BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060692.seq (636 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 118 9e-28 SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 80 3e-16 SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 27 2.3 SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 27 3.0 SPBC16D10.03 |pgp2||metallopeptidase Pgp2|Schizosaccharomyces po... 26 4.0 SPCC16A11.13 |usp106|luc7|U1 snRNP-associated protein Usp106|Sch... 26 5.2 SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 26 5.2 SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 25 9.1 SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccha... 25 9.1 >SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr 1|||Manual Length = 778 Score = 118 bits (283), Expect = 9e-28 Identities = 57/86 (66%), Positives = 69/86 (80%), Gaps = 1/86 (1%) Frame = +1 Query: 265 QVAMSKFDKVP-LPYEKLTKNLEVVKKRLGRELTLSEKILYSHLDDPKGQEIERGASYLR 441 +VAMS F+K + Y+++ NLE+VKKRL R LT SEKILY HLDDP Q+IERG SYL+ Sbjct: 27 KVAMSNFEKNKFINYQRIKDNLEIVKKRLNRPLTYSEKILYGHLDDPVNQDIERGVSYLK 86 Query: 442 LRPDRVAMQDATAQMAMLQFISSAFP 519 LRPDRVA QDATAQMA+LQF+S+ P Sbjct: 87 LRPDRVACQDATAQMAILQFMSAGMP 112 Score = 62.5 bits (145), Expect = 5e-11 Identities = 28/42 (66%), Positives = 31/42 (73%) Frame = +3 Query: 510 GLPRVAVPSTIHCDHLIEAQVGGEKDLARAKDLNKEVYKFLE 635 G+P VAVP T+HCDHLIEA GG DL RA NKEVY FL+ Sbjct: 110 GMPEVAVPVTVHCDHLIEAYEGGPIDLERANVTNKEVYDFLQ 151 >SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 79.8 bits (188), Expect = 3e-16 Identities = 46/90 (51%), Positives = 61/90 (67%), Gaps = 7/90 (7%) Frame = +1 Query: 259 RAQVAMSKFDKVPLPYEKLTKNLEVVKKRL-GRELTLSEKILYSHLDDPK------GQEI 417 R Q S+ V PYEKL L+ V+K L G++LTL+EK+LYSHL +P+ Sbjct: 23 RFQSTASRASYVTPPYEKLMGKLQQVRKFLPGQKLTLAEKVLYSHLVNPEESFSGVSPSD 82 Query: 418 ERGASYLRLRPDRVAMQDATAQMAMLQFIS 507 RG+ YL+L PDRVAMQDA+AQMA+LQF++ Sbjct: 83 IRGSLYLKLNPDRVAMQDASAQMALLQFMT 112 Score = 43.2 bits (97), Expect = 3e-05 Identities = 17/42 (40%), Positives = 26/42 (61%) Frame = +3 Query: 510 GLPRVAVPSTIHCDHLIEAQVGGEKDLARAKDLNKEVYKFLE 635 GL + +P++IHCDHLI G D+ + NKE++ FL+ Sbjct: 114 GLEKTMIPASIHCDHLIVGHRGANSDIPDSIANNKEIFDFLQ 155 >SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1183 Score = 27.1 bits (57), Expect = 2.3 Identities = 21/80 (26%), Positives = 35/80 (43%) Frame = +1 Query: 199 QGWYSLKSNKDVSAYLH*PRRAQVAMSKFDKVPLPYEKLTKNLEVVKKRLGRELTLSEKI 378 +G S K K + Y+ + + S K L E++ +L K LG++ T EK+ Sbjct: 81 EGSISSKKKKRLDKYIENKLKKEEVASLIAK--LAEERIDTSLLSSSKNLGKQATAKEKL 138 Query: 379 LYSHLDDPKGQEIERGASYL 438 S L++ G + S L Sbjct: 139 KKSLLEEKLGLPLSDADSRL 158 >SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 339 Score = 26.6 bits (56), Expect = 3.0 Identities = 11/28 (39%), Positives = 14/28 (50%) Frame = -2 Query: 284 NLDIATWARRGQWRYAETSLLDFREYHP 201 N D TWA +WR + SL+ HP Sbjct: 44 NDDTETWAITSEWRAGDASLMRVAWAHP 71 >SPBC16D10.03 |pgp2||metallopeptidase Pgp2|Schizosaccharomyces pombe|chr 2|||Manual Length = 346 Score = 26.2 bits (55), Expect = 4.0 Identities = 11/23 (47%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Frame = -3 Query: 283 TWTSPPGRGGVNGDTLK-HLCWI 218 T+ +PPG+G + DT K H WI Sbjct: 39 TYITPPGQGFLPSDTAKHHRAWI 61 >SPCC16A11.13 |usp106|luc7|U1 snRNP-associated protein Usp106|Schizosaccharomyces pombe|chr 3|||Manual Length = 264 Score = 25.8 bits (54), Expect = 5.2 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = +3 Query: 540 IHCDHLIEAQVGGEKDLARAKDLNKEVYKFLE 635 IH DH IE + + LA+ K +N V F+E Sbjct: 124 IHTDHSIEVIITEMEALAKRKLVNDAVKHFIE 155 >SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pombe|chr 1|||Manual Length = 923 Score = 25.8 bits (54), Expect = 5.2 Identities = 9/23 (39%), Positives = 16/23 (69%) Frame = +1 Query: 277 SKFDKVPLPYEKLTKNLEVVKKR 345 +KFD+V +PY+ + ++E KR Sbjct: 396 AKFDEVDMPYDTIWLDIEYASKR 418 >SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac1|Schizosaccharomyces pombe|chr 2|||Manual Length = 384 Score = 25.0 bits (52), Expect = 9.1 Identities = 15/42 (35%), Positives = 20/42 (47%) Frame = -3 Query: 235 KHLCWISESTTLVLLPWPCKTLIQCAMVAGLQYIKRINLEKF 110 ++ CWIS TL+LL T +Q + L I RI F Sbjct: 320 QYKCWISRDVTLILL-----TALQLVNIYWLILILRIGYRAF 356 >SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccharomyces pombe|chr 1|||Manual Length = 944 Score = 25.0 bits (52), Expect = 9.1 Identities = 10/28 (35%), Positives = 15/28 (53%) Frame = +2 Query: 443 CVPTVWPCKTPLHKWQCYNLSLRPSPCS 526 C+P+ P TP+ + Y L+ P CS Sbjct: 11 CIPSSSPPSTPIREENYYLLTSSPIECS 38 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,704,449 Number of Sequences: 5004 Number of extensions: 54186 Number of successful extensions: 137 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 136 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 283719918 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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