 74
           S  IHSLNK L LNP+I
Sbjct: 789 SSHIHSLNKTLSLNPRI 805


>At2g02440.1 68415.m00183 hypothetical protein
          Length = 138

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = -1

Query: 597 GGWLFMCTMAGQDVGQRFAGTRLSTPTKF 511
           GG+  +C+ +G+D G++F  +    P KF
Sbjct: 29  GGYHSVCSTSGEDGGEKFCSSSKPKPKKF 57


>At1g16280.1 68414.m01949 DEAD/DEAH box helicase, putative similar
           to gb|L13612 DEAD-box protein (dbp45A) from Drosophila
           melanogaster and is a member of PF|00270 DEAD/DEAH box
           helicase family
          Length = 491

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 11/37 (29%), Positives = 20/37 (54%)
 Frame = +3

Query: 402 FEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSGR 512
           FE     ++  +  K +G ++PTP+Q    P  ++GR
Sbjct: 60  FEGLGLAEWAVETCKELGMRKPTPVQTHCVPKILAGR 96


>At1g12770.1 68414.m01482 DEAD/DEAH box helicase family protein /
           pentatricopeptide (PPR) repeat-containing protein
           contains Pfam profiles:  PF00271 helicase conserved
           C-terminal domain, PF01535 PPR repeat, PF00270:
           DEAD/DEAH box helicase
          Length = 1145

 Score = 27.9 bits (59), Expect = 6.1
 Identities = 11/36 (30%), Positives = 18/36 (50%)
 Frame = +3

Query: 402 FEEANFPDYVQQGVKTMGYKEPTPIQAQGWPIAMSG 509
           FEE   PD +   ++  G+  PT +Q+   P  + G
Sbjct: 112 FEELGLPDSLLDSLEREGFSVPTDVQSAAVPAIIKG 147


>At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes
           from this gene
          Length = 1035

 Score = 27.9 bits (59), Expect = 6.1
 Identities = 10/17 (58%), Positives = 14/17 (82%)
 Frame = +3

Query: 24  SKRIHSLNKHLQLNPKI 74
           S ++HSLNK L LNP++
Sbjct: 786 SSQLHSLNKSLSLNPRV 802


>At2g36300.1 68415.m04455 integral membrane Yip1 family protein
           contains Pfam domain, PF04893: Yip1 domain
          Length = 255

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 8/23 (34%), Positives = 14/23 (60%)
 Frame = +2

Query: 203 LVGKKNSLEVRTCVAQIGFCFTP 271
           L G+  +L + TC + +G+C  P
Sbjct: 164 LAGRNGNLNLHTCTSLVGYCLLP 186


>At2g25460.1 68415.m03049 expressed protein
          Length = 423

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 8/22 (36%), Positives = 14/22 (63%)
 Frame = -3

Query: 160 ETNYRRICCLLQIWNHRFHGYY 95
           E  + R+CC++  WN  F+ +Y
Sbjct: 80  EEEFERVCCIVGPWNLSFNVFY 101


>At2g01830.3 68415.m00115 histidine kinase (AHK4) (WOL) identical to
           histidine kinase AHK4 [Arabidopsis thaliana]
           gi|13537200|dbj|BAB40776; contains Pfam profiles
           PF03924: CHASE domain,  PF02518:  ATPase, histidine
           kinase-, DNA gyrase B-, and HSP90-like domain protein,
           PF00512:  His Kinase A (phosphoacceptor) domain,
           PF00072:  Response regulator receiver domain
          Length = 1057

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 18/73 (24%), Positives = 31/73 (42%), Gaps = 1/73 (1%)
 Frame = +3

Query: 291 YDPHPTVLKRSPYEVEEYRNNHE-VTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEP 467
           Y  +P+ + +  +     R   E   +SGV     +  FE   F       +KTM   EP
Sbjct: 172 YHKNPSAIDQETFAEYTARTAFERPLLSGVAYAEKVVNFEREMFERQHNWVIKTMDRGEP 231

Query: 468 TPIQAQGWPIAMS 506
           +P++ +  P+  S
Sbjct: 232 SPVRDEYAPVIFS 244


>At2g01830.2 68415.m00116 histidine kinase (AHK4) (WOL) identical to
           histidine kinase AHK4 [Arabidopsis thaliana]
           gi|13537200|dbj|BAB40776; contains Pfam profiles
           PF03924: CHASE domain,  PF02518:  ATPase, histidine
           kinase-, DNA gyrase B-, and HSP90-like domain protein,
           PF00512:  His Kinase A (phosphoacceptor) domain,
           PF00072:  Response regulator receiver domain
          Length = 1080

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 18/73 (24%), Positives = 31/73 (42%), Gaps = 1/73 (1%)
 Frame = +3

Query: 291 YDPHPTVLKRSPYEVEEYRNNHE-VTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEP 467
           Y  +P+ + +  +     R   E   +SGV     +  FE   F       +KTM   EP
Sbjct: 195 YHKNPSAIDQETFAEYTARTAFERPLLSGVAYAEKVVNFEREMFERQHNWVIKTMDRGEP 254

Query: 468 TPIQAQGWPIAMS 506
           +P++ +  P+  S
Sbjct: 255 SPVRDEYAPVIFS 267


>At2g01830.1 68415.m00114 histidine kinase (AHK4) (WOL) identical to
           histidine kinase AHK4 [Arabidopsis thaliana]
           gi|13537200|dbj|BAB40776; contains Pfam profiles
           PF03924: CHASE domain,  PF02518:  ATPase, histidine
           kinase-, DNA gyrase B-, and HSP90-like domain protein,
           PF00512:  His Kinase A (phosphoacceptor) domain,
           PF00072:  Response regulator receiver domain
          Length = 1057

 Score = 27.5 bits (58), Expect = 8.0
 Identities = 18/73 (24%), Positives = 31/73 (42%), Gaps = 1/73 (1%)
 Frame = +3

Query: 291 YDPHPTVLKRSPYEVEEYRNNHE-VTVSGVEVHNPIQYFEEANFPDYVQQGVKTMGYKEP 467
           Y  +P+ + +  +     R   E   +SGV     +  FE   F       +KTM   EP
Sbjct: 172 YHKNPSAIDQETFAEYTARTAFERPLLSGVAYAEKVVNFEREMFERQHNWVIKTMDRGEP 231

Query: 468 TPIQAQGWPIAMS 506
           +P++ +  P+  S
Sbjct: 232 SPVRDEYAPVIFS 244


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,673,021
Number of Sequences: 28952
Number of extensions: 245696
Number of successful extensions: 772
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 728
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 765
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -