Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060688.seq
(637 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g39520.1 68417.m05588 GTP-binding protein, putative similar t... 121 5e-28
At1g17470.1 68414.m02143 developmentally regulated GTP-binding p... 117 6e-27
At1g72660.1 68414.m08403 developmentally regulated GTP-binding p... 116 1e-26
At5g43060.1 68418.m05256 cysteine proteinase, putative / thiol p... 28 4.5
At4g31630.1 68417.m04493 transcriptional factor B3 family protei... 28 6.0
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 28 6.0
At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-... 27 7.9
At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-... 27 7.9
At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative 27 7.9
>At4g39520.1 68417.m05588 GTP-binding protein, putative similar to
SP|Q9Y295 Developmentally regulated GTP-binding protein
1 (DRG 1) {Homo sapiens}; contains Pfam profiles
PF02824: TGS domain, PF01018: GTP1/OBG family
Length = 369
Score = 121 bits (291), Expect = 5e-28
Identities = 68/128 (53%), Positives = 75/128 (58%), Gaps = 2/128 (1%)
Frame = +1
Query: 256 LGLLKAKLAKYRSQLLEPSKKGGD--KGEGFDVLKSGDARVALIGFPSVGKXXXXXXXXX 429
LGLLKAKLAK R LL P KGG GEGFDV KSGD+RV L+GFPSVGK
Sbjct: 28 LGLLKAKLAKLRRDLLAPPTKGGGGGAGEGFDVTKSGDSRVGLVGFPSVGKSTLLNKLTG 87
Query: 430 XXXEAASYEFTTLTCIPGVIEYRGANNNC*ICXXXXXXXXXXXXXXXQGIAVARTADLVL 609
E ASYEFTTLTCIPGVI YRGA Q I+ ART + +L
Sbjct: 88 TFSEVASYEFTTLTCIPGVITYRGAKIQLLDLPGIIEGAKDGKGRGRQVISTARTCNCIL 147
Query: 610 MMLDATKP 633
++LDA KP
Sbjct: 148 IVLDAIKP 155
>At1g17470.1 68414.m02143 developmentally regulated GTP-binding
protein (DRG1) identical to developmentally regulated
GTP binding protein (DRG1) [Arabidopsis thaliana]
GI:2345150
Length = 399
Score = 117 bits (282), Expect = 6e-27
Identities = 66/125 (52%), Positives = 74/125 (59%)
Frame = +1
Query: 256 LGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVALIGFPSVGKXXXXXXXXXXX 435
LG LKAK+AK R+QLLEP K GEGF+V K G RVALIGFPSVGK
Sbjct: 27 LGQLKAKIAKLRTQLLEPPKGASGGGEGFEVTKYGHGRVALIGFPSVGKSTLLTMLTGTH 86
Query: 436 XEAASYEFTTLTCIPGVIEYRGANNNC*ICXXXXXXXXXXXXXXXQGIAVARTADLVLMM 615
EAASYEFTTLTCIPGVI Y Q IAVA+++DLVLM+
Sbjct: 87 SEAASYEFTTLTCIPGVIHYNDTKIQLLDLPGIIEGASEGKGRGRQVIAVAKSSDLVLMV 146
Query: 616 LDATK 630
LDA+K
Sbjct: 147 LDASK 151
>At1g72660.1 68414.m08403 developmentally regulated GTP-binding
protein, putative very strong similarity to
developmentally regulated GTP binding protein (DRG1)
[Arabidopsis thaliana] GI:2345150
Length = 399
Score = 116 bits (279), Expect = 1e-26
Identities = 65/125 (52%), Positives = 74/125 (59%)
Frame = +1
Query: 256 LGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVALIGFPSVGKXXXXXXXXXXX 435
LG LKAK+AK R+QLLEP K G+GF+V K G RVALIGFPSVGK
Sbjct: 27 LGQLKAKIAKLRTQLLEPPKGSSGGGDGFEVTKYGHGRVALIGFPSVGKSTLLTMLTGTH 86
Query: 436 XEAASYEFTTLTCIPGVIEYRGANNNC*ICXXXXXXXXXXXXXXXQGIAVARTADLVLMM 615
EAASYEFTTLTCIPGVI Y Q IAVA+++DLVLM+
Sbjct: 87 SEAASYEFTTLTCIPGVIHYNDTKIQLLDLPGIIEGASEGKGRGRQVIAVAKSSDLVLMV 146
