Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060691.seq
(627 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|... 144 7e-36
SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 72 6e-14
SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2... 59 4e-10
SPBC25B2.04c |||mitochondrial ribosome assembly protein|Schizosa... 43 3e-05
SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyce... 37 0.002
SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa... 34 0.015
SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual 31 0.18
SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom... 30 0.31
SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 29 0.73
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 29 0.73
SPBC354.01 |gtp1|SPBC649.06|GTP binding protein Gtp1|Schizosacch... 28 0.96
SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr 1|||M... 28 0.96
SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 28 1.3
SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces po... 27 2.9
SPAP8A3.03 |||ZIP zinc transporter 1|Schizosaccharomyces pombe|c... 27 2.9
SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 26 5.1
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 25 9.0
SPCC1183.07 |||U3 snoRNP-associated protein Rrp5|Schizosaccharom... 25 9.0
SPAC16E8.14c |||methyltransferase |Schizosaccharomyces pombe|chr... 25 9.0
>SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 537
Score = 144 bits (350), Expect = 7e-36
Identities = 65/81 (80%), Positives = 70/81 (86%)
Frame = +1
Query: 13 FGKGSLINLLRQFGKLHIDKKQISVGFIGYPNVGKSSVINTLRSKKVCKVAPIAGETKVW 192
FGKGSLI +LRQF LH DKKQISVG IG+PN GKSS+INTLR KKVC VAPI GETKVW
Sbjct: 289 FGKGSLIQILRQFASLHSDKKQISVGLIGFPNAGKSSIINTLRKKKVCNVAPIPGETKVW 348
Query: 193 QYITLMKRIFLIDCPGVVYPS 255
QY+ LMKRIFLIDCPG+V PS
Sbjct: 349 QYVALMKRIFLIDCPGIVPPS 369
Score = 100 bits (240), Expect = 1e-22
Identities = 46/84 (54%), Positives = 62/84 (73%)
Frame = +3
Query: 258 ETDTEKVLKGVVRVELVQNPDDYIEEVIKRVRKEYLVKTYKVDGWETATEFLEKLAARTG 437
++D E +LKGVVRVE V NP+ YI V+ R + ++L +TY++ GW +TEFL KLA + G
Sbjct: 372 DSDAELLLKGVVRVENVSNPEAYIPTVLSRCKVKHLERTYEISGWNDSTEFLAKLAKKGG 431
Query: 438 KLLKKGEPDVNQVARMVLNDWQRG 509
+LLK GEPD VA+MVLND+ RG
Sbjct: 432 RLLKGGEPDEASVAKMVLNDFMRG 455
>SPAC3F10.16c |||GTP binding protein,
HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual
Length = 616
Score = 72.1 bits (169), Expect = 6e-14
Identities = 32/67 (47%), Positives = 45/67 (67%)
Frame = +1
Query: 70 KKQISVGFIGYPNVGKSSVINTLRSKKVCKVAPIAGETKVWQYITLMKRIFLIDCPGVVY 249
K +++ G +GYPNVGKSS IN L K V+ G+TK +Q I L +++ L+DCPG+V+
Sbjct: 299 KTKMTFGLVGYPNVGKSSTINALVGSKKVSVSSTPGKTKHFQTINLSEKVSLLDCPGLVF 358
Query: 250 PSLKQTQ 270
PS TQ
Sbjct: 359 PSFATTQ 365
>SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 470
Score = 59.3 bits (137), Expect = 4e-10
Identities = 36/95 (37%), Positives = 51/95 (53%), Gaps = 10/95 (10%)
Frame = +1
Query: 1 RHEPFGKGSLI-NLLRQFGKLHIDKK---QISVGFIGYPNVGKSSVINTLRSKKV----- 153
+H+ S I NLL+ KK ++VG IGYPNVGKSSVIN L ++
Sbjct: 240 KHQSASASSTISNLLKSLKSYSAKKKLKSSLTVGVIGYPNVGKSSVINALVNRSANGRSA 299
Query: 154 -CKVAPIAGETKVWQYITLMKRIFLIDCPGVVYPS 255
C +AG T + + L ++ L+D PG+V+PS
Sbjct: 300 PCPAGNVAGMTTSLREVKLDNKLRLVDSPGIVFPS 334
Score = 45.2 bits (102), Expect = 8e-06
Identities = 22/61 (36%), Positives = 36/61 (59%)
Frame = +3
Query: 327 IEEVIKRVRKEYLVKTYKVDGWETATEFLEKLAARTGKLLKKGEPDVNQVARMVLNDWQR 506
+E + +R L+ T +D TAT+FL +A + G+L + G P++N A +V+NDW
Sbjct: 376 LERMFQRYELPPLLNTSDID---TATDFLVNIARKRGRLGRGGIPNLNAAANIVINDWHA 432
Query: 507 G 509
G
Sbjct: 433 G 433
>SPBC25B2.04c |||mitochondrial ribosome assembly
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 328
Score = 43.2 bits (97), Expect = 3e-05
Identities = 27/95 (28%), Positives = 49/95 (51%), Gaps = 8/95 (8%)
Frame = +1
Query: 10 PFGKGSLINLLRQFGKLHIDKKQISVGFIGYPNVGKSSVINTLRS-----KKVCKVAPIA 174
P+ L+ L+R ++ V F+G PN GKSS++N+LR+ K V
Sbjct: 123 PYFAKELLRLIRTLVDQASANGRVYVYFVGMPNTGKSSILNSLRNVALRKSKSAIVGNYP 182
Query: 175 GETK-VWQYITLMK--RIFLIDCPGVVYPSLKQTQ 270
G TK + + + L ++++D PG++ PS+ + +
Sbjct: 183 GVTKRISEIVRLFNDMDVYMLDTPGIMTPSITKPE 217
>SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 37.1 bits (82), Expect = 0.002
Identities = 16/39 (41%), Positives = 27/39 (69%)
Frame = +1
Query: 70 KKQISVGFIGYPNVGKSSVINTLRSKKVCKVAPIAGETK 186
+K I+V +G N GKSS+IN L ++++ V+P +G T+
Sbjct: 237 RKGINVAILGPSNAGKSSLINLLANRRISIVSPQSGTTR 275
>SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 34.3 bits (75), Expect = 0.015
Identities = 26/70 (37%), Positives = 36/70 (51%), Gaps = 3/70 (4%)
Frame = +1
Query: 64 IDKKQISVGFIGYPNVGKSSVINTLRSKKVCKVAPIAGETK---VWQYITLMKRIFLIDC 234
ID ++ GYPNVGKSS +N + +V V P A TK V + R +ID
Sbjct: 164 IDPNTRTLLVCGYPNVGKSSFMNKVTRAQV-DVQPYAFTTKSLFVGHFDYKYLRWQVIDT 222
Query: 235 PGVVYPSLKQ 264
PG++ L+Q
Sbjct: 223 PGILDHPLEQ 232
>SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 366
Score = 30.7 bits (66), Expect = 0.18
Identities = 20/59 (33%), Positives = 34/59 (57%), Gaps = 4/59 (6%)
Frame = +1
Query: 82 SVGFIGYPNVGKSSVINTL---RSKKVC-KVAPIAGETKVWQYITLMKRIFLIDCPGVV 246
+VGFIG+P+VGKS+++ L RS+ + + V QY +I ++D PG++
Sbjct: 65 TVGFIGFPSVGKSTLMTQLTGTRSEAAAYEFTTLTTVPGVLQYNG--AKIQILDLPGII 121
>SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 419
Score = 29.9 bits (64), Expect = 0.31
Identities = 12/32 (37%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
Frame = +1
Query: 58 LHIDKKQI-SVGFIGYPNVGKSSVINTLRSKK 150
+ ++ K I +G +G PN GKS+++N L + K
Sbjct: 229 IELELKTICEIGLVGLPNAGKSTLLNCLTASK 260
>SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 409
Score = 28.7 bits (61), Expect = 0.73
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = +1
Query: 73 KQISVGFIGYPNVGKSSVINTL 138
+ I +GF+G P+ GKS+++N L
Sbjct: 3 RDILIGFVGKPSSGKSTMLNAL 24
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 28.7 bits (61), Expect = 0.73
Identities = 14/50 (28%), Positives = 30/50 (60%)
Frame = +1
Query: 28 LINLLRQFGKLHIDKKQISVGFIGYPNVGKSSVINTLRSKKVCKVAPIAG 177
L+ +LRQ + ++++ V +G N GK++++ L ++ V +V+P G
Sbjct: 3 LLTILRQ---QKLKEREVRVLLLGLDNAGKTTILKCLLNEDVNEVSPTFG 49
>SPBC354.01 |gtp1|SPBC649.06|GTP binding protein
Gtp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 28.3 bits (60), Expect = 0.96