Query: 616 LDATK 630
LDA+K
Sbjct: 147 LDASK 151
>At5g43060.1 68418.m05256 cysteine proteinase, putative / thiol
protease, putative similar to cysteine proteinase RD21A
precursor (thiol protease) GI:435619, SP:P43297 from
[Arabidopsis thaliana]
Length = 463
Score = 28.3 bits (60), Expect = 4.5
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = -3
Query: 383 PIKATRASPDFSTSKPSPLSPPFFEGSSNCDLYFA 279
PIK + P+ S PSP+ PP + CD YF+
Sbjct: 352 PIKKGQNPPNPGPSPPSPIKPP-----TTCDKYFS 381
>At4g31630.1 68417.m04493 transcriptional factor B3 family protein
similar to reproductive meristem gene 1 from [Brassica
oleracea var. botrytis] GI:3170424, [Arabidopsis
thaliana] GI:13604227; contains Pfam profile PF02362: B3
DNA binding domain
Length = 512
Score = 27.9 bits (59), Expect = 6.0
Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = -3
Query: 422 RVDKRVDLPTEGKPIKATRASP-DFSTSKPSPLS 324
RV+KR + EG P ++TRA P + +P +S
Sbjct: 435 RVEKRAQVTEEGGPSRSTRADPGNLQQKQPCSIS 468
>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 545
Score = 27.9 bits (59), Expect = 6.0
Identities = 15/49 (30%), Positives = 23/49 (46%)
Frame = +3
Query: 411 FIDPHSHTFRSSKL*IHNTHMYTWSYRISRCQQQLLDLPGIIEGAAQGQ 557
F++ + F S +H T ++TWS SRC + L G +GQ
Sbjct: 19 FLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSASLGINRGQ 67
>At4g31490.1 68417.m04473 coatomer beta subunit, putative /
beta-coat protein, putative / beta-COP, putative similar
to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
from {Rattus norvegicus} SP|P23514, {Mus musculus}
SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 948
Score = 27.5 bits (58), Expect = 7.9
Identities = 14/42 (33%), Positives = 24/42 (57%)
Frame = -1
Query: 394 QKESLSKQLEHHQTLVHRNLHLCLHLFLKVPATVIYILLIWL 269
Q L K E+ Q L+ +H C F +V +TV+++L+ +L
Sbjct: 364 QSGELEKNGEYRQMLIQA-IHACAVKFPEVASTVVHLLMDFL 404
>At4g31480.1 68417.m04472 coatomer beta subunit, putative /
beta-coat protein, putative / beta-COP, putative similar
to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
from {Rattus norvegicus} SP|P23514, {Mus musculus}
SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 971
Score = 27.5 bits (58), Expect = 7.9
Identities = 14/42 (33%), Positives = 24/42 (57%)
Frame = -1
Query: 394 QKESLSKQLEHHQTLVHRNLHLCLHLFLKVPATVIYILLIWL 269
Q L K E+ Q L+ +H C F +V +TV+++L+ +L
Sbjct: 387 QSGELEKNGEYRQMLIQA-IHACAVKFPEVASTVVHLLMDFL 427
>At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative
Length = 953
Score = 27.5 bits (58), Expect = 7.9
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +1
Query: 256 LGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVAL 378
L ++ K KY LL K DK E + +SG+ ++ L
Sbjct: 787 LEIVTKKFPKYNCGLLHGRMKSDDKEEALNKFRSGETQILL 827
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,552,715
Number of Sequences: 28952
Number of extensions: 212778
Number of successful extensions: 562
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 554
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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