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = +1
Query: 85 VGFIGYPNVGKSSVINTLRSKK 150
V FIG+P+VGKS++++ + K
Sbjct: 65 VAFIGFPSVGKSTLLSAITKTK 86
>SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 407
Score = 28.3 bits (60), Expect = 0.96
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = +1
Query: 13 FGKGSLINLLRQFGKLHIDKKQISVGFIGYPNVGKSSVINTL 138
+ K I+++RQ +L + +G +G PN+GKS++ L
Sbjct: 26 YSKQKDISIVRQ--RLGRPGNHLKIGIVGMPNIGKSTLFQIL 65
>SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 754
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +3
Query: 297 VELVQNPDDYIEEVIKRVRKEYLVKTYK 380
+ VQN D +++I R R++YL + YK
Sbjct: 80 ITFVQNVTDIDDKIILRARQQYLFEEYK 107
>SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 373
Score = 26.6 bits (56), Expect = 2.9
Identities = 20/73 (27%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Frame = +3
Query: 306 VQNPDDYIEEVIKRV--RKEYLVKTYKVDGWETATEFLEKLAARTGKL-LKKGEPDVNQV 476
V+NP +E +++ R+E + T + A G+L KKG D+ V
Sbjct: 187 VENPQSTKDEPLQKTNQRQESNLATSPASTVSRVRALYDFAATEQGELSFKKG--DIILV 244
Query: 477 ARMVLNDWQRGNC 515
V DW +G+C
Sbjct: 245 LESVYKDWWKGSC 257
>SPAP8A3.03 |||ZIP zinc transporter 1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 453
Score = 26.6 bits (56), Expect = 2.9
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -3
Query: 547 YFKSLWSYIERQFPLCQSLRTILATWLTSGSP 452
+ S+ S++E+QF ILAT+LT+ P
Sbjct: 119 WLNSVKSFVEKQFSYSSGTNGILATFLTAIPP 150
>SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit
Rpt6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 403
Score = 25.8 bits (54), Expect = 5.1
Identities = 12/28 (42%), Positives = 19/28 (67%), Gaps = 1/28 (3%)
Frame = +3
Query: 297 VELVQNPDDYIEEVIKRVRK-EYLVKTY 377
++L+Q P Y+ EVIK + K + LVK +
Sbjct: 59 IQLLQEPGSYVGEVIKTMGKNKVLVKVH 86
>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 762
Score = 25.0 bits (52), Expect = 9.0
Identities = 25/79 (31%), Positives = 34/79 (43%), Gaps = 10/79 (12%)
Frame = +3
Query: 246 LSIAETDTEKVL-----KGVVR-VELVQNPDDYIEEVIKRVRKEYLVKTYKVDGWETA-- 401
+S+A D K + KG V VEL+ + EV K V K +D A
Sbjct: 275 ISVANPDVRKEIYMEGHKGTVEEVELLNSYLRSKAEVAKLVGKSSFADLQLIDKMANAPK 334
Query: 402 --TEFLEKLAARTGKLLKK 452
EFLE L+ + +LKK
Sbjct: 335 HVVEFLENLSLKNSSVLKK 353
>SPCC1183.07 |||U3 snoRNP-associated protein
Rrp5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1690
Score = 25.0 bits (52), Expect = 9.0
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 94 IGYPNVGKSSVINTLRSKKVCKVAPIAGETKV 189
+G P + + I+ L KKV ++P +G KV
Sbjct: 419 VGVPEISGFAHISRLSDKKVAGISPNSGPYKV 450
>SPAC16E8.14c |||methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 219
Score = 25.0 bits (52), Expect = 9.0
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +3
Query: 201 HLNEENILD*LSGCCLSIAETDTEKVLKGVVRVELVQNPDD 323
HL +E+++ LS CC +I E V + V E +P D
Sbjct: 132 HLTDEDLIAYLSRCCEAIQEKGVICVKENVSSFEDTFDPID 172
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,388,617
Number of Sequences: 5004
Number of extensions: 47433
Number of successful extensions: 164
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